Molecular and phylogenetic characterization of Theileria spp. parasites in autochthonous bovines (Mirandesa breed) in Portugal
A survey was conducted during the months of April–June 2003 in the northeast Portugal (Bragança district) in order to characterize the hemoparasite population of an autochthonous Mirandesa breed of Bos taurus. The polymerase chain reaction (PCR) analysis of the bovine blood revealed that 3 out of 11...
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| Veröffentlicht in: | Veterinary parasitology 2004-08, Vol.123 (1), p.17-23 |
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| creator | Brı́gido, Clarisse da Fonseca, Isabel Pereira Parreira, Ricardo Fazendeiro, Isabel do Rosário, Virgı́lio E Centeno-Lima, Sónia |
| description | A survey was conducted during the months of April–June 2003 in the northeast Portugal (Bragança district) in order to characterize the hemoparasite population of an autochthonous Mirandesa breed of
Bos taurus. The polymerase chain reaction (PCR) analysis of the bovine blood revealed that 3 out of 116 animals were infected with
Theileria and/or
Babesia parasites, while reverse line blot hybridisation (RLB) analysis showed that these animals were infected with
Theileria buffeli/
orientalis. Cloning and sequencing confirmed the RLB results. Database sequence searches combined with phylogenetic analysis of the partial 18S ribosomal RNA gene sequences obtained enabled us to place the parasites in question as members of the
T. buffeli/
orientalis group, confirming the PCR/RLB diagnosis. |
| doi_str_mv | 10.1016/j.vetpar.2004.05.024 |
| format | Article |
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Bos taurus. The polymerase chain reaction (PCR) analysis of the bovine blood revealed that 3 out of 116 animals were infected with
Theileria and/or
Babesia parasites, while reverse line blot hybridisation (RLB) analysis showed that these animals were infected with
Theileria buffeli/
orientalis. Cloning and sequencing confirmed the RLB results. Database sequence searches combined with phylogenetic analysis of the partial 18S ribosomal RNA gene sequences obtained enabled us to place the parasites in question as members of the
T. buffeli/
orientalis group, confirming the PCR/RLB diagnosis.</description><identifier>ISSN: 0304-4017</identifier><identifier>EISSN: 1873-2550</identifier><identifier>DOI: 10.1016/j.vetpar.2004.05.024</identifier><identifier>PMID: 15265567</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; Babesia ; Babesia - genetics ; Babesia - isolation & purification ; babesiosis ; Babesiosis - blood ; Babesiosis - parasitology ; Babesiosis - veterinary ; Base Sequence ; Bos taurus ; Bovine Mirandesa breed ; Cattle ; cattle breeds ; Cattle Diseases - blood ; Cattle Diseases - parasitology ; disease prevalence ; DNA, Protozoan - chemistry ; DNA, Protozoan - genetics ; epidemiological studies ; Molecular Sequence Data ; Nucleic Acid Hybridization ; nucleotide sequences ; pathogen identification ; Phylogenetic analysis ; Phylogeny ; Polymerase Chain Reaction - veterinary ; Portugal ; ribosomal DNA ; RLB ; RNA, Ribosomal, 18S - chemistry ; RNA, Ribosomal, 18S - genetics ; Sequence Alignment ; Theileria ; Theileria - genetics ; Theileria - isolation & purification ; Theileria buffeli ; Theileria buffeli/orientalis group ; Theileriasis - blood ; Theileriasis - parasitology ; theileriosis</subject><ispartof>Veterinary parasitology, 2004-08, Vol.123 (1), p.17-23</ispartof><rights>2004 Elsevier B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c479t-34dd402bacc0f73f9faeff9373b5ad8d86b47e92258d5c061b4008785429015d3</citedby><cites>FETCH-LOGICAL-c479t-34dd402bacc0f73f9faeff9373b5ad8d86b47e92258d5c061b4008785429015d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0304401704002419$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15265567$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Brı́gido, Clarisse</creatorcontrib><creatorcontrib>da Fonseca, Isabel Pereira</creatorcontrib><creatorcontrib>Parreira, Ricardo</creatorcontrib><creatorcontrib>Fazendeiro, Isabel</creatorcontrib><creatorcontrib>do Rosário, Virgı́lio E</creatorcontrib><creatorcontrib>Centeno-Lima, Sónia</creatorcontrib><title>Molecular and phylogenetic characterization of Theileria spp. parasites in autochthonous bovines (Mirandesa breed) in Portugal</title><title>Veterinary parasitology</title><addtitle>Vet Parasitol</addtitle><description>A survey was conducted during the months of April–June 2003 in the northeast Portugal (Bragança district) in order to characterize the hemoparasite population of an autochthonous Mirandesa breed of
Bos taurus. The polymerase chain reaction (PCR) analysis of the bovine blood revealed that 3 out of 116 animals were infected with
Theileria and/or
Babesia parasites, while reverse line blot hybridisation (RLB) analysis showed that these animals were infected with
Theileria buffeli/
orientalis. Cloning and sequencing confirmed the RLB results. Database sequence searches combined with phylogenetic analysis of the partial 18S ribosomal RNA gene sequences obtained enabled us to place the parasites in question as members of the
T. buffeli/
orientalis group, confirming the PCR/RLB diagnosis.</description><subject>Animals</subject><subject>Babesia</subject><subject>Babesia - genetics</subject><subject>Babesia - isolation & purification</subject><subject>babesiosis</subject><subject>Babesiosis - blood</subject><subject>Babesiosis - parasitology</subject><subject>Babesiosis - veterinary</subject><subject>Base Sequence</subject><subject>Bos taurus</subject><subject>Bovine Mirandesa breed</subject><subject>Cattle</subject><subject>cattle breeds</subject><subject>Cattle Diseases - blood</subject><subject>Cattle Diseases - parasitology</subject><subject>disease prevalence</subject><subject>DNA, Protozoan - chemistry</subject><subject>DNA, Protozoan - genetics</subject><subject>epidemiological studies</subject><subject>Molecular Sequence Data</subject><subject>Nucleic Acid Hybridization</subject><subject>nucleotide sequences</subject><subject>pathogen identification</subject><subject>Phylogenetic analysis</subject><subject>Phylogeny</subject><subject>Polymerase Chain Reaction - veterinary</subject><subject>Portugal</subject><subject>ribosomal DNA</subject><subject>RLB</subject><subject>RNA, Ribosomal, 18S - chemistry</subject><subject>RNA, Ribosomal, 18S - genetics</subject><subject>Sequence Alignment</subject><subject>Theileria</subject><subject>Theileria - genetics</subject><subject>Theileria - isolation & purification</subject><subject>Theileria buffeli</subject><subject>Theileria buffeli/orientalis group</subject><subject>Theileriasis - blood</subject><subject>Theileriasis - parasitology</subject><subject>theileriosis</subject><issn>0304-4017</issn><issn>1873-2550</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkV2L1DAUhoso7rj6D0RzJXox9aRJmvZmQRa_YBcFd69DmpxOM3SamqQD64W_3Qwd8E6vAslz3rycpyheUigp0Pr9vjximnUoKwBegiih4o-KDW0k21ZCwONiAwz4lgOVF8WzGPeQQajl0-KCiqoWopab4vetH9Esow5ET5bMw8PodzhhcoaYQQdtEgb3SyfnJ-J7cjegG_ONJnGeS5L_19EljMRNRC_JmyENfvJLJJ0_uik_vL11IUdj1KQLiPbdCf3uQ1p2enxePOn1GPHF-bws7j99vLv-sr359vnr9YebreGyTVvGreVQddoY6CXr215j37dMsk5o29im7rjEtqpEY4WBmnYcoJGN4FULVFh2WbxZc-fgfy4Ykzq4aHAc9YS5rKpryWhdN_8FaSvatqUig3wFTfAxBuzVHNxBhwdFQZ0Eqb1aBamTIAVCZUF57NU5f-kOaP8OnY1k4PUK9NorvQsuqvsfFVAG0HLOxKni1UpgXtjRYVDROJwMWhfQJGW9-3eHP-XZruw</recordid><startdate>20040813</startdate><enddate>20040813</enddate><creator>Brı́gido, Clarisse</creator><creator>da Fonseca, Isabel Pereira</creator><creator>Parreira, Ricardo</creator><creator>Fazendeiro, Isabel</creator><creator>do Rosário, Virgı́lio E</creator><creator>Centeno-Lima, Sónia</creator><general>Elsevier B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20040813</creationdate><title>Molecular and phylogenetic characterization of Theileria spp. parasites in autochthonous bovines (Mirandesa breed) in Portugal</title><author>Brı́gido, Clarisse ; da Fonseca, Isabel Pereira ; Parreira, Ricardo ; Fazendeiro, Isabel ; do Rosário, Virgı́lio E ; Centeno-Lima, Sónia</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c479t-34dd402bacc0f73f9faeff9373b5ad8d86b47e92258d5c061b4008785429015d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Animals</topic><topic>Babesia</topic><topic>Babesia - genetics</topic><topic>Babesia - isolation & purification</topic><topic>babesiosis</topic><topic>Babesiosis - blood</topic><topic>Babesiosis - parasitology</topic><topic>Babesiosis - veterinary</topic><topic>Base Sequence</topic><topic>Bos taurus</topic><topic>Bovine Mirandesa breed</topic><topic>Cattle</topic><topic>cattle breeds</topic><topic>Cattle Diseases - blood</topic><topic>Cattle Diseases - parasitology</topic><topic>disease prevalence</topic><topic>DNA, Protozoan - chemistry</topic><topic>DNA, Protozoan - genetics</topic><topic>epidemiological studies</topic><topic>Molecular Sequence Data</topic><topic>Nucleic Acid Hybridization</topic><topic>nucleotide sequences</topic><topic>pathogen identification</topic><topic>Phylogenetic analysis</topic><topic>Phylogeny</topic><topic>Polymerase Chain Reaction - veterinary</topic><topic>Portugal</topic><topic>ribosomal DNA</topic><topic>RLB</topic><topic>RNA, Ribosomal, 18S - chemistry</topic><topic>RNA, Ribosomal, 18S - genetics</topic><topic>Sequence Alignment</topic><topic>Theileria</topic><topic>Theileria - genetics</topic><topic>Theileria - isolation & purification</topic><topic>Theileria buffeli</topic><topic>Theileria buffeli/orientalis group</topic><topic>Theileriasis - blood</topic><topic>Theileriasis - parasitology</topic><topic>theileriosis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Brı́gido, Clarisse</creatorcontrib><creatorcontrib>da Fonseca, Isabel Pereira</creatorcontrib><creatorcontrib>Parreira, Ricardo</creatorcontrib><creatorcontrib>Fazendeiro, Isabel</creatorcontrib><creatorcontrib>do Rosário, Virgı́lio E</creatorcontrib><creatorcontrib>Centeno-Lima, Sónia</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Veterinary parasitology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Brı́gido, Clarisse</au><au>da Fonseca, Isabel Pereira</au><au>Parreira, Ricardo</au><au>Fazendeiro, Isabel</au><au>do Rosário, Virgı́lio E</au><au>Centeno-Lima, Sónia</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular and phylogenetic characterization of Theileria spp. parasites in autochthonous bovines (Mirandesa breed) in Portugal</atitle><jtitle>Veterinary parasitology</jtitle><addtitle>Vet Parasitol</addtitle><date>2004-08-13</date><risdate>2004</risdate><volume>123</volume><issue>1</issue><spage>17</spage><epage>23</epage><pages>17-23</pages><issn>0304-4017</issn><eissn>1873-2550</eissn><abstract>A survey was conducted during the months of April–June 2003 in the northeast Portugal (Bragança district) in order to characterize the hemoparasite population of an autochthonous Mirandesa breed of
Bos taurus. The polymerase chain reaction (PCR) analysis of the bovine blood revealed that 3 out of 116 animals were infected with
Theileria and/or
Babesia parasites, while reverse line blot hybridisation (RLB) analysis showed that these animals were infected with
Theileria buffeli/
orientalis. Cloning and sequencing confirmed the RLB results. Database sequence searches combined with phylogenetic analysis of the partial 18S ribosomal RNA gene sequences obtained enabled us to place the parasites in question as members of the
T. buffeli/
orientalis group, confirming the PCR/RLB diagnosis.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>15265567</pmid><doi>10.1016/j.vetpar.2004.05.024</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Veterinary parasitology, 2004-08, Vol.123 (1), p.17-23 |
| issn | 0304-4017 1873-2550 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Babesia Babesia - genetics Babesia - isolation & purification babesiosis Babesiosis - blood Babesiosis - parasitology Babesiosis - veterinary Base Sequence Bos taurus Bovine Mirandesa breed Cattle cattle breeds Cattle Diseases - blood Cattle Diseases - parasitology disease prevalence DNA, Protozoan - chemistry DNA, Protozoan - genetics epidemiological studies Molecular Sequence Data Nucleic Acid Hybridization nucleotide sequences pathogen identification Phylogenetic analysis Phylogeny Polymerase Chain Reaction - veterinary Portugal ribosomal DNA RLB RNA, Ribosomal, 18S - chemistry RNA, Ribosomal, 18S - genetics Sequence Alignment Theileria Theileria - genetics Theileria - isolation & purification Theileria buffeli Theileria buffeli/orientalis group Theileriasis - blood Theileriasis - parasitology theileriosis |
| title | Molecular and phylogenetic characterization of Theileria spp. parasites in autochthonous bovines (Mirandesa breed) in Portugal |
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