Enzyme-linked immunosorbent assay to detect Lawsonia intracellularis in rabbits with proliferative enteropathy
Lawsonia intracellularis is an obligate intracellular pathogenic bacterium that causes proliferative enteropathy in domestic and experimental animals. Antiserum against synthetic peptides of the Lawsonia surface antigen (LsaA) well recognized L. intracellularis in infected ileum by immunohistochemis...
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| Veröffentlicht in: | Journal of Veterinary Medical Science 2004, Vol.66(6), pp.735-737 |
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| container_title | Journal of Veterinary Medical Science |
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| creator | Watarai, M. (Obihiro Univ. of Agriculture and Veterinary Medicine, Hokkaido (Japan)) Yamato, Y Horiuchi, N Kim, S Omata, Y Shirahata, T Furuoka, H |
| description | Lawsonia intracellularis is an obligate intracellular pathogenic bacterium that causes proliferative enteropathy in domestic and experimental animals. Antiserum against synthetic peptides of the Lawsonia surface antigen (LsaA) well recognized L. intracellularis in infected ileum by immunohistochemistry. The synthetic peptides in LsaA showed strong reaction with serum from rabbits infected with L. intracellularis by enzyme-linked immunosorbent assay. These results suggest that ELISA used synthetic peptides in LsaA and anti-LsaA serum might be useful to diagnose for proliferative enteropathy. |
| doi_str_mv | 10.1292/jvms.66.735 |
| format | Article |
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(Obihiro Univ. of Agriculture and Veterinary Medicine, Hokkaido (Japan)) ; Yamato, Y ; Horiuchi, N ; Kim, S ; Omata, Y ; Shirahata, T ; Furuoka, H</creator><creatorcontrib>Watarai, M. (Obihiro Univ. of Agriculture and Veterinary Medicine, Hokkaido (Japan)) ; Yamato, Y ; Horiuchi, N ; Kim, S ; Omata, Y ; Shirahata, T ; Furuoka, H</creatorcontrib><description>Lawsonia intracellularis is an obligate intracellular pathogenic bacterium that causes proliferative enteropathy in domestic and experimental animals. Antiserum against synthetic peptides of the Lawsonia surface antigen (LsaA) well recognized L. intracellularis in infected ileum by immunohistochemistry. The synthetic peptides in LsaA showed strong reaction with serum from rabbits infected with L. intracellularis by enzyme-linked immunosorbent assay. These results suggest that ELISA used synthetic peptides in LsaA and anti-LsaA serum might be useful to diagnose for proliferative enteropathy.</description><identifier>ISSN: 0916-7250</identifier><identifier>EISSN: 1347-7439</identifier><identifier>DOI: 10.1292/jvms.66.735</identifier><identifier>PMID: 15240954</identifier><language>eng</language><publisher>Japan: JAPANESE SOCIETY OF VETERINARY SCIENCE</publisher><subject>Adhesins, Bacterial - immunology ; Animals ; Antibodies, Bacterial - blood ; Antigens, Bacterial - immunology ; Antigens, Surface - immunology ; Cells, Cultured - microbiology ; Desulfovibrionaceae Infections - immunology ; Desulfovibrionaceae Infections - microbiology ; Desulfovibrionaceae Infections - veterinary ; DIAGNOSIS ; DIGESTIVE SYSTEM DISEASES ; ELISA ; Enzyme-Linked Immunosorbent Assay - veterinary ; Feces - microbiology ; Female ; Immunohistochemistry - veterinary ; Intestinal Diseases - immunology ; Intestinal Diseases - microbiology ; Intestinal Diseases - veterinary ; Lawsonia Bacteria - immunology ; Lawsonia Bacteria - isolation & purification ; LAWSONIA INTRACELLULARIS ; Microscopy, Fluorescence - veterinary ; rabbit ; RABBITS ; Rabbits - microbiology</subject><ispartof>Journal of Veterinary Medical Science, 2004, Vol.66(6), pp.735-737</ispartof><rights>2004 by the Japanese Society of Veterinary Science</rights><rights>Copyright Japan Science and Technology Agency 2004</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c550t-1539396cd176b333f17b38d8a6fa74ecb854856c8eb279e465953019dd06b183</citedby><cites>FETCH-LOGICAL-c550t-1539396cd176b333f17b38d8a6fa74ecb854856c8eb279e465953019dd06b183</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,1877,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15240954$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Watarai, M. (Obihiro Univ. of Agriculture and Veterinary Medicine, Hokkaido (Japan))</creatorcontrib><creatorcontrib>Yamato, Y</creatorcontrib><creatorcontrib>Horiuchi, N</creatorcontrib><creatorcontrib>Kim, S</creatorcontrib><creatorcontrib>Omata, Y</creatorcontrib><creatorcontrib>Shirahata, T</creatorcontrib><creatorcontrib>Furuoka, H</creatorcontrib><title>Enzyme-linked immunosorbent assay to detect Lawsonia intracellularis in rabbits with proliferative enteropathy</title><title>Journal of Veterinary Medical Science</title><addtitle>J. Vet. Med. Sci.</addtitle><description>Lawsonia intracellularis is an obligate intracellular pathogenic bacterium that causes proliferative enteropathy in domestic and experimental animals. Antiserum against synthetic peptides of the Lawsonia surface antigen (LsaA) well recognized L. intracellularis in infected ileum by immunohistochemistry. The synthetic peptides in LsaA showed strong reaction with serum from rabbits infected with L. intracellularis by enzyme-linked immunosorbent assay. These results suggest that ELISA used synthetic peptides in LsaA and anti-LsaA serum might be useful to diagnose for proliferative enteropathy.</description><subject>Adhesins, Bacterial - immunology</subject><subject>Animals</subject><subject>Antibodies, Bacterial - blood</subject><subject>Antigens, Bacterial - immunology</subject><subject>Antigens, Surface - immunology</subject><subject>Cells, Cultured - microbiology</subject><subject>Desulfovibrionaceae Infections - immunology</subject><subject>Desulfovibrionaceae Infections - microbiology</subject><subject>Desulfovibrionaceae Infections - veterinary</subject><subject>DIAGNOSIS</subject><subject>DIGESTIVE SYSTEM DISEASES</subject><subject>ELISA</subject><subject>Enzyme-Linked Immunosorbent Assay - veterinary</subject><subject>Feces - microbiology</subject><subject>Female</subject><subject>Immunohistochemistry - veterinary</subject><subject>Intestinal Diseases - immunology</subject><subject>Intestinal Diseases - microbiology</subject><subject>Intestinal Diseases - veterinary</subject><subject>Lawsonia Bacteria - immunology</subject><subject>Lawsonia Bacteria - isolation & purification</subject><subject>LAWSONIA INTRACELLULARIS</subject><subject>Microscopy, Fluorescence - veterinary</subject><subject>rabbit</subject><subject>RABBITS</subject><subject>Rabbits - microbiology</subject><issn>0916-7250</issn><issn>1347-7439</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc-L1DAUx4Mo7rh68qwEBC_SMWmaXzdl2V2VAT3sPaTt607GNhmTdJfxrzdDhxG8eEkI78Mn770vQq8pWdNa1x93D1NaC7GWjD9BK8oaWcmG6adoRTQVlaw5uUAvUtoRUtNG6OfogvK6IZo3K-Sv_e_DBNXo_E_osZum2YcUYgs-Y5uSPeAccA8Zuow39jEF7yx2PkfbwTjOo40ulTeOtm1dTvjR5S3exzC6AaLN7gFwUUEMe5u3h5fo2WDHBK9O9yW6u7m-u_pSbb7ffr36vKk6zkmuKGeaadH1VIqWMTZQ2TLVKysGKxvoWsUbxUWnoK2lhkZwzRmhuu-JaKlil-j9oi2N_JohZTO5dOzXeghzMkIIpYWk_wWpVFzVQhTw3T_gLszRlxlM2amShBJ5pD4sVBdDShEGs49usvFgKDHHsMwxrPK9KWEV-u3JObcT9H_ZUzoF-LQAu5TtPZwBG7PrRjjLxHIU57nUbW004IvizaIYbDD2voRlvv2oCeGEUMY5-wMQpbG8</recordid><startdate>20040601</startdate><enddate>20040601</enddate><creator>Watarai, M. 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| subjects | Adhesins, Bacterial - immunology Animals Antibodies, Bacterial - blood Antigens, Bacterial - immunology Antigens, Surface - immunology Cells, Cultured - microbiology Desulfovibrionaceae Infections - immunology Desulfovibrionaceae Infections - microbiology Desulfovibrionaceae Infections - veterinary DIAGNOSIS DIGESTIVE SYSTEM DISEASES ELISA Enzyme-Linked Immunosorbent Assay - veterinary Feces - microbiology Female Immunohistochemistry - veterinary Intestinal Diseases - immunology Intestinal Diseases - microbiology Intestinal Diseases - veterinary Lawsonia Bacteria - immunology Lawsonia Bacteria - isolation & purification LAWSONIA INTRACELLULARIS Microscopy, Fluorescence - veterinary rabbit RABBITS Rabbits - microbiology |
| title | Enzyme-linked immunosorbent assay to detect Lawsonia intracellularis in rabbits with proliferative enteropathy |
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