Efficient extraction of intracellular reduced glutathione from fermentation broth of Saccharomyces cerevisiae by ethanol

Reduced glutathione (GSH) from fermentation broth of Saccharomyces cerevisiae was extracted with ethanol without disruption of the cells. The effects of ethanol concentration, extraction temperature and extraction time were assessed by using 23 full factorial designs (FFD). Preliminary studies showe...

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Veröffentlicht in:	Bioresource technology 2009-01, Vol.100 (2), p.1011-1014
Hauptverfasser:	Xiong, Zhi-Qiang, Guo, Mei-Jin, Guo, Yuan-Xin, Chu, Ju, Zhuang, Ying-Ping, Zhang, Si-Liang
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Biotechnology Chemical Fractionation - methods Culture Media - chemistry Culture Media - isolation & purification Ethanol - chemistry Ethanol extraction Fermentation Full factorial designs Fundamental and applied biological sciences. Psychology Glutathione - isolation & purification Glutathione - metabolism Methods. Procedures. Technologies Microbial engineering. Fermentation and microbial culture technology Reduced glutathione Saccharomyces cerevisiae Saccharomyces cerevisiae - metabolism
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