Application of microdialysis to cancellous bone tissue for measurement of gentamicin levels
Objectives: Knowledge concerning the distribution of antibiotics in bone tissue is valuable for pharmacokinetic and clinical use. Unfortunately, appropriate techniques are difficult to apply. We introduced microdialysis catheters to cancellous bone tissue for the investigation of gentamicin levels a...
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| Veröffentlicht in: | Journal of antimicrobial chemotherapy 2004-07, Vol.54 (1), p.263-265 |
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| container_title | Journal of antimicrobial chemotherapy |
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| creator | Stolle, Lars B. Arpi, Magnus Holmberg-Jørgensen, Peter Riegels-Nielsen, Per Keller, Johnny |
| description | Objectives: Knowledge concerning the distribution of antibiotics in bone tissue is valuable for pharmacokinetic and clinical use. Unfortunately, appropriate techniques are difficult to apply. We introduced microdialysis catheters to cancellous bone tissue for the investigation of gentamicin levels and compared the pharmacokinetics measured with values obtained from bone samples. Methods: After two microdialysis catheters had been inserted into cancellous bone, eight pigs received an intravenous bolus of 240 mg of gentamicin. Microdialysates and bone samples were obtained over a period of 6 h and drug concentrations were measured. Results: The area under the curves of the two microdialysates and bone samples were 1569, 1721 and 1533 mg·min/L (ANOVA, P=0.81). Reproducibility of the measurements from the microdialysates was defined as the mean ratio of AUC6/catheter no. 1/AUC6/catheter no. 2. This ratio was 1.02. Conclusions: Microdialysis is a suitable, relatively non-invasive and reproducible technique for dynamic and quantitative measurement of gentamicin levels in experimental research. |
| doi_str_mv | 10.1093/jac/dkh291 |
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Unfortunately, appropriate techniques are difficult to apply. We introduced microdialysis catheters to cancellous bone tissue for the investigation of gentamicin levels and compared the pharmacokinetics measured with values obtained from bone samples. Methods: After two microdialysis catheters had been inserted into cancellous bone, eight pigs received an intravenous bolus of 240 mg of gentamicin. Microdialysates and bone samples were obtained over a period of 6 h and drug concentrations were measured. Results: The area under the curves of the two microdialysates and bone samples were 1569, 1721 and 1533 mg·min/L (ANOVA, P=0.81). Reproducibility of the measurements from the microdialysates was defined as the mean ratio of AUC6/catheter no. 1/AUC6/catheter no. 2. This ratio was 1.02. Conclusions: Microdialysis is a suitable, relatively non-invasive and reproducible technique for dynamic and quantitative measurement of gentamicin levels in experimental research.</description><identifier>ISSN: 0305-7453</identifier><identifier>ISSN: 1460-2091</identifier><identifier>EISSN: 1460-2091</identifier><identifier>DOI: 10.1093/jac/dkh291</identifier><identifier>PMID: 15190036</identifier><identifier>CODEN: JACHDX</identifier><language>eng</language><publisher>Oxford: Oxford University Press</publisher><subject>Animals ; Anti-Bacterial Agents - analysis ; Anti-Bacterial Agents - blood ; Anti-Bacterial Agents - pharmacokinetics ; Antibiotics. Antiinfectious agents. Antiparasitic agents ; Area Under Curve ; bioassays ; Biological and medical sciences ; Bone and Bones - chemistry ; bone samples ; Calibration ; experimental research ; Female ; Gentamicins - analysis ; Gentamicins - blood ; Gentamicins - pharmacokinetics ; Medical sciences ; Microdialysis - methods ; pharmacokinetics ; Pharmacology. Drug treatments ; Reproducibility of Results ; Swine</subject><ispartof>Journal of antimicrobial chemotherapy, 2004-07, Vol.54 (1), p.263-265</ispartof><rights>2004 INIST-CNRS</rights><rights>Copyright Oxford University Press(England) Jul 2004</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c445t-af25a5954a1ff82a54f6990fe896b63234590187b4678e9332c16f386f47206b3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15975343$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15190036$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Stolle, Lars B.</creatorcontrib><creatorcontrib>Arpi, Magnus</creatorcontrib><creatorcontrib>Holmberg-Jørgensen, Peter</creatorcontrib><creatorcontrib>Riegels-Nielsen, Per</creatorcontrib><creatorcontrib>Keller, Johnny</creatorcontrib><title>Application of microdialysis to cancellous bone tissue for measurement of gentamicin levels</title><title>Journal of antimicrobial chemotherapy</title><addtitle>J. Antimicrob. Chemother</addtitle><description>Objectives: Knowledge concerning the distribution of antibiotics in bone tissue is valuable for pharmacokinetic and clinical use. Unfortunately, appropriate techniques are difficult to apply. We introduced microdialysis catheters to cancellous bone tissue for the investigation of gentamicin levels and compared the pharmacokinetics measured with values obtained from bone samples. Methods: After two microdialysis catheters had been inserted into cancellous bone, eight pigs received an intravenous bolus of 240 mg of gentamicin. Microdialysates and bone samples were obtained over a period of 6 h and drug concentrations were measured. Results: The area under the curves of the two microdialysates and bone samples were 1569, 1721 and 1533 mg·min/L (ANOVA, P=0.81). Reproducibility of the measurements from the microdialysates was defined as the mean ratio of AUC6/catheter no. 1/AUC6/catheter no. 2. This ratio was 1.02. Conclusions: Microdialysis is a suitable, relatively non-invasive and reproducible technique for dynamic and quantitative measurement of gentamicin levels in experimental research.</description><subject>Animals</subject><subject>Anti-Bacterial Agents - analysis</subject><subject>Anti-Bacterial Agents - blood</subject><subject>Anti-Bacterial Agents - pharmacokinetics</subject><subject>Antibiotics. Antiinfectious agents. Antiparasitic agents</subject><subject>Area Under Curve</subject><subject>bioassays</subject><subject>Biological and medical sciences</subject><subject>Bone and Bones - chemistry</subject><subject>bone samples</subject><subject>Calibration</subject><subject>experimental research</subject><subject>Female</subject><subject>Gentamicins - analysis</subject><subject>Gentamicins - blood</subject><subject>Gentamicins - pharmacokinetics</subject><subject>Medical sciences</subject><subject>Microdialysis - methods</subject><subject>pharmacokinetics</subject><subject>Pharmacology. Drug treatments</subject><subject>Reproducibility of Results</subject><subject>Swine</subject><issn>0305-7453</issn><issn>1460-2091</issn><issn>1460-2091</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUtr3TAQhUVpaW4em_6AIgrpouBGb1nLEPJoSAmBFkK6ELKvlOjGtm40dkn-fWXupS3ddDWL-ebMzDkIvaPkMyWGH61ce7R8fGCGvkILKhSpGDH0NVoQTmSlheQ7aBdgRQhRUtVv0Q6V1BDC1QL9OF6vu9i6MaYBp4D72Oa0jK57gQh4TLh1Q-u7Lk2AmzR4PEaAyeOQMu69gyn73g_jPHpfqivzccCd_-k72EdvguvAH2zrHvp-dvrt5KK6uj7_cnJ8VbVCyLFygUknjRSOhlAzJ0VQxpDga6MaxRkX0hBa60YoXXvDOWupCrxWQWhGVMP30MeN7jqnp8nDaPsI89Fu8OVuq5SqZyv-CzIimRbaFPDDP-AqTXkoT1hGdZHjNS_Qpw1UHAPIPth1jr3LL5YSOwdjSzB2E0yB328Vp6b3yz_oNokCHG4BB63rQi6-R_iLM1pyMW-tNlyE0T__7rv8aJXmWtqL2zt7-fVOnN-cXdob_gvXeKUb</recordid><startdate>20040701</startdate><enddate>20040701</enddate><creator>Stolle, Lars B.</creator><creator>Arpi, Magnus</creator><creator>Holmberg-Jørgensen, Peter</creator><creator>Riegels-Nielsen, Per</creator><creator>Keller, Johnny</creator><general>Oxford University Press</general><general>Oxford Publishing Limited (England)</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7U7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>NAPCQ</scope><scope>P64</scope><scope>7QP</scope><scope>7X8</scope></search><sort><creationdate>20040701</creationdate><title>Application of microdialysis to cancellous bone tissue for measurement of gentamicin levels</title><author>Stolle, Lars B. ; Arpi, Magnus ; Holmberg-Jørgensen, Peter ; Riegels-Nielsen, Per ; Keller, Johnny</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c445t-af25a5954a1ff82a54f6990fe896b63234590187b4678e9332c16f386f47206b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Animals</topic><topic>Anti-Bacterial Agents - analysis</topic><topic>Anti-Bacterial Agents - blood</topic><topic>Anti-Bacterial Agents - pharmacokinetics</topic><topic>Antibiotics. Antiinfectious agents. Antiparasitic agents</topic><topic>Area Under Curve</topic><topic>bioassays</topic><topic>Biological and medical sciences</topic><topic>Bone and Bones - chemistry</topic><topic>bone samples</topic><topic>Calibration</topic><topic>experimental research</topic><topic>Female</topic><topic>Gentamicins - analysis</topic><topic>Gentamicins - blood</topic><topic>Gentamicins - pharmacokinetics</topic><topic>Medical sciences</topic><topic>Microdialysis - methods</topic><topic>pharmacokinetics</topic><topic>Pharmacology. Drug treatments</topic><topic>Reproducibility of Results</topic><topic>Swine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Stolle, Lars B.</creatorcontrib><creatorcontrib>Arpi, Magnus</creatorcontrib><creatorcontrib>Holmberg-Jørgensen, Peter</creatorcontrib><creatorcontrib>Riegels-Nielsen, Per</creatorcontrib><creatorcontrib>Keller, Johnny</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Nursing & Allied Health Premium</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of antimicrobial chemotherapy</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Stolle, Lars B.</au><au>Arpi, Magnus</au><au>Holmberg-Jørgensen, Peter</au><au>Riegels-Nielsen, Per</au><au>Keller, Johnny</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Application of microdialysis to cancellous bone tissue for measurement of gentamicin levels</atitle><jtitle>Journal of antimicrobial chemotherapy</jtitle><addtitle>J. Antimicrob. Chemother</addtitle><date>2004-07-01</date><risdate>2004</risdate><volume>54</volume><issue>1</issue><spage>263</spage><epage>265</epage><pages>263-265</pages><issn>0305-7453</issn><issn>1460-2091</issn><eissn>1460-2091</eissn><coden>JACHDX</coden><abstract>Objectives: Knowledge concerning the distribution of antibiotics in bone tissue is valuable for pharmacokinetic and clinical use. Unfortunately, appropriate techniques are difficult to apply. We introduced microdialysis catheters to cancellous bone tissue for the investigation of gentamicin levels and compared the pharmacokinetics measured with values obtained from bone samples. Methods: After two microdialysis catheters had been inserted into cancellous bone, eight pigs received an intravenous bolus of 240 mg of gentamicin. Microdialysates and bone samples were obtained over a period of 6 h and drug concentrations were measured. Results: The area under the curves of the two microdialysates and bone samples were 1569, 1721 and 1533 mg·min/L (ANOVA, P=0.81). Reproducibility of the measurements from the microdialysates was defined as the mean ratio of AUC6/catheter no. 1/AUC6/catheter no. 2. This ratio was 1.02. Conclusions: Microdialysis is a suitable, relatively non-invasive and reproducible technique for dynamic and quantitative measurement of gentamicin levels in experimental research.</abstract><cop>Oxford</cop><pub>Oxford University Press</pub><pmid>15190036</pmid><doi>10.1093/jac/dkh291</doi><tpages>3</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Anti-Bacterial Agents - analysis Anti-Bacterial Agents - blood Anti-Bacterial Agents - pharmacokinetics Antibiotics. Antiinfectious agents. Antiparasitic agents Area Under Curve bioassays Biological and medical sciences Bone and Bones - chemistry bone samples Calibration experimental research Female Gentamicins - analysis Gentamicins - blood Gentamicins - pharmacokinetics Medical sciences Microdialysis - methods pharmacokinetics Pharmacology. Drug treatments Reproducibility of Results Swine |
| title | Application of microdialysis to cancellous bone tissue for measurement of gentamicin levels |
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