Molecular analysis of rugosity in a Vibrio cholerae O1 El Tor phase variant
Summary Reversible phase variation between the rugose and smooth colony variants is predicted to be important for the survival of Vibrio cholerae in natural aquatic habitats. Microarray expression profiling studies of the rugose and smooth variants of the same strain led to the identificatio...
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| Veröffentlicht in: | Molecular microbiology 2004-07, Vol.53 (2), p.497-515 |
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| creator | Yildiz, Fitnat H. Liu, Xiaole S. Heydorn, Arne Schoolnik, Gary K. |
| description | Summary
Reversible phase variation between the rugose and smooth colony variants is predicted to be important for the survival of Vibrio cholerae in natural aquatic habitats. Microarray expression profiling studies of the rugose and smooth variants of the same strain led to the identification of 124 differentially regulated genes. Further expression profiling experiments showed how these genes are regulated by the VpsR and HapR transcription factors, which, respectively, positively and negatively regulate production of VPSEl Tor, a rugose‐associated extracellular polysaccharide. The study of mutants of rpoN and rpoS demonstrated the effects of these alternative sigma factors on phase variation‐specific gene expression. Bioinformatics analysis of these expression data shows that ‘rugosity’ and ‘smoothness’ are determined by a complex hierarchy of positive and negative regulators, which also affect the biofilm, surface hydrophobicity and motility phenotypes of the organism. |
| doi_str_mv | 10.1111/j.1365-2958.2004.04154.x |
| format | Article |
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Reversible phase variation between the rugose and smooth colony variants is predicted to be important for the survival of Vibrio cholerae in natural aquatic habitats. Microarray expression profiling studies of the rugose and smooth variants of the same strain led to the identification of 124 differentially regulated genes. Further expression profiling experiments showed how these genes are regulated by the VpsR and HapR transcription factors, which, respectively, positively and negatively regulate production of VPSEl Tor, a rugose‐associated extracellular polysaccharide. The study of mutants of rpoN and rpoS demonstrated the effects of these alternative sigma factors on phase variation‐specific gene expression. Bioinformatics analysis of these expression data shows that ‘rugosity’ and ‘smoothness’ are determined by a complex hierarchy of positive and negative regulators, which also affect the biofilm, surface hydrophobicity and motility phenotypes of the organism.</description><identifier>ISSN: 0950-382X</identifier><identifier>EISSN: 1365-2958</identifier><identifier>DOI: 10.1111/j.1365-2958.2004.04154.x</identifier><identifier>PMID: 15228530</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Science Ltd</publisher><subject>Adaptation, Physiological ; Bacterial Proteins - genetics ; Bacterial Proteins - physiology ; Bacteriology ; Biofilms - growth & development ; Biological and medical sciences ; DNA-Binding Proteins - genetics ; DNA-Binding Proteins - physiology ; DNA-Directed RNA Polymerases - genetics ; DNA-Directed RNA Polymerases - physiology ; Fundamental and applied biological sciences. Psychology ; Gene Expression Profiling ; Gene Expression Regulation, Bacterial ; Genes, Bacterial ; Hydrophobic and Hydrophilic Interactions ; Microbiology ; Miscellaneous ; Movement ; Mutation ; Oligonucleotide Array Sequence Analysis ; Polysaccharides, Bacterial - biosynthesis ; Polysaccharides, Bacterial - metabolism ; Regulon ; Repressor Proteins - physiology ; RNA Polymerase Sigma 54 ; Sigma Factor - genetics ; Sigma Factor - physiology ; Signal Transduction ; Trans-Activators - physiology ; Vibrio cholerae ; Vibrio cholerae O1 - genetics ; Vibrio cholerae O1 - physiology</subject><ispartof>Molecular microbiology, 2004-07, Vol.53 (2), p.497-515</ispartof><rights>2004 INIST-CNRS</rights><rights>Copyright Blackwell Scientific Publications Ltd. Jul 2004</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5034-df185b38ee4eb1bcb7cc6651bee96e6ba748abfeedaa4956c6c48f79ecd189f03</citedby><cites>FETCH-LOGICAL-c5034-df185b38ee4eb1bcb7cc6651bee96e6ba748abfeedaa4956c6c48f79ecd189f03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1365-2958.2004.04154.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1365-2958.2004.04154.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,1427,27903,27904,45553,45554,46388,46812</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15946002$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15228530$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yildiz, Fitnat H.</creatorcontrib><creatorcontrib>Liu, Xiaole S.</creatorcontrib><creatorcontrib>Heydorn, Arne</creatorcontrib><creatorcontrib>Schoolnik, Gary K.</creatorcontrib><title>Molecular analysis of rugosity in a Vibrio cholerae O1 El Tor phase variant</title><title>Molecular microbiology</title><addtitle>Mol Microbiol</addtitle><description>Summary
Reversible phase variation between the rugose and smooth colony variants is predicted to be important for the survival of Vibrio cholerae in natural aquatic habitats. Microarray expression profiling studies of the rugose and smooth variants of the same strain led to the identification of 124 differentially regulated genes. Further expression profiling experiments showed how these genes are regulated by the VpsR and HapR transcription factors, which, respectively, positively and negatively regulate production of VPSEl Tor, a rugose‐associated extracellular polysaccharide. The study of mutants of rpoN and rpoS demonstrated the effects of these alternative sigma factors on phase variation‐specific gene expression. Bioinformatics analysis of these expression data shows that ‘rugosity’ and ‘smoothness’ are determined by a complex hierarchy of positive and negative regulators, which also affect the biofilm, surface hydrophobicity and motility phenotypes of the organism.</description><subject>Adaptation, Physiological</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - physiology</subject><subject>Bacteriology</subject><subject>Biofilms - growth & development</subject><subject>Biological and medical sciences</subject><subject>DNA-Binding Proteins - genetics</subject><subject>DNA-Binding Proteins - physiology</subject><subject>DNA-Directed RNA Polymerases - genetics</subject><subject>DNA-Directed RNA Polymerases - physiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Regulation, Bacterial</subject><subject>Genes, Bacterial</subject><subject>Hydrophobic and Hydrophilic Interactions</subject><subject>Microbiology</subject><subject>Miscellaneous</subject><subject>Movement</subject><subject>Mutation</subject><subject>Oligonucleotide Array Sequence Analysis</subject><subject>Polysaccharides, Bacterial - biosynthesis</subject><subject>Polysaccharides, Bacterial - metabolism</subject><subject>Regulon</subject><subject>Repressor Proteins - physiology</subject><subject>RNA Polymerase Sigma 54</subject><subject>Sigma Factor - genetics</subject><subject>Sigma Factor - physiology</subject><subject>Signal Transduction</subject><subject>Trans-Activators - physiology</subject><subject>Vibrio cholerae</subject><subject>Vibrio cholerae O1 - genetics</subject><subject>Vibrio cholerae O1 - physiology</subject><issn>0950-382X</issn><issn>1365-2958</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqN0U1vEzEQBmALgWha-AvIQoLbLv5e-8ABVaVUNOqlIG6W7cxSR5t1sLOl-fd4SQSIC_hiS35mNJoXIUxJS-t5s24pV7JhRuqWESJaIqgU7cMjtPj18RgtiJGk4Zp9OUGnpawJoZwo_hSdUMmYlpws0MdlGiBMg8vYjW7Yl1hw6nGevqYSd3scR-zw5-hzTDjcVZsd4BuKLwZ8mzLe3rkC-N7l6MbdM_Skd0OB58f7DH16f3F7_qG5vrm8On933QRJuGhWPdXScw0gwFMffBeCUpJ6AKNAedcJ7XwPsHJOGKmCCkL3nYGwotr0hJ-h14e-25y-TVB2dhNLgGFwI6SpWKVURyn7N6Sdqf2JrvDlX3Cdplz3UY1RkjPFREX6gEJOpWTo7TbHjct7S4mdY7FrO2_fztu3cyz2Zyz2oZa-OPaf_AZWvwuPOVTw6ghcCW7osxtDLH84IxQhrLq3B_c9DrD_7wHscnk1v_gPjw-n1g</recordid><startdate>200407</startdate><enddate>200407</enddate><creator>Yildiz, Fitnat H.</creator><creator>Liu, Xiaole S.</creator><creator>Heydorn, Arne</creator><creator>Schoolnik, Gary K.</creator><general>Blackwell Science Ltd</general><general>Blackwell Science</general><general>Blackwell Publishing Ltd</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>200407</creationdate><title>Molecular analysis of rugosity in a Vibrio cholerae O1 El Tor phase variant</title><author>Yildiz, Fitnat H. ; Liu, Xiaole S. ; Heydorn, Arne ; Schoolnik, Gary K.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5034-df185b38ee4eb1bcb7cc6651bee96e6ba748abfeedaa4956c6c48f79ecd189f03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Adaptation, Physiological</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacterial Proteins - physiology</topic><topic>Bacteriology</topic><topic>Biofilms - growth & development</topic><topic>Biological and medical sciences</topic><topic>DNA-Binding Proteins - genetics</topic><topic>DNA-Binding Proteins - physiology</topic><topic>DNA-Directed RNA Polymerases - genetics</topic><topic>DNA-Directed RNA Polymerases - physiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Profiling</topic><topic>Gene Expression Regulation, Bacterial</topic><topic>Genes, Bacterial</topic><topic>Hydrophobic and Hydrophilic Interactions</topic><topic>Microbiology</topic><topic>Miscellaneous</topic><topic>Movement</topic><topic>Mutation</topic><topic>Oligonucleotide Array Sequence Analysis</topic><topic>Polysaccharides, Bacterial - biosynthesis</topic><topic>Polysaccharides, Bacterial - metabolism</topic><topic>Regulon</topic><topic>Repressor Proteins - physiology</topic><topic>RNA Polymerase Sigma 54</topic><topic>Sigma Factor - genetics</topic><topic>Sigma Factor - physiology</topic><topic>Signal Transduction</topic><topic>Trans-Activators - physiology</topic><topic>Vibrio cholerae</topic><topic>Vibrio cholerae O1 - genetics</topic><topic>Vibrio cholerae O1 - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yildiz, Fitnat H.</creatorcontrib><creatorcontrib>Liu, Xiaole S.</creatorcontrib><creatorcontrib>Heydorn, Arne</creatorcontrib><creatorcontrib>Schoolnik, Gary K.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yildiz, Fitnat H.</au><au>Liu, Xiaole S.</au><au>Heydorn, Arne</au><au>Schoolnik, Gary K.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular analysis of rugosity in a Vibrio cholerae O1 El Tor phase variant</atitle><jtitle>Molecular microbiology</jtitle><addtitle>Mol Microbiol</addtitle><date>2004-07</date><risdate>2004</risdate><volume>53</volume><issue>2</issue><spage>497</spage><epage>515</epage><pages>497-515</pages><issn>0950-382X</issn><eissn>1365-2958</eissn><abstract>Summary
Reversible phase variation between the rugose and smooth colony variants is predicted to be important for the survival of Vibrio cholerae in natural aquatic habitats. Microarray expression profiling studies of the rugose and smooth variants of the same strain led to the identification of 124 differentially regulated genes. Further expression profiling experiments showed how these genes are regulated by the VpsR and HapR transcription factors, which, respectively, positively and negatively regulate production of VPSEl Tor, a rugose‐associated extracellular polysaccharide. The study of mutants of rpoN and rpoS demonstrated the effects of these alternative sigma factors on phase variation‐specific gene expression. Bioinformatics analysis of these expression data shows that ‘rugosity’ and ‘smoothness’ are determined by a complex hierarchy of positive and negative regulators, which also affect the biofilm, surface hydrophobicity and motility phenotypes of the organism.</abstract><cop>Oxford, UK</cop><pub>Blackwell Science Ltd</pub><pmid>15228530</pmid><doi>10.1111/j.1365-2958.2004.04154.x</doi><tpages>19</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Molecular microbiology, 2004-07, Vol.53 (2), p.497-515 |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete; Wiley Online Library Free Content; EZB-FREE-00999 freely available EZB journals; Free Full-Text Journals in Chemistry |
| subjects | Adaptation, Physiological Bacterial Proteins - genetics Bacterial Proteins - physiology Bacteriology Biofilms - growth & development Biological and medical sciences DNA-Binding Proteins - genetics DNA-Binding Proteins - physiology DNA-Directed RNA Polymerases - genetics DNA-Directed RNA Polymerases - physiology Fundamental and applied biological sciences. Psychology Gene Expression Profiling Gene Expression Regulation, Bacterial Genes, Bacterial Hydrophobic and Hydrophilic Interactions Microbiology Miscellaneous Movement Mutation Oligonucleotide Array Sequence Analysis Polysaccharides, Bacterial - biosynthesis Polysaccharides, Bacterial - metabolism Regulon Repressor Proteins - physiology RNA Polymerase Sigma 54 Sigma Factor - genetics Sigma Factor - physiology Signal Transduction Trans-Activators - physiology Vibrio cholerae Vibrio cholerae O1 - genetics Vibrio cholerae O1 - physiology |
| title | Molecular analysis of rugosity in a Vibrio cholerae O1 El Tor phase variant |
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