CpG‐containing ODN has a limited role in the protection against Toxoplasma gondii
SUMMARY Bacterial DNA containing immunostimulatory motifs (CpG) induces the development of a TH1 immune response. Since protection against Toxoplasma gondii is correlated with this type of response, the aim of this work was to determine if a synthetic oligodeoxynucleotide (ODN) containing CpG sequen...
Gespeichert in:
| Veröffentlicht in: | Parasite immunology 2004-02, Vol.26 (2), p.67-73 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 73 |
|---|---|
| container_issue | 2 |
| container_start_page | 67 |
| container_title | Parasite immunology |
| container_volume | 26 |
| creator | Saavedra, R. Leyva, R. Tenorio, E. P. Haces, M. L. Rodríguez‐Sosa, M. Terrazas, L. I. Hérion, P. |
| description | SUMMARY
Bacterial DNA containing immunostimulatory motifs (CpG) induces the development of a TH1 immune response. Since protection against Toxoplasma gondii is correlated with this type of response, the aim of this work was to determine if a synthetic oligodeoxynucleotide (ODN) containing CpG sequences could be useful as adjuvant for the induction of a long‐lasting protective immune response against T. gondii. BALB/c mice immunized with a total soluble antigen of T. gondii (TSA2) mixed with ODN‐containing CpG sequences developed a typical TH1 response, as determined by antibody isotypes and interferon‐γ (IFN‐γ) and interleukin‐4 (IL‐4) production by spleen cells. However, they did not resist a challenge with the virulent RH strain of the parasite. Absence of protection paralleled with lower levels of IFN‐γ, when compared with mice vaccinated with the live tachyzoites of the attenuated ts.4 strain of the parasite, which resisted this challenge. Intraperitoneal injection of ODN alone to mice induced a high degree of resistance to a lethal challenge inoculated by the same route. Nevertheless, this nonspecific protection was transient. Thus, the use of ODN containing CpG motifs as adjuvant is of limited value for the induction of a protective immune response against T. gondii. |
| doi_str_mv | 10.1111/j.0141-9838.2004.00684.x |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_66664574</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>66664574</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4904-2a77a3b28f6259b7cb7bac26f14d46b28b4cd16a30f68cd6aa060e8b9fbe05fc3</originalsourceid><addsrcrecordid>eNqNkE1OwzAQRi0EoqVwBeQVuwT_xUkkNqhAqVQoEmVtOY7TukriEqei3XEEzshJcGgFS5jNjKz3zVgPAIhRiH1dLkOEGQ7ShCYhQYiFCPGEhZsD0MeURwFFhB2C_g_UAyfOLRHClHB6DHo4IiQiKe2D5-Fq9Pn-oWzdSlObeg6nN49wIR2UsDSVaXUOG1tqaGrYLjRcNbbVqjW2hnLuE66FM7uxq1K6SsK5rXNjTsFRIUunz_Z9AF7ubmfD-2AyHY2H15NAsRSxgMg4ljQjScFJlGaxyuJMKsILzHLG_XvGVI65pKjgicq5lIgjnWRpkWkUFYoOwMVur__U61q7VlTGKV2WstZ27QT3xaKY_QniBEVeK_VgsgNVY51rdCFWjalksxUYic68WIpOquikis68-DYvNj56vr-xziqd_wb3qj1wtQPeTKm3_14snsYPfqBfPTCSsQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>18051113</pqid></control><display><type>article</type><title>CpG‐containing ODN has a limited role in the protection against Toxoplasma gondii</title><source>MEDLINE</source><source>Wiley Journals</source><source>IngentaConnect Free/Open Access Journals</source><source>EZB-FREE-00999 freely available EZB journals</source><source>Wiley Online Library (Open Access Collection)</source><source>Alma/SFX Local Collection</source><creator>Saavedra, R. ; Leyva, R. ; Tenorio, E. P. ; Haces, M. L. ; Rodríguez‐Sosa, M. ; Terrazas, L. I. ; Hérion, P.</creator><creatorcontrib>Saavedra, R. ; Leyva, R. ; Tenorio, E. P. ; Haces, M. L. ; Rodríguez‐Sosa, M. ; Terrazas, L. I. ; Hérion, P.</creatorcontrib><description>SUMMARY
Bacterial DNA containing immunostimulatory motifs (CpG) induces the development of a TH1 immune response. Since protection against Toxoplasma gondii is correlated with this type of response, the aim of this work was to determine if a synthetic oligodeoxynucleotide (ODN) containing CpG sequences could be useful as adjuvant for the induction of a long‐lasting protective immune response against T. gondii. BALB/c mice immunized with a total soluble antigen of T. gondii (TSA2) mixed with ODN‐containing CpG sequences developed a typical TH1 response, as determined by antibody isotypes and interferon‐γ (IFN‐γ) and interleukin‐4 (IL‐4) production by spleen cells. However, they did not resist a challenge with the virulent RH strain of the parasite. Absence of protection paralleled with lower levels of IFN‐γ, when compared with mice vaccinated with the live tachyzoites of the attenuated ts.4 strain of the parasite, which resisted this challenge. Intraperitoneal injection of ODN alone to mice induced a high degree of resistance to a lethal challenge inoculated by the same route. Nevertheless, this nonspecific protection was transient. Thus, the use of ODN containing CpG motifs as adjuvant is of limited value for the induction of a protective immune response against T. gondii.</description><identifier>ISSN: 0141-9838</identifier><identifier>EISSN: 1365-3024</identifier><identifier>DOI: 10.1111/j.0141-9838.2004.00684.x</identifier><identifier>PMID: 15225293</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Science Ltd</publisher><subject>adjuvant ; Adjuvants, Immunologic - administration & dosage ; Animals ; Antigens, Protozoan - administration & dosage ; CpG motifs ; Female ; Immunization ; Interferon-gamma - biosynthesis ; Interleukin-4 - biosynthesis ; Mice ; Mice, Inbred BALB C ; ODN ; Oligodeoxyribonucleotides - administration & dosage ; Protozoan Vaccines - administration & dosage ; Th1 Cells - immunology ; Toxoplasma - pathogenicity ; Toxoplasma gondii ; Toxoplasmosis - immunology ; Toxoplasmosis - prevention & control</subject><ispartof>Parasite immunology, 2004-02, Vol.26 (2), p.67-73</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4904-2a77a3b28f6259b7cb7bac26f14d46b28b4cd16a30f68cd6aa060e8b9fbe05fc3</citedby><cites>FETCH-LOGICAL-c4904-2a77a3b28f6259b7cb7bac26f14d46b28b4cd16a30f68cd6aa060e8b9fbe05fc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.0141-9838.2004.00684.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.0141-9838.2004.00684.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,1433,27924,27925,45574,45575,46409,46833</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15225293$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Saavedra, R.</creatorcontrib><creatorcontrib>Leyva, R.</creatorcontrib><creatorcontrib>Tenorio, E. P.</creatorcontrib><creatorcontrib>Haces, M. L.</creatorcontrib><creatorcontrib>Rodríguez‐Sosa, M.</creatorcontrib><creatorcontrib>Terrazas, L. I.</creatorcontrib><creatorcontrib>Hérion, P.</creatorcontrib><title>CpG‐containing ODN has a limited role in the protection against Toxoplasma gondii</title><title>Parasite immunology</title><addtitle>Parasite Immunol</addtitle><description>SUMMARY
Bacterial DNA containing immunostimulatory motifs (CpG) induces the development of a TH1 immune response. Since protection against Toxoplasma gondii is correlated with this type of response, the aim of this work was to determine if a synthetic oligodeoxynucleotide (ODN) containing CpG sequences could be useful as adjuvant for the induction of a long‐lasting protective immune response against T. gondii. BALB/c mice immunized with a total soluble antigen of T. gondii (TSA2) mixed with ODN‐containing CpG sequences developed a typical TH1 response, as determined by antibody isotypes and interferon‐γ (IFN‐γ) and interleukin‐4 (IL‐4) production by spleen cells. However, they did not resist a challenge with the virulent RH strain of the parasite. Absence of protection paralleled with lower levels of IFN‐γ, when compared with mice vaccinated with the live tachyzoites of the attenuated ts.4 strain of the parasite, which resisted this challenge. Intraperitoneal injection of ODN alone to mice induced a high degree of resistance to a lethal challenge inoculated by the same route. Nevertheless, this nonspecific protection was transient. Thus, the use of ODN containing CpG motifs as adjuvant is of limited value for the induction of a protective immune response against T. gondii.</description><subject>adjuvant</subject><subject>Adjuvants, Immunologic - administration & dosage</subject><subject>Animals</subject><subject>Antigens, Protozoan - administration & dosage</subject><subject>CpG motifs</subject><subject>Female</subject><subject>Immunization</subject><subject>Interferon-gamma - biosynthesis</subject><subject>Interleukin-4 - biosynthesis</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>ODN</subject><subject>Oligodeoxyribonucleotides - administration & dosage</subject><subject>Protozoan Vaccines - administration & dosage</subject><subject>Th1 Cells - immunology</subject><subject>Toxoplasma - pathogenicity</subject><subject>Toxoplasma gondii</subject><subject>Toxoplasmosis - immunology</subject><subject>Toxoplasmosis - prevention & control</subject><issn>0141-9838</issn><issn>1365-3024</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkE1OwzAQRi0EoqVwBeQVuwT_xUkkNqhAqVQoEmVtOY7TukriEqei3XEEzshJcGgFS5jNjKz3zVgPAIhRiH1dLkOEGQ7ShCYhQYiFCPGEhZsD0MeURwFFhB2C_g_UAyfOLRHClHB6DHo4IiQiKe2D5-Fq9Pn-oWzdSlObeg6nN49wIR2UsDSVaXUOG1tqaGrYLjRcNbbVqjW2hnLuE66FM7uxq1K6SsK5rXNjTsFRIUunz_Z9AF7ubmfD-2AyHY2H15NAsRSxgMg4ljQjScFJlGaxyuJMKsILzHLG_XvGVI65pKjgicq5lIgjnWRpkWkUFYoOwMVur__U61q7VlTGKV2WstZ27QT3xaKY_QniBEVeK_VgsgNVY51rdCFWjalksxUYic68WIpOquikis68-DYvNj56vr-xziqd_wb3qj1wtQPeTKm3_14snsYPfqBfPTCSsQ</recordid><startdate>200402</startdate><enddate>200402</enddate><creator>Saavedra, R.</creator><creator>Leyva, R.</creator><creator>Tenorio, E. P.</creator><creator>Haces, M. L.</creator><creator>Rodríguez‐Sosa, M.</creator><creator>Terrazas, L. I.</creator><creator>Hérion, P.</creator><general>Blackwell Science Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>200402</creationdate><title>CpG‐containing ODN has a limited role in the protection against Toxoplasma gondii</title><author>Saavedra, R. ; Leyva, R. ; Tenorio, E. P. ; Haces, M. L. ; Rodríguez‐Sosa, M. ; Terrazas, L. I. ; Hérion, P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4904-2a77a3b28f6259b7cb7bac26f14d46b28b4cd16a30f68cd6aa060e8b9fbe05fc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>adjuvant</topic><topic>Adjuvants, Immunologic - administration & dosage</topic><topic>Animals</topic><topic>Antigens, Protozoan - administration & dosage</topic><topic>CpG motifs</topic><topic>Female</topic><topic>Immunization</topic><topic>Interferon-gamma - biosynthesis</topic><topic>Interleukin-4 - biosynthesis</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>ODN</topic><topic>Oligodeoxyribonucleotides - administration & dosage</topic><topic>Protozoan Vaccines - administration & dosage</topic><topic>Th1 Cells - immunology</topic><topic>Toxoplasma - pathogenicity</topic><topic>Toxoplasma gondii</topic><topic>Toxoplasmosis - immunology</topic><topic>Toxoplasmosis - prevention & control</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Saavedra, R.</creatorcontrib><creatorcontrib>Leyva, R.</creatorcontrib><creatorcontrib>Tenorio, E. P.</creatorcontrib><creatorcontrib>Haces, M. L.</creatorcontrib><creatorcontrib>Rodríguez‐Sosa, M.</creatorcontrib><creatorcontrib>Terrazas, L. I.</creatorcontrib><creatorcontrib>Hérion, P.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>Parasite immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Saavedra, R.</au><au>Leyva, R.</au><au>Tenorio, E. P.</au><au>Haces, M. L.</au><au>Rodríguez‐Sosa, M.</au><au>Terrazas, L. I.</au><au>Hérion, P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>CpG‐containing ODN has a limited role in the protection against Toxoplasma gondii</atitle><jtitle>Parasite immunology</jtitle><addtitle>Parasite Immunol</addtitle><date>2004-02</date><risdate>2004</risdate><volume>26</volume><issue>2</issue><spage>67</spage><epage>73</epage><pages>67-73</pages><issn>0141-9838</issn><eissn>1365-3024</eissn><abstract>SUMMARY
Bacterial DNA containing immunostimulatory motifs (CpG) induces the development of a TH1 immune response. Since protection against Toxoplasma gondii is correlated with this type of response, the aim of this work was to determine if a synthetic oligodeoxynucleotide (ODN) containing CpG sequences could be useful as adjuvant for the induction of a long‐lasting protective immune response against T. gondii. BALB/c mice immunized with a total soluble antigen of T. gondii (TSA2) mixed with ODN‐containing CpG sequences developed a typical TH1 response, as determined by antibody isotypes and interferon‐γ (IFN‐γ) and interleukin‐4 (IL‐4) production by spleen cells. However, they did not resist a challenge with the virulent RH strain of the parasite. Absence of protection paralleled with lower levels of IFN‐γ, when compared with mice vaccinated with the live tachyzoites of the attenuated ts.4 strain of the parasite, which resisted this challenge. Intraperitoneal injection of ODN alone to mice induced a high degree of resistance to a lethal challenge inoculated by the same route. Nevertheless, this nonspecific protection was transient. Thus, the use of ODN containing CpG motifs as adjuvant is of limited value for the induction of a protective immune response against T. gondii.</abstract><cop>Oxford, UK</cop><pub>Blackwell Science Ltd</pub><pmid>15225293</pmid><doi>10.1111/j.0141-9838.2004.00684.x</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0141-9838 |
| ispartof | Parasite immunology, 2004-02, Vol.26 (2), p.67-73 |
| issn | 0141-9838 1365-3024 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_66664574 |
| source | MEDLINE; Wiley Journals; IngentaConnect Free/Open Access Journals; EZB-FREE-00999 freely available EZB journals; Wiley Online Library (Open Access Collection); Alma/SFX Local Collection |
| subjects | adjuvant Adjuvants, Immunologic - administration & dosage Animals Antigens, Protozoan - administration & dosage CpG motifs Female Immunization Interferon-gamma - biosynthesis Interleukin-4 - biosynthesis Mice Mice, Inbred BALB C ODN Oligodeoxyribonucleotides - administration & dosage Protozoan Vaccines - administration & dosage Th1 Cells - immunology Toxoplasma - pathogenicity Toxoplasma gondii Toxoplasmosis - immunology Toxoplasmosis - prevention & control |
| title | CpG‐containing ODN has a limited role in the protection against Toxoplasma gondii |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-02T11%3A35%3A53IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=CpG%E2%80%90containing%20ODN%20has%20a%20limited%20role%20in%20the%20protection%20against%20Toxoplasma%20gondii&rft.jtitle=Parasite%20immunology&rft.au=Saavedra,%20R.&rft.date=2004-02&rft.volume=26&rft.issue=2&rft.spage=67&rft.epage=73&rft.pages=67-73&rft.issn=0141-9838&rft.eissn=1365-3024&rft_id=info:doi/10.1111/j.0141-9838.2004.00684.x&rft_dat=%3Cproquest_cross%3E66664574%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=18051113&rft_id=info:pmid/15225293&rfr_iscdi=true |