Combined demyelination plus Schwann cell transplantation therapy increases spread of cells and axonal regeneration following contusion injury

Several cell populations have been shown to provide a permissive environment for axonal extension following transplantation to injury sites. The limited spread of transplanted cells from implantation sites in the mature CNS, and the superior substrate and trophic environment that they provide, likel...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Journal of neurotrauma 2004-06, Vol.21 (6), p.775-788
Hauptverfasser: AZANCHI, Roya, BERNAL, Giovanna, GUPTA, Ranjan, KEIRSTEAD, Hans S
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 788
container_issue 6
container_start_page 775
container_title Journal of neurotrauma
container_volume 21
creator AZANCHI, Roya
BERNAL, Giovanna
GUPTA, Ranjan
KEIRSTEAD, Hans S
description Several cell populations have been shown to provide a permissive environment for axonal extension following transplantation to injury sites. The limited spread of transplanted cells from implantation sites in the mature CNS, and the superior substrate and trophic environment that they provide, likely contribute to the fact that few transplantation-based therapies have elicited axonal extension beyond the transplant. The aim of this study was to determine whether (1) regions of demyelination cranial and caudal to a spinal cord injury site would improve the spread of Schwann cells transplanted into the site of injury, and (2) whether this combination therapy was associated with improved anatomical regeneration. Three days following contusion injury, anti-galactocerebroside antibodies plus complement proteins were injected into the dorsal column cranial and caudal to the injury site, resulting in complete and well defined regions of demyelination that extended 8 mm either side of the injury site. One day later, naïve Schwann cells in suspension were injected into the contusion site. Transplanted Schwann cells homogeneously redistributed throughout the contusion site and the adjacent regions of demyelination cranial and caudal to the contusion site, providing a long-distance prospective path for repair that was free of myelin and contained transplanted cells. Animals that received demyelination plus transplantation therapy, but not untreated or single-treatment groups, exhibited robust axonal regeneration beyond the contusion site within the treated dorsal column. Axonal regeneration in these animals was not associated with an improvement in locomotor ability. These findings suggest that this combination therapy may overcome a central limitation of transplant strategies in which the permissive environment provided remains at the implantation site.
doi_str_mv 10.1089/0897715041269696
format Article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_66715662</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1149825291</sourcerecordid><originalsourceid>FETCH-LOGICAL-c352t-5501e149be8d6b21d6304faaa665a9423dbc406e50eee5c4f67f35ec03dc7ea13</originalsourceid><addsrcrecordid>eNpdkU2LFDEQhoMo7rh69yRBcG-t-e7powy6Cgse1HNTnVTvZkgnbdLNOj_C_2xmZ0BZQkiRet6iql5CXnP2nrNt96HetuWaKS5MV88TsuFat03HlHhKNsd0U_P8grwoZc8Yl0a0z8kF10LLLVMb8meXpsFHdNThdMDgIyw-RTqHtdDv9u4eYqQWQ6BLhljmAHE5EcsdZpgP1EebEQoWWuYaOJrGB0GhEB2F3ylCoBlvMVb-QTmmENK9j7fUpris5fjn437Nh5fk2Qih4Kvze0l-fv70Y_elufl2_XX38aaxUoul0Zpx5KobcOvMILgzkqkRAIzR0Ckh3WAVM6gZImqrRtOOUqNl0tkWgctLcnWqO-f0a8Wy9JMvx6YhYlpLb0zdmjGigm8fgfu05jpR6QVTWrBuqyvETpDNqZSMYz9nP0E-9Jz1R5_6xz5VyZtz3XWY0P0TnI2pwLszAMVCGOvyrS__cZ1Uwkj5F4zgngE</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>204520985</pqid></control><display><type>article</type><title>Combined demyelination plus Schwann cell transplantation therapy increases spread of cells and axonal regeneration following contusion injury</title><source>Mary Ann Liebert Online Subscription</source><source>MEDLINE</source><creator>AZANCHI, Roya ; BERNAL, Giovanna ; GUPTA, Ranjan ; KEIRSTEAD, Hans S</creator><creatorcontrib>AZANCHI, Roya ; BERNAL, Giovanna ; GUPTA, Ranjan ; KEIRSTEAD, Hans S</creatorcontrib><description>Several cell populations have been shown to provide a permissive environment for axonal extension following transplantation to injury sites. The limited spread of transplanted cells from implantation sites in the mature CNS, and the superior substrate and trophic environment that they provide, likely contribute to the fact that few transplantation-based therapies have elicited axonal extension beyond the transplant. The aim of this study was to determine whether (1) regions of demyelination cranial and caudal to a spinal cord injury site would improve the spread of Schwann cells transplanted into the site of injury, and (2) whether this combination therapy was associated with improved anatomical regeneration. Three days following contusion injury, anti-galactocerebroside antibodies plus complement proteins were injected into the dorsal column cranial and caudal to the injury site, resulting in complete and well defined regions of demyelination that extended 8 mm either side of the injury site. One day later, naïve Schwann cells in suspension were injected into the contusion site. Transplanted Schwann cells homogeneously redistributed throughout the contusion site and the adjacent regions of demyelination cranial and caudal to the contusion site, providing a long-distance prospective path for repair that was free of myelin and contained transplanted cells. Animals that received demyelination plus transplantation therapy, but not untreated or single-treatment groups, exhibited robust axonal regeneration beyond the contusion site within the treated dorsal column. Axonal regeneration in these animals was not associated with an improvement in locomotor ability. These findings suggest that this combination therapy may overcome a central limitation of transplant strategies in which the permissive environment provided remains at the implantation site.</description><identifier>ISSN: 0897-7151</identifier><identifier>EISSN: 1557-9042</identifier><identifier>DOI: 10.1089/0897715041269696</identifier><identifier>PMID: 15253804</identifier><identifier>CODEN: JNEUE4</identifier><language>eng</language><publisher>Larchmont, NY: Liebert</publisher><subject>Animals ; Antibodies ; Antibodies - immunology ; Axons - physiology ; Biological and medical sciences ; Cavitation ; Cell Movement - physiology ; Contusions ; Demyelinating Diseases ; Female ; Galactosylceramides - immunology ; Growth factors ; Immunology ; Injuries of the nervous system and the skull. Diseases due to physical agents ; Laboratories ; Medical sciences ; Myelin Sheath - immunology ; Nerve Regeneration - physiology ; Neurosciences ; Proteins ; Rats ; Rats, Sprague-Dawley ; Schwann Cells - physiology ; Schwann Cells - transplantation ; Spinal cord injuries ; Spinal Cord Injuries - therapy ; Surgery ; Transplants &amp; implants ; Traumas. Diseases due to physical agents</subject><ispartof>Journal of neurotrauma, 2004-06, Vol.21 (6), p.775-788</ispartof><rights>2004 INIST-CNRS</rights><rights>(©) © 2004 Mary Ann Liebert, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c352t-5501e149be8d6b21d6304faaa665a9423dbc406e50eee5c4f67f35ec03dc7ea13</citedby><cites>FETCH-LOGICAL-c352t-5501e149be8d6b21d6304faaa665a9423dbc406e50eee5c4f67f35ec03dc7ea13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,3042,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&amp;idt=15934263$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15253804$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>AZANCHI, Roya</creatorcontrib><creatorcontrib>BERNAL, Giovanna</creatorcontrib><creatorcontrib>GUPTA, Ranjan</creatorcontrib><creatorcontrib>KEIRSTEAD, Hans S</creatorcontrib><title>Combined demyelination plus Schwann cell transplantation therapy increases spread of cells and axonal regeneration following contusion injury</title><title>Journal of neurotrauma</title><addtitle>J Neurotrauma</addtitle><description>Several cell populations have been shown to provide a permissive environment for axonal extension following transplantation to injury sites. The limited spread of transplanted cells from implantation sites in the mature CNS, and the superior substrate and trophic environment that they provide, likely contribute to the fact that few transplantation-based therapies have elicited axonal extension beyond the transplant. The aim of this study was to determine whether (1) regions of demyelination cranial and caudal to a spinal cord injury site would improve the spread of Schwann cells transplanted into the site of injury, and (2) whether this combination therapy was associated with improved anatomical regeneration. Three days following contusion injury, anti-galactocerebroside antibodies plus complement proteins were injected into the dorsal column cranial and caudal to the injury site, resulting in complete and well defined regions of demyelination that extended 8 mm either side of the injury site. One day later, naïve Schwann cells in suspension were injected into the contusion site. Transplanted Schwann cells homogeneously redistributed throughout the contusion site and the adjacent regions of demyelination cranial and caudal to the contusion site, providing a long-distance prospective path for repair that was free of myelin and contained transplanted cells. Animals that received demyelination plus transplantation therapy, but not untreated or single-treatment groups, exhibited robust axonal regeneration beyond the contusion site within the treated dorsal column. Axonal regeneration in these animals was not associated with an improvement in locomotor ability. These findings suggest that this combination therapy may overcome a central limitation of transplant strategies in which the permissive environment provided remains at the implantation site.</description><subject>Animals</subject><subject>Antibodies</subject><subject>Antibodies - immunology</subject><subject>Axons - physiology</subject><subject>Biological and medical sciences</subject><subject>Cavitation</subject><subject>Cell Movement - physiology</subject><subject>Contusions</subject><subject>Demyelinating Diseases</subject><subject>Female</subject><subject>Galactosylceramides - immunology</subject><subject>Growth factors</subject><subject>Immunology</subject><subject>Injuries of the nervous system and the skull. Diseases due to physical agents</subject><subject>Laboratories</subject><subject>Medical sciences</subject><subject>Myelin Sheath - immunology</subject><subject>Nerve Regeneration - physiology</subject><subject>Neurosciences</subject><subject>Proteins</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Schwann Cells - physiology</subject><subject>Schwann Cells - transplantation</subject><subject>Spinal cord injuries</subject><subject>Spinal Cord Injuries - therapy</subject><subject>Surgery</subject><subject>Transplants &amp; implants</subject><subject>Traumas. Diseases due to physical agents</subject><issn>0897-7151</issn><issn>1557-9042</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNpdkU2LFDEQhoMo7rh69yRBcG-t-e7powy6Cgse1HNTnVTvZkgnbdLNOj_C_2xmZ0BZQkiRet6iql5CXnP2nrNt96HetuWaKS5MV88TsuFat03HlHhKNsd0U_P8grwoZc8Yl0a0z8kF10LLLVMb8meXpsFHdNThdMDgIyw-RTqHtdDv9u4eYqQWQ6BLhljmAHE5EcsdZpgP1EebEQoWWuYaOJrGB0GhEB2F3ylCoBlvMVb-QTmmENK9j7fUpris5fjn437Nh5fk2Qih4Kvze0l-fv70Y_elufl2_XX38aaxUoul0Zpx5KobcOvMILgzkqkRAIzR0Ckh3WAVM6gZImqrRtOOUqNl0tkWgctLcnWqO-f0a8Wy9JMvx6YhYlpLb0zdmjGigm8fgfu05jpR6QVTWrBuqyvETpDNqZSMYz9nP0E-9Jz1R5_6xz5VyZtz3XWY0P0TnI2pwLszAMVCGOvyrS__cZ1Uwkj5F4zgngE</recordid><startdate>20040601</startdate><enddate>20040601</enddate><creator>AZANCHI, Roya</creator><creator>BERNAL, Giovanna</creator><creator>GUPTA, Ranjan</creator><creator>KEIRSTEAD, Hans S</creator><general>Liebert</general><general>Mary Ann Liebert, Inc</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7TK</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>88G</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>K9.</scope><scope>KB0</scope><scope>M0S</scope><scope>M1P</scope><scope>M2M</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PSYQQ</scope><scope>Q9U</scope><scope>7X8</scope></search><sort><creationdate>20040601</creationdate><title>Combined demyelination plus Schwann cell transplantation therapy increases spread of cells and axonal regeneration following contusion injury</title><author>AZANCHI, Roya ; BERNAL, Giovanna ; GUPTA, Ranjan ; KEIRSTEAD, Hans S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c352t-5501e149be8d6b21d6304faaa665a9423dbc406e50eee5c4f67f35ec03dc7ea13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Animals</topic><topic>Antibodies</topic><topic>Antibodies - immunology</topic><topic>Axons - physiology</topic><topic>Biological and medical sciences</topic><topic>Cavitation</topic><topic>Cell Movement - physiology</topic><topic>Contusions</topic><topic>Demyelinating Diseases</topic><topic>Female</topic><topic>Galactosylceramides - immunology</topic><topic>Growth factors</topic><topic>Immunology</topic><topic>Injuries of the nervous system and the skull. Diseases due to physical agents</topic><topic>Laboratories</topic><topic>Medical sciences</topic><topic>Myelin Sheath - immunology</topic><topic>Nerve Regeneration - physiology</topic><topic>Neurosciences</topic><topic>Proteins</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Schwann Cells - physiology</topic><topic>Schwann Cells - transplantation</topic><topic>Spinal cord injuries</topic><topic>Spinal Cord Injuries - therapy</topic><topic>Surgery</topic><topic>Transplants &amp; implants</topic><topic>Traumas. Diseases due to physical agents</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>AZANCHI, Roya</creatorcontrib><creatorcontrib>BERNAL, Giovanna</creatorcontrib><creatorcontrib>GUPTA, Ranjan</creatorcontrib><creatorcontrib>KEIRSTEAD, Hans S</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nursing &amp; Allied Health Database</collection><collection>Neurosciences Abstracts</collection><collection>Health &amp; Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Psychology Database (Alumni)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>Nursing &amp; Allied Health Database (Alumni Edition)</collection><collection>Health &amp; Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Psychology Database</collection><collection>Nursing &amp; Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest One Psychology</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of neurotrauma</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>AZANCHI, Roya</au><au>BERNAL, Giovanna</au><au>GUPTA, Ranjan</au><au>KEIRSTEAD, Hans S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Combined demyelination plus Schwann cell transplantation therapy increases spread of cells and axonal regeneration following contusion injury</atitle><jtitle>Journal of neurotrauma</jtitle><addtitle>J Neurotrauma</addtitle><date>2004-06-01</date><risdate>2004</risdate><volume>21</volume><issue>6</issue><spage>775</spage><epage>788</epage><pages>775-788</pages><issn>0897-7151</issn><eissn>1557-9042</eissn><coden>JNEUE4</coden><abstract>Several cell populations have been shown to provide a permissive environment for axonal extension following transplantation to injury sites. The limited spread of transplanted cells from implantation sites in the mature CNS, and the superior substrate and trophic environment that they provide, likely contribute to the fact that few transplantation-based therapies have elicited axonal extension beyond the transplant. The aim of this study was to determine whether (1) regions of demyelination cranial and caudal to a spinal cord injury site would improve the spread of Schwann cells transplanted into the site of injury, and (2) whether this combination therapy was associated with improved anatomical regeneration. Three days following contusion injury, anti-galactocerebroside antibodies plus complement proteins were injected into the dorsal column cranial and caudal to the injury site, resulting in complete and well defined regions of demyelination that extended 8 mm either side of the injury site. One day later, naïve Schwann cells in suspension were injected into the contusion site. Transplanted Schwann cells homogeneously redistributed throughout the contusion site and the adjacent regions of demyelination cranial and caudal to the contusion site, providing a long-distance prospective path for repair that was free of myelin and contained transplanted cells. Animals that received demyelination plus transplantation therapy, but not untreated or single-treatment groups, exhibited robust axonal regeneration beyond the contusion site within the treated dorsal column. Axonal regeneration in these animals was not associated with an improvement in locomotor ability. These findings suggest that this combination therapy may overcome a central limitation of transplant strategies in which the permissive environment provided remains at the implantation site.</abstract><cop>Larchmont, NY</cop><pub>Liebert</pub><pmid>15253804</pmid><doi>10.1089/0897715041269696</doi><tpages>14</tpages></addata></record>
fulltext fulltext
identifier ISSN: 0897-7151
ispartof Journal of neurotrauma, 2004-06, Vol.21 (6), p.775-788
issn 0897-7151
1557-9042
language eng
recordid cdi_proquest_miscellaneous_66715662
source Mary Ann Liebert Online Subscription; MEDLINE
subjects Animals
Antibodies
Antibodies - immunology
Axons - physiology
Biological and medical sciences
Cavitation
Cell Movement - physiology
Contusions
Demyelinating Diseases
Female
Galactosylceramides - immunology
Growth factors
Immunology
Injuries of the nervous system and the skull. Diseases due to physical agents
Laboratories
Medical sciences
Myelin Sheath - immunology
Nerve Regeneration - physiology
Neurosciences
Proteins
Rats
Rats, Sprague-Dawley
Schwann Cells - physiology
Schwann Cells - transplantation
Spinal cord injuries
Spinal Cord Injuries - therapy
Surgery
Transplants & implants
Traumas. Diseases due to physical agents
title Combined demyelination plus Schwann cell transplantation therapy increases spread of cells and axonal regeneration following contusion injury
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T03%3A19%3A59IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Combined%20demyelination%20plus%20Schwann%20cell%20transplantation%20therapy%20increases%20spread%20of%20cells%20and%20axonal%20regeneration%20following%20contusion%20injury&rft.jtitle=Journal%20of%20neurotrauma&rft.au=AZANCHI,%20Roya&rft.date=2004-06-01&rft.volume=21&rft.issue=6&rft.spage=775&rft.epage=788&rft.pages=775-788&rft.issn=0897-7151&rft.eissn=1557-9042&rft.coden=JNEUE4&rft_id=info:doi/10.1089/0897715041269696&rft_dat=%3Cproquest_cross%3E1149825291%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=204520985&rft_id=info:pmid/15253804&rfr_iscdi=true