Development of immunofiltration assay by light addressable potentiometric sensor with genetically biotinylated recombinant antibody for rapid identification of Venezuelan equine encephalitis virus

A genetically biotinylated single chain fragment variable antibody (scFv) against Venezuelan equine encephalitis virus (VEE) was applied in a system consisting of an immunofiltration enzyme assay (IFA) with a light addressable potentiometric sensor (LAPS) for the rapid identification of VEE. The IFA...

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Veröffentlicht in:	Journal of immunological methods 2004-06, Vol.289 (1), p.27-35
Hauptverfasser:	Hu, Wei-Gang, Thompson, H.Gail, Alvi, Azhar Z, Nagata, Les P, Suresh, Mavanur R, Fulton, R.Elaine
Format:	Artikel
Sprache:	eng
Schlagworte:	Antibodies, Monoclonal - chemistry Antibodies, Monoclonal - genetics Antibodies, Monoclonal - immunology Antibodies, Viral - chemistry Antibodies, Viral - genetics Antibodies, Viral - immunology Biological and medical sciences Biosensing Techniques Biotinylation Encephalitis Virus, Venezuelan Equine - immunology Encephalitis Virus, Venezuelan Equine - isolation & purification Enzyme-Linked Immunosorbent Assay Filtration Fluorescent Antibody Technique Fundamental and applied biological sciences. Psychology Fundamental immunology Genetically biotinylated recombinant antibody Identification Immunofiltration assay Immunoglobulin Variable Region - chemistry Immunoglobulin Variable Region - genetics Immunoglobulin Variable Region - immunology Light Light addressable potentiometric sensor Microbiology Molecular immunology Potentiometry - methods Recombinant Proteins - chemistry Recombinant Proteins - genetics Recombinant Proteins - immunology Sensitivity and Specificity Techniques Techniques used in virology Venezuelan equine encephalitis virus Virology
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