Identification of Bacillus anthracis by multiprobe microarray hybridization
We have developed a rapid assay based on microarray analysis of amplified genetic markers for reliable identification of Bacillus anthracis and its discrimination from other closely related bacterial species of the Bacillus cereus group. By combining polymerase chain reaction (PCR) amplification of...
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| Veröffentlicht in: | Diagnostic microbiology and infectious disease 2004-07, Vol.49 (3), p.163-171 |
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| creator | Volokhov, Dmitriy Pomerantsev, Andrei Kivovich, Violetta Rasooly, Avraham Chizhikov, Vladimir |
| description | We have developed a rapid assay based on microarray analysis of amplified genetic markers for reliable identification of
Bacillus anthracis and its discrimination from other closely related bacterial species of the
Bacillus cereus group. By combining polymerase chain reaction (PCR) amplification of six
B. anthracis-specific genes (plasmid-associated genes encoding virulence factors (
cyaA,
pagA,
lef, and
capA,
capB,
capC) and one chromosomal marker BA-5449) with analysis of amplicons by microarray hybridization, we were able to unambiguously identify and discriminate
B. anthracis among other closely related species.
Bacillus identification relied on hybridization with multiple individual microarray oligonucleotide probes (oligoprobes) specific to each target
B. anthracis gene. Evaluation of the assay was conducted using several
B. anthracis strains (with or without pXO1 and pXO2 plasmids) as well as over 50 other species phylogenetically related to
B. anthracis, including
B. cereus,
B. thuringiensis,
B. mycoides, and
B. subtilis. The developed microarray analysis of amplified genetic markers protocol provides an efficient method for (i) unambiguous identification and discrimination of
B. anthracis from other
Bacillus species and (ii) distinguishing between plasmid-containing and plasmid-free
Bacillus anthracis strains. |
| doi_str_mv | 10.1016/j.diagmicrobio.2004.03.015 |
| format | Article |
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Bacillus anthracis and its discrimination from other closely related bacterial species of the
Bacillus cereus group. By combining polymerase chain reaction (PCR) amplification of six
B. anthracis-specific genes (plasmid-associated genes encoding virulence factors (
cyaA,
pagA,
lef, and
capA,
capB,
capC) and one chromosomal marker BA-5449) with analysis of amplicons by microarray hybridization, we were able to unambiguously identify and discriminate
B. anthracis among other closely related species.
Bacillus identification relied on hybridization with multiple individual microarray oligonucleotide probes (oligoprobes) specific to each target
B. anthracis gene. Evaluation of the assay was conducted using several
B. anthracis strains (with or without pXO1 and pXO2 plasmids) as well as over 50 other species phylogenetically related to
B. anthracis, including
B. cereus,
B. thuringiensis,
B. mycoides, and
B. subtilis. The developed microarray analysis of amplified genetic markers protocol provides an efficient method for (i) unambiguous identification and discrimination of
B. anthracis from other
Bacillus species and (ii) distinguishing between plasmid-containing and plasmid-free
Bacillus anthracis strains.</description><identifier>ISSN: 0732-8893</identifier><identifier>EISSN: 1879-0070</identifier><identifier>DOI: 10.1016/j.diagmicrobio.2004.03.015</identifier><identifier>PMID: 15246505</identifier><identifier>CODEN: DMIDDZ</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>Anthrax toxins ; Bacillus anthracis ; Bacillus anthracis - classification ; Bacillus anthracis - genetics ; Bacillus anthracis - pathogenicity ; Bacillus cereus ; Bacillus cereus - classification ; Bacillus cereus - genetics ; Bacillus cereus - pathogenicity ; Base Sequence ; Biological and medical sciences ; DNA Probes ; DNA, Bacterial - analysis ; Female ; Fundamental and applied biological sciences. Psychology ; Humans ; Infectious diseases ; Male ; Medical sciences ; Microbiology ; Molecular Sequence Data ; Multiplex PCR ; Oligonucleotide Array Sequence Analysis ; Polymerase Chain Reaction - methods ; pXO1 ; pXO2 ; Sensitivity and Specificity ; Virulence factors</subject><ispartof>Diagnostic microbiology and infectious disease, 2004-07, Vol.49 (3), p.163-171</ispartof><rights>2004 Elsevier Inc.</rights><rights>2004 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c437t-8fe7474f2d66aea734bedc5b3165b2d6c0e92c93565d5eaa1e17570b37b7b4423</citedby><cites>FETCH-LOGICAL-c437t-8fe7474f2d66aea734bedc5b3165b2d6c0e92c93565d5eaa1e17570b37b7b4423</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.diagmicrobio.2004.03.015$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15978886$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15246505$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Volokhov, Dmitriy</creatorcontrib><creatorcontrib>Pomerantsev, Andrei</creatorcontrib><creatorcontrib>Kivovich, Violetta</creatorcontrib><creatorcontrib>Rasooly, Avraham</creatorcontrib><creatorcontrib>Chizhikov, Vladimir</creatorcontrib><title>Identification of Bacillus anthracis by multiprobe microarray hybridization</title><title>Diagnostic microbiology and infectious disease</title><addtitle>Diagn Microbiol Infect Dis</addtitle><description>We have developed a rapid assay based on microarray analysis of amplified genetic markers for reliable identification of
Bacillus anthracis and its discrimination from other closely related bacterial species of the
Bacillus cereus group. By combining polymerase chain reaction (PCR) amplification of six
B. anthracis-specific genes (plasmid-associated genes encoding virulence factors (
cyaA,
pagA,
lef, and
capA,
capB,
capC) and one chromosomal marker BA-5449) with analysis of amplicons by microarray hybridization, we were able to unambiguously identify and discriminate
B. anthracis among other closely related species.
Bacillus identification relied on hybridization with multiple individual microarray oligonucleotide probes (oligoprobes) specific to each target
B. anthracis gene. Evaluation of the assay was conducted using several
B. anthracis strains (with or without pXO1 and pXO2 plasmids) as well as over 50 other species phylogenetically related to
B. anthracis, including
B. cereus,
B. thuringiensis,
B. mycoides, and
B. subtilis. The developed microarray analysis of amplified genetic markers protocol provides an efficient method for (i) unambiguous identification and discrimination of
B. anthracis from other
Bacillus species and (ii) distinguishing between plasmid-containing and plasmid-free
Bacillus anthracis strains.</description><subject>Anthrax toxins</subject><subject>Bacillus anthracis</subject><subject>Bacillus anthracis - classification</subject><subject>Bacillus anthracis - genetics</subject><subject>Bacillus anthracis - pathogenicity</subject><subject>Bacillus cereus</subject><subject>Bacillus cereus - classification</subject><subject>Bacillus cereus - genetics</subject><subject>Bacillus cereus - pathogenicity</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>DNA Probes</subject><subject>DNA, Bacterial - analysis</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Infectious diseases</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Multiplex PCR</subject><subject>Oligonucleotide Array Sequence Analysis</subject><subject>Polymerase Chain Reaction - methods</subject><subject>pXO1</subject><subject>pXO2</subject><subject>Sensitivity and Specificity</subject><subject>Virulence factors</subject><issn>0732-8893</issn><issn>1879-0070</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkEtv1DAURi0EokPhL6AICXYJ1_ErZgeFQkWlbsra8uOGepRHsROk6a_H0xmp3dGVZet897s-hLyj0FCg8uO2CdH-HqNPs4tz0wLwBlgDVDwjG9opXQMoeE42oFhbd51mJ-RVzlsA2moOL8kJFS2XAsSG_LwIOC2xj94ucZ6qua--WB-HYc2VnZabVC65crtqXIcl3pZGrO6bbUp2V93sXIoh3t2HX5MXvR0yvjmep-TX-bfrsx_15dX3i7PPl7XnTC1116PiivdtkNKiVYw7DF44RqVw5dED6tZrJqQIAq2lSJVQ4JhyynHeslPy4TC3rPNnxbyYMWaPw2AnnNdspJRatAr-CxZzmmq5n_jpAJaP5ZywN7cpjjbtDAWzd2625rHzfZIbYKY4L-G3x5bVjRgeokfJBXh_BGz2duiTnYrUR5xWXdfJwn09cFjk_Y2YTPYRJ48hJvSLCXN8yj7_AK_Gp3g</recordid><startdate>20040701</startdate><enddate>20040701</enddate><creator>Volokhov, Dmitriy</creator><creator>Pomerantsev, Andrei</creator><creator>Kivovich, Violetta</creator><creator>Rasooly, Avraham</creator><creator>Chizhikov, Vladimir</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20040701</creationdate><title>Identification of Bacillus anthracis by multiprobe microarray hybridization</title><author>Volokhov, Dmitriy ; Pomerantsev, Andrei ; Kivovich, Violetta ; Rasooly, Avraham ; Chizhikov, Vladimir</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c437t-8fe7474f2d66aea734bedc5b3165b2d6c0e92c93565d5eaa1e17570b37b7b4423</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Anthrax toxins</topic><topic>Bacillus anthracis</topic><topic>Bacillus anthracis - classification</topic><topic>Bacillus anthracis - genetics</topic><topic>Bacillus anthracis - pathogenicity</topic><topic>Bacillus cereus</topic><topic>Bacillus cereus - classification</topic><topic>Bacillus cereus - genetics</topic><topic>Bacillus cereus - pathogenicity</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>DNA Probes</topic><topic>DNA, Bacterial - analysis</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Infectious diseases</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Multiplex PCR</topic><topic>Oligonucleotide Array Sequence Analysis</topic><topic>Polymerase Chain Reaction - methods</topic><topic>pXO1</topic><topic>pXO2</topic><topic>Sensitivity and Specificity</topic><topic>Virulence factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Volokhov, Dmitriy</creatorcontrib><creatorcontrib>Pomerantsev, Andrei</creatorcontrib><creatorcontrib>Kivovich, Violetta</creatorcontrib><creatorcontrib>Rasooly, Avraham</creatorcontrib><creatorcontrib>Chizhikov, Vladimir</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Diagnostic microbiology and infectious disease</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Volokhov, Dmitriy</au><au>Pomerantsev, Andrei</au><au>Kivovich, Violetta</au><au>Rasooly, Avraham</au><au>Chizhikov, Vladimir</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of Bacillus anthracis by multiprobe microarray hybridization</atitle><jtitle>Diagnostic microbiology and infectious disease</jtitle><addtitle>Diagn Microbiol Infect Dis</addtitle><date>2004-07-01</date><risdate>2004</risdate><volume>49</volume><issue>3</issue><spage>163</spage><epage>171</epage><pages>163-171</pages><issn>0732-8893</issn><eissn>1879-0070</eissn><coden>DMIDDZ</coden><abstract>We have developed a rapid assay based on microarray analysis of amplified genetic markers for reliable identification of
Bacillus anthracis and its discrimination from other closely related bacterial species of the
Bacillus cereus group. By combining polymerase chain reaction (PCR) amplification of six
B. anthracis-specific genes (plasmid-associated genes encoding virulence factors (
cyaA,
pagA,
lef, and
capA,
capB,
capC) and one chromosomal marker BA-5449) with analysis of amplicons by microarray hybridization, we were able to unambiguously identify and discriminate
B. anthracis among other closely related species.
Bacillus identification relied on hybridization with multiple individual microarray oligonucleotide probes (oligoprobes) specific to each target
B. anthracis gene. Evaluation of the assay was conducted using several
B. anthracis strains (with or without pXO1 and pXO2 plasmids) as well as over 50 other species phylogenetically related to
B. anthracis, including
B. cereus,
B. thuringiensis,
B. mycoides, and
B. subtilis. The developed microarray analysis of amplified genetic markers protocol provides an efficient method for (i) unambiguous identification and discrimination of
B. anthracis from other
Bacillus species and (ii) distinguishing between plasmid-containing and plasmid-free
Bacillus anthracis strains.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>15246505</pmid><doi>10.1016/j.diagmicrobio.2004.03.015</doi><tpages>9</tpages></addata></record> |
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| ispartof | Diagnostic microbiology and infectious disease, 2004-07, Vol.49 (3), p.163-171 |
| issn | 0732-8893 1879-0070 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Anthrax toxins Bacillus anthracis Bacillus anthracis - classification Bacillus anthracis - genetics Bacillus anthracis - pathogenicity Bacillus cereus Bacillus cereus - classification Bacillus cereus - genetics Bacillus cereus - pathogenicity Base Sequence Biological and medical sciences DNA Probes DNA, Bacterial - analysis Female Fundamental and applied biological sciences. Psychology Humans Infectious diseases Male Medical sciences Microbiology Molecular Sequence Data Multiplex PCR Oligonucleotide Array Sequence Analysis Polymerase Chain Reaction - methods pXO1 pXO2 Sensitivity and Specificity Virulence factors |
| title | Identification of Bacillus anthracis by multiprobe microarray hybridization |
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