Characterization of BRCAA1 and Its Novel Antigen Epitope Identification
Looking for novel breast cancer antigen epitopes is helpful for its treatment, diagnosis, and prevention. brcaa1 gene is mapped at 1q42.1-q43, its whole genome is 93.857 kb, including 18 exons and 17 introns. BRCAA1 protein is composed of 1,214 amino acids with 10 glycosylate sites, and shares 37% a...
Gespeichert in:
| Veröffentlicht in: | Cancer epidemiology, biomarkers & prevention biomarkers & prevention, 2004-07, Vol.13 (7), p.1136-1145 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1145 |
|---|---|
| container_issue | 7 |
| container_start_page | 1136 |
| container_title | Cancer epidemiology, biomarkers & prevention |
| container_volume | 13 |
| creator | Cui, Daxiang Jin, Guiqiu Gao, Tianwen Sun, Tianbai Tian, Furong Estrada, Giovani Gomez Gao, Huajian Sarai, Akinori |
| description | Looking for novel breast cancer antigen epitopes is helpful for its treatment, diagnosis, and prevention. brcaa1 gene is mapped at 1q42.1-q43, its whole genome is 93.857 kb, including 18 exons and 17 introns. BRCAA1 protein is composed
of 1,214 amino acids with 10 glycosylate sites, and shares 37% amino acid identity and an identical antigen epitope with Rb
binding protein 1. The novel antigen epitope, SSKKQKRSHK, was predicted to locate in the region 610 to 619 sites, was synthesized,
and its antibody was fabricated. Competent inhibition analysis showed that SSKKQKRSHK is the shortest effective peptide. The
antigen epitope was mapped in the cytoplasm of MCF-7 cells. Immunohistochemistry analysis showed that the antigen epitope
exhibited positive expression in 65% (39 of 60) breast cancer specimens and negative expression in 60 non-cancerous tissues.
Statistical analysis shows that its expression is closely associated with status of ER and PR, with sensitivity of 100% and
specificity of 81%, and confidence interval of 85.9% to 96.9%. ELISA analysis showed that the mean absorbance of sera antibody
titers from breast cancer patients and healthy donors were 0401 ± 0.163 SD and 0.137 ± 0.121 SD, respectively. Sixty-four
percent breast cancer patient sera and 13% healthy donor sera had higher titer than mean titer of healthy donors, and there
exists significant difference between breast cancer patients and healthy donors ( P < 0.001). In this study, a novel breast cancer antigen epitope, SSKKQKRSHK, is identified. Its expression is associated with
characteristics that are themselves associated with prognosis of breast cancer, and its sera antibody level may be helpful
for breast cancer diagnosis. |
| doi_str_mv | 10.1158/1055-9965.1136.13.7 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_66695216</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>17748090</sourcerecordid><originalsourceid>FETCH-LOGICAL-c506t-3bfa226b59360bb24195c410588fef04afe0967c4957286352dd437e5eed95d83</originalsourceid><addsrcrecordid>eNqFkF1r2zAUhsVoWbJuv2AwfNPSG2eS5SNZl2lIs0DZYGzXQpaPGg3HziSnpf31lZOUXvZKHzzv-XgI-crojDGovjMKkCslID25mDE-kx_IlAGvcikBztL9lZiQTzH-o5RKBfCRTBgUpWRFOSWrxcYEYwcM_tkMvu-y3mU3vxfzOctM12TrIWY_-wdss3k3-HvssuXOD_0Os3WD6cd5e4h9JufOtBG_nM4L8vd2-WfxI7_7tVov5ne5BSqGnNfOFIWoQXFB67oomQJbpjmryqGjpXFIlZC2VCCLSnAomqbkEgGxUdBU_IJcHevuQv9_j3HQWx8ttq3psN9HLYRQUDDxLsikLCuqaAL5EbShjzGg07vgtyY8aUb1KFqPGvWoUY-iNeNaptS3U_l9vcXmLXMym4DLE2CiNa0LprM-vnEibc6qcaHrI7fx95tHH1DbRGIIGNEEuzm0OzTmL_WukY8</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>17748090</pqid></control><display><type>article</type><title>Characterization of BRCAA1 and Its Novel Antigen Epitope Identification</title><source>MEDLINE</source><source>American Association for Cancer Research</source><source>EZB-FREE-00999 freely available EZB journals</source><creator>Cui, Daxiang ; Jin, Guiqiu ; Gao, Tianwen ; Sun, Tianbai ; Tian, Furong ; Estrada, Giovani Gomez ; Gao, Huajian ; Sarai, Akinori</creator><creatorcontrib>Cui, Daxiang ; Jin, Guiqiu ; Gao, Tianwen ; Sun, Tianbai ; Tian, Furong ; Estrada, Giovani Gomez ; Gao, Huajian ; Sarai, Akinori</creatorcontrib><description>Looking for novel breast cancer antigen epitopes is helpful for its treatment, diagnosis, and prevention. brcaa1 gene is mapped at 1q42.1-q43, its whole genome is 93.857 kb, including 18 exons and 17 introns. BRCAA1 protein is composed
of 1,214 amino acids with 10 glycosylate sites, and shares 37% amino acid identity and an identical antigen epitope with Rb
binding protein 1. The novel antigen epitope, SSKKQKRSHK, was predicted to locate in the region 610 to 619 sites, was synthesized,
and its antibody was fabricated. Competent inhibition analysis showed that SSKKQKRSHK is the shortest effective peptide. The
antigen epitope was mapped in the cytoplasm of MCF-7 cells. Immunohistochemistry analysis showed that the antigen epitope
exhibited positive expression in 65% (39 of 60) breast cancer specimens and negative expression in 60 non-cancerous tissues.
Statistical analysis shows that its expression is closely associated with status of ER and PR, with sensitivity of 100% and
specificity of 81%, and confidence interval of 85.9% to 96.9%. ELISA analysis showed that the mean absorbance of sera antibody
titers from breast cancer patients and healthy donors were 0401 ± 0.163 SD and 0.137 ± 0.121 SD, respectively. Sixty-four
percent breast cancer patient sera and 13% healthy donor sera had higher titer than mean titer of healthy donors, and there
exists significant difference between breast cancer patients and healthy donors ( P < 0.001). In this study, a novel breast cancer antigen epitope, SSKKQKRSHK, is identified. Its expression is associated with
characteristics that are themselves associated with prognosis of breast cancer, and its sera antibody level may be helpful
for breast cancer diagnosis.</description><identifier>ISSN: 1055-9965</identifier><identifier>EISSN: 1538-7755</identifier><identifier>DOI: 10.1158/1055-9965.1136.13.7</identifier><identifier>PMID: 15247124</identifier><language>eng</language><publisher>Philadelphia, PA: American Association for Cancer Research</publisher><subject>Adult ; Antigens, Neoplasm - genetics ; Antigens, Neoplasm - immunology ; Biological and medical sciences ; Biomarkers, Tumor - blood ; Blotting, Western ; Breast Neoplasms - blood ; Breast Neoplasms - genetics ; Breast Neoplasms - immunology ; Enzyme-Linked Immunosorbent Assay ; Epitope Mapping ; Female ; Fluorescence ; Humans ; Immunoglobulin G ; Medical sciences ; Middle Aged ; Neoplasm Proteins - genetics ; Neoplasm Proteins - immunology ; Oncogene Protein pp60(v-src) ; Peptide Fragments ; Polymerase Chain Reaction ; Prognosis ; Tumors</subject><ispartof>Cancer epidemiology, biomarkers & prevention, 2004-07, Vol.13 (7), p.1136-1145</ispartof><rights>2004 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c506t-3bfa226b59360bb24195c410588fef04afe0967c4957286352dd437e5eed95d83</citedby><cites>FETCH-LOGICAL-c506t-3bfa226b59360bb24195c410588fef04afe0967c4957286352dd437e5eed95d83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,3356,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16195188$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15247124$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cui, Daxiang</creatorcontrib><creatorcontrib>Jin, Guiqiu</creatorcontrib><creatorcontrib>Gao, Tianwen</creatorcontrib><creatorcontrib>Sun, Tianbai</creatorcontrib><creatorcontrib>Tian, Furong</creatorcontrib><creatorcontrib>Estrada, Giovani Gomez</creatorcontrib><creatorcontrib>Gao, Huajian</creatorcontrib><creatorcontrib>Sarai, Akinori</creatorcontrib><title>Characterization of BRCAA1 and Its Novel Antigen Epitope Identification</title><title>Cancer epidemiology, biomarkers & prevention</title><addtitle>Cancer Epidemiol Biomarkers Prev</addtitle><description>Looking for novel breast cancer antigen epitopes is helpful for its treatment, diagnosis, and prevention. brcaa1 gene is mapped at 1q42.1-q43, its whole genome is 93.857 kb, including 18 exons and 17 introns. BRCAA1 protein is composed
of 1,214 amino acids with 10 glycosylate sites, and shares 37% amino acid identity and an identical antigen epitope with Rb
binding protein 1. The novel antigen epitope, SSKKQKRSHK, was predicted to locate in the region 610 to 619 sites, was synthesized,
and its antibody was fabricated. Competent inhibition analysis showed that SSKKQKRSHK is the shortest effective peptide. The
antigen epitope was mapped in the cytoplasm of MCF-7 cells. Immunohistochemistry analysis showed that the antigen epitope
exhibited positive expression in 65% (39 of 60) breast cancer specimens and negative expression in 60 non-cancerous tissues.
Statistical analysis shows that its expression is closely associated with status of ER and PR, with sensitivity of 100% and
specificity of 81%, and confidence interval of 85.9% to 96.9%. ELISA analysis showed that the mean absorbance of sera antibody
titers from breast cancer patients and healthy donors were 0401 ± 0.163 SD and 0.137 ± 0.121 SD, respectively. Sixty-four
percent breast cancer patient sera and 13% healthy donor sera had higher titer than mean titer of healthy donors, and there
exists significant difference between breast cancer patients and healthy donors ( P < 0.001). In this study, a novel breast cancer antigen epitope, SSKKQKRSHK, is identified. Its expression is associated with
characteristics that are themselves associated with prognosis of breast cancer, and its sera antibody level may be helpful
for breast cancer diagnosis.</description><subject>Adult</subject><subject>Antigens, Neoplasm - genetics</subject><subject>Antigens, Neoplasm - immunology</subject><subject>Biological and medical sciences</subject><subject>Biomarkers, Tumor - blood</subject><subject>Blotting, Western</subject><subject>Breast Neoplasms - blood</subject><subject>Breast Neoplasms - genetics</subject><subject>Breast Neoplasms - immunology</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Epitope Mapping</subject><subject>Female</subject><subject>Fluorescence</subject><subject>Humans</subject><subject>Immunoglobulin G</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Neoplasm Proteins - genetics</subject><subject>Neoplasm Proteins - immunology</subject><subject>Oncogene Protein pp60(v-src)</subject><subject>Peptide Fragments</subject><subject>Polymerase Chain Reaction</subject><subject>Prognosis</subject><subject>Tumors</subject><issn>1055-9965</issn><issn>1538-7755</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkF1r2zAUhsVoWbJuv2AwfNPSG2eS5SNZl2lIs0DZYGzXQpaPGg3HziSnpf31lZOUXvZKHzzv-XgI-crojDGovjMKkCslID25mDE-kx_IlAGvcikBztL9lZiQTzH-o5RKBfCRTBgUpWRFOSWrxcYEYwcM_tkMvu-y3mU3vxfzOctM12TrIWY_-wdss3k3-HvssuXOD_0Os3WD6cd5e4h9JufOtBG_nM4L8vd2-WfxI7_7tVov5ne5BSqGnNfOFIWoQXFB67oomQJbpjmryqGjpXFIlZC2VCCLSnAomqbkEgGxUdBU_IJcHevuQv9_j3HQWx8ttq3psN9HLYRQUDDxLsikLCuqaAL5EbShjzGg07vgtyY8aUb1KFqPGvWoUY-iNeNaptS3U_l9vcXmLXMym4DLE2CiNa0LprM-vnEibc6qcaHrI7fx95tHH1DbRGIIGNEEuzm0OzTmL_WukY8</recordid><startdate>20040701</startdate><enddate>20040701</enddate><creator>Cui, Daxiang</creator><creator>Jin, Guiqiu</creator><creator>Gao, Tianwen</creator><creator>Sun, Tianbai</creator><creator>Tian, Furong</creator><creator>Estrada, Giovani Gomez</creator><creator>Gao, Huajian</creator><creator>Sarai, Akinori</creator><general>American Association for Cancer Research</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TO</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20040701</creationdate><title>Characterization of BRCAA1 and Its Novel Antigen Epitope Identification</title><author>Cui, Daxiang ; Jin, Guiqiu ; Gao, Tianwen ; Sun, Tianbai ; Tian, Furong ; Estrada, Giovani Gomez ; Gao, Huajian ; Sarai, Akinori</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c506t-3bfa226b59360bb24195c410588fef04afe0967c4957286352dd437e5eed95d83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Adult</topic><topic>Antigens, Neoplasm - genetics</topic><topic>Antigens, Neoplasm - immunology</topic><topic>Biological and medical sciences</topic><topic>Biomarkers, Tumor - blood</topic><topic>Blotting, Western</topic><topic>Breast Neoplasms - blood</topic><topic>Breast Neoplasms - genetics</topic><topic>Breast Neoplasms - immunology</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Epitope Mapping</topic><topic>Female</topic><topic>Fluorescence</topic><topic>Humans</topic><topic>Immunoglobulin G</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Neoplasm Proteins - genetics</topic><topic>Neoplasm Proteins - immunology</topic><topic>Oncogene Protein pp60(v-src)</topic><topic>Peptide Fragments</topic><topic>Polymerase Chain Reaction</topic><topic>Prognosis</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cui, Daxiang</creatorcontrib><creatorcontrib>Jin, Guiqiu</creatorcontrib><creatorcontrib>Gao, Tianwen</creatorcontrib><creatorcontrib>Sun, Tianbai</creatorcontrib><creatorcontrib>Tian, Furong</creatorcontrib><creatorcontrib>Estrada, Giovani Gomez</creatorcontrib><creatorcontrib>Gao, Huajian</creatorcontrib><creatorcontrib>Sarai, Akinori</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Cancer epidemiology, biomarkers & prevention</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cui, Daxiang</au><au>Jin, Guiqiu</au><au>Gao, Tianwen</au><au>Sun, Tianbai</au><au>Tian, Furong</au><au>Estrada, Giovani Gomez</au><au>Gao, Huajian</au><au>Sarai, Akinori</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of BRCAA1 and Its Novel Antigen Epitope Identification</atitle><jtitle>Cancer epidemiology, biomarkers & prevention</jtitle><addtitle>Cancer Epidemiol Biomarkers Prev</addtitle><date>2004-07-01</date><risdate>2004</risdate><volume>13</volume><issue>7</issue><spage>1136</spage><epage>1145</epage><pages>1136-1145</pages><issn>1055-9965</issn><eissn>1538-7755</eissn><abstract>Looking for novel breast cancer antigen epitopes is helpful for its treatment, diagnosis, and prevention. brcaa1 gene is mapped at 1q42.1-q43, its whole genome is 93.857 kb, including 18 exons and 17 introns. BRCAA1 protein is composed
of 1,214 amino acids with 10 glycosylate sites, and shares 37% amino acid identity and an identical antigen epitope with Rb
binding protein 1. The novel antigen epitope, SSKKQKRSHK, was predicted to locate in the region 610 to 619 sites, was synthesized,
and its antibody was fabricated. Competent inhibition analysis showed that SSKKQKRSHK is the shortest effective peptide. The
antigen epitope was mapped in the cytoplasm of MCF-7 cells. Immunohistochemistry analysis showed that the antigen epitope
exhibited positive expression in 65% (39 of 60) breast cancer specimens and negative expression in 60 non-cancerous tissues.
Statistical analysis shows that its expression is closely associated with status of ER and PR, with sensitivity of 100% and
specificity of 81%, and confidence interval of 85.9% to 96.9%. ELISA analysis showed that the mean absorbance of sera antibody
titers from breast cancer patients and healthy donors were 0401 ± 0.163 SD and 0.137 ± 0.121 SD, respectively. Sixty-four
percent breast cancer patient sera and 13% healthy donor sera had higher titer than mean titer of healthy donors, and there
exists significant difference between breast cancer patients and healthy donors ( P < 0.001). In this study, a novel breast cancer antigen epitope, SSKKQKRSHK, is identified. Its expression is associated with
characteristics that are themselves associated with prognosis of breast cancer, and its sera antibody level may be helpful
for breast cancer diagnosis.</abstract><cop>Philadelphia, PA</cop><pub>American Association for Cancer Research</pub><pmid>15247124</pmid><doi>10.1158/1055-9965.1136.13.7</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1055-9965 |
| ispartof | Cancer epidemiology, biomarkers & prevention, 2004-07, Vol.13 (7), p.1136-1145 |
| issn | 1055-9965 1538-7755 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_66695216 |
| source | MEDLINE; American Association for Cancer Research; EZB-FREE-00999 freely available EZB journals |
| subjects | Adult Antigens, Neoplasm - genetics Antigens, Neoplasm - immunology Biological and medical sciences Biomarkers, Tumor - blood Blotting, Western Breast Neoplasms - blood Breast Neoplasms - genetics Breast Neoplasms - immunology Enzyme-Linked Immunosorbent Assay Epitope Mapping Female Fluorescence Humans Immunoglobulin G Medical sciences Middle Aged Neoplasm Proteins - genetics Neoplasm Proteins - immunology Oncogene Protein pp60(v-src) Peptide Fragments Polymerase Chain Reaction Prognosis Tumors |
| title | Characterization of BRCAA1 and Its Novel Antigen Epitope Identification |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-05T03%3A32%3A59IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Characterization%20of%20BRCAA1%20and%20Its%20Novel%20Antigen%20Epitope%20Identification&rft.jtitle=Cancer%20epidemiology,%20biomarkers%20&%20prevention&rft.au=Cui,%20Daxiang&rft.date=2004-07-01&rft.volume=13&rft.issue=7&rft.spage=1136&rft.epage=1145&rft.pages=1136-1145&rft.issn=1055-9965&rft.eissn=1538-7755&rft_id=info:doi/10.1158/1055-9965.1136.13.7&rft_dat=%3Cproquest_cross%3E17748090%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=17748090&rft_id=info:pmid/15247124&rfr_iscdi=true |