Ex vivo proton MR spectroscopy ( 1H-MRS) for evaluation of human gastric carcinoma
The present study was performed to determine the characteristics of the biochemical metabolites related to gastric cancer using ex vivo 1H magnetic resonance spectroscopy (MRS), and to assess the clinical usefulness. A total of 35 gastric specimens resected during surgery for gastric cancer were use...
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| Veröffentlicht in: | Magnetic resonance imaging 2004-07, Vol.22 (6), p.861-870 |
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| creator | Mun, Chi-Woong Cho, Ji-Youn Shin, Woon-Jae Choi, Ki-Seung Eun, Choong-Ki Cha, Seong Sook Lee, Junghee Yang, Young Nam, Sang-Hee Kim, Jeongkoo Lee, Soo Yeol |
| description | The present study was performed to determine the characteristics of the biochemical metabolites related to gastric cancer using ex vivo
1H magnetic resonance spectroscopy (MRS), and to assess the clinical usefulness. A total of 35 gastric specimens resected during surgery for gastric cancer were used to compare MR spectra. A 1.5-T (64-MHz) clinical MR imager equipped with facilities for spectroscopy was used to obtain MR spectra from 33 gastric specimens. High-resolution
1H nuclear magnetic resonance (NMR) spectra of the remains of two specimens were also examined with a 9.4-T (400-MHz) NMR spectrometer. Localized spectroscopic measurements were performed in two layers of gastric tissue, the proper muscle layer and the composite mucosa/submucosa layer. T
2 FSE and 3D SPGR images were used to determine the voxel size and the location for MRS data collection. MR spectra were obtained using the single-voxel PRESS technique with parameters of TR/TE = 2000/30 ms, NA = 256, and voxel size = 3 × 3 × 3 mm
3 (27 μL). Cancerous and noncancerous gastric tissues in the voxel were determined by histopathological analysis. On 9.4-T ex vivo NMR spectroscopy, the following metabolite peaks were found: lipids at 0.9 ppm (CH
3) and 1.3 ppm (CH
2); alanine (β-CH
3) at 1.58 ppm; N-Acetyl neuraminic acid (NANA: sialic acid) at 2.03 ppm; and glutathione at 2.25 ppm in normal gastric tissue layers. In the 1.5-T MR system, broad and featureless spectral peaks of the various metabolites in normal human gastric tissue were observed at 0.9 ppm, 1.3 ppm, 2.0 ppm, and 2.2 ppm regardless of gastric tissue layer. In specimens (Borrmann type III) with tubular adenocarcinoma, resonance peaks were observed at 1.26 ppm, 1.36 ppm (doublet of lactate), and 3.22 ppm (choline). Cancer lesions showed decreased levels of lipid peaks, showing the significant lactate doublet peaks, and increased intensity of the choline peak as compared with noncancerous gastric tissue. We found that decreased levels of lipids and increases in lactate and choline peaks in gastric tissue were markers for malignancy in gastric lesions. Information provided by ex vivo
1H MRS, together with the development of in vivo
1H MRS with high field strength and high resolution, may be very useful for the diagnosis of gastric cancer in clinical situation. |
| doi_str_mv | 10.1016/j.mri.2004.01.045 |
| format | Article |
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1H magnetic resonance spectroscopy (MRS), and to assess the clinical usefulness. A total of 35 gastric specimens resected during surgery for gastric cancer were used to compare MR spectra. A 1.5-T (64-MHz) clinical MR imager equipped with facilities for spectroscopy was used to obtain MR spectra from 33 gastric specimens. High-resolution
1H nuclear magnetic resonance (NMR) spectra of the remains of two specimens were also examined with a 9.4-T (400-MHz) NMR spectrometer. Localized spectroscopic measurements were performed in two layers of gastric tissue, the proper muscle layer and the composite mucosa/submucosa layer. T
2 FSE and 3D SPGR images were used to determine the voxel size and the location for MRS data collection. MR spectra were obtained using the single-voxel PRESS technique with parameters of TR/TE = 2000/30 ms, NA = 256, and voxel size = 3 × 3 × 3 mm
3 (27 μL). Cancerous and noncancerous gastric tissues in the voxel were determined by histopathological analysis. On 9.4-T ex vivo NMR spectroscopy, the following metabolite peaks were found: lipids at 0.9 ppm (CH
3) and 1.3 ppm (CH
2); alanine (β-CH
3) at 1.58 ppm; N-Acetyl neuraminic acid (NANA: sialic acid) at 2.03 ppm; and glutathione at 2.25 ppm in normal gastric tissue layers. In the 1.5-T MR system, broad and featureless spectral peaks of the various metabolites in normal human gastric tissue were observed at 0.9 ppm, 1.3 ppm, 2.0 ppm, and 2.2 ppm regardless of gastric tissue layer. In specimens (Borrmann type III) with tubular adenocarcinoma, resonance peaks were observed at 1.26 ppm, 1.36 ppm (doublet of lactate), and 3.22 ppm (choline). Cancer lesions showed decreased levels of lipid peaks, showing the significant lactate doublet peaks, and increased intensity of the choline peak as compared with noncancerous gastric tissue. We found that decreased levels of lipids and increases in lactate and choline peaks in gastric tissue were markers for malignancy in gastric lesions. Information provided by ex vivo
1H MRS, together with the development of in vivo
1H MRS with high field strength and high resolution, may be very useful for the diagnosis of gastric cancer in clinical situation.</description><identifier>ISSN: 0730-725X</identifier><identifier>EISSN: 1873-5894</identifier><identifier>DOI: 10.1016/j.mri.2004.01.045</identifier><identifier>PMID: 15234456</identifier><language>eng</language><publisher>Netherlands: Elsevier Inc</publisher><subject>Adenocarcinoma - diagnosis ; Adenocarcinoma - metabolism ; Adenocarcinoma - pathology ; Alanine - metabolism ; Cancer ; Choline - metabolism ; Feasibility Studies ; Gastric tissue ; Humans ; Lipid Metabolism ; Magnetic Resonance Spectroscopy ; Magnetic resonance spectroscopy (MRS) ; N-Acetylneuraminic Acid - metabolism ; Neoplasm Invasiveness ; Protons ; Specimens ; Stomach ; Stomach Neoplasms - diagnosis ; Stomach Neoplasms - metabolism ; Stomach Neoplasms - pathology</subject><ispartof>Magnetic resonance imaging, 2004-07, Vol.22 (6), p.861-870</ispartof><rights>2004 Elsevier Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c349t-dcd86da1c7b473f9c4d546650e2cb0ad1c1f065317d6f739a14460a30fab32a83</citedby><cites>FETCH-LOGICAL-c349t-dcd86da1c7b473f9c4d546650e2cb0ad1c1f065317d6f739a14460a30fab32a83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0730725X04000645$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15234456$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mun, Chi-Woong</creatorcontrib><creatorcontrib>Cho, Ji-Youn</creatorcontrib><creatorcontrib>Shin, Woon-Jae</creatorcontrib><creatorcontrib>Choi, Ki-Seung</creatorcontrib><creatorcontrib>Eun, Choong-Ki</creatorcontrib><creatorcontrib>Cha, Seong Sook</creatorcontrib><creatorcontrib>Lee, Junghee</creatorcontrib><creatorcontrib>Yang, Young</creatorcontrib><creatorcontrib>Nam, Sang-Hee</creatorcontrib><creatorcontrib>Kim, Jeongkoo</creatorcontrib><creatorcontrib>Lee, Soo Yeol</creatorcontrib><title>Ex vivo proton MR spectroscopy ( 1H-MRS) for evaluation of human gastric carcinoma</title><title>Magnetic resonance imaging</title><addtitle>Magn Reson Imaging</addtitle><description>The present study was performed to determine the characteristics of the biochemical metabolites related to gastric cancer using ex vivo
1H magnetic resonance spectroscopy (MRS), and to assess the clinical usefulness. A total of 35 gastric specimens resected during surgery for gastric cancer were used to compare MR spectra. A 1.5-T (64-MHz) clinical MR imager equipped with facilities for spectroscopy was used to obtain MR spectra from 33 gastric specimens. High-resolution
1H nuclear magnetic resonance (NMR) spectra of the remains of two specimens were also examined with a 9.4-T (400-MHz) NMR spectrometer. Localized spectroscopic measurements were performed in two layers of gastric tissue, the proper muscle layer and the composite mucosa/submucosa layer. T
2 FSE and 3D SPGR images were used to determine the voxel size and the location for MRS data collection. MR spectra were obtained using the single-voxel PRESS technique with parameters of TR/TE = 2000/30 ms, NA = 256, and voxel size = 3 × 3 × 3 mm
3 (27 μL). Cancerous and noncancerous gastric tissues in the voxel were determined by histopathological analysis. On 9.4-T ex vivo NMR spectroscopy, the following metabolite peaks were found: lipids at 0.9 ppm (CH
3) and 1.3 ppm (CH
2); alanine (β-CH
3) at 1.58 ppm; N-Acetyl neuraminic acid (NANA: sialic acid) at 2.03 ppm; and glutathione at 2.25 ppm in normal gastric tissue layers. In the 1.5-T MR system, broad and featureless spectral peaks of the various metabolites in normal human gastric tissue were observed at 0.9 ppm, 1.3 ppm, 2.0 ppm, and 2.2 ppm regardless of gastric tissue layer. In specimens (Borrmann type III) with tubular adenocarcinoma, resonance peaks were observed at 1.26 ppm, 1.36 ppm (doublet of lactate), and 3.22 ppm (choline). Cancer lesions showed decreased levels of lipid peaks, showing the significant lactate doublet peaks, and increased intensity of the choline peak as compared with noncancerous gastric tissue. We found that decreased levels of lipids and increases in lactate and choline peaks in gastric tissue were markers for malignancy in gastric lesions. Information provided by ex vivo
1H MRS, together with the development of in vivo
1H MRS with high field strength and high resolution, may be very useful for the diagnosis of gastric cancer in clinical situation.</description><subject>Adenocarcinoma - diagnosis</subject><subject>Adenocarcinoma - metabolism</subject><subject>Adenocarcinoma - pathology</subject><subject>Alanine - metabolism</subject><subject>Cancer</subject><subject>Choline - metabolism</subject><subject>Feasibility Studies</subject><subject>Gastric tissue</subject><subject>Humans</subject><subject>Lipid Metabolism</subject><subject>Magnetic Resonance Spectroscopy</subject><subject>Magnetic resonance spectroscopy (MRS)</subject><subject>N-Acetylneuraminic Acid - metabolism</subject><subject>Neoplasm Invasiveness</subject><subject>Protons</subject><subject>Specimens</subject><subject>Stomach</subject><subject>Stomach Neoplasms - diagnosis</subject><subject>Stomach Neoplasms - metabolism</subject><subject>Stomach Neoplasms - pathology</subject><issn>0730-725X</issn><issn>1873-5894</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEtLAzEUhYMoWh8_wI1kJbqY8WbymBlcSfEFLUJVcBfSJKMpnUlNZor-eyMtuHN1Nt893PMhdEogJ0DE1SJvg8sLAJYDyYHxHTQiVUkzXtVsF42gpJCVBX87QIcxLgCAF5TvowOSkjEuRmh2-4XXbu3xKvjed3g6w3FldR981H71jS8wecims-dL3PiA7VotB9W7BPoGfwyt6vC7in1wGmsVtOt8q47RXqOW0Z5s8wi93t2-jB-yydP94_hmkmnK6j4z2lTCKKLLOStpU2tmOBOCgy30HJQhmjQgOCWlEU1Ja0UYE6AoNGpOC1XRI3S-6U2vfw429rJ1UdvlUnXWD1EKISqoaJ1AsgF1WhWDbeQquFaFb0lA_oqUC5lEyl-REohMItPN2bZ8mLfW_F1szSXgegPYNHHtbJBRO9tpa1xI_qTx7p_6H6OTgsI</recordid><startdate>20040701</startdate><enddate>20040701</enddate><creator>Mun, Chi-Woong</creator><creator>Cho, Ji-Youn</creator><creator>Shin, Woon-Jae</creator><creator>Choi, Ki-Seung</creator><creator>Eun, Choong-Ki</creator><creator>Cha, Seong Sook</creator><creator>Lee, Junghee</creator><creator>Yang, Young</creator><creator>Nam, Sang-Hee</creator><creator>Kim, Jeongkoo</creator><creator>Lee, Soo Yeol</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20040701</creationdate><title>Ex vivo proton MR spectroscopy ( 1H-MRS) for evaluation of human gastric carcinoma</title><author>Mun, Chi-Woong ; Cho, Ji-Youn ; Shin, Woon-Jae ; Choi, Ki-Seung ; Eun, Choong-Ki ; Cha, Seong Sook ; Lee, Junghee ; Yang, Young ; Nam, Sang-Hee ; Kim, Jeongkoo ; Lee, Soo Yeol</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c349t-dcd86da1c7b473f9c4d546650e2cb0ad1c1f065317d6f739a14460a30fab32a83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Adenocarcinoma - diagnosis</topic><topic>Adenocarcinoma - metabolism</topic><topic>Adenocarcinoma - pathology</topic><topic>Alanine - metabolism</topic><topic>Cancer</topic><topic>Choline - metabolism</topic><topic>Feasibility Studies</topic><topic>Gastric tissue</topic><topic>Humans</topic><topic>Lipid Metabolism</topic><topic>Magnetic Resonance Spectroscopy</topic><topic>Magnetic resonance spectroscopy (MRS)</topic><topic>N-Acetylneuraminic Acid - metabolism</topic><topic>Neoplasm Invasiveness</topic><topic>Protons</topic><topic>Specimens</topic><topic>Stomach</topic><topic>Stomach Neoplasms - diagnosis</topic><topic>Stomach Neoplasms - metabolism</topic><topic>Stomach Neoplasms - pathology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mun, Chi-Woong</creatorcontrib><creatorcontrib>Cho, Ji-Youn</creatorcontrib><creatorcontrib>Shin, Woon-Jae</creatorcontrib><creatorcontrib>Choi, Ki-Seung</creatorcontrib><creatorcontrib>Eun, Choong-Ki</creatorcontrib><creatorcontrib>Cha, Seong Sook</creatorcontrib><creatorcontrib>Lee, Junghee</creatorcontrib><creatorcontrib>Yang, Young</creatorcontrib><creatorcontrib>Nam, Sang-Hee</creatorcontrib><creatorcontrib>Kim, Jeongkoo</creatorcontrib><creatorcontrib>Lee, Soo Yeol</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Magnetic resonance imaging</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mun, Chi-Woong</au><au>Cho, Ji-Youn</au><au>Shin, Woon-Jae</au><au>Choi, Ki-Seung</au><au>Eun, Choong-Ki</au><au>Cha, Seong Sook</au><au>Lee, Junghee</au><au>Yang, Young</au><au>Nam, Sang-Hee</au><au>Kim, Jeongkoo</au><au>Lee, Soo Yeol</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Ex vivo proton MR spectroscopy ( 1H-MRS) for evaluation of human gastric carcinoma</atitle><jtitle>Magnetic resonance imaging</jtitle><addtitle>Magn Reson Imaging</addtitle><date>2004-07-01</date><risdate>2004</risdate><volume>22</volume><issue>6</issue><spage>861</spage><epage>870</epage><pages>861-870</pages><issn>0730-725X</issn><eissn>1873-5894</eissn><abstract>The present study was performed to determine the characteristics of the biochemical metabolites related to gastric cancer using ex vivo
1H magnetic resonance spectroscopy (MRS), and to assess the clinical usefulness. A total of 35 gastric specimens resected during surgery for gastric cancer were used to compare MR spectra. A 1.5-T (64-MHz) clinical MR imager equipped with facilities for spectroscopy was used to obtain MR spectra from 33 gastric specimens. High-resolution
1H nuclear magnetic resonance (NMR) spectra of the remains of two specimens were also examined with a 9.4-T (400-MHz) NMR spectrometer. Localized spectroscopic measurements were performed in two layers of gastric tissue, the proper muscle layer and the composite mucosa/submucosa layer. T
2 FSE and 3D SPGR images were used to determine the voxel size and the location for MRS data collection. MR spectra were obtained using the single-voxel PRESS technique with parameters of TR/TE = 2000/30 ms, NA = 256, and voxel size = 3 × 3 × 3 mm
3 (27 μL). Cancerous and noncancerous gastric tissues in the voxel were determined by histopathological analysis. On 9.4-T ex vivo NMR spectroscopy, the following metabolite peaks were found: lipids at 0.9 ppm (CH
3) and 1.3 ppm (CH
2); alanine (β-CH
3) at 1.58 ppm; N-Acetyl neuraminic acid (NANA: sialic acid) at 2.03 ppm; and glutathione at 2.25 ppm in normal gastric tissue layers. In the 1.5-T MR system, broad and featureless spectral peaks of the various metabolites in normal human gastric tissue were observed at 0.9 ppm, 1.3 ppm, 2.0 ppm, and 2.2 ppm regardless of gastric tissue layer. In specimens (Borrmann type III) with tubular adenocarcinoma, resonance peaks were observed at 1.26 ppm, 1.36 ppm (doublet of lactate), and 3.22 ppm (choline). Cancer lesions showed decreased levels of lipid peaks, showing the significant lactate doublet peaks, and increased intensity of the choline peak as compared with noncancerous gastric tissue. We found that decreased levels of lipids and increases in lactate and choline peaks in gastric tissue were markers for malignancy in gastric lesions. Information provided by ex vivo
1H MRS, together with the development of in vivo
1H MRS with high field strength and high resolution, may be very useful for the diagnosis of gastric cancer in clinical situation.</abstract><cop>Netherlands</cop><pub>Elsevier Inc</pub><pmid>15234456</pmid><doi>10.1016/j.mri.2004.01.045</doi><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0730-725X |
| ispartof | Magnetic resonance imaging, 2004-07, Vol.22 (6), p.861-870 |
| issn | 0730-725X 1873-5894 |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Adenocarcinoma - diagnosis Adenocarcinoma - metabolism Adenocarcinoma - pathology Alanine - metabolism Cancer Choline - metabolism Feasibility Studies Gastric tissue Humans Lipid Metabolism Magnetic Resonance Spectroscopy Magnetic resonance spectroscopy (MRS) N-Acetylneuraminic Acid - metabolism Neoplasm Invasiveness Protons Specimens Stomach Stomach Neoplasms - diagnosis Stomach Neoplasms - metabolism Stomach Neoplasms - pathology |
| title | Ex vivo proton MR spectroscopy ( 1H-MRS) for evaluation of human gastric carcinoma |
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