Metabolic pathways for dietary lipids in the midgut of hematophagous Panstrongylus megistus (Hemiptera: Reduviidae)
The metabolism of dietary lipids in the anterior midgut of Panstrongylus megistus during blood digestion was studied. Fifth instar nymphs were fed a blood meal containing 7.1±0.4 mg of lipids, consisting mainly of triacylglycerol (TAG), and completed the overall process of digestion in about 20 days...
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| Veröffentlicht in: | Insect biochemistry and molecular biology 2004-08, Vol.34 (8), p.845-854 |
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| creator | Canavoso, Lilián E Frede, Silvia Rubiolo, Edilberto R |
| description | The metabolism of dietary lipids in the anterior midgut of
Panstrongylus megistus during blood digestion was studied. Fifth instar nymphs were fed a blood meal containing 7.1±0.4 mg of lipids, consisting mainly of triacylglycerol (TAG), and completed the overall process of digestion in about 20 days. Lipolysis of TAG and pathways for diacylglycerol (DAG) biosynthesis in the midgut were investigated by feeding the insects with [9,10-
3H]-oleic acid-labeled triolein. Lumenal [
3H]-triacylglycerol was hydrolyzed, generating mainly fatty acids (FA) and glycerol and to lesser extent, DAG. Almost no radioactivity associated with monoacylglycerol was found at any time. In midgut tissue, labeled fatty acids were incorporated into phosphatidic acid, DAG and TAG, whereas no significantly labeled monoacylglycerol was observed. In addition, the activities of enzymes related to DAG metabolism were assayed in non-blood fed midgut homogenates and at different times after feeding on a blood meal. Significant changes in the activities of phosphatidate phosphohydrolase (EC 3.1.3.4) and triacylglycerol lipase (EC 3.1.1.3) were observed during blood digestion, suggesting that these enzymes are important in regulating intracellular DAG synthesis and mobilization in midgut cells. Finally, the histological changes of lipid stores observed in anterior midgut confirmed the active process of uptake and trafficking of lipids performed by the enterocytes during blood digestion. |
| doi_str_mv | 10.1016/j.ibmb.2004.05.008 |
| format | Article |
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Panstrongylus megistus during blood digestion was studied. Fifth instar nymphs were fed a blood meal containing 7.1±0.4 mg of lipids, consisting mainly of triacylglycerol (TAG), and completed the overall process of digestion in about 20 days. Lipolysis of TAG and pathways for diacylglycerol (DAG) biosynthesis in the midgut were investigated by feeding the insects with [9,10-
3H]-oleic acid-labeled triolein. Lumenal [
3H]-triacylglycerol was hydrolyzed, generating mainly fatty acids (FA) and glycerol and to lesser extent, DAG. Almost no radioactivity associated with monoacylglycerol was found at any time. In midgut tissue, labeled fatty acids were incorporated into phosphatidic acid, DAG and TAG, whereas no significantly labeled monoacylglycerol was observed. In addition, the activities of enzymes related to DAG metabolism were assayed in non-blood fed midgut homogenates and at different times after feeding on a blood meal. Significant changes in the activities of phosphatidate phosphohydrolase (EC 3.1.3.4) and triacylglycerol lipase (EC 3.1.1.3) were observed during blood digestion, suggesting that these enzymes are important in regulating intracellular DAG synthesis and mobilization in midgut cells. Finally, the histological changes of lipid stores observed in anterior midgut confirmed the active process of uptake and trafficking of lipids performed by the enterocytes during blood digestion.</description><identifier>ISSN: 0965-1748</identifier><identifier>EISSN: 1879-0240</identifier><identifier>DOI: 10.1016/j.ibmb.2004.05.008</identifier><identifier>PMID: 15262288</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Animals ; biochemical pathways ; blood digestion ; Blood meal ; Diacylglycerol ; dietary fat ; Dietary Fats - blood ; Dietary Fats - metabolism ; Dietary Fats - pharmacokinetics ; Dietary Fats - pharmacology ; digestive enzymes ; Digestive System - enzymology ; Digestive System - metabolism ; Diglycerides - biosynthesis ; enzyme activity ; fatty acids ; hematophagous insects ; hematophagy ; Lipase ; Lipase - metabolism ; Lipid Metabolism ; lipids ; Lipids - administration & dosage ; Lipids - classification ; Lipids - pharmacokinetics ; Midgut ; Nymph - enzymology ; Nymph - metabolism ; Panstrongylus - enzymology ; Panstrongylus - growth & development ; Panstrongylus - metabolism ; Panstrongylus megistus ; Phosphatidate Phosphatase - metabolism ; Phosphatidate phosphohydrolase ; Phosphatidic acid ; Reduviidae ; Triacylglycerol ; triacylglycerols</subject><ispartof>Insect biochemistry and molecular biology, 2004-08, Vol.34 (8), p.845-854</ispartof><rights>2004 Elsevier Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c407t-86273a2beee72f72bf56c6132c3c1572cefb9068434871844e19fbbf3fa1582b3</citedby><cites>FETCH-LOGICAL-c407t-86273a2beee72f72bf56c6132c3c1572cefb9068434871844e19fbbf3fa1582b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.ibmb.2004.05.008$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15262288$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Canavoso, Lilián E</creatorcontrib><creatorcontrib>Frede, Silvia</creatorcontrib><creatorcontrib>Rubiolo, Edilberto R</creatorcontrib><title>Metabolic pathways for dietary lipids in the midgut of hematophagous Panstrongylus megistus (Hemiptera: Reduviidae)</title><title>Insect biochemistry and molecular biology</title><addtitle>Insect Biochem Mol Biol</addtitle><description>The metabolism of dietary lipids in the anterior midgut of
Panstrongylus megistus during blood digestion was studied. Fifth instar nymphs were fed a blood meal containing 7.1±0.4 mg of lipids, consisting mainly of triacylglycerol (TAG), and completed the overall process of digestion in about 20 days. Lipolysis of TAG and pathways for diacylglycerol (DAG) biosynthesis in the midgut were investigated by feeding the insects with [9,10-
3H]-oleic acid-labeled triolein. Lumenal [
3H]-triacylglycerol was hydrolyzed, generating mainly fatty acids (FA) and glycerol and to lesser extent, DAG. Almost no radioactivity associated with monoacylglycerol was found at any time. In midgut tissue, labeled fatty acids were incorporated into phosphatidic acid, DAG and TAG, whereas no significantly labeled monoacylglycerol was observed. In addition, the activities of enzymes related to DAG metabolism were assayed in non-blood fed midgut homogenates and at different times after feeding on a blood meal. Significant changes in the activities of phosphatidate phosphohydrolase (EC 3.1.3.4) and triacylglycerol lipase (EC 3.1.1.3) were observed during blood digestion, suggesting that these enzymes are important in regulating intracellular DAG synthesis and mobilization in midgut cells. Finally, the histological changes of lipid stores observed in anterior midgut confirmed the active process of uptake and trafficking of lipids performed by the enterocytes during blood digestion.</description><subject>Animals</subject><subject>biochemical pathways</subject><subject>blood digestion</subject><subject>Blood meal</subject><subject>Diacylglycerol</subject><subject>dietary fat</subject><subject>Dietary Fats - blood</subject><subject>Dietary Fats - metabolism</subject><subject>Dietary Fats - pharmacokinetics</subject><subject>Dietary Fats - pharmacology</subject><subject>digestive enzymes</subject><subject>Digestive System - enzymology</subject><subject>Digestive System - metabolism</subject><subject>Diglycerides - biosynthesis</subject><subject>enzyme activity</subject><subject>fatty acids</subject><subject>hematophagous insects</subject><subject>hematophagy</subject><subject>Lipase</subject><subject>Lipase - metabolism</subject><subject>Lipid Metabolism</subject><subject>lipids</subject><subject>Lipids - administration & dosage</subject><subject>Lipids - classification</subject><subject>Lipids - pharmacokinetics</subject><subject>Midgut</subject><subject>Nymph - enzymology</subject><subject>Nymph - metabolism</subject><subject>Panstrongylus - enzymology</subject><subject>Panstrongylus - growth & development</subject><subject>Panstrongylus - metabolism</subject><subject>Panstrongylus megistus</subject><subject>Phosphatidate Phosphatase - metabolism</subject><subject>Phosphatidate phosphohydrolase</subject><subject>Phosphatidic acid</subject><subject>Reduviidae</subject><subject>Triacylglycerol</subject><subject>triacylglycerols</subject><issn>0965-1748</issn><issn>1879-0240</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU2P0zAQQCMEYsvCH-AAPiE4JIydxHYQF7QCFmkRCNizZTvj1FXSZG1nUf89rlqJG5zmQ29Go3lF8ZxCRYHyt7vKm8lUDKCpoK0A5INiQ6XoSmANPCw20PG2pKKRF8WTGHeQwaYVj4sL2jLOmJSbIn7FpM08eksWnba_9SESNwfS-9wPBzL6xfeR-D1JWyST74c1kdmRLU46zctWD_MayXe9jynM--Ew5mrCwceUk9fXOPklYdDvyA_s13vve41vnhaPnB4jPjvHy-L208dfV9flzbfPX64-3JS2AZFKyZmoNTOIKJgTzLiWW05rZmtLW8EsOtMBl03dSEFl0yDtnDGudpq2kpn6snh12ruE-W7FmNTko8Vx1HvMVyvOBRPQ1f8FqRDAO9ZmkJ1AG-YYAzq1BD_lPykK6uhE7dTRiTo6UdCq7CQPvThvX82E_d-Rs4QMvDwBTs9KD8FHdfuTAa0BuqYFEJl4fyIwv-veY1DRetxb7H1Am1Q_-39d8Ac1u6fs</recordid><startdate>20040801</startdate><enddate>20040801</enddate><creator>Canavoso, Lilián E</creator><creator>Frede, Silvia</creator><creator>Rubiolo, Edilberto R</creator><general>Elsevier Ltd</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SS</scope><scope>7X8</scope></search><sort><creationdate>20040801</creationdate><title>Metabolic pathways for dietary lipids in the midgut of hematophagous Panstrongylus megistus (Hemiptera: Reduviidae)</title><author>Canavoso, Lilián E ; Frede, Silvia ; Rubiolo, Edilberto R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c407t-86273a2beee72f72bf56c6132c3c1572cefb9068434871844e19fbbf3fa1582b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Animals</topic><topic>biochemical pathways</topic><topic>blood digestion</topic><topic>Blood meal</topic><topic>Diacylglycerol</topic><topic>dietary fat</topic><topic>Dietary Fats - blood</topic><topic>Dietary Fats - metabolism</topic><topic>Dietary Fats - pharmacokinetics</topic><topic>Dietary Fats - pharmacology</topic><topic>digestive enzymes</topic><topic>Digestive System - enzymology</topic><topic>Digestive System - metabolism</topic><topic>Diglycerides - biosynthesis</topic><topic>enzyme activity</topic><topic>fatty acids</topic><topic>hematophagous insects</topic><topic>hematophagy</topic><topic>Lipase</topic><topic>Lipase - metabolism</topic><topic>Lipid Metabolism</topic><topic>lipids</topic><topic>Lipids - administration & dosage</topic><topic>Lipids - classification</topic><topic>Lipids - pharmacokinetics</topic><topic>Midgut</topic><topic>Nymph - enzymology</topic><topic>Nymph - metabolism</topic><topic>Panstrongylus - enzymology</topic><topic>Panstrongylus - growth & development</topic><topic>Panstrongylus - metabolism</topic><topic>Panstrongylus megistus</topic><topic>Phosphatidate Phosphatase - metabolism</topic><topic>Phosphatidate phosphohydrolase</topic><topic>Phosphatidic acid</topic><topic>Reduviidae</topic><topic>Triacylglycerol</topic><topic>triacylglycerols</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Canavoso, Lilián E</creatorcontrib><creatorcontrib>Frede, Silvia</creatorcontrib><creatorcontrib>Rubiolo, Edilberto R</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Entomology Abstracts (Full archive)</collection><collection>MEDLINE - Academic</collection><jtitle>Insect biochemistry and molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Canavoso, Lilián E</au><au>Frede, Silvia</au><au>Rubiolo, Edilberto R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Metabolic pathways for dietary lipids in the midgut of hematophagous Panstrongylus megistus (Hemiptera: Reduviidae)</atitle><jtitle>Insect biochemistry and molecular biology</jtitle><addtitle>Insect Biochem Mol Biol</addtitle><date>2004-08-01</date><risdate>2004</risdate><volume>34</volume><issue>8</issue><spage>845</spage><epage>854</epage><pages>845-854</pages><issn>0965-1748</issn><eissn>1879-0240</eissn><abstract>The metabolism of dietary lipids in the anterior midgut of
Panstrongylus megistus during blood digestion was studied. Fifth instar nymphs were fed a blood meal containing 7.1±0.4 mg of lipids, consisting mainly of triacylglycerol (TAG), and completed the overall process of digestion in about 20 days. Lipolysis of TAG and pathways for diacylglycerol (DAG) biosynthesis in the midgut were investigated by feeding the insects with [9,10-
3H]-oleic acid-labeled triolein. Lumenal [
3H]-triacylglycerol was hydrolyzed, generating mainly fatty acids (FA) and glycerol and to lesser extent, DAG. Almost no radioactivity associated with monoacylglycerol was found at any time. In midgut tissue, labeled fatty acids were incorporated into phosphatidic acid, DAG and TAG, whereas no significantly labeled monoacylglycerol was observed. In addition, the activities of enzymes related to DAG metabolism were assayed in non-blood fed midgut homogenates and at different times after feeding on a blood meal. Significant changes in the activities of phosphatidate phosphohydrolase (EC 3.1.3.4) and triacylglycerol lipase (EC 3.1.1.3) were observed during blood digestion, suggesting that these enzymes are important in regulating intracellular DAG synthesis and mobilization in midgut cells. Finally, the histological changes of lipid stores observed in anterior midgut confirmed the active process of uptake and trafficking of lipids performed by the enterocytes during blood digestion.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>15262288</pmid><doi>10.1016/j.ibmb.2004.05.008</doi><tpages>10</tpages></addata></record> |
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| ispartof | Insect biochemistry and molecular biology, 2004-08, Vol.34 (8), p.845-854 |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Animals biochemical pathways blood digestion Blood meal Diacylglycerol dietary fat Dietary Fats - blood Dietary Fats - metabolism Dietary Fats - pharmacokinetics Dietary Fats - pharmacology digestive enzymes Digestive System - enzymology Digestive System - metabolism Diglycerides - biosynthesis enzyme activity fatty acids hematophagous insects hematophagy Lipase Lipase - metabolism Lipid Metabolism lipids Lipids - administration & dosage Lipids - classification Lipids - pharmacokinetics Midgut Nymph - enzymology Nymph - metabolism Panstrongylus - enzymology Panstrongylus - growth & development Panstrongylus - metabolism Panstrongylus megistus Phosphatidate Phosphatase - metabolism Phosphatidate phosphohydrolase Phosphatidic acid Reduviidae Triacylglycerol triacylglycerols |
| title | Metabolic pathways for dietary lipids in the midgut of hematophagous Panstrongylus megistus (Hemiptera: Reduviidae) |
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