Gene expression in juvenile arthritis and spondyloarthropathy: pro-angiogenic ELR+ chemokine genes relate to course of arthritis
Objective. To evaluate the ability of microarray-based methods to identify genes with disease-specific expression patterns in peripheral blood mononuclear cells (PBMC) and synovial fluid mononuclear cells (SFMC) of juvenile arthritis patients and healthy controls. Methods. Microarray data (Affymetri...
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| Veröffentlicht in: | Rheumatology (Oxford, England) England), 2004-08, Vol.43 (8), p.973-979 |
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| creator | Barnes, M. G. Aronow, B. J. Luyrink, L. K. Moroldo, M. B. Pavlidis, P. Passo, M. H. Grom, A. A. Hirsch, R. Giannini, E. H. Colbert, R. A. Glass, D. N. Thompson, S. D. |
| description | Objective. To evaluate the ability of microarray-based methods to identify genes with disease-specific expression patterns in peripheral blood mononuclear cells (PBMC) and synovial fluid mononuclear cells (SFMC) of juvenile arthritis patients and healthy controls. Methods. Microarray data (Affymetrix U95Av2) from 26 PBMC and 20 SFMC samples collected from patients with active disease (classified by course according to ACR criteria) were analysed for expression patterns that correlated with disease characteristics. For comparison, PBMC gene expression profiles were obtained from 15 healthy controls. Real-time PCR was used for confirmation of gene expression differences. Results. Statistical analysis of gene expression patterns in PBMC identified 378 probe sets corresponding to 342 unique genes with differing expression levels between polyarticular course patients and controls (t test, P |
| doi_str_mv | 10.1093/rheumatology/keh224 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_66723739</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>66723739</sourcerecordid><originalsourceid>FETCH-LOGICAL-c538t-6138484fe51201121aa0890ce94750f15a568cb4558127040fd3be311cec1fa83</originalsourceid><addsrcrecordid>eNqFkU1v1DAQhiMEoqXwC5CQhQQXFOrxR-L0hqrSIlbiGyEultc72fVuYgc7Qd0bPx2XXXURF062Zp555x29RfEY6EugDT-NK5x6M4YuLLenG1wxJu4UxyAqVlLO2d3bPxNHxYOU1pRSCVzdL45AgqSC8-Pi1yV6JHg9REzJBU-cJ-vpJ3rXITFxXEU3ukSMX5A0BL_YduFPNQxmXG3PyBBDafzShWUeseRi9vEFsSvsw8Zl3VzERCJ2ZkQyBmLDFBOS0B6kHxb3WtMlfLR_T4ovry8-n1-Vs3eXb85fzUoruRrLKhsXSrQogVEABsZQ1VCLjaglbUEaWSk7F1IqYDUVtF3wOXIAixZao_hJ8Xynmx3_mDCNunfJYtcZj2FKuqpqxmve_BeERtUKKpHBp_-A63yez0dkJrtRvLlZy3eQjSGliK0eoutN3Gqg-iZG_XeMehdjnnqyl57mPS4OM_vcMvBsD5hkTddG461LB66iUNWcZa7ccS6NeH3bN3Gjc7-W-urbdw3w4f3bT1-5Vvw3xES6nQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>195688398</pqid></control><display><type>article</type><title>Gene expression in juvenile arthritis and spondyloarthropathy: pro-angiogenic ELR+ chemokine genes relate to course of arthritis</title><source>Oxford University Press Journals All Titles (1996-Current)</source><source>MEDLINE</source><source>Alma/SFX Local Collection</source><creator>Barnes, M. G. ; Aronow, B. J. ; Luyrink, L. K. ; Moroldo, M. B. ; Pavlidis, P. ; Passo, M. H. ; Grom, A. A. ; Hirsch, R. ; Giannini, E. H. ; Colbert, R. A. ; Glass, D. N. ; Thompson, S. D.</creator><creatorcontrib>Barnes, M. G. ; Aronow, B. J. ; Luyrink, L. K. ; Moroldo, M. B. ; Pavlidis, P. ; Passo, M. H. ; Grom, A. A. ; Hirsch, R. ; Giannini, E. H. ; Colbert, R. A. ; Glass, D. N. ; Thompson, S. D.</creatorcontrib><description>Objective. To evaluate the ability of microarray-based methods to identify genes with disease-specific expression patterns in peripheral blood mononuclear cells (PBMC) and synovial fluid mononuclear cells (SFMC) of juvenile arthritis patients and healthy controls. Methods. Microarray data (Affymetrix U95Av2) from 26 PBMC and 20 SFMC samples collected from patients with active disease (classified by course according to ACR criteria) were analysed for expression patterns that correlated with disease characteristics. For comparison, PBMC gene expression profiles were obtained from 15 healthy controls. Real-time PCR was used for confirmation of gene expression differences. Results. Statistical analysis of gene expression patterns in PBMC identified 378 probe sets corresponding to 342 unique genes with differing expression levels between polyarticular course patients and controls (t test, P<0.0001). The genes represented by these probe sets were enriched for functions related to regulation of immune cell functions, receptor signalling as well as protein metabolism and degradation. Included in these probe sets were a group of CXCL chemokines with functions related to angiogenesis. Further analysis showed that, whereas angiogenic CXCL (ELR+) gene expression was elevated in polyarticular PBMC, expression of angiostatic CXCL (ELR−) chemokines was lower in polyarticular SFMC compared with corresponding pauciarticular samples (t test, P<0.05). Conclusions. This pilot study demonstrates that juvenile arthritis patients exhibit complex patterns of gene expression in PBMC and SFMC. The presence of disease-correlated biologically relevant gene expression patterns suggests that the power of this approach will allow better understanding of disease mechanisms, identify distinct clinical phenotypes in disease subtypes, and suggest new therapeutic approaches.</description><identifier>ISSN: 1462-0324</identifier><identifier>EISSN: 1462-0332</identifier><identifier>DOI: 10.1093/rheumatology/keh224</identifier><identifier>PMID: 15150433</identifier><identifier>CODEN: BJRHDF</identifier><language>eng</language><publisher>Oxford: Oxford University Press</publisher><subject>Adolescent ; Adult ; Arthritis, Juvenile - genetics ; Biological and medical sciences ; Cells, Cultured ; Chemokines ; Chemokines, CXC - genetics ; Child ; Diseases of the osteoarticular system ; Gene Expression - genetics ; Gene Expression Profiling - methods ; Human ; Humans ; Inflammation ; Inflammatory joint diseases ; JAK/STAT ; Juvenile ; Leukocytes, Mononuclear - physiology ; Medical sciences ; Miscellaneous. Osteoarticular involvement in other diseases ; Neovascularization, Pathologic - genetics ; Oligonucleotide Array Sequence Analysis - methods ; Pilot Projects ; Polyarticular course ; Protein-Tyrosine Kinases - genetics ; Retrospective Studies ; Rheumatoid arthritis ; Signal Transduction - genetics ; Spondylarthropathies - genetics ; Synovial Fluid - physiology ; Trans-Activators - genetics</subject><ispartof>Rheumatology (Oxford, England), 2004-08, Vol.43 (8), p.973-979</ispartof><rights>2004 INIST-CNRS</rights><rights>Copyright Oxford University Press(England) Aug 2004</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c538t-6138484fe51201121aa0890ce94750f15a568cb4558127040fd3be311cec1fa83</citedby><cites>FETCH-LOGICAL-c538t-6138484fe51201121aa0890ce94750f15a568cb4558127040fd3be311cec1fa83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16016732$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15150433$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Barnes, M. G.</creatorcontrib><creatorcontrib>Aronow, B. J.</creatorcontrib><creatorcontrib>Luyrink, L. K.</creatorcontrib><creatorcontrib>Moroldo, M. B.</creatorcontrib><creatorcontrib>Pavlidis, P.</creatorcontrib><creatorcontrib>Passo, M. H.</creatorcontrib><creatorcontrib>Grom, A. A.</creatorcontrib><creatorcontrib>Hirsch, R.</creatorcontrib><creatorcontrib>Giannini, E. H.</creatorcontrib><creatorcontrib>Colbert, R. A.</creatorcontrib><creatorcontrib>Glass, D. N.</creatorcontrib><creatorcontrib>Thompson, S. D.</creatorcontrib><title>Gene expression in juvenile arthritis and spondyloarthropathy: pro-angiogenic ELR+ chemokine genes relate to course of arthritis</title><title>Rheumatology (Oxford, England)</title><addtitle>Rheumatology</addtitle><description>Objective. To evaluate the ability of microarray-based methods to identify genes with disease-specific expression patterns in peripheral blood mononuclear cells (PBMC) and synovial fluid mononuclear cells (SFMC) of juvenile arthritis patients and healthy controls. Methods. Microarray data (Affymetrix U95Av2) from 26 PBMC and 20 SFMC samples collected from patients with active disease (classified by course according to ACR criteria) were analysed for expression patterns that correlated with disease characteristics. For comparison, PBMC gene expression profiles were obtained from 15 healthy controls. Real-time PCR was used for confirmation of gene expression differences. Results. Statistical analysis of gene expression patterns in PBMC identified 378 probe sets corresponding to 342 unique genes with differing expression levels between polyarticular course patients and controls (t test, P<0.0001). The genes represented by these probe sets were enriched for functions related to regulation of immune cell functions, receptor signalling as well as protein metabolism and degradation. Included in these probe sets were a group of CXCL chemokines with functions related to angiogenesis. Further analysis showed that, whereas angiogenic CXCL (ELR+) gene expression was elevated in polyarticular PBMC, expression of angiostatic CXCL (ELR−) chemokines was lower in polyarticular SFMC compared with corresponding pauciarticular samples (t test, P<0.05). Conclusions. This pilot study demonstrates that juvenile arthritis patients exhibit complex patterns of gene expression in PBMC and SFMC. The presence of disease-correlated biologically relevant gene expression patterns suggests that the power of this approach will allow better understanding of disease mechanisms, identify distinct clinical phenotypes in disease subtypes, and suggest new therapeutic approaches.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Arthritis, Juvenile - genetics</subject><subject>Biological and medical sciences</subject><subject>Cells, Cultured</subject><subject>Chemokines</subject><subject>Chemokines, CXC - genetics</subject><subject>Child</subject><subject>Diseases of the osteoarticular system</subject><subject>Gene Expression - genetics</subject><subject>Gene Expression Profiling - methods</subject><subject>Human</subject><subject>Humans</subject><subject>Inflammation</subject><subject>Inflammatory joint diseases</subject><subject>JAK/STAT</subject><subject>Juvenile</subject><subject>Leukocytes, Mononuclear - physiology</subject><subject>Medical sciences</subject><subject>Miscellaneous. Osteoarticular involvement in other diseases</subject><subject>Neovascularization, Pathologic - genetics</subject><subject>Oligonucleotide Array Sequence Analysis - methods</subject><subject>Pilot Projects</subject><subject>Polyarticular course</subject><subject>Protein-Tyrosine Kinases - genetics</subject><subject>Retrospective Studies</subject><subject>Rheumatoid arthritis</subject><subject>Signal Transduction - genetics</subject><subject>Spondylarthropathies - genetics</subject><subject>Synovial Fluid - physiology</subject><subject>Trans-Activators - genetics</subject><issn>1462-0324</issn><issn>1462-0332</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1v1DAQhiMEoqXwC5CQhQQXFOrxR-L0hqrSIlbiGyEultc72fVuYgc7Qd0bPx2XXXURF062Zp555x29RfEY6EugDT-NK5x6M4YuLLenG1wxJu4UxyAqVlLO2d3bPxNHxYOU1pRSCVzdL45AgqSC8-Pi1yV6JHg9REzJBU-cJ-vpJ3rXITFxXEU3ukSMX5A0BL_YduFPNQxmXG3PyBBDafzShWUeseRi9vEFsSvsw8Zl3VzERCJ2ZkQyBmLDFBOS0B6kHxb3WtMlfLR_T4ovry8-n1-Vs3eXb85fzUoruRrLKhsXSrQogVEABsZQ1VCLjaglbUEaWSk7F1IqYDUVtF3wOXIAixZao_hJ8Xynmx3_mDCNunfJYtcZj2FKuqpqxmve_BeERtUKKpHBp_-A63yez0dkJrtRvLlZy3eQjSGliK0eoutN3Gqg-iZG_XeMehdjnnqyl57mPS4OM_vcMvBsD5hkTddG461LB66iUNWcZa7ccS6NeH3bN3Gjc7-W-urbdw3w4f3bT1-5Vvw3xES6nQ</recordid><startdate>20040801</startdate><enddate>20040801</enddate><creator>Barnes, M. G.</creator><creator>Aronow, B. J.</creator><creator>Luyrink, L. K.</creator><creator>Moroldo, M. B.</creator><creator>Pavlidis, P.</creator><creator>Passo, M. H.</creator><creator>Grom, A. A.</creator><creator>Hirsch, R.</creator><creator>Giannini, E. H.</creator><creator>Colbert, R. A.</creator><creator>Glass, D. N.</creator><creator>Thompson, S. D.</creator><general>Oxford University Press</general><general>Oxford Publishing Limited (England)</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>K9.</scope><scope>NAPCQ</scope><scope>7T5</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20040801</creationdate><title>Gene expression in juvenile arthritis and spondyloarthropathy: pro-angiogenic ELR+ chemokine genes relate to course of arthritis</title><author>Barnes, M. G. ; Aronow, B. J. ; Luyrink, L. K. ; Moroldo, M. B. ; Pavlidis, P. ; Passo, M. H. ; Grom, A. A. ; Hirsch, R. ; Giannini, E. H. ; Colbert, R. A. ; Glass, D. N. ; Thompson, S. D.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c538t-6138484fe51201121aa0890ce94750f15a568cb4558127040fd3be311cec1fa83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Arthritis, Juvenile - genetics</topic><topic>Biological and medical sciences</topic><topic>Cells, Cultured</topic><topic>Chemokines</topic><topic>Chemokines, CXC - genetics</topic><topic>Child</topic><topic>Diseases of the osteoarticular system</topic><topic>Gene Expression - genetics</topic><topic>Gene Expression Profiling - methods</topic><topic>Human</topic><topic>Humans</topic><topic>Inflammation</topic><topic>Inflammatory joint diseases</topic><topic>JAK/STAT</topic><topic>Juvenile</topic><topic>Leukocytes, Mononuclear - physiology</topic><topic>Medical sciences</topic><topic>Miscellaneous. Osteoarticular involvement in other diseases</topic><topic>Neovascularization, Pathologic - genetics</topic><topic>Oligonucleotide Array Sequence Analysis - methods</topic><topic>Pilot Projects</topic><topic>Polyarticular course</topic><topic>Protein-Tyrosine Kinases - genetics</topic><topic>Retrospective Studies</topic><topic>Rheumatoid arthritis</topic><topic>Signal Transduction - genetics</topic><topic>Spondylarthropathies - genetics</topic><topic>Synovial Fluid - physiology</topic><topic>Trans-Activators - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Barnes, M. G.</creatorcontrib><creatorcontrib>Aronow, B. J.</creatorcontrib><creatorcontrib>Luyrink, L. K.</creatorcontrib><creatorcontrib>Moroldo, M. B.</creatorcontrib><creatorcontrib>Pavlidis, P.</creatorcontrib><creatorcontrib>Passo, M. H.</creatorcontrib><creatorcontrib>Grom, A. A.</creatorcontrib><creatorcontrib>Hirsch, R.</creatorcontrib><creatorcontrib>Giannini, E. H.</creatorcontrib><creatorcontrib>Colbert, R. A.</creatorcontrib><creatorcontrib>Glass, D. N.</creatorcontrib><creatorcontrib>Thompson, S. D.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Premium</collection><collection>Immunology Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Rheumatology (Oxford, England)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Barnes, M. G.</au><au>Aronow, B. J.</au><au>Luyrink, L. K.</au><au>Moroldo, M. B.</au><au>Pavlidis, P.</au><au>Passo, M. H.</au><au>Grom, A. A.</au><au>Hirsch, R.</au><au>Giannini, E. H.</au><au>Colbert, R. A.</au><au>Glass, D. N.</au><au>Thompson, S. D.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Gene expression in juvenile arthritis and spondyloarthropathy: pro-angiogenic ELR+ chemokine genes relate to course of arthritis</atitle><jtitle>Rheumatology (Oxford, England)</jtitle><addtitle>Rheumatology</addtitle><date>2004-08-01</date><risdate>2004</risdate><volume>43</volume><issue>8</issue><spage>973</spage><epage>979</epage><pages>973-979</pages><issn>1462-0324</issn><eissn>1462-0332</eissn><coden>BJRHDF</coden><abstract>Objective. To evaluate the ability of microarray-based methods to identify genes with disease-specific expression patterns in peripheral blood mononuclear cells (PBMC) and synovial fluid mononuclear cells (SFMC) of juvenile arthritis patients and healthy controls. Methods. Microarray data (Affymetrix U95Av2) from 26 PBMC and 20 SFMC samples collected from patients with active disease (classified by course according to ACR criteria) were analysed for expression patterns that correlated with disease characteristics. For comparison, PBMC gene expression profiles were obtained from 15 healthy controls. Real-time PCR was used for confirmation of gene expression differences. Results. Statistical analysis of gene expression patterns in PBMC identified 378 probe sets corresponding to 342 unique genes with differing expression levels between polyarticular course patients and controls (t test, P<0.0001). The genes represented by these probe sets were enriched for functions related to regulation of immune cell functions, receptor signalling as well as protein metabolism and degradation. Included in these probe sets were a group of CXCL chemokines with functions related to angiogenesis. Further analysis showed that, whereas angiogenic CXCL (ELR+) gene expression was elevated in polyarticular PBMC, expression of angiostatic CXCL (ELR−) chemokines was lower in polyarticular SFMC compared with corresponding pauciarticular samples (t test, P<0.05). Conclusions. This pilot study demonstrates that juvenile arthritis patients exhibit complex patterns of gene expression in PBMC and SFMC. The presence of disease-correlated biologically relevant gene expression patterns suggests that the power of this approach will allow better understanding of disease mechanisms, identify distinct clinical phenotypes in disease subtypes, and suggest new therapeutic approaches.</abstract><cop>Oxford</cop><pub>Oxford University Press</pub><pmid>15150433</pmid><doi>10.1093/rheumatology/keh224</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE; Alma/SFX Local Collection |
| subjects | Adolescent Adult Arthritis, Juvenile - genetics Biological and medical sciences Cells, Cultured Chemokines Chemokines, CXC - genetics Child Diseases of the osteoarticular system Gene Expression - genetics Gene Expression Profiling - methods Human Humans Inflammation Inflammatory joint diseases JAK/STAT Juvenile Leukocytes, Mononuclear - physiology Medical sciences Miscellaneous. Osteoarticular involvement in other diseases Neovascularization, Pathologic - genetics Oligonucleotide Array Sequence Analysis - methods Pilot Projects Polyarticular course Protein-Tyrosine Kinases - genetics Retrospective Studies Rheumatoid arthritis Signal Transduction - genetics Spondylarthropathies - genetics Synovial Fluid - physiology Trans-Activators - genetics |
| title | Gene expression in juvenile arthritis and spondyloarthropathy: pro-angiogenic ELR+ chemokine genes relate to course of arthritis |
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