Gonadotropin Surge-Induced Differential Upregulation of Collagenase-1 (MMP-1) and Collagenase-3 (MMP-13) mRNA and Protein in Bovine Preovulatory Follicles
The ovulatory process is characterized by focalized extracellular matrix degradation at the apex of preovulatory follicles. Many studies have implicated the matrix metalloproteinases (MMPs) as potential mediators of follicle rupture. Objectives of this study were to determine localization and effect...
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| Veröffentlicht in: | Biology of reproduction 2004-08, Vol.71 (2), p.605-612 |
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| creator | BAKKE, Leanne J QINGLEI LI CASSAR, Carolyn A DOW, Mark P. D PURSLEY, J. Richard SMITH, George W |
| description | The ovulatory process is characterized by focalized extracellular matrix degradation at the apex of preovulatory follicles.
Many studies have implicated the matrix metalloproteinases (MMPs) as potential mediators of follicle rupture. Objectives of
this study were to determine localization and effect of the gonadotropin surge on temporal expression of MMP-1 and MMP-13
in bovine preovulatory follicles. Samples were collected at 0, 6, 12, 18, 24, and 48 h (corpora lutea) after GnRH injection
(n = 5â6 per time point) and amounts of MMP-1 and MMP-13 mRNA and protein determined using dot blot or semiquantitative RT-PCR
and Western blot analyses. Samples were also collected at 0 and 20 h after GnRH injection for immunohistochemical localization
of MMP-1 and MMP-13. Results indicate that follicular expression of MMP-1 and MMP-13 increased following the gonadotropin
surge. Abundance of MMP-1 mRNA increased at 6, 12, and 48 h post-GnRH injection. Immunoreactive MMP-1 was localized to granulosal
and thecal layers of preovulatory follicles. Amounts of MMP-1 protein increased in both the apex and the base of preovulatory
follicles. Abundance of MMP-13 mRNA increased at 6, 24, and 48 h post GnRH injection. Amounts of MMP-13 protein also increased
in the follicular apex and base. Immunoreactive MMP-13 was localized to granulosal and thecal layers of preovulatory follicles.
Results indicate MMP-1 and MMP-13 are increased in bovine preovulatory follicles following the gonadotropin surge but do not
support a requirement for differential up-regulation of MMP-1 and MMP-13 (follicular apex vs. base) for the preovulatory collagenolysis
required for follicle rupture. |
| doi_str_mv | 10.1095/biolreprod.104.027185 |
| format | Article |
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Many studies have implicated the matrix metalloproteinases (MMPs) as potential mediators of follicle rupture. Objectives of
this study were to determine localization and effect of the gonadotropin surge on temporal expression of MMP-1 and MMP-13
in bovine preovulatory follicles. Samples were collected at 0, 6, 12, 18, 24, and 48 h (corpora lutea) after GnRH injection
(n = 5â6 per time point) and amounts of MMP-1 and MMP-13 mRNA and protein determined using dot blot or semiquantitative RT-PCR
and Western blot analyses. Samples were also collected at 0 and 20 h after GnRH injection for immunohistochemical localization
of MMP-1 and MMP-13. Results indicate that follicular expression of MMP-1 and MMP-13 increased following the gonadotropin
surge. Abundance of MMP-1 mRNA increased at 6, 12, and 48 h post-GnRH injection. Immunoreactive MMP-1 was localized to granulosal
and thecal layers of preovulatory follicles. Amounts of MMP-1 protein increased in both the apex and the base of preovulatory
follicles. Abundance of MMP-13 mRNA increased at 6, 24, and 48 h post GnRH injection. Amounts of MMP-13 protein also increased
in the follicular apex and base. Immunoreactive MMP-13 was localized to granulosal and thecal layers of preovulatory follicles.
Results indicate MMP-1 and MMP-13 are increased in bovine preovulatory follicles following the gonadotropin surge but do not
support a requirement for differential up-regulation of MMP-1 and MMP-13 (follicular apex vs. base) for the preovulatory collagenolysis
required for follicle rupture.</description><identifier>ISSN: 0006-3363</identifier><identifier>EISSN: 1529-7268</identifier><identifier>DOI: 10.1095/biolreprod.104.027185</identifier><identifier>PMID: 15115716</identifier><identifier>CODEN: BIREBV</identifier><language>eng</language><publisher>Madison, WI: Society for the Study of Reproduction</publisher><subject>Animals ; Biological and medical sciences ; Cattle ; Collagenases - genetics ; Collagenases - metabolism ; Female ; Follicular Phase - physiology ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation, Enzymologic - physiology ; Gonadotropin-Releasing Hormone - pharmacology ; Gonadotropins - metabolism ; Hormone metabolism and regulation ; Mammalian female genital system ; Matrix Metalloproteinase 13 ; Ovarian Follicle - enzymology ; RNA, Messenger - metabolism ; Up-Regulation ; Vertebrates: reproduction</subject><ispartof>Biology of reproduction, 2004-08, Vol.71 (2), p.605-612</ispartof><rights>2004 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15969709$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15115716$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>BAKKE, Leanne J</creatorcontrib><creatorcontrib>QINGLEI LI</creatorcontrib><creatorcontrib>CASSAR, Carolyn A</creatorcontrib><creatorcontrib>DOW, Mark P. D</creatorcontrib><creatorcontrib>PURSLEY, J. Richard</creatorcontrib><creatorcontrib>SMITH, George W</creatorcontrib><title>Gonadotropin Surge-Induced Differential Upregulation of Collagenase-1 (MMP-1) and Collagenase-3 (MMP-13) mRNA and Protein in Bovine Preovulatory Follicles</title><title>Biology of reproduction</title><addtitle>Biol Reprod</addtitle><description>The ovulatory process is characterized by focalized extracellular matrix degradation at the apex of preovulatory follicles.
Many studies have implicated the matrix metalloproteinases (MMPs) as potential mediators of follicle rupture. Objectives of
this study were to determine localization and effect of the gonadotropin surge on temporal expression of MMP-1 and MMP-13
in bovine preovulatory follicles. Samples were collected at 0, 6, 12, 18, 24, and 48 h (corpora lutea) after GnRH injection
(n = 5â6 per time point) and amounts of MMP-1 and MMP-13 mRNA and protein determined using dot blot or semiquantitative RT-PCR
and Western blot analyses. Samples were also collected at 0 and 20 h after GnRH injection for immunohistochemical localization
of MMP-1 and MMP-13. Results indicate that follicular expression of MMP-1 and MMP-13 increased following the gonadotropin
surge. Abundance of MMP-1 mRNA increased at 6, 12, and 48 h post-GnRH injection. Immunoreactive MMP-1 was localized to granulosal
and thecal layers of preovulatory follicles. Amounts of MMP-1 protein increased in both the apex and the base of preovulatory
follicles. Abundance of MMP-13 mRNA increased at 6, 24, and 48 h post GnRH injection. Amounts of MMP-13 protein also increased
in the follicular apex and base. Immunoreactive MMP-13 was localized to granulosal and thecal layers of preovulatory follicles.
Results indicate MMP-1 and MMP-13 are increased in bovine preovulatory follicles following the gonadotropin surge but do not
support a requirement for differential up-regulation of MMP-1 and MMP-13 (follicular apex vs. base) for the preovulatory collagenolysis
required for follicle rupture.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cattle</subject><subject>Collagenases - genetics</subject><subject>Collagenases - metabolism</subject><subject>Female</subject><subject>Follicular Phase - physiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation, Enzymologic - physiology</subject><subject>Gonadotropin-Releasing Hormone - pharmacology</subject><subject>Gonadotropins - metabolism</subject><subject>Hormone metabolism and regulation</subject><subject>Mammalian female genital system</subject><subject>Matrix Metalloproteinase 13</subject><subject>Ovarian Follicle - enzymology</subject><subject>RNA, Messenger - metabolism</subject><subject>Up-Regulation</subject><subject>Vertebrates: reproduction</subject><issn>0006-3363</issn><issn>1529-7268</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkN1u1DAQhS1ERbeFRwD5BtRepPgntuPLstAfqYUK6HXkOJNdI8de7KSrvgpPi4GtCldIlkae880ZzUHoJSUnlGjxtnPRJ9ik2Jd_fUKYoo14ghZUMF0pJpunaEEIkRXnku-jg5y_EUJrzvgztE8FpUJRuUA_zmMwfZxS3LiAv8xpBdVl6GcLPX7vhgEShMkZj283CVazN5OLAccBL6P3ZgXBZKgoPrq-vqnoMTah_0fhO4Uf4_Hzx9Pf-k2KE5Rl5b2Ldy5A6UC8--Ud0z0-K-POesjP0d5gfIYXu3qIbs8-fF1eVFefzi-Xp1fVmulmqrgYBO0FMx2xSlnKOjbQTjBmJVVcCWWIaYZGGQ1c0q6rDYNOQgOs11aD4IfozR_fEub3GfLUji5bKEcEiHNupSxxylr-F6RKaSVYXcBXO3DuRujbTXKjSfftQ-wFeL0DTLbGD8kE6_JfnJZaEf3Ird1qvXUJ2jwa74stb7fbraItayUR_Cewi6DP</recordid><startdate>20040801</startdate><enddate>20040801</enddate><creator>BAKKE, Leanne J</creator><creator>QINGLEI LI</creator><creator>CASSAR, Carolyn A</creator><creator>DOW, Mark P. D</creator><creator>PURSLEY, J. Richard</creator><creator>SMITH, George W</creator><general>Society for the Study of Reproduction</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7TM</scope><scope>7X8</scope></search><sort><creationdate>20040801</creationdate><title>Gonadotropin Surge-Induced Differential Upregulation of Collagenase-1 (MMP-1) and Collagenase-3 (MMP-13) mRNA and Protein in Bovine Preovulatory Follicles</title><author>BAKKE, Leanne J ; QINGLEI LI ; CASSAR, Carolyn A ; DOW, Mark P. D ; PURSLEY, J. Richard ; SMITH, George W</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-h298t-35f51d52ab0c77c12b2f1b522c6173757a0a8f87a9e361bb4a2eb6e8e2d9c9e53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cattle</topic><topic>Collagenases - genetics</topic><topic>Collagenases - metabolism</topic><topic>Female</topic><topic>Follicular Phase - physiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Regulation, Enzymologic - physiology</topic><topic>Gonadotropin-Releasing Hormone - pharmacology</topic><topic>Gonadotropins - metabolism</topic><topic>Hormone metabolism and regulation</topic><topic>Mammalian female genital system</topic><topic>Matrix Metalloproteinase 13</topic><topic>Ovarian Follicle - enzymology</topic><topic>RNA, Messenger - metabolism</topic><topic>Up-Regulation</topic><topic>Vertebrates: reproduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>BAKKE, Leanne J</creatorcontrib><creatorcontrib>QINGLEI LI</creatorcontrib><creatorcontrib>CASSAR, Carolyn A</creatorcontrib><creatorcontrib>DOW, Mark P. D</creatorcontrib><creatorcontrib>PURSLEY, J. Richard</creatorcontrib><creatorcontrib>SMITH, George W</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Nucleic Acids Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biology of reproduction</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>BAKKE, Leanne J</au><au>QINGLEI LI</au><au>CASSAR, Carolyn A</au><au>DOW, Mark P. D</au><au>PURSLEY, J. Richard</au><au>SMITH, George W</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Gonadotropin Surge-Induced Differential Upregulation of Collagenase-1 (MMP-1) and Collagenase-3 (MMP-13) mRNA and Protein in Bovine Preovulatory Follicles</atitle><jtitle>Biology of reproduction</jtitle><addtitle>Biol Reprod</addtitle><date>2004-08-01</date><risdate>2004</risdate><volume>71</volume><issue>2</issue><spage>605</spage><epage>612</epage><pages>605-612</pages><issn>0006-3363</issn><eissn>1529-7268</eissn><coden>BIREBV</coden><abstract>The ovulatory process is characterized by focalized extracellular matrix degradation at the apex of preovulatory follicles.
Many studies have implicated the matrix metalloproteinases (MMPs) as potential mediators of follicle rupture. Objectives of
this study were to determine localization and effect of the gonadotropin surge on temporal expression of MMP-1 and MMP-13
in bovine preovulatory follicles. Samples were collected at 0, 6, 12, 18, 24, and 48 h (corpora lutea) after GnRH injection
(n = 5â6 per time point) and amounts of MMP-1 and MMP-13 mRNA and protein determined using dot blot or semiquantitative RT-PCR
and Western blot analyses. Samples were also collected at 0 and 20 h after GnRH injection for immunohistochemical localization
of MMP-1 and MMP-13. Results indicate that follicular expression of MMP-1 and MMP-13 increased following the gonadotropin
surge. Abundance of MMP-1 mRNA increased at 6, 12, and 48 h post-GnRH injection. Immunoreactive MMP-1 was localized to granulosal
and thecal layers of preovulatory follicles. Amounts of MMP-1 protein increased in both the apex and the base of preovulatory
follicles. Abundance of MMP-13 mRNA increased at 6, 24, and 48 h post GnRH injection. Amounts of MMP-13 protein also increased
in the follicular apex and base. Immunoreactive MMP-13 was localized to granulosal and thecal layers of preovulatory follicles.
Results indicate MMP-1 and MMP-13 are increased in bovine preovulatory follicles following the gonadotropin surge but do not
support a requirement for differential up-regulation of MMP-1 and MMP-13 (follicular apex vs. base) for the preovulatory collagenolysis
required for follicle rupture.</abstract><cop>Madison, WI</cop><pub>Society for the Study of Reproduction</pub><pmid>15115716</pmid><doi>10.1095/biolreprod.104.027185</doi><tpages>8</tpages></addata></record> |
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| ispartof | Biology of reproduction, 2004-08, Vol.71 (2), p.605-612 |
| issn | 0006-3363 1529-7268 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_66726646 |
| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE; EZB-FREE-00999 freely available EZB journals; BioOne Complete |
| subjects | Animals Biological and medical sciences Cattle Collagenases - genetics Collagenases - metabolism Female Follicular Phase - physiology Fundamental and applied biological sciences. Psychology Gene Expression Regulation, Enzymologic - physiology Gonadotropin-Releasing Hormone - pharmacology Gonadotropins - metabolism Hormone metabolism and regulation Mammalian female genital system Matrix Metalloproteinase 13 Ovarian Follicle - enzymology RNA, Messenger - metabolism Up-Regulation Vertebrates: reproduction |
| title | Gonadotropin Surge-Induced Differential Upregulation of Collagenase-1 (MMP-1) and Collagenase-3 (MMP-13) mRNA and Protein in Bovine Preovulatory Follicles |
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