Characterization of a complex restriction-modification system detected in Staphylococcus aureus and Streptococcus agalactiae strains isolated from infections of domestic animals
Characterization of classic type II restriction-modification systems (RMS) (restriction endonucleases and modification methyltransferases) was carried out in isolates of Staphylococcus aureus and Streptococcus agalactiae obtained from clinical material. Among the 100 isolates of S. aureus two differ...
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| Veröffentlicht in: | Folia microbiologica 2004, Vol.49 (3), p.307-314 |
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| creator | Godány, A Bukovská, G Farkasovská, J Brnáková, Z Dmitriev, A Tkáciková, E Ayele, T Mikula, I |
| description | Characterization of classic type II restriction-modification systems (RMS) (restriction endonucleases and modification methyltransferases) was carried out in isolates of Staphylococcus aureus and Streptococcus agalactiae obtained from clinical material. Among the 100 isolates of S. aureus two different RMS type II were detected. The first was expressed in isolates 32 and 33 (Sau32 I and Sau33 I); the targeting sequence was determined as 5'-GGN CC-3' (Sau96 I isoschizomer). The second was found in isolates no. 90, 93, 96*, and 98 (Sau90 I, Sau93 I, Sau96* I, Sau98 I) and enzymes recognized sequence 5'-CTY RAG-3' (SmlI isoschizomer). Analysis of 40 isolates of S. agalactiae revealed only one RMS; it was detected in two isolates (no. 16 and 23; Sag16 I and Sag23 I). Restriction endonuclease expressed by these isolates cleaved DNA in sequence 5'-CTG CA/G-3' (PstI isoschizomer). In RMS-positive S. aureus and S. agalactiae isolates plasmid DNA capable of replication in Escherichia coli and Bacillus subtilis was also detected and isolated. |
| doi_str_mv | 10.1007/BF02931048 |
| format | Article |
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Among the 100 isolates of S. aureus two different RMS type II were detected. The first was expressed in isolates 32 and 33 (Sau32 I and Sau33 I); the targeting sequence was determined as 5'-GGN CC-3' (Sau96 I isoschizomer). The second was found in isolates no. 90, 93, 96*, and 98 (Sau90 I, Sau93 I, Sau96* I, Sau98 I) and enzymes recognized sequence 5'-CTY RAG-3' (SmlI isoschizomer). Analysis of 40 isolates of S. agalactiae revealed only one RMS; it was detected in two isolates (no. 16 and 23; Sag16 I and Sag23 I). Restriction endonuclease expressed by these isolates cleaved DNA in sequence 5'-CTG CA/G-3' (PstI isoschizomer). In RMS-positive S. aureus and S. agalactiae isolates plasmid DNA capable of replication in Escherichia coli and Bacillus subtilis was also detected and isolated.</description><identifier>ISSN: 0015-5632</identifier><identifier>DOI: 10.1007/BF02931048</identifier><identifier>PMID: 15259773</identifier><language>eng</language><publisher>United States</publisher><subject>Animals ; Animals, Domestic ; Bacillus subtilis ; Deoxyribonucleases, Type II Site-Specific - genetics ; Deoxyribonucleases, Type II Site-Specific - metabolism ; DNA Restriction-Modification Enzymes - genetics ; DNA Restriction-Modification Enzymes - metabolism ; DNA, Bacterial - metabolism ; Escherichia coli ; Staphylococcal Infections - microbiology ; Staphylococcal Infections - veterinary ; Staphylococcus aureus ; Staphylococcus aureus - enzymology ; Staphylococcus aureus - isolation & purification ; Streptococcal Infections - microbiology ; Streptococcal Infections - veterinary ; Streptococcus agalactiae ; Streptococcus agalactiae - enzymology ; Streptococcus agalactiae - isolation & purification</subject><ispartof>Folia microbiologica, 2004, Vol.49 (3), p.307-314</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,4024,27923,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15259773$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Godány, A</creatorcontrib><creatorcontrib>Bukovská, G</creatorcontrib><creatorcontrib>Farkasovská, J</creatorcontrib><creatorcontrib>Brnáková, Z</creatorcontrib><creatorcontrib>Dmitriev, A</creatorcontrib><creatorcontrib>Tkáciková, E</creatorcontrib><creatorcontrib>Ayele, T</creatorcontrib><creatorcontrib>Mikula, I</creatorcontrib><title>Characterization of a complex restriction-modification system detected in Staphylococcus aureus and Streptococcus agalactiae strains isolated from infections of domestic animals</title><title>Folia microbiologica</title><addtitle>Folia Microbiol (Praha)</addtitle><description>Characterization of classic type II restriction-modification systems (RMS) (restriction endonucleases and modification methyltransferases) was carried out in isolates of Staphylococcus aureus and Streptococcus agalactiae obtained from clinical material. Among the 100 isolates of S. aureus two different RMS type II were detected. The first was expressed in isolates 32 and 33 (Sau32 I and Sau33 I); the targeting sequence was determined as 5'-GGN CC-3' (Sau96 I isoschizomer). The second was found in isolates no. 90, 93, 96*, and 98 (Sau90 I, Sau93 I, Sau96* I, Sau98 I) and enzymes recognized sequence 5'-CTY RAG-3' (SmlI isoschizomer). Analysis of 40 isolates of S. agalactiae revealed only one RMS; it was detected in two isolates (no. 16 and 23; Sag16 I and Sag23 I). Restriction endonuclease expressed by these isolates cleaved DNA in sequence 5'-CTG CA/G-3' (PstI isoschizomer). In RMS-positive S. aureus and S. agalactiae isolates plasmid DNA capable of replication in Escherichia coli and Bacillus subtilis was also detected and isolated.</description><subject>Animals</subject><subject>Animals, Domestic</subject><subject>Bacillus subtilis</subject><subject>Deoxyribonucleases, Type II Site-Specific - genetics</subject><subject>Deoxyribonucleases, Type II Site-Specific - metabolism</subject><subject>DNA Restriction-Modification Enzymes - genetics</subject><subject>DNA Restriction-Modification Enzymes - metabolism</subject><subject>DNA, Bacterial - metabolism</subject><subject>Escherichia coli</subject><subject>Staphylococcal Infections - microbiology</subject><subject>Staphylococcal Infections - veterinary</subject><subject>Staphylococcus aureus</subject><subject>Staphylococcus aureus - enzymology</subject><subject>Staphylococcus aureus - isolation & purification</subject><subject>Streptococcal Infections - microbiology</subject><subject>Streptococcal Infections - veterinary</subject><subject>Streptococcus agalactiae</subject><subject>Streptococcus agalactiae - enzymology</subject><subject>Streptococcus agalactiae - isolation & purification</subject><issn>0015-5632</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkTtPwzAUhT2AaCks_ADkiS3gR5zHCBUFpEoMwBzd-EGNkjjYiUT5V_xDHFpYmc5wzv3uuboInVFySQnJr25WhJWckrQ4QHNCqEhExtkMHYfwRkhGUs6O0IwKJso853P0tdyABzlobz9hsK7DzmDA0rV9oz-w12HwVk5G0jpljZW7VNiGQbdY6UHHYYVth58G6Dfbxkkn5RgwjF5P0qnoeN0Pf8YrNHGjBY0jHGwXsA2ugQljvGsjy-iflWEqo1wbS1gZSbaFJpygQxNFn-51gV5Wt8_L-2T9ePewvF4nPePFkHAiiJLMiDIDw0WqRF3nDLKaKFGagmW8oLVgnIGkyqS5KmQKRW4oqEJwxvkCXey4vXfvY6xQtTZI3TTQaTeGKstyxkT-f5AWhDDGSQye74Nj3WpV9T4e5LfV7zf4Nw5Njrw</recordid><startdate>2004</startdate><enddate>2004</enddate><creator>Godány, A</creator><creator>Bukovská, G</creator><creator>Farkasovská, J</creator><creator>Brnáková, Z</creator><creator>Dmitriev, A</creator><creator>Tkáciková, E</creator><creator>Ayele, T</creator><creator>Mikula, I</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7QL</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>2004</creationdate><title>Characterization of a complex restriction-modification system detected in Staphylococcus aureus and Streptococcus agalactiae strains isolated from infections of domestic animals</title><author>Godány, A ; Bukovská, G ; Farkasovská, J ; Brnáková, Z ; Dmitriev, A ; Tkáciková, E ; Ayele, T ; Mikula, I</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p238t-3050dc2f596af354d5bb72a6b0d59f826381b5232ac1df47d8c4a87f1ad853233</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Animals</topic><topic>Animals, Domestic</topic><topic>Bacillus subtilis</topic><topic>Deoxyribonucleases, Type II Site-Specific - genetics</topic><topic>Deoxyribonucleases, Type II Site-Specific - metabolism</topic><topic>DNA Restriction-Modification Enzymes - genetics</topic><topic>DNA Restriction-Modification Enzymes - metabolism</topic><topic>DNA, Bacterial - metabolism</topic><topic>Escherichia coli</topic><topic>Staphylococcal Infections - microbiology</topic><topic>Staphylococcal Infections - veterinary</topic><topic>Staphylococcus aureus</topic><topic>Staphylococcus aureus - enzymology</topic><topic>Staphylococcus aureus - isolation & purification</topic><topic>Streptococcal Infections - microbiology</topic><topic>Streptococcal Infections - veterinary</topic><topic>Streptococcus agalactiae</topic><topic>Streptococcus agalactiae - enzymology</topic><topic>Streptococcus agalactiae - isolation & purification</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Godány, A</creatorcontrib><creatorcontrib>Bukovská, G</creatorcontrib><creatorcontrib>Farkasovská, J</creatorcontrib><creatorcontrib>Brnáková, Z</creatorcontrib><creatorcontrib>Dmitriev, A</creatorcontrib><creatorcontrib>Tkáciková, E</creatorcontrib><creatorcontrib>Ayele, T</creatorcontrib><creatorcontrib>Mikula, I</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>Folia microbiologica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Godány, A</au><au>Bukovská, G</au><au>Farkasovská, J</au><au>Brnáková, Z</au><au>Dmitriev, A</au><au>Tkáciková, E</au><au>Ayele, T</au><au>Mikula, I</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of a complex restriction-modification system detected in Staphylococcus aureus and Streptococcus agalactiae strains isolated from infections of domestic animals</atitle><jtitle>Folia microbiologica</jtitle><addtitle>Folia Microbiol (Praha)</addtitle><date>2004</date><risdate>2004</risdate><volume>49</volume><issue>3</issue><spage>307</spage><epage>314</epage><pages>307-314</pages><issn>0015-5632</issn><abstract>Characterization of classic type II restriction-modification systems (RMS) (restriction endonucleases and modification methyltransferases) was carried out in isolates of Staphylococcus aureus and Streptococcus agalactiae obtained from clinical material. Among the 100 isolates of S. aureus two different RMS type II were detected. The first was expressed in isolates 32 and 33 (Sau32 I and Sau33 I); the targeting sequence was determined as 5'-GGN CC-3' (Sau96 I isoschizomer). The second was found in isolates no. 90, 93, 96*, and 98 (Sau90 I, Sau93 I, Sau96* I, Sau98 I) and enzymes recognized sequence 5'-CTY RAG-3' (SmlI isoschizomer). Analysis of 40 isolates of S. agalactiae revealed only one RMS; it was detected in two isolates (no. 16 and 23; Sag16 I and Sag23 I). Restriction endonuclease expressed by these isolates cleaved DNA in sequence 5'-CTG CA/G-3' (PstI isoschizomer). In RMS-positive S. aureus and S. agalactiae isolates plasmid DNA capable of replication in Escherichia coli and Bacillus subtilis was also detected and isolated.</abstract><cop>United States</cop><pmid>15259773</pmid><doi>10.1007/BF02931048</doi><tpages>8</tpages></addata></record> |
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| subjects | Animals Animals, Domestic Bacillus subtilis Deoxyribonucleases, Type II Site-Specific - genetics Deoxyribonucleases, Type II Site-Specific - metabolism DNA Restriction-Modification Enzymes - genetics DNA Restriction-Modification Enzymes - metabolism DNA, Bacterial - metabolism Escherichia coli Staphylococcal Infections - microbiology Staphylococcal Infections - veterinary Staphylococcus aureus Staphylococcus aureus - enzymology Staphylococcus aureus - isolation & purification Streptococcal Infections - microbiology Streptococcal Infections - veterinary Streptococcus agalactiae Streptococcus agalactiae - enzymology Streptococcus agalactiae - isolation & purification |
| title | Characterization of a complex restriction-modification system detected in Staphylococcus aureus and Streptococcus agalactiae strains isolated from infections of domestic animals |
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