Quantification of Etoposide and Etoposide Phosphate in Human Plasma by Micellar Electrokinetic Chromatography and Near-Field Thermal Lens Detection
A method employing micellar electrokinetic chromatography in combination with near-field thermal lens detection (NF-TLD) was developed for the rapid simultaneous determination of etoposide phosphate and etoposide in human blood plasma, taking teniposide as internal standard. The method developed all...
Gespeichert in:
| Veröffentlicht in: | Analytical chemistry (Washington) 2004-07, Vol.76 (13), p.3804-3809 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 3809 |
|---|---|
| container_issue | 13 |
| container_start_page | 3804 |
| container_title | Analytical chemistry (Washington) |
| container_volume | 76 |
| creator | Ragozina, Natalia Y Pütz, Michael Heissler, Stefan Faubel, Werner Pyell, Ute |
| description | A method employing micellar electrokinetic chromatography in combination with near-field thermal lens detection (NF-TLD) was developed for the rapid simultaneous determination of etoposide phosphate and etoposide in human blood plasma, taking teniposide as internal standard. The method developed allows the baseline separation of the solutes of interest from each other and from potential interfering matrix constituents within 4 min. The NF-TLD device employed permits detection of solutes absorbing electromagnetic radiation at λ = 257 nm in fused-silica capillaries with 75-μm i.d. via the near-field thermal lens effect with LODs of 100 μg L-1 for etoposide phosphate and 170 μg L-1 for etoposide. Comparison of the performance of this detector to the performance of a commercial absorption spectrometric detector working at λ = 257 nm showed a substantial improvement in detection limits (up to 60-fold improvement) for the near-field thermal lens detector. |
| doi_str_mv | 10.1021/ac0304222 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_66665094</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>66665094</sourcerecordid><originalsourceid>FETCH-LOGICAL-a418t-76cc0c34fc41011d52bdb9e2ffb3af808f7a56237e12742ec85c913f37da96873</originalsourceid><addsrcrecordid>eNqFkd9u0zAUxi0EYl3hghdAFhJIXAT8J4mTy6l0G6KMopVr68SxibckDrYjrc_BC5Ou1TrBBb45svw73zmfP4ReUfKBEkY_giKcpIyxJ2hGM0aSvCjYUzQjhPCECUJO0GkIN4RQSmj-HJ1MECt4VszQ7-8j9NEaqyBa12Nn8DK6wQVbawx9_ei2blwYGoga2x5fjh30eN1C6ABXW_zVKt224PGy1Sp6d2t7Ha3Ci8a7DqL76WFotveKVxp8cm51W-NNo30HLV7pPuBPOk6t0xIv0DMDbdAvD3WOfpwvN4vLZPXt4vPibJVASouYiFwponhqVDrZonXGqroqNTOm4mAKUhgBWc640JSJlGlVZKqk3HBRQ5kXgs_Ru73u4N2vUYcoOxvubfTajUHm08lImf4XpIJTvvvROXrzF3jjRt9PJiSjYgqlzHZj3-8h5V0IXhs5eNuB30pK5C5P-ZDnxL4-CI5Vp-sjeQhwAt4eAAgKWuOhVzY84kpaZGU2ccmesyHqu4d38LcyF1xkcrO-luw6X3-5Sqm8OOqCCkcT_y74B52uwrw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>217882957</pqid></control><display><type>article</type><title>Quantification of Etoposide and Etoposide Phosphate in Human Plasma by Micellar Electrokinetic Chromatography and Near-Field Thermal Lens Detection</title><source>MEDLINE</source><source>ACS Publications</source><creator>Ragozina, Natalia Y ; Pütz, Michael ; Heissler, Stefan ; Faubel, Werner ; Pyell, Ute</creator><creatorcontrib>Ragozina, Natalia Y ; Pütz, Michael ; Heissler, Stefan ; Faubel, Werner ; Pyell, Ute</creatorcontrib><description>A method employing micellar electrokinetic chromatography in combination with near-field thermal lens detection (NF-TLD) was developed for the rapid simultaneous determination of etoposide phosphate and etoposide in human blood plasma, taking teniposide as internal standard. The method developed allows the baseline separation of the solutes of interest from each other and from potential interfering matrix constituents within 4 min. The NF-TLD device employed permits detection of solutes absorbing electromagnetic radiation at λ = 257 nm in fused-silica capillaries with 75-μm i.d. via the near-field thermal lens effect with LODs of 100 μg L-1 for etoposide phosphate and 170 μg L-1 for etoposide. Comparison of the performance of this detector to the performance of a commercial absorption spectrometric detector working at λ = 257 nm showed a substantial improvement in detection limits (up to 60-fold improvement) for the near-field thermal lens detector.</description><identifier>ISSN: 0003-2700</identifier><identifier>EISSN: 1520-6882</identifier><identifier>DOI: 10.1021/ac0304222</identifier><identifier>PMID: 15228358</identifier><identifier>CODEN: ANCHAM</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>Analytical chemistry ; Chemistry ; Chromatographic methods and physical methods associated with chromatography ; Chromatography ; Chromatography, Micellar Electrokinetic Capillary - methods ; Electromagnetism ; Etoposide - analogs & derivatives ; Etoposide - blood ; Etoposide - chemistry ; Exact sciences and technology ; Humans ; Lenses ; Molecular Structure ; Organophosphorus Compounds - blood ; Organophosphorus Compounds - chemistry ; Other chromatographic methods ; Radiation ; Sensitivity and Specificity ; Spectrometric and optical methods ; Surface Properties ; Temperature</subject><ispartof>Analytical chemistry (Washington), 2004-07, Vol.76 (13), p.3804-3809</ispartof><rights>Copyright © 2004 American Chemical Society</rights><rights>2004 INIST-CNRS</rights><rights>Copyright American Chemical Society Jul 1, 2004</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a418t-76cc0c34fc41011d52bdb9e2ffb3af808f7a56237e12742ec85c913f37da96873</citedby><cites>FETCH-LOGICAL-a418t-76cc0c34fc41011d52bdb9e2ffb3af808f7a56237e12742ec85c913f37da96873</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/ac0304222$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/ac0304222$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,780,784,2765,27076,27924,27925,56738,56788</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15918595$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15228358$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ragozina, Natalia Y</creatorcontrib><creatorcontrib>Pütz, Michael</creatorcontrib><creatorcontrib>Heissler, Stefan</creatorcontrib><creatorcontrib>Faubel, Werner</creatorcontrib><creatorcontrib>Pyell, Ute</creatorcontrib><title>Quantification of Etoposide and Etoposide Phosphate in Human Plasma by Micellar Electrokinetic Chromatography and Near-Field Thermal Lens Detection</title><title>Analytical chemistry (Washington)</title><addtitle>Anal. Chem</addtitle><description>A method employing micellar electrokinetic chromatography in combination with near-field thermal lens detection (NF-TLD) was developed for the rapid simultaneous determination of etoposide phosphate and etoposide in human blood plasma, taking teniposide as internal standard. The method developed allows the baseline separation of the solutes of interest from each other and from potential interfering matrix constituents within 4 min. The NF-TLD device employed permits detection of solutes absorbing electromagnetic radiation at λ = 257 nm in fused-silica capillaries with 75-μm i.d. via the near-field thermal lens effect with LODs of 100 μg L-1 for etoposide phosphate and 170 μg L-1 for etoposide. Comparison of the performance of this detector to the performance of a commercial absorption spectrometric detector working at λ = 257 nm showed a substantial improvement in detection limits (up to 60-fold improvement) for the near-field thermal lens detector.</description><subject>Analytical chemistry</subject><subject>Chemistry</subject><subject>Chromatographic methods and physical methods associated with chromatography</subject><subject>Chromatography</subject><subject>Chromatography, Micellar Electrokinetic Capillary - methods</subject><subject>Electromagnetism</subject><subject>Etoposide - analogs & derivatives</subject><subject>Etoposide - blood</subject><subject>Etoposide - chemistry</subject><subject>Exact sciences and technology</subject><subject>Humans</subject><subject>Lenses</subject><subject>Molecular Structure</subject><subject>Organophosphorus Compounds - blood</subject><subject>Organophosphorus Compounds - chemistry</subject><subject>Other chromatographic methods</subject><subject>Radiation</subject><subject>Sensitivity and Specificity</subject><subject>Spectrometric and optical methods</subject><subject>Surface Properties</subject><subject>Temperature</subject><issn>0003-2700</issn><issn>1520-6882</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkd9u0zAUxi0EYl3hghdAFhJIXAT8J4mTy6l0G6KMopVr68SxibckDrYjrc_BC5Ou1TrBBb45svw73zmfP4ReUfKBEkY_giKcpIyxJ2hGM0aSvCjYUzQjhPCECUJO0GkIN4RQSmj-HJ1MECt4VszQ7-8j9NEaqyBa12Nn8DK6wQVbawx9_ei2blwYGoga2x5fjh30eN1C6ABXW_zVKt224PGy1Sp6d2t7Ha3Ci8a7DqL76WFotveKVxp8cm51W-NNo30HLV7pPuBPOk6t0xIv0DMDbdAvD3WOfpwvN4vLZPXt4vPibJVASouYiFwponhqVDrZonXGqroqNTOm4mAKUhgBWc640JSJlGlVZKqk3HBRQ5kXgs_Ru73u4N2vUYcoOxvubfTajUHm08lImf4XpIJTvvvROXrzF3jjRt9PJiSjYgqlzHZj3-8h5V0IXhs5eNuB30pK5C5P-ZDnxL4-CI5Vp-sjeQhwAt4eAAgKWuOhVzY84kpaZGU2ccmesyHqu4d38LcyF1xkcrO-luw6X3-5Sqm8OOqCCkcT_y74B52uwrw</recordid><startdate>20040701</startdate><enddate>20040701</enddate><creator>Ragozina, Natalia Y</creator><creator>Pütz, Michael</creator><creator>Heissler, Stefan</creator><creator>Faubel, Werner</creator><creator>Pyell, Ute</creator><general>American Chemical Society</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7TM</scope><scope>7U5</scope><scope>7U7</scope><scope>7U9</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>H94</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20040701</creationdate><title>Quantification of Etoposide and Etoposide Phosphate in Human Plasma by Micellar Electrokinetic Chromatography and Near-Field Thermal Lens Detection</title><author>Ragozina, Natalia Y ; Pütz, Michael ; Heissler, Stefan ; Faubel, Werner ; Pyell, Ute</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a418t-76cc0c34fc41011d52bdb9e2ffb3af808f7a56237e12742ec85c913f37da96873</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Analytical chemistry</topic><topic>Chemistry</topic><topic>Chromatographic methods and physical methods associated with chromatography</topic><topic>Chromatography</topic><topic>Chromatography, Micellar Electrokinetic Capillary - methods</topic><topic>Electromagnetism</topic><topic>Etoposide - analogs & derivatives</topic><topic>Etoposide - blood</topic><topic>Etoposide - chemistry</topic><topic>Exact sciences and technology</topic><topic>Humans</topic><topic>Lenses</topic><topic>Molecular Structure</topic><topic>Organophosphorus Compounds - blood</topic><topic>Organophosphorus Compounds - chemistry</topic><topic>Other chromatographic methods</topic><topic>Radiation</topic><topic>Sensitivity and Specificity</topic><topic>Spectrometric and optical methods</topic><topic>Surface Properties</topic><topic>Temperature</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ragozina, Natalia Y</creatorcontrib><creatorcontrib>Pütz, Michael</creatorcontrib><creatorcontrib>Heissler, Stefan</creatorcontrib><creatorcontrib>Faubel, Werner</creatorcontrib><creatorcontrib>Pyell, Ute</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical chemistry (Washington)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ragozina, Natalia Y</au><au>Pütz, Michael</au><au>Heissler, Stefan</au><au>Faubel, Werner</au><au>Pyell, Ute</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Quantification of Etoposide and Etoposide Phosphate in Human Plasma by Micellar Electrokinetic Chromatography and Near-Field Thermal Lens Detection</atitle><jtitle>Analytical chemistry (Washington)</jtitle><addtitle>Anal. Chem</addtitle><date>2004-07-01</date><risdate>2004</risdate><volume>76</volume><issue>13</issue><spage>3804</spage><epage>3809</epage><pages>3804-3809</pages><issn>0003-2700</issn><eissn>1520-6882</eissn><coden>ANCHAM</coden><abstract>A method employing micellar electrokinetic chromatography in combination with near-field thermal lens detection (NF-TLD) was developed for the rapid simultaneous determination of etoposide phosphate and etoposide in human blood plasma, taking teniposide as internal standard. The method developed allows the baseline separation of the solutes of interest from each other and from potential interfering matrix constituents within 4 min. The NF-TLD device employed permits detection of solutes absorbing electromagnetic radiation at λ = 257 nm in fused-silica capillaries with 75-μm i.d. via the near-field thermal lens effect with LODs of 100 μg L-1 for etoposide phosphate and 170 μg L-1 for etoposide. Comparison of the performance of this detector to the performance of a commercial absorption spectrometric detector working at λ = 257 nm showed a substantial improvement in detection limits (up to 60-fold improvement) for the near-field thermal lens detector.</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><pmid>15228358</pmid><doi>10.1021/ac0304222</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0003-2700 |
| ispartof | Analytical chemistry (Washington), 2004-07, Vol.76 (13), p.3804-3809 |
| issn | 0003-2700 1520-6882 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_66665094 |
| source | MEDLINE; ACS Publications |
| subjects | Analytical chemistry Chemistry Chromatographic methods and physical methods associated with chromatography Chromatography Chromatography, Micellar Electrokinetic Capillary - methods Electromagnetism Etoposide - analogs & derivatives Etoposide - blood Etoposide - chemistry Exact sciences and technology Humans Lenses Molecular Structure Organophosphorus Compounds - blood Organophosphorus Compounds - chemistry Other chromatographic methods Radiation Sensitivity and Specificity Spectrometric and optical methods Surface Properties Temperature |
| title | Quantification of Etoposide and Etoposide Phosphate in Human Plasma by Micellar Electrokinetic Chromatography and Near-Field Thermal Lens Detection |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-04T09%3A51%3A42IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Quantification%20of%20Etoposide%20and%20Etoposide%20Phosphate%20in%20Human%20Plasma%20by%20Micellar%20Electrokinetic%20Chromatography%20and%20Near-Field%20Thermal%20Lens%20Detection&rft.jtitle=Analytical%20chemistry%20(Washington)&rft.au=Ragozina,%20Natalia%20Y&rft.date=2004-07-01&rft.volume=76&rft.issue=13&rft.spage=3804&rft.epage=3809&rft.pages=3804-3809&rft.issn=0003-2700&rft.eissn=1520-6882&rft.coden=ANCHAM&rft_id=info:doi/10.1021/ac0304222&rft_dat=%3Cproquest_cross%3E66665094%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=217882957&rft_id=info:pmid/15228358&rfr_iscdi=true |