CDK9/CYCLIN T1 expression during normal lymphoid differentiation and malignant transformation
CDK9 is a member of the CDC2‐like family of kinases. Its cyclin partners are members of the CYCLIN T family (T1, T2a, and T2b) and CYCLIN K. The CDK9/CYCLIN T1 complex is very important in the differentiation programme of several cell types, controlling specific differentiation pathways. Limited dat...
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| creator | Bellan, Cristiana De Falco, Giulia Lazzi, Stefano Micheli, Pietro Vicidomini, Sonia Schürfeld, Karin Amato, Teresa Palumbo, Annalisa Bagella, Luigi Sabattini, Elena Bartolommei, Sabrina Hummel, Michael Pileri, Stefano Tosi, Piero Leoncini, Lorenzo Giordano, Antonio |
| description | CDK9 is a member of the CDC2‐like family of kinases. Its cyclin partners are members of the CYCLIN T family (T1, T2a, and T2b) and CYCLIN K. The CDK9/CYCLIN T1 complex is very important in the differentiation programme of several cell types, controlling specific differentiation pathways. Limited data are available regarding the expression of CDK9/CYCLIN T1 in haematopoietic and lymphoid tissues. The aim of this study was to analyse the expression of the CDK9/CYCLIN T1 complex in lymphoid tissue, in order to assess its role in B‐ and T‐cell differentiation and lymphomagenesis. CDK9/CYCLIN T1 expression was found by immunohistochemistry in precursor B and T cells. In peripheral lymphoid tissues, germinal centre cells and scattered B‐ and T‐cell blasts in interfollicular areas expressed CDK9/CYCLIN T1, while mantle cells, plasma cells, and small resting T‐lymphocytes displayed no expression of either molecule. CDK9/CYCLIN T1 expression therefore appears to be related to particular stages of lymphoid differentiation/activation. CDK9 and CYCLIN T1 were highly expressed in lymphomas derived from precursor B and T cells, from germinal centre cells, such as follicular lymphomas, and from activated T cells (ie anaplastic large cell lymphomas). Hodgkin and Reed–Sternberg cells of classical Hodgkin's lymphoma also showed strong nuclear staining. Diffuse large B‐cell, Burkitt's lymphomas, and peripheral T‐cell lymphomas, among T‐cell lymphoproliferative disorders, showed a wide range of values. No expression of CDK9 or CYCLIN T1 was detected in mantle cell and marginal zone lymphomas. However, at the mRNA level, an imbalance in the CDK9/CYCLIN T1 ratio was found in follicular lymphoma and diffuse large B‐cell lymphomas with germinal centre phenotype, and in the cell lines of classical Hodgkin's lymphomas, Burkitt's lymphomas, and anaplastic large cell lymphoma, in comparison with reactive lymph nodes. These results suggest that the CDK9/CYCLIN T1 complex may affect the activation and differentiation programme of lymphoid cells. The molecular mechanism through which the CDK9/CYCLIN T1 complex is altered in malignant transformation needs to be elucidated. Copyright © 2004 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd. |
| doi_str_mv | 10.1002/path.1588 |
| format | Article |
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Its cyclin partners are members of the CYCLIN T family (T1, T2a, and T2b) and CYCLIN K. The CDK9/CYCLIN T1 complex is very important in the differentiation programme of several cell types, controlling specific differentiation pathways. Limited data are available regarding the expression of CDK9/CYCLIN T1 in haematopoietic and lymphoid tissues. The aim of this study was to analyse the expression of the CDK9/CYCLIN T1 complex in lymphoid tissue, in order to assess its role in B‐ and T‐cell differentiation and lymphomagenesis. CDK9/CYCLIN T1 expression was found by immunohistochemistry in precursor B and T cells. In peripheral lymphoid tissues, germinal centre cells and scattered B‐ and T‐cell blasts in interfollicular areas expressed CDK9/CYCLIN T1, while mantle cells, plasma cells, and small resting T‐lymphocytes displayed no expression of either molecule. CDK9/CYCLIN T1 expression therefore appears to be related to particular stages of lymphoid differentiation/activation. CDK9 and CYCLIN T1 were highly expressed in lymphomas derived from precursor B and T cells, from germinal centre cells, such as follicular lymphomas, and from activated T cells (ie anaplastic large cell lymphomas). Hodgkin and Reed–Sternberg cells of classical Hodgkin's lymphoma also showed strong nuclear staining. Diffuse large B‐cell, Burkitt's lymphomas, and peripheral T‐cell lymphomas, among T‐cell lymphoproliferative disorders, showed a wide range of values. No expression of CDK9 or CYCLIN T1 was detected in mantle cell and marginal zone lymphomas. However, at the mRNA level, an imbalance in the CDK9/CYCLIN T1 ratio was found in follicular lymphoma and diffuse large B‐cell lymphomas with germinal centre phenotype, and in the cell lines of classical Hodgkin's lymphomas, Burkitt's lymphomas, and anaplastic large cell lymphoma, in comparison with reactive lymph nodes. These results suggest that the CDK9/CYCLIN T1 complex may affect the activation and differentiation programme of lymphoid cells. The molecular mechanism through which the CDK9/CYCLIN T1 complex is altered in malignant transformation needs to be elucidated. Copyright © 2004 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.</description><identifier>ISSN: 0022-3417</identifier><identifier>EISSN: 1096-9896</identifier><identifier>DOI: 10.1002/path.1588</identifier><identifier>PMID: 15258998</identifier><identifier>CODEN: JPTLAS</identifier><language>eng</language><publisher>Chichester, UK: John Wiley & Sons, Ltd</publisher><subject>Biological and medical sciences ; Blotting, Western ; CDK9 ; Cell Differentiation ; Cell Transformation, Neoplastic - metabolism ; Cell Transformation, Neoplastic - pathology ; CYCLIN T ; Cyclin-Dependent Kinase 9 - genetics ; Cyclin-Dependent Kinase 9 - metabolism ; Cyclins - genetics ; Cyclins - metabolism ; differentiation ; Female ; Gene Expression ; Hematologic and hematopoietic diseases ; Humans ; Immunoenzyme Techniques ; Investigative techniques, diagnostic techniques (general aspects) ; Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis ; lymphadenitis ; lymphocyte ; lymphoma ; Lymphoma, B-Cell - metabolism ; Lymphoma, B-Cell - pathology ; Lymphoma, T-Cell - metabolism ; Lymphoma, T-Cell - pathology ; Male ; Medical sciences ; Neoplasm Proteins - genetics ; Neoplasm Proteins - metabolism ; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Messenger - genetics</subject><ispartof>The Journal of pathology, 2004-08, Vol.203 (4), p.946-952</ispartof><rights>Copyright © 2004 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.</rights><rights>2004 INIST-CNRS</rights><rights>Copyright 2004 Pathological Society of Great Britain and Ireland</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5198-613e966fd83c555cfdae5bf0402d5c74dec1c5a9ba0115a33a33020fd143b743</citedby><cites>FETCH-LOGICAL-c5198-613e966fd83c555cfdae5bf0402d5c74dec1c5a9ba0115a33a33020fd143b743</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fpath.1588$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fpath.1588$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15954956$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15258998$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bellan, Cristiana</creatorcontrib><creatorcontrib>De Falco, Giulia</creatorcontrib><creatorcontrib>Lazzi, Stefano</creatorcontrib><creatorcontrib>Micheli, Pietro</creatorcontrib><creatorcontrib>Vicidomini, Sonia</creatorcontrib><creatorcontrib>Schürfeld, Karin</creatorcontrib><creatorcontrib>Amato, Teresa</creatorcontrib><creatorcontrib>Palumbo, Annalisa</creatorcontrib><creatorcontrib>Bagella, Luigi</creatorcontrib><creatorcontrib>Sabattini, Elena</creatorcontrib><creatorcontrib>Bartolommei, Sabrina</creatorcontrib><creatorcontrib>Hummel, Michael</creatorcontrib><creatorcontrib>Pileri, Stefano</creatorcontrib><creatorcontrib>Tosi, Piero</creatorcontrib><creatorcontrib>Leoncini, Lorenzo</creatorcontrib><creatorcontrib>Giordano, Antonio</creatorcontrib><title>CDK9/CYCLIN T1 expression during normal lymphoid differentiation and malignant transformation</title><title>The Journal of pathology</title><addtitle>J. Pathol</addtitle><description>CDK9 is a member of the CDC2‐like family of kinases. Its cyclin partners are members of the CYCLIN T family (T1, T2a, and T2b) and CYCLIN K. The CDK9/CYCLIN T1 complex is very important in the differentiation programme of several cell types, controlling specific differentiation pathways. Limited data are available regarding the expression of CDK9/CYCLIN T1 in haematopoietic and lymphoid tissues. The aim of this study was to analyse the expression of the CDK9/CYCLIN T1 complex in lymphoid tissue, in order to assess its role in B‐ and T‐cell differentiation and lymphomagenesis. CDK9/CYCLIN T1 expression was found by immunohistochemistry in precursor B and T cells. In peripheral lymphoid tissues, germinal centre cells and scattered B‐ and T‐cell blasts in interfollicular areas expressed CDK9/CYCLIN T1, while mantle cells, plasma cells, and small resting T‐lymphocytes displayed no expression of either molecule. CDK9/CYCLIN T1 expression therefore appears to be related to particular stages of lymphoid differentiation/activation. CDK9 and CYCLIN T1 were highly expressed in lymphomas derived from precursor B and T cells, from germinal centre cells, such as follicular lymphomas, and from activated T cells (ie anaplastic large cell lymphomas). Hodgkin and Reed–Sternberg cells of classical Hodgkin's lymphoma also showed strong nuclear staining. Diffuse large B‐cell, Burkitt's lymphomas, and peripheral T‐cell lymphomas, among T‐cell lymphoproliferative disorders, showed a wide range of values. No expression of CDK9 or CYCLIN T1 was detected in mantle cell and marginal zone lymphomas. However, at the mRNA level, an imbalance in the CDK9/CYCLIN T1 ratio was found in follicular lymphoma and diffuse large B‐cell lymphomas with germinal centre phenotype, and in the cell lines of classical Hodgkin's lymphomas, Burkitt's lymphomas, and anaplastic large cell lymphoma, in comparison with reactive lymph nodes. These results suggest that the CDK9/CYCLIN T1 complex may affect the activation and differentiation programme of lymphoid cells. The molecular mechanism through which the CDK9/CYCLIN T1 complex is altered in malignant transformation needs to be elucidated. Copyright © 2004 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.</description><subject>Biological and medical sciences</subject><subject>Blotting, Western</subject><subject>CDK9</subject><subject>Cell Differentiation</subject><subject>Cell Transformation, Neoplastic - metabolism</subject><subject>Cell Transformation, Neoplastic - pathology</subject><subject>CYCLIN T</subject><subject>Cyclin-Dependent Kinase 9 - genetics</subject><subject>Cyclin-Dependent Kinase 9 - metabolism</subject><subject>Cyclins - genetics</subject><subject>Cyclins - metabolism</subject><subject>differentiation</subject><subject>Female</subject><subject>Gene Expression</subject><subject>Hematologic and hematopoietic diseases</subject><subject>Humans</subject><subject>Immunoenzyme Techniques</subject><subject>Investigative techniques, diagnostic techniques (general aspects)</subject><subject>Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis</subject><subject>lymphadenitis</subject><subject>lymphocyte</subject><subject>lymphoma</subject><subject>Lymphoma, B-Cell - metabolism</subject><subject>Lymphoma, B-Cell - pathology</subject><subject>Lymphoma, T-Cell - metabolism</subject><subject>Lymphoma, T-Cell - pathology</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Neoplasm Proteins - genetics</subject><subject>Neoplasm Proteins - metabolism</subject><subject>Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Messenger - genetics</subject><issn>0022-3417</issn><issn>1096-9896</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0U1v1DAQBmALgei2cOAPoFxA5ZCunWT8cawCdKuuFoRWIA7I8vqjNSROsLOi--9JtBFwASRLPswzM9K8CD0j-IJgXCx7NdxdEOD8AVoQLGguuKAP0WKsFXlZEXaCTlP6ijEWAuAxOiFQABeCL9CX-vWNWNaf6_X1JtuSzN730abku5CZffThNgtdbFWTNYe2v-u8yYx3zkYbBq-GialgshH426DCkA1RheSmlqn4BD1yqkn26fyfoe3bN9t6la_fXV3Xl-tcAxE8p6S0glJneKkBQDujLOwcrnBhQLPKWE00KLFTmBBQZTk-XGBnSFXuWFWeoZfHsX3svu9tGmTrk7ZNo4Lt9klSyoqCCjbC839CwhlwwEz8fyZhlIuCwwhfHaGOXUrROtlH36p4kATLKR45xSOneEb7fB6637XW_JZzHiN4MQOVtGrceE3t0x9OQCWAjm55dD98Yw9_3yjfX25X8-r82OHTYO9_daj4TVJWMpCfNlfyo7j5sNpQLNflT5mXtgM</recordid><startdate>200408</startdate><enddate>200408</enddate><creator>Bellan, Cristiana</creator><creator>De Falco, Giulia</creator><creator>Lazzi, Stefano</creator><creator>Micheli, Pietro</creator><creator>Vicidomini, Sonia</creator><creator>Schürfeld, Karin</creator><creator>Amato, Teresa</creator><creator>Palumbo, Annalisa</creator><creator>Bagella, Luigi</creator><creator>Sabattini, Elena</creator><creator>Bartolommei, Sabrina</creator><creator>Hummel, Michael</creator><creator>Pileri, Stefano</creator><creator>Tosi, Piero</creator><creator>Leoncini, Lorenzo</creator><creator>Giordano, Antonio</creator><general>John Wiley & Sons, Ltd</general><general>Wiley</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TO</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>200408</creationdate><title>CDK9/CYCLIN T1 expression during normal lymphoid differentiation and malignant transformation</title><author>Bellan, Cristiana ; De Falco, Giulia ; Lazzi, Stefano ; Micheli, Pietro ; Vicidomini, Sonia ; Schürfeld, Karin ; Amato, Teresa ; Palumbo, Annalisa ; Bagella, Luigi ; Sabattini, Elena ; Bartolommei, Sabrina ; Hummel, Michael ; Pileri, Stefano ; Tosi, Piero ; Leoncini, Lorenzo ; Giordano, Antonio</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5198-613e966fd83c555cfdae5bf0402d5c74dec1c5a9ba0115a33a33020fd143b743</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Biological and medical sciences</topic><topic>Blotting, Western</topic><topic>CDK9</topic><topic>Cell Differentiation</topic><topic>Cell Transformation, Neoplastic - metabolism</topic><topic>Cell Transformation, Neoplastic - pathology</topic><topic>CYCLIN T</topic><topic>Cyclin-Dependent Kinase 9 - genetics</topic><topic>Cyclin-Dependent Kinase 9 - metabolism</topic><topic>Cyclins - genetics</topic><topic>Cyclins - metabolism</topic><topic>differentiation</topic><topic>Female</topic><topic>Gene Expression</topic><topic>Hematologic and hematopoietic diseases</topic><topic>Humans</topic><topic>Immunoenzyme Techniques</topic><topic>Investigative techniques, diagnostic techniques (general aspects)</topic><topic>Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis</topic><topic>lymphadenitis</topic><topic>lymphocyte</topic><topic>lymphoma</topic><topic>Lymphoma, B-Cell - metabolism</topic><topic>Lymphoma, B-Cell - pathology</topic><topic>Lymphoma, T-Cell - metabolism</topic><topic>Lymphoma, T-Cell - pathology</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Neoplasm Proteins - genetics</topic><topic>Neoplasm Proteins - metabolism</topic><topic>Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Messenger - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bellan, Cristiana</creatorcontrib><creatorcontrib>De Falco, Giulia</creatorcontrib><creatorcontrib>Lazzi, Stefano</creatorcontrib><creatorcontrib>Micheli, Pietro</creatorcontrib><creatorcontrib>Vicidomini, Sonia</creatorcontrib><creatorcontrib>Schürfeld, Karin</creatorcontrib><creatorcontrib>Amato, Teresa</creatorcontrib><creatorcontrib>Palumbo, Annalisa</creatorcontrib><creatorcontrib>Bagella, Luigi</creatorcontrib><creatorcontrib>Sabattini, Elena</creatorcontrib><creatorcontrib>Bartolommei, Sabrina</creatorcontrib><creatorcontrib>Hummel, Michael</creatorcontrib><creatorcontrib>Pileri, Stefano</creatorcontrib><creatorcontrib>Tosi, Piero</creatorcontrib><creatorcontrib>Leoncini, Lorenzo</creatorcontrib><creatorcontrib>Giordano, Antonio</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bellan, Cristiana</au><au>De Falco, Giulia</au><au>Lazzi, Stefano</au><au>Micheli, Pietro</au><au>Vicidomini, Sonia</au><au>Schürfeld, Karin</au><au>Amato, Teresa</au><au>Palumbo, Annalisa</au><au>Bagella, Luigi</au><au>Sabattini, Elena</au><au>Bartolommei, Sabrina</au><au>Hummel, Michael</au><au>Pileri, Stefano</au><au>Tosi, Piero</au><au>Leoncini, Lorenzo</au><au>Giordano, Antonio</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>CDK9/CYCLIN T1 expression during normal lymphoid differentiation and malignant transformation</atitle><jtitle>The Journal of pathology</jtitle><addtitle>J. Pathol</addtitle><date>2004-08</date><risdate>2004</risdate><volume>203</volume><issue>4</issue><spage>946</spage><epage>952</epage><pages>946-952</pages><issn>0022-3417</issn><eissn>1096-9896</eissn><coden>JPTLAS</coden><abstract>CDK9 is a member of the CDC2‐like family of kinases. Its cyclin partners are members of the CYCLIN T family (T1, T2a, and T2b) and CYCLIN K. The CDK9/CYCLIN T1 complex is very important in the differentiation programme of several cell types, controlling specific differentiation pathways. Limited data are available regarding the expression of CDK9/CYCLIN T1 in haematopoietic and lymphoid tissues. The aim of this study was to analyse the expression of the CDK9/CYCLIN T1 complex in lymphoid tissue, in order to assess its role in B‐ and T‐cell differentiation and lymphomagenesis. CDK9/CYCLIN T1 expression was found by immunohistochemistry in precursor B and T cells. In peripheral lymphoid tissues, germinal centre cells and scattered B‐ and T‐cell blasts in interfollicular areas expressed CDK9/CYCLIN T1, while mantle cells, plasma cells, and small resting T‐lymphocytes displayed no expression of either molecule. CDK9/CYCLIN T1 expression therefore appears to be related to particular stages of lymphoid differentiation/activation. CDK9 and CYCLIN T1 were highly expressed in lymphomas derived from precursor B and T cells, from germinal centre cells, such as follicular lymphomas, and from activated T cells (ie anaplastic large cell lymphomas). Hodgkin and Reed–Sternberg cells of classical Hodgkin's lymphoma also showed strong nuclear staining. Diffuse large B‐cell, Burkitt's lymphomas, and peripheral T‐cell lymphomas, among T‐cell lymphoproliferative disorders, showed a wide range of values. No expression of CDK9 or CYCLIN T1 was detected in mantle cell and marginal zone lymphomas. However, at the mRNA level, an imbalance in the CDK9/CYCLIN T1 ratio was found in follicular lymphoma and diffuse large B‐cell lymphomas with germinal centre phenotype, and in the cell lines of classical Hodgkin's lymphomas, Burkitt's lymphomas, and anaplastic large cell lymphoma, in comparison with reactive lymph nodes. These results suggest that the CDK9/CYCLIN T1 complex may affect the activation and differentiation programme of lymphoid cells. The molecular mechanism through which the CDK9/CYCLIN T1 complex is altered in malignant transformation needs to be elucidated. Copyright © 2004 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.</abstract><cop>Chichester, UK</cop><pub>John Wiley & Sons, Ltd</pub><pmid>15258998</pmid><doi>10.1002/path.1588</doi><tpages>7</tpages></addata></record> |
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| subjects | Biological and medical sciences Blotting, Western CDK9 Cell Differentiation Cell Transformation, Neoplastic - metabolism Cell Transformation, Neoplastic - pathology CYCLIN T Cyclin-Dependent Kinase 9 - genetics Cyclin-Dependent Kinase 9 - metabolism Cyclins - genetics Cyclins - metabolism differentiation Female Gene Expression Hematologic and hematopoietic diseases Humans Immunoenzyme Techniques Investigative techniques, diagnostic techniques (general aspects) Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis lymphadenitis lymphocyte lymphoma Lymphoma, B-Cell - metabolism Lymphoma, B-Cell - pathology Lymphoma, T-Cell - metabolism Lymphoma, T-Cell - pathology Male Medical sciences Neoplasm Proteins - genetics Neoplasm Proteins - metabolism Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - genetics |
| title | CDK9/CYCLIN T1 expression during normal lymphoid differentiation and malignant transformation |
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