Endogenously expressed HIV-1 nef down-regulates antigen-presenting molecules, not only class I MHC but also CD1a, in immature dendritic cells
The effects of Nef molecules on immature dendritic cells (iDCs) were analyzed using recombinant human immunodeficiency virus type 1 (HIV-1) with intact nef gene, pseudotyped with vesicular stomatitis virus glycoprotein, HIV/VSV-G/+Nef. When iDCs were infected with HIV/VSV-G/+Nef, the surface express...
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| Veröffentlicht in: | Virology (New York, N.Y.) N.Y.), 2004-08, Vol.326 (1), p.79-89 |
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| creator | Shinya, Eiji Owaki, Atsuko Shimizu, Masumi Takeuchi, Junko Kawashima, Tetsuo Hidaka, Chizuno Satomi, Misao Watari, Eiji Sugita, Masahiko Takahashi, Hidemi |
| description | The effects of Nef molecules on immature dendritic cells (iDCs) were analyzed using recombinant human immunodeficiency virus type 1 (HIV-1) with intact
nef gene, pseudotyped with vesicular stomatitis virus glycoprotein, HIV/VSV-G/+Nef. When iDCs were infected with HIV/VSV-G/+Nef, the surface expression of CD1a, a molecule for presenting glycolipid/lipid antigens, was selectively down-regulated among CD1 molecules (CD1a, -b, -c, and -d) as well as class I MHC. Moreover, the CD1a molecules were also down-modulated and co-localized with DsRed2-tagged-Nef in CD1a-transfected cells. Their co-localization was dependent upon CD1a cytoplasmic tail and the CD1a was redistributed from cell surface to LAMP-1
+ late endosomal/lysosomal compartment. These findings reveal that the HIV-1-Nef interferes with the intracellular trafficking of CD1a, and suggest the involvement of CD1a-restricted immune effectors in the protective immunity against HIV-1 infection, which implicates the feasibility of virus-derived glycolipid/lipid antigens together with epitope peptides for the vaccine development. |
| doi_str_mv | 10.1016/j.virol.2004.06.004 |
| format | Article |
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nef gene, pseudotyped with vesicular stomatitis virus glycoprotein, HIV/VSV-G/+Nef. When iDCs were infected with HIV/VSV-G/+Nef, the surface expression of CD1a, a molecule for presenting glycolipid/lipid antigens, was selectively down-regulated among CD1 molecules (CD1a, -b, -c, and -d) as well as class I MHC. Moreover, the CD1a molecules were also down-modulated and co-localized with DsRed2-tagged-Nef in CD1a-transfected cells. Their co-localization was dependent upon CD1a cytoplasmic tail and the CD1a was redistributed from cell surface to LAMP-1
+ late endosomal/lysosomal compartment. These findings reveal that the HIV-1-Nef interferes with the intracellular trafficking of CD1a, and suggest the involvement of CD1a-restricted immune effectors in the protective immunity against HIV-1 infection, which implicates the feasibility of virus-derived glycolipid/lipid antigens together with epitope peptides for the vaccine development.</description><identifier>ISSN: 0042-6822</identifier><identifier>EISSN: 1096-0341</identifier><identifier>DOI: 10.1016/j.virol.2004.06.004</identifier><identifier>PMID: 15262497</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Antigens, CD - immunology ; Antigens, CD1 - analysis ; Biological Transport ; CD1a ; Cell Line, Tumor ; Class I MHC ; Dendritic cells ; Dendritic Cells - immunology ; Dendritic Cells - virology ; Down-Regulation ; Endosomes - immunology ; Gene Products, nef - biosynthesis ; Gene Products, nef - genetics ; Gene Products, nef - physiology ; Glycoproteins ; Histocompatibility Antigens Class I - analysis ; HIV-1 ; HIV-1 - genetics ; HIV-1 - metabolism ; Human immunodeficiency virus 1 ; Humans ; Luminescent Proteins ; Lysosomal Membrane Proteins ; Lysosomes - immunology ; Nef ; nef Gene Products, Human Immunodeficiency Virus ; Transfection ; Vesicular stomatitis Indiana virus - genetics ; Vesicular stomatitis virus</subject><ispartof>Virology (New York, N.Y.), 2004-08, Vol.326 (1), p.79-89</ispartof><rights>2004 Elsevier Inc.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c386t-966a6d6ba77a6c8bfdf4f562599649f008c51de1e81f7f20eaa6e23c3e2dc8013</citedby><cites>FETCH-LOGICAL-c386t-966a6d6ba77a6c8bfdf4f562599649f008c51de1e81f7f20eaa6e23c3e2dc8013</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.virol.2004.06.004$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>315,781,785,3551,27929,27930,46000</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15262497$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shinya, Eiji</creatorcontrib><creatorcontrib>Owaki, Atsuko</creatorcontrib><creatorcontrib>Shimizu, Masumi</creatorcontrib><creatorcontrib>Takeuchi, Junko</creatorcontrib><creatorcontrib>Kawashima, Tetsuo</creatorcontrib><creatorcontrib>Hidaka, Chizuno</creatorcontrib><creatorcontrib>Satomi, Misao</creatorcontrib><creatorcontrib>Watari, Eiji</creatorcontrib><creatorcontrib>Sugita, Masahiko</creatorcontrib><creatorcontrib>Takahashi, Hidemi</creatorcontrib><title>Endogenously expressed HIV-1 nef down-regulates antigen-presenting molecules, not only class I MHC but also CD1a, in immature dendritic cells</title><title>Virology (New York, N.Y.)</title><addtitle>Virology</addtitle><description>The effects of Nef molecules on immature dendritic cells (iDCs) were analyzed using recombinant human immunodeficiency virus type 1 (HIV-1) with intact
nef gene, pseudotyped with vesicular stomatitis virus glycoprotein, HIV/VSV-G/+Nef. When iDCs were infected with HIV/VSV-G/+Nef, the surface expression of CD1a, a molecule for presenting glycolipid/lipid antigens, was selectively down-regulated among CD1 molecules (CD1a, -b, -c, and -d) as well as class I MHC. Moreover, the CD1a molecules were also down-modulated and co-localized with DsRed2-tagged-Nef in CD1a-transfected cells. Their co-localization was dependent upon CD1a cytoplasmic tail and the CD1a was redistributed from cell surface to LAMP-1
+ late endosomal/lysosomal compartment. These findings reveal that the HIV-1-Nef interferes with the intracellular trafficking of CD1a, and suggest the involvement of CD1a-restricted immune effectors in the protective immunity against HIV-1 infection, which implicates the feasibility of virus-derived glycolipid/lipid antigens together with epitope peptides for the vaccine development.</description><subject>Antigens, CD - immunology</subject><subject>Antigens, CD1 - analysis</subject><subject>Biological Transport</subject><subject>CD1a</subject><subject>Cell Line, Tumor</subject><subject>Class I MHC</subject><subject>Dendritic cells</subject><subject>Dendritic Cells - immunology</subject><subject>Dendritic Cells - virology</subject><subject>Down-Regulation</subject><subject>Endosomes - immunology</subject><subject>Gene Products, nef - biosynthesis</subject><subject>Gene Products, nef - genetics</subject><subject>Gene Products, nef - physiology</subject><subject>Glycoproteins</subject><subject>Histocompatibility Antigens Class I - analysis</subject><subject>HIV-1</subject><subject>HIV-1 - genetics</subject><subject>HIV-1 - metabolism</subject><subject>Human immunodeficiency virus 1</subject><subject>Humans</subject><subject>Luminescent Proteins</subject><subject>Lysosomal Membrane Proteins</subject><subject>Lysosomes - immunology</subject><subject>Nef</subject><subject>nef Gene Products, Human Immunodeficiency Virus</subject><subject>Transfection</subject><subject>Vesicular stomatitis Indiana virus - genetics</subject><subject>Vesicular stomatitis virus</subject><issn>0042-6822</issn><issn>1096-0341</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1vEzEQhi0EomnhFyAhnzh1F9u7a-8eOKC0JZGKeilcLceejRx57WB7-_Ej-M84TSRucJoPPfPOaF6EPlBSU0L55139YGNwNSOkrQmvS3iFFpQMvCJNS1-jRemwiveMnaHzlHak1EKQt-iMdoyzdhAL9Pvam7AFH-bknjE87SOkBAav1j8rij2M2IRHX0XYzk5lSFj5bAtfHUAoud_iKTjQs4N0iX3IOPiipJ1KCa_x99USb-aMlUsBL6-ousTWYztNKs8RsAFvos1WYw3OpXfozVhIeH-KF-jHzfX9clXd3n1bL7_eVrrpea4GzhU3fKOEUFz3m9GM7dhx1g0Db4eRkF531ACFno5iZASU4sAa3QAzuie0uUCfjrr7GH7NkLKcbDpcoDyUT0jOBeOiG_4LUsFEM5C2gM0R1DGkFGGU-2gnFZ8lJfJgl9zJF7vkwS5JuCQvUx9P8vNmAvN35uRPAb4cASjfeLAQZdIWvAZjI-gsTbD_XPAHP4qo3g</recordid><startdate>20040815</startdate><enddate>20040815</enddate><creator>Shinya, Eiji</creator><creator>Owaki, Atsuko</creator><creator>Shimizu, Masumi</creator><creator>Takeuchi, Junko</creator><creator>Kawashima, Tetsuo</creator><creator>Hidaka, Chizuno</creator><creator>Satomi, Misao</creator><creator>Watari, Eiji</creator><creator>Sugita, Masahiko</creator><creator>Takahashi, Hidemi</creator><general>Elsevier Inc</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>20040815</creationdate><title>Endogenously expressed HIV-1 nef down-regulates antigen-presenting molecules, not only class I MHC but also CD1a, in immature dendritic cells</title><author>Shinya, Eiji ; Owaki, Atsuko ; Shimizu, Masumi ; Takeuchi, Junko ; Kawashima, Tetsuo ; Hidaka, Chizuno ; Satomi, Misao ; Watari, Eiji ; Sugita, Masahiko ; Takahashi, Hidemi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c386t-966a6d6ba77a6c8bfdf4f562599649f008c51de1e81f7f20eaa6e23c3e2dc8013</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Antigens, CD - immunology</topic><topic>Antigens, CD1 - analysis</topic><topic>Biological Transport</topic><topic>CD1a</topic><topic>Cell Line, Tumor</topic><topic>Class I MHC</topic><topic>Dendritic cells</topic><topic>Dendritic Cells - immunology</topic><topic>Dendritic Cells - virology</topic><topic>Down-Regulation</topic><topic>Endosomes - immunology</topic><topic>Gene Products, nef - biosynthesis</topic><topic>Gene Products, nef - genetics</topic><topic>Gene Products, nef - physiology</topic><topic>Glycoproteins</topic><topic>Histocompatibility Antigens Class I - analysis</topic><topic>HIV-1</topic><topic>HIV-1 - genetics</topic><topic>HIV-1 - metabolism</topic><topic>Human immunodeficiency virus 1</topic><topic>Humans</topic><topic>Luminescent Proteins</topic><topic>Lysosomal Membrane Proteins</topic><topic>Lysosomes - immunology</topic><topic>Nef</topic><topic>nef Gene Products, Human Immunodeficiency Virus</topic><topic>Transfection</topic><topic>Vesicular stomatitis Indiana virus - genetics</topic><topic>Vesicular stomatitis virus</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shinya, Eiji</creatorcontrib><creatorcontrib>Owaki, Atsuko</creatorcontrib><creatorcontrib>Shimizu, Masumi</creatorcontrib><creatorcontrib>Takeuchi, Junko</creatorcontrib><creatorcontrib>Kawashima, Tetsuo</creatorcontrib><creatorcontrib>Hidaka, Chizuno</creatorcontrib><creatorcontrib>Satomi, Misao</creatorcontrib><creatorcontrib>Watari, Eiji</creatorcontrib><creatorcontrib>Sugita, Masahiko</creatorcontrib><creatorcontrib>Takahashi, Hidemi</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Virology (New York, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shinya, Eiji</au><au>Owaki, Atsuko</au><au>Shimizu, Masumi</au><au>Takeuchi, Junko</au><au>Kawashima, Tetsuo</au><au>Hidaka, Chizuno</au><au>Satomi, Misao</au><au>Watari, Eiji</au><au>Sugita, Masahiko</au><au>Takahashi, Hidemi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Endogenously expressed HIV-1 nef down-regulates antigen-presenting molecules, not only class I MHC but also CD1a, in immature dendritic cells</atitle><jtitle>Virology (New York, N.Y.)</jtitle><addtitle>Virology</addtitle><date>2004-08-15</date><risdate>2004</risdate><volume>326</volume><issue>1</issue><spage>79</spage><epage>89</epage><pages>79-89</pages><issn>0042-6822</issn><eissn>1096-0341</eissn><abstract>The effects of Nef molecules on immature dendritic cells (iDCs) were analyzed using recombinant human immunodeficiency virus type 1 (HIV-1) with intact
nef gene, pseudotyped with vesicular stomatitis virus glycoprotein, HIV/VSV-G/+Nef. When iDCs were infected with HIV/VSV-G/+Nef, the surface expression of CD1a, a molecule for presenting glycolipid/lipid antigens, was selectively down-regulated among CD1 molecules (CD1a, -b, -c, and -d) as well as class I MHC. Moreover, the CD1a molecules were also down-modulated and co-localized with DsRed2-tagged-Nef in CD1a-transfected cells. Their co-localization was dependent upon CD1a cytoplasmic tail and the CD1a was redistributed from cell surface to LAMP-1
+ late endosomal/lysosomal compartment. These findings reveal that the HIV-1-Nef interferes with the intracellular trafficking of CD1a, and suggest the involvement of CD1a-restricted immune effectors in the protective immunity against HIV-1 infection, which implicates the feasibility of virus-derived glycolipid/lipid antigens together with epitope peptides for the vaccine development.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>15262497</pmid><doi>10.1016/j.virol.2004.06.004</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Virology (New York, N.Y.), 2004-08, Vol.326 (1), p.79-89 |
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| source | MEDLINE; Access via ScienceDirect (Elsevier); EZB-FREE-00999 freely available EZB journals |
| subjects | Antigens, CD - immunology Antigens, CD1 - analysis Biological Transport CD1a Cell Line, Tumor Class I MHC Dendritic cells Dendritic Cells - immunology Dendritic Cells - virology Down-Regulation Endosomes - immunology Gene Products, nef - biosynthesis Gene Products, nef - genetics Gene Products, nef - physiology Glycoproteins Histocompatibility Antigens Class I - analysis HIV-1 HIV-1 - genetics HIV-1 - metabolism Human immunodeficiency virus 1 Humans Luminescent Proteins Lysosomal Membrane Proteins Lysosomes - immunology Nef nef Gene Products, Human Immunodeficiency Virus Transfection Vesicular stomatitis Indiana virus - genetics Vesicular stomatitis virus |
| title | Endogenously expressed HIV-1 nef down-regulates antigen-presenting molecules, not only class I MHC but also CD1a, in immature dendritic cells |
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