Identification of polymorphism and association analysis with reproductive traits in the porcine RNF4 gene
The ring finger protein 4 gene ( RNF4), which might play a role in fetal germ cell development as well as in oocyte and granulosa cell maturation, was one of the potential candidate genes for reproductive traits. In the present work, we isolated the complete coding sequence of porcine RNF4 gene, ide...
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| Veröffentlicht in: | Animal reproduction science 2009-02, Vol.110 (3), p.283-292 |
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| creator | Niu, B.Y. Ye, L.Z. Li, F.E. Deng, C.Y. Jiang, S.W. Lei, M.G. Xiong, Y.Z. |
| description | The ring finger protein 4 gene (
RNF4), which might play a role in fetal germ cell development as well as in oocyte and granulosa cell maturation, was one of the potential candidate genes for reproductive traits. In the present work, we isolated the complete coding sequence of porcine
RNF4 gene, identified a single nucleotide polymorphism (SNP: T/C) in intron5, and developed a PCR-
SacII-RFLP genotyping assay. Association of this SNP with reproductive traits was assessed in three populations with diverse genetic backgrounds. One was Chinese Qingping sows. Another was consisted of crossbred sows derived from Landrace, Large White, Chinese Tongcheng and/or Chinese Meishan (Line DIV). The third is Large White
×
Meishan (LW
×
M) F
2 slaughtered population. Statistical analysis demonstrated that, in the first parity, the difference between
RNF4 genotypes and reproductive traits of both Qingping and Line DIV sows was not significant. In the second and subsequent litters, CC animals in Qingping population had more piglets born (+1.74 piglets) and piglets born alive (+2.02 piglets) than sows with the TT genotype (
P
<
0.05). Line DIV sows inheriting the CC genotype had additional 0.69 piglets born compared to the TC animals (
P
<
0.05) in second and subsequent litters. No significant difference was observed between genotypes and reproductive tracts components in F
2 animals. In addition, we found
RNF4 gene has a significant additive effect on both piglet born and piglet born alive in Qingping animals (
P
<
0.05). Results here suggested that the
RNF4 SNP was significantly associated with litter size in two populations and could be useful in selection for increasing litter size in pigs. Further studies were needed to confirm these preliminary researches. |
| doi_str_mv | 10.1016/j.anireprosci.2008.01.020 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_66728945</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0378432008000365</els_id><sourcerecordid>66728945</sourcerecordid><originalsourceid>FETCH-LOGICAL-c399t-62ed8ff9b4db59e3c40c840292a92200c55d0eece7f9dc64b405fc2542a67a723</originalsourceid><addsrcrecordid>eNqNkc1uEzEURi0EoqHwCmA27Ga4_hnPeIkiSitVIAFdW459p3E08QTbKcrb43YiwZKVFz73fp-PCXnPoGXA1Mdda2NIeEhzdqHlAEMLrAUOz8iKDb1oOBf8OVmB6IdGCg4X5FXOOwDoldIvyQUbRDcoqVYk3HiMJYzB2RLmSOeRHubptJ_TYRvyntroqc15dmG5t9FOpxwy_R3Klj518EdXwgPSkmwomYZIyxbrluRCRPr965Wk9xjxNXkx2injm_N5Se6uPv9cXze3377crD_dNk5oXRrF0Q_jqDfSbzqNwklwgwSuudW8PtV1nQdEh_2ovVNyI6EbHe8kt6q3PReX5MOyt1b7dcRczD5kh9NkI87HbJTq-aBlV0G9gK56zAlHc0hhb9PJMDCPns3O_OPZPHo2wEz1XGffnkOOmz36v5NnsRV4twCjnY29TyGbux8cmADW1T8AUYn1QmCV8RAwmRqC0aGvka4YP4f_KPIHh3GgVw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>66728945</pqid></control><display><type>article</type><title>Identification of polymorphism and association analysis with reproductive traits in the porcine RNF4 gene</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Niu, B.Y. ; Ye, L.Z. ; Li, F.E. ; Deng, C.Y. ; Jiang, S.W. ; Lei, M.G. ; Xiong, Y.Z.</creator><creatorcontrib>Niu, B.Y. ; Ye, L.Z. ; Li, F.E. ; Deng, C.Y. ; Jiang, S.W. ; Lei, M.G. ; Xiong, Y.Z.</creatorcontrib><description>The ring finger protein 4 gene (
RNF4), which might play a role in fetal germ cell development as well as in oocyte and granulosa cell maturation, was one of the potential candidate genes for reproductive traits. In the present work, we isolated the complete coding sequence of porcine
RNF4 gene, identified a single nucleotide polymorphism (SNP: T/C) in intron5, and developed a PCR-
SacII-RFLP genotyping assay. Association of this SNP with reproductive traits was assessed in three populations with diverse genetic backgrounds. One was Chinese Qingping sows. Another was consisted of crossbred sows derived from Landrace, Large White, Chinese Tongcheng and/or Chinese Meishan (Line DIV). The third is Large White
×
Meishan (LW
×
M) F
2 slaughtered population. Statistical analysis demonstrated that, in the first parity, the difference between
RNF4 genotypes and reproductive traits of both Qingping and Line DIV sows was not significant. In the second and subsequent litters, CC animals in Qingping population had more piglets born (+1.74 piglets) and piglets born alive (+2.02 piglets) than sows with the TT genotype (
P
<
0.05). Line DIV sows inheriting the CC genotype had additional 0.69 piglets born compared to the TC animals (
P
<
0.05) in second and subsequent litters. No significant difference was observed between genotypes and reproductive tracts components in F
2 animals. In addition, we found
RNF4 gene has a significant additive effect on both piglet born and piglet born alive in Qingping animals (
P
<
0.05). Results here suggested that the
RNF4 SNP was significantly associated with litter size in two populations and could be useful in selection for increasing litter size in pigs. Further studies were needed to confirm these preliminary researches.</description><identifier>ISSN: 0378-4320</identifier><identifier>EISSN: 1873-2232</identifier><identifier>DOI: 10.1016/j.anireprosci.2008.01.020</identifier><identifier>PMID: 18358646</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>additive gene effects ; Amino Acid Sequence ; animal genetics ; animal tissues ; Animals ; Animals, Newborn ; Base Sequence ; breed differences ; Crosses, Genetic ; Female ; female fertility ; gene expression ; Gene Expression Profiling - veterinary ; gene frequency ; genes ; genetic polymorphism ; genome ; genomics ; Genotype ; Litter size ; Litter Size - genetics ; molecular genetics ; Molecular Sequence Data ; mutation ; Nuclear Proteins - genetics ; Pig ; Polymorphism, Restriction Fragment Length ; Polymorphism, Single Nucleotide ; Reproduction - genetics ; reproductive traits ; Reverse Transcriptase Polymerase Chain Reaction - veterinary ; Ring finger protein 4 ; ring finger protein 4 gene ; RNA - chemistry ; RNA - genetics ; selection criteria ; Single nucleotide polymorphism ; sows ; Swine - genetics ; swine breeds ; Transcription Factors - genetics ; Zinc Fingers - genetics</subject><ispartof>Animal reproduction science, 2009-02, Vol.110 (3), p.283-292</ispartof><rights>2008 Elsevier B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c399t-62ed8ff9b4db59e3c40c840292a92200c55d0eece7f9dc64b405fc2542a67a723</citedby><cites>FETCH-LOGICAL-c399t-62ed8ff9b4db59e3c40c840292a92200c55d0eece7f9dc64b405fc2542a67a723</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0378432008000365$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18358646$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Niu, B.Y.</creatorcontrib><creatorcontrib>Ye, L.Z.</creatorcontrib><creatorcontrib>Li, F.E.</creatorcontrib><creatorcontrib>Deng, C.Y.</creatorcontrib><creatorcontrib>Jiang, S.W.</creatorcontrib><creatorcontrib>Lei, M.G.</creatorcontrib><creatorcontrib>Xiong, Y.Z.</creatorcontrib><title>Identification of polymorphism and association analysis with reproductive traits in the porcine RNF4 gene</title><title>Animal reproduction science</title><addtitle>Anim Reprod Sci</addtitle><description>The ring finger protein 4 gene (
RNF4), which might play a role in fetal germ cell development as well as in oocyte and granulosa cell maturation, was one of the potential candidate genes for reproductive traits. In the present work, we isolated the complete coding sequence of porcine
RNF4 gene, identified a single nucleotide polymorphism (SNP: T/C) in intron5, and developed a PCR-
SacII-RFLP genotyping assay. Association of this SNP with reproductive traits was assessed in three populations with diverse genetic backgrounds. One was Chinese Qingping sows. Another was consisted of crossbred sows derived from Landrace, Large White, Chinese Tongcheng and/or Chinese Meishan (Line DIV). The third is Large White
×
Meishan (LW
×
M) F
2 slaughtered population. Statistical analysis demonstrated that, in the first parity, the difference between
RNF4 genotypes and reproductive traits of both Qingping and Line DIV sows was not significant. In the second and subsequent litters, CC animals in Qingping population had more piglets born (+1.74 piglets) and piglets born alive (+2.02 piglets) than sows with the TT genotype (
P
<
0.05). Line DIV sows inheriting the CC genotype had additional 0.69 piglets born compared to the TC animals (
P
<
0.05) in second and subsequent litters. No significant difference was observed between genotypes and reproductive tracts components in F
2 animals. In addition, we found
RNF4 gene has a significant additive effect on both piglet born and piglet born alive in Qingping animals (
P
<
0.05). Results here suggested that the
RNF4 SNP was significantly associated with litter size in two populations and could be useful in selection for increasing litter size in pigs. Further studies were needed to confirm these preliminary researches.</description><subject>additive gene effects</subject><subject>Amino Acid Sequence</subject><subject>animal genetics</subject><subject>animal tissues</subject><subject>Animals</subject><subject>Animals, Newborn</subject><subject>Base Sequence</subject><subject>breed differences</subject><subject>Crosses, Genetic</subject><subject>Female</subject><subject>female fertility</subject><subject>gene expression</subject><subject>Gene Expression Profiling - veterinary</subject><subject>gene frequency</subject><subject>genes</subject><subject>genetic polymorphism</subject><subject>genome</subject><subject>genomics</subject><subject>Genotype</subject><subject>Litter size</subject><subject>Litter Size - genetics</subject><subject>molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>mutation</subject><subject>Nuclear Proteins - genetics</subject><subject>Pig</subject><subject>Polymorphism, Restriction Fragment Length</subject><subject>Polymorphism, Single Nucleotide</subject><subject>Reproduction - genetics</subject><subject>reproductive traits</subject><subject>Reverse Transcriptase Polymerase Chain Reaction - veterinary</subject><subject>Ring finger protein 4</subject><subject>ring finger protein 4 gene</subject><subject>RNA - chemistry</subject><subject>RNA - genetics</subject><subject>selection criteria</subject><subject>Single nucleotide polymorphism</subject><subject>sows</subject><subject>Swine - genetics</subject><subject>swine breeds</subject><subject>Transcription Factors - genetics</subject><subject>Zinc Fingers - genetics</subject><issn>0378-4320</issn><issn>1873-2232</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc1uEzEURi0EoqHwCmA27Ga4_hnPeIkiSitVIAFdW459p3E08QTbKcrb43YiwZKVFz73fp-PCXnPoGXA1Mdda2NIeEhzdqHlAEMLrAUOz8iKDb1oOBf8OVmB6IdGCg4X5FXOOwDoldIvyQUbRDcoqVYk3HiMJYzB2RLmSOeRHubptJ_TYRvyntroqc15dmG5t9FOpxwy_R3Klj518EdXwgPSkmwomYZIyxbrluRCRPr965Wk9xjxNXkx2injm_N5Se6uPv9cXze3377crD_dNk5oXRrF0Q_jqDfSbzqNwklwgwSuudW8PtV1nQdEh_2ovVNyI6EbHe8kt6q3PReX5MOyt1b7dcRczD5kh9NkI87HbJTq-aBlV0G9gK56zAlHc0hhb9PJMDCPns3O_OPZPHo2wEz1XGffnkOOmz36v5NnsRV4twCjnY29TyGbux8cmADW1T8AUYn1QmCV8RAwmRqC0aGvka4YP4f_KPIHh3GgVw</recordid><startdate>20090201</startdate><enddate>20090201</enddate><creator>Niu, B.Y.</creator><creator>Ye, L.Z.</creator><creator>Li, F.E.</creator><creator>Deng, C.Y.</creator><creator>Jiang, S.W.</creator><creator>Lei, M.G.</creator><creator>Xiong, Y.Z.</creator><general>Elsevier B.V</general><general>[Amsterdam]: Elsevier Science</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20090201</creationdate><title>Identification of polymorphism and association analysis with reproductive traits in the porcine RNF4 gene</title><author>Niu, B.Y. ; Ye, L.Z. ; Li, F.E. ; Deng, C.Y. ; Jiang, S.W. ; Lei, M.G. ; Xiong, Y.Z.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c399t-62ed8ff9b4db59e3c40c840292a92200c55d0eece7f9dc64b405fc2542a67a723</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>additive gene effects</topic><topic>Amino Acid Sequence</topic><topic>animal genetics</topic><topic>animal tissues</topic><topic>Animals</topic><topic>Animals, Newborn</topic><topic>Base Sequence</topic><topic>breed differences</topic><topic>Crosses, Genetic</topic><topic>Female</topic><topic>female fertility</topic><topic>gene expression</topic><topic>Gene Expression Profiling - veterinary</topic><topic>gene frequency</topic><topic>genes</topic><topic>genetic polymorphism</topic><topic>genome</topic><topic>genomics</topic><topic>Genotype</topic><topic>Litter size</topic><topic>Litter Size - genetics</topic><topic>molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>mutation</topic><topic>Nuclear Proteins - genetics</topic><topic>Pig</topic><topic>Polymorphism, Restriction Fragment Length</topic><topic>Polymorphism, Single Nucleotide</topic><topic>Reproduction - genetics</topic><topic>reproductive traits</topic><topic>Reverse Transcriptase Polymerase Chain Reaction - veterinary</topic><topic>Ring finger protein 4</topic><topic>ring finger protein 4 gene</topic><topic>RNA - chemistry</topic><topic>RNA - genetics</topic><topic>selection criteria</topic><topic>Single nucleotide polymorphism</topic><topic>sows</topic><topic>Swine - genetics</topic><topic>swine breeds</topic><topic>Transcription Factors - genetics</topic><topic>Zinc Fingers - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Niu, B.Y.</creatorcontrib><creatorcontrib>Ye, L.Z.</creatorcontrib><creatorcontrib>Li, F.E.</creatorcontrib><creatorcontrib>Deng, C.Y.</creatorcontrib><creatorcontrib>Jiang, S.W.</creatorcontrib><creatorcontrib>Lei, M.G.</creatorcontrib><creatorcontrib>Xiong, Y.Z.</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Animal reproduction science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Niu, B.Y.</au><au>Ye, L.Z.</au><au>Li, F.E.</au><au>Deng, C.Y.</au><au>Jiang, S.W.</au><au>Lei, M.G.</au><au>Xiong, Y.Z.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of polymorphism and association analysis with reproductive traits in the porcine RNF4 gene</atitle><jtitle>Animal reproduction science</jtitle><addtitle>Anim Reprod Sci</addtitle><date>2009-02-01</date><risdate>2009</risdate><volume>110</volume><issue>3</issue><spage>283</spage><epage>292</epage><pages>283-292</pages><issn>0378-4320</issn><eissn>1873-2232</eissn><abstract>The ring finger protein 4 gene (
RNF4), which might play a role in fetal germ cell development as well as in oocyte and granulosa cell maturation, was one of the potential candidate genes for reproductive traits. In the present work, we isolated the complete coding sequence of porcine
RNF4 gene, identified a single nucleotide polymorphism (SNP: T/C) in intron5, and developed a PCR-
SacII-RFLP genotyping assay. Association of this SNP with reproductive traits was assessed in three populations with diverse genetic backgrounds. One was Chinese Qingping sows. Another was consisted of crossbred sows derived from Landrace, Large White, Chinese Tongcheng and/or Chinese Meishan (Line DIV). The third is Large White
×
Meishan (LW
×
M) F
2 slaughtered population. Statistical analysis demonstrated that, in the first parity, the difference between
RNF4 genotypes and reproductive traits of both Qingping and Line DIV sows was not significant. In the second and subsequent litters, CC animals in Qingping population had more piglets born (+1.74 piglets) and piglets born alive (+2.02 piglets) than sows with the TT genotype (
P
<
0.05). Line DIV sows inheriting the CC genotype had additional 0.69 piglets born compared to the TC animals (
P
<
0.05) in second and subsequent litters. No significant difference was observed between genotypes and reproductive tracts components in F
2 animals. In addition, we found
RNF4 gene has a significant additive effect on both piglet born and piglet born alive in Qingping animals (
P
<
0.05). Results here suggested that the
RNF4 SNP was significantly associated with litter size in two populations and could be useful in selection for increasing litter size in pigs. Further studies were needed to confirm these preliminary researches.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>18358646</pmid><doi>10.1016/j.anireprosci.2008.01.020</doi><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Animal reproduction science, 2009-02, Vol.110 (3), p.283-292 |
| issn | 0378-4320 1873-2232 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_66728945 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | additive gene effects Amino Acid Sequence animal genetics animal tissues Animals Animals, Newborn Base Sequence breed differences Crosses, Genetic Female female fertility gene expression Gene Expression Profiling - veterinary gene frequency genes genetic polymorphism genome genomics Genotype Litter size Litter Size - genetics molecular genetics Molecular Sequence Data mutation Nuclear Proteins - genetics Pig Polymorphism, Restriction Fragment Length Polymorphism, Single Nucleotide Reproduction - genetics reproductive traits Reverse Transcriptase Polymerase Chain Reaction - veterinary Ring finger protein 4 ring finger protein 4 gene RNA - chemistry RNA - genetics selection criteria Single nucleotide polymorphism sows Swine - genetics swine breeds Transcription Factors - genetics Zinc Fingers - genetics |
| title | Identification of polymorphism and association analysis with reproductive traits in the porcine RNF4 gene |
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