Nitrogen disruption of synaptoneurosomes: an alternative method to isolate brain mitochondria

Mitochondria are known to be localized in synaptic and non-synaptic compartments in the brain. Synaptoneurosomes, which contain high numbers of mitochondria, may act as a major contaminant of currently used isolation techniques. Currently, there is no method employed to successfully disrupt synapton...

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Veröffentlicht in:	Journal of neuroscience methods 2004-08, Vol.137 (2), p.299-303
Hauptverfasser:	Brown, Maile R, Sullivan, Patrick G, Dorenbos, Kristina A, Modafferi, Edward A, Geddes, James W, Steward, Oswald
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Blotting, Western - methods Brain - cytology Cytological Techniques - methods Digitonin - pharmacology Electron Transport Complex IV - metabolism Isolation brain mitochondria Male Microscopy, Electron - methods Mitochondria Mitochondria - drug effects Mitochondria - metabolism Mitochondria - ultrastructure Nerve Tissue Proteins - metabolism Nitrogen - pharmacology Nitrogen decompression Non-synaptic Oxygen Consumption Porins Rats Subcellular Fractions Synaptic Synaptosomes - drug effects Total Voltage-Dependent Anion Channels
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