Proteome reference maps of Medicago truncatula embryogenic cell cultures generated from single protoplasts

Using a combination of two‐dimensional gel electrophoresis (2‐DE) protein mapping and mass spectrometry (MS) analysis, we have established proteome reference maps of Medicago truncatula embryogenic tissue culture cells. The cultures were generated from single protoplasts, which provided a relatively...

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Veröffentlicht in:	Proteomics (Weinheim) 2004-07, Vol.4 (7), p.1883-1896
Hauptverfasser:	Imin, Nijat, De Jong, Femke, Mathesius, Ulrike, van Noorden, Giel, Saeed, Nasir A., Wang, Xin-Ding, Rose, Ray J., Rolfe, Barry G.
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Databases as Topic Electrophoresis, Gel, Two-Dimensional Electrophoresis, Polyacrylamide Gel Expressed Sequence Tags Genome Hydrogen-Ion Concentration Image Processing, Computer-Assisted Mass Spectrometry Medicago truncatula Medicago truncatula - genetics Medicago truncatula - metabolism Medicago truncatula Jemalong 2HA Molecular Sequence Data Peptide mass fingerprint Peptides - chemistry Protein Isoforms Protein Structure, Tertiary Proteome Protoplast Protoplasts - metabolism Rosaniline Dyes - pharmacology Sequence Homology, Amino Acid Silver Staining Somatic embryogenesis Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Two-dimensional gel electrophoresis
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