In vitro sperm penetration through the zona pellucida of immature and in vitro matured oocytes using fresh, chilled and frozen canine semen
The aim of this study was to evaluate the effect of sperm cryopreservation and the maturation state of the oocyte on the time course of canine gamete interaction during co-culture for periods of 1–10 h. Semen samples were obtained by digital stimulation and ejaculates processed as fresh, chilled and...
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| Veröffentlicht in: | Animal reproduction science 2009, Vol.110 (1), p.37-45 |
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| creator | De los Reyes, Monica Palomino, Jaime de Lange, Johanna Anguita, Carla Barros, Claudio |
| description | The aim of this study was to evaluate the effect of sperm cryopreservation and the maturation state of the oocyte on the time course of canine gamete interaction during co-culture for periods of 1–10
h. Semen samples were obtained by digital stimulation and ejaculates processed as fresh, chilled and frozen samples. Sperm were co-cultured with immature or in vitro mature bitch oocytes for up to 10
h. At hourly intervals, oocytes were evaluated for sperm penetration with epifluorescence microscopy. The results were analyzed statistically using generalized linear models. Spermatozoa treatments had a significant effect on the total percentage of oocyte penetration for both types of oocytes; fresh spermatozoa showed the highest average penetration rate, while frozen sperm showed the lowest value (
p
<
0.05). At the 1st hour of co-culture, chilled and frozen dog sperm had a higher penetration percentage (
p
<
0.05) of in vitro matured canine oocytes (43.6% and 45.7%, respectively) than the fresh sperm had (33.8%). Sperm penetration was directly proportional to the time of incubation, when fresh or chilled sperm were used (
P
<
0.05); in contrast, frozen dog sperm did not change penetration rates with either immature or in vitro matured oocytes over time. There was a significant difference in the average of penetration rate between immature (47.3%) and in vitro matured oocytes (56.6%) throughout the 10
h of culturing; irrespective of sperm treatment. The optimal incubation time in terms of maximizing penetration rates probably are dependent on how spermatozoa were processed prior to fertilization. |
| doi_str_mv | 10.1016/j.anireprosci.2007.12.010 |
| format | Article |
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h. Semen samples were obtained by digital stimulation and ejaculates processed as fresh, chilled and frozen samples. Sperm were co-cultured with immature or in vitro mature bitch oocytes for up to 10
h. At hourly intervals, oocytes were evaluated for sperm penetration with epifluorescence microscopy. The results were analyzed statistically using generalized linear models. Spermatozoa treatments had a significant effect on the total percentage of oocyte penetration for both types of oocytes; fresh spermatozoa showed the highest average penetration rate, while frozen sperm showed the lowest value (
p
<
0.05). At the 1st hour of co-culture, chilled and frozen dog sperm had a higher penetration percentage (
p
<
0.05) of in vitro matured canine oocytes (43.6% and 45.7%, respectively) than the fresh sperm had (33.8%). Sperm penetration was directly proportional to the time of incubation, when fresh or chilled sperm were used (
P
<
0.05); in contrast, frozen dog sperm did not change penetration rates with either immature or in vitro matured oocytes over time. There was a significant difference in the average of penetration rate between immature (47.3%) and in vitro matured oocytes (56.6%) throughout the 10
h of culturing; irrespective of sperm treatment. The optimal incubation time in terms of maximizing penetration rates probably are dependent on how spermatozoa were processed prior to fertilization.</description><identifier>ISSN: 0378-4320</identifier><identifier>EISSN: 1873-2232</identifier><identifier>DOI: 10.1016/j.anireprosci.2007.12.010</identifier><identifier>PMID: 18258391</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; artificial insemination ; Capacitation ; conception ; cooling ; Cryopreservation ; Cryopreservation - methods ; Cryopreservation - veterinary ; Dog sperm ; dogs ; Dogs - physiology ; Female ; fertilization (reproduction) ; Fertilization in Vitro - veterinary ; freeze-thaw cycles ; Gamete interaction ; germ cells ; germplasm conservation ; in vitro fertilization ; Male ; male fertility ; male reproductive system ; males ; Microscopy, Fluorescence - veterinary ; oocytes ; Oocytes - physiology ; oogenesis ; Semen Preservation - methods ; Semen Preservation - veterinary ; Sperm-Ovum Interactions - physiology ; spermatozoa ; zona pellucida ; Zona Pellucida - physiology</subject><ispartof>Animal reproduction science, 2009, Vol.110 (1), p.37-45</ispartof><rights>2007 Elsevier B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c399t-4575c05c4b1296ae67736012aca8b66f1d60413870bf40c9d49c3e7fd633c1f53</citedby><cites>FETCH-LOGICAL-c399t-4575c05c4b1296ae67736012aca8b66f1d60413870bf40c9d49c3e7fd633c1f53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0378432007004241$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,4010,27900,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18258391$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>De los Reyes, Monica</creatorcontrib><creatorcontrib>Palomino, Jaime</creatorcontrib><creatorcontrib>de Lange, Johanna</creatorcontrib><creatorcontrib>Anguita, Carla</creatorcontrib><creatorcontrib>Barros, Claudio</creatorcontrib><title>In vitro sperm penetration through the zona pellucida of immature and in vitro matured oocytes using fresh, chilled and frozen canine semen</title><title>Animal reproduction science</title><addtitle>Anim Reprod Sci</addtitle><description>The aim of this study was to evaluate the effect of sperm cryopreservation and the maturation state of the oocyte on the time course of canine gamete interaction during co-culture for periods of 1–10
h. Semen samples were obtained by digital stimulation and ejaculates processed as fresh, chilled and frozen samples. Sperm were co-cultured with immature or in vitro mature bitch oocytes for up to 10
h. At hourly intervals, oocytes were evaluated for sperm penetration with epifluorescence microscopy. The results were analyzed statistically using generalized linear models. Spermatozoa treatments had a significant effect on the total percentage of oocyte penetration for both types of oocytes; fresh spermatozoa showed the highest average penetration rate, while frozen sperm showed the lowest value (
p
<
0.05). At the 1st hour of co-culture, chilled and frozen dog sperm had a higher penetration percentage (
p
<
0.05) of in vitro matured canine oocytes (43.6% and 45.7%, respectively) than the fresh sperm had (33.8%). Sperm penetration was directly proportional to the time of incubation, when fresh or chilled sperm were used (
P
<
0.05); in contrast, frozen dog sperm did not change penetration rates with either immature or in vitro matured oocytes over time. There was a significant difference in the average of penetration rate between immature (47.3%) and in vitro matured oocytes (56.6%) throughout the 10
h of culturing; irrespective of sperm treatment. The optimal incubation time in terms of maximizing penetration rates probably are dependent on how spermatozoa were processed prior to fertilization.</description><subject>Animals</subject><subject>artificial insemination</subject><subject>Capacitation</subject><subject>conception</subject><subject>cooling</subject><subject>Cryopreservation</subject><subject>Cryopreservation - methods</subject><subject>Cryopreservation - veterinary</subject><subject>Dog sperm</subject><subject>dogs</subject><subject>Dogs - physiology</subject><subject>Female</subject><subject>fertilization (reproduction)</subject><subject>Fertilization in Vitro - veterinary</subject><subject>freeze-thaw cycles</subject><subject>Gamete interaction</subject><subject>germ cells</subject><subject>germplasm conservation</subject><subject>in vitro fertilization</subject><subject>Male</subject><subject>male fertility</subject><subject>male reproductive system</subject><subject>males</subject><subject>Microscopy, Fluorescence - veterinary</subject><subject>oocytes</subject><subject>Oocytes - physiology</subject><subject>oogenesis</subject><subject>Semen Preservation - methods</subject><subject>Semen Preservation - veterinary</subject><subject>Sperm-Ovum Interactions - physiology</subject><subject>spermatozoa</subject><subject>zona pellucida</subject><subject>Zona Pellucida - physiology</subject><issn>0378-4320</issn><issn>1873-2232</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc-O0zAQxi0EYsvCK4C5cCLBYyd2ckQVf1ZaiQPs2XKdcesqsYudrLT7Crw0rlIER04jeX7fjOf7CHkLrAYG8sOxNsEnPKWYra85Y6oGXjNgT8gGOiUqzgV_SjZMqK5qBGdX5EXOR1ZAKfvn5Ao63naihw35dRPovZ9TpPmEaaInDDgnM_sY6HxIcdkfSkX6GIMpzXFcrB8MjY76aTLzkpCaMFD_Z8r6NtAY7cOMmS7Zhz11CfPhPbUHP46leVa4FB8xUFsuCUgzThhekmfOjBlfXeo1ufv86cf2a3X77cvN9uNtZUXfz1XTqtay1jY74L00KJUSkgE31nQ7KR0MkjUgOsV2rmG2H5reClRukEJYcK24Ju_WucXAnwvmWU8-23KbCRiXrKVUHEB1BexX0Banc0KnT8lPJj1oYPqchD7qf5LQ5yQ0cF2SKNrXlyXLbsLhr_JifQHerIAzUZt98lnffecMBINWctbJQmxXAosZ9x6TLkswWBzKSjvrIfr_-Mhvfxqr8A</recordid><startdate>2009</startdate><enddate>2009</enddate><creator>De los Reyes, Monica</creator><creator>Palomino, Jaime</creator><creator>de Lange, Johanna</creator><creator>Anguita, Carla</creator><creator>Barros, Claudio</creator><general>Elsevier B.V</general><general>[Amsterdam]: Elsevier Science</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>2009</creationdate><title>In vitro sperm penetration through the zona pellucida of immature and in vitro matured oocytes using fresh, chilled and frozen canine semen</title><author>De los Reyes, Monica ; Palomino, Jaime ; de Lange, Johanna ; Anguita, Carla ; Barros, Claudio</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c399t-4575c05c4b1296ae67736012aca8b66f1d60413870bf40c9d49c3e7fd633c1f53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Animals</topic><topic>artificial insemination</topic><topic>Capacitation</topic><topic>conception</topic><topic>cooling</topic><topic>Cryopreservation</topic><topic>Cryopreservation - methods</topic><topic>Cryopreservation - veterinary</topic><topic>Dog sperm</topic><topic>dogs</topic><topic>Dogs - physiology</topic><topic>Female</topic><topic>fertilization (reproduction)</topic><topic>Fertilization in Vitro - veterinary</topic><topic>freeze-thaw cycles</topic><topic>Gamete interaction</topic><topic>germ cells</topic><topic>germplasm conservation</topic><topic>in vitro fertilization</topic><topic>Male</topic><topic>male fertility</topic><topic>male reproductive system</topic><topic>males</topic><topic>Microscopy, Fluorescence - veterinary</topic><topic>oocytes</topic><topic>Oocytes - physiology</topic><topic>oogenesis</topic><topic>Semen Preservation - methods</topic><topic>Semen Preservation - veterinary</topic><topic>Sperm-Ovum Interactions - physiology</topic><topic>spermatozoa</topic><topic>zona pellucida</topic><topic>Zona Pellucida - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>De los Reyes, Monica</creatorcontrib><creatorcontrib>Palomino, Jaime</creatorcontrib><creatorcontrib>de Lange, Johanna</creatorcontrib><creatorcontrib>Anguita, Carla</creatorcontrib><creatorcontrib>Barros, Claudio</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Animal reproduction science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>De los Reyes, Monica</au><au>Palomino, Jaime</au><au>de Lange, Johanna</au><au>Anguita, Carla</au><au>Barros, Claudio</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In vitro sperm penetration through the zona pellucida of immature and in vitro matured oocytes using fresh, chilled and frozen canine semen</atitle><jtitle>Animal reproduction science</jtitle><addtitle>Anim Reprod Sci</addtitle><date>2009</date><risdate>2009</risdate><volume>110</volume><issue>1</issue><spage>37</spage><epage>45</epage><pages>37-45</pages><issn>0378-4320</issn><eissn>1873-2232</eissn><abstract>The aim of this study was to evaluate the effect of sperm cryopreservation and the maturation state of the oocyte on the time course of canine gamete interaction during co-culture for periods of 1–10
h. Semen samples were obtained by digital stimulation and ejaculates processed as fresh, chilled and frozen samples. Sperm were co-cultured with immature or in vitro mature bitch oocytes for up to 10
h. At hourly intervals, oocytes were evaluated for sperm penetration with epifluorescence microscopy. The results were analyzed statistically using generalized linear models. Spermatozoa treatments had a significant effect on the total percentage of oocyte penetration for both types of oocytes; fresh spermatozoa showed the highest average penetration rate, while frozen sperm showed the lowest value (
p
<
0.05). At the 1st hour of co-culture, chilled and frozen dog sperm had a higher penetration percentage (
p
<
0.05) of in vitro matured canine oocytes (43.6% and 45.7%, respectively) than the fresh sperm had (33.8%). Sperm penetration was directly proportional to the time of incubation, when fresh or chilled sperm were used (
P
<
0.05); in contrast, frozen dog sperm did not change penetration rates with either immature or in vitro matured oocytes over time. There was a significant difference in the average of penetration rate between immature (47.3%) and in vitro matured oocytes (56.6%) throughout the 10
h of culturing; irrespective of sperm treatment. The optimal incubation time in terms of maximizing penetration rates probably are dependent on how spermatozoa were processed prior to fertilization.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>18258391</pmid><doi>10.1016/j.anireprosci.2007.12.010</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0378-4320 |
| ispartof | Animal reproduction science, 2009, Vol.110 (1), p.37-45 |
| issn | 0378-4320 1873-2232 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_66721178 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals artificial insemination Capacitation conception cooling Cryopreservation Cryopreservation - methods Cryopreservation - veterinary Dog sperm dogs Dogs - physiology Female fertilization (reproduction) Fertilization in Vitro - veterinary freeze-thaw cycles Gamete interaction germ cells germplasm conservation in vitro fertilization Male male fertility male reproductive system males Microscopy, Fluorescence - veterinary oocytes Oocytes - physiology oogenesis Semen Preservation - methods Semen Preservation - veterinary Sperm-Ovum Interactions - physiology spermatozoa zona pellucida Zona Pellucida - physiology |
| title | In vitro sperm penetration through the zona pellucida of immature and in vitro matured oocytes using fresh, chilled and frozen canine semen |
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