Classical and molecular cytogenetic characterization of allochthonous European bitterling Rhodeus amarus (Cyprinidae, Acheilognathinae) from northern Italy
A cytogenetical study was carried out on 34 specimens of the European bitterling Rhodeus amarus (Teleostei: Cyprinidae, Acheilognathinae) from four rivers of the Venice district (NE Italy). This allochthonous fish species was accidentally introduced in the North-East of Italy about 20 years ago and...
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| Veröffentlicht in: | Genes & Genetic Systems 2008, Vol.83(5), pp.417-422 |
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| creator | Libertini, A.(Institute of Marine Sciences, Venice (Italy)) Sola, L Rampin, M Rossi, A.R Iijima, K Ueda, T |
| description | A cytogenetical study was carried out on 34 specimens of the European bitterling Rhodeus amarus (Teleostei: Cyprinidae, Acheilognathinae) from four rivers of the Venice district (NE Italy). This allochthonous fish species was accidentally introduced in the North-East of Italy about 20 years ago and is now rapidly spreading all over the rivers of the Northern part of the country. All the studied specimens are characterised by the same karyotype (2n = 48: 8M + 20SM + 20ST), i.e., the typical one of the native populations of the species. However, a polymorphism in the number of NOR bearing chromosomes has been found. In fact, in addition to the main species-specific NORs, on the short arms of chromosome pair 7, two to five additional 18S rDNA sites have been revealed by FISH in different specimens. Sequential staining with silver nitrate, chromomycin A3 and DAPI revealed that most of the additional sites are inactive and CMA3-positive. Data herein reported confirm that in spite of an overall morphological karyological conservativeness, significant differences for the finer cytogenetic features can be found within the Acheilognathinae with the 2n = 48 and NF = 76 karyotype. |
| doi_str_mv | 10.1266/ggs.83.417 |
| format | Article |
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This allochthonous fish species was accidentally introduced in the North-East of Italy about 20 years ago and is now rapidly spreading all over the rivers of the Northern part of the country. All the studied specimens are characterised by the same karyotype (2n = 48: 8M + 20SM + 20ST), i.e., the typical one of the native populations of the species. However, a polymorphism in the number of NOR bearing chromosomes has been found. In fact, in addition to the main species-specific NORs, on the short arms of chromosome pair 7, two to five additional 18S rDNA sites have been revealed by FISH in different specimens. Sequential staining with silver nitrate, chromomycin A3 and DAPI revealed that most of the additional sites are inactive and CMA3-positive. Data herein reported confirm that in spite of an overall morphological karyological conservativeness, significant differences for the finer cytogenetic features can be found within the Acheilognathinae with the 2n = 48 and NF = 76 karyotype.</description><identifier>ISSN: 1341-7568</identifier><identifier>EISSN: 1880-5779</identifier><identifier>DOI: 10.1266/ggs.83.417</identifier><identifier>PMID: 19168992</identifier><language>eng</language><publisher>Japan: The Genetics Society of Japan</publisher><subject>Acheilognathinae ; Animals ; CARIOTIPOS ; CARYOTYPE ; Chromosomes - genetics ; CITOGENETICA ; CONSERVACION DE LA NATURALEZA ; CONSERVATION DE LA NATURE ; Cyprinidae ; Cyprinidae - genetics ; CYTOGENETICS ; CYTOGENETIQUE ; fluorescence in situ hybridization ; freshwater fish conservation ; In Situ Hybridization, Fluorescence ; Italy ; karyotype polymorphism ; KARYOTYPES ; Karyotyping ; NATURE CONSERVATION ; POLIMORFISMO ; POLYMORPHISM ; POLYMORPHISME ; rDNA ; RHODEUS AMARUS ; Teleostei</subject><ispartof>Genes & Genetic Systems, 2008, Vol.83(5), pp.417-422</ispartof><rights>2008 by The Genetics Society of Japan</rights><rights>Copyright Japan Science and Technology Agency 2008</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c700t-720ef6fab0c1003821306ce849408137add915c4a6d0e76c5f5cb4d1b1069fb23</citedby><cites>FETCH-LOGICAL-c700t-720ef6fab0c1003821306ce849408137add915c4a6d0e76c5f5cb4d1b1069fb23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,1882,4023,27922,27923,27924</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19168992$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Libertini, A.(Institute of Marine Sciences, Venice (Italy))</creatorcontrib><creatorcontrib>Sola, L</creatorcontrib><creatorcontrib>Rampin, M</creatorcontrib><creatorcontrib>Rossi, A.R</creatorcontrib><creatorcontrib>Iijima, K</creatorcontrib><creatorcontrib>Ueda, T</creatorcontrib><creatorcontrib>“La Sapienza”Rome University</creatorcontrib><creatorcontrib>National Council for Research</creatorcontrib><creatorcontrib>Department of Animal and Human Biology</creatorcontrib><creatorcontrib>Faculty of Education</creatorcontrib><creatorcontrib>Institute of Marine Sciences</creatorcontrib><creatorcontrib>Department of Biology</creatorcontrib><creatorcontrib>Utsunomiya University</creatorcontrib><title>Classical and molecular cytogenetic characterization of allochthonous European bitterling Rhodeus amarus (Cyprinidae, Acheilognathinae) from northern Italy</title><title>Genes & Genetic Systems</title><addtitle>Genes Genet. Syst.</addtitle><description>A cytogenetical study was carried out on 34 specimens of the European bitterling Rhodeus amarus (Teleostei: Cyprinidae, Acheilognathinae) from four rivers of the Venice district (NE Italy). This allochthonous fish species was accidentally introduced in the North-East of Italy about 20 years ago and is now rapidly spreading all over the rivers of the Northern part of the country. All the studied specimens are characterised by the same karyotype (2n = 48: 8M + 20SM + 20ST), i.e., the typical one of the native populations of the species. However, a polymorphism in the number of NOR bearing chromosomes has been found. In fact, in addition to the main species-specific NORs, on the short arms of chromosome pair 7, two to five additional 18S rDNA sites have been revealed by FISH in different specimens. Sequential staining with silver nitrate, chromomycin A3 and DAPI revealed that most of the additional sites are inactive and CMA3-positive. Data herein reported confirm that in spite of an overall morphological karyological conservativeness, significant differences for the finer cytogenetic features can be found within the Acheilognathinae with the 2n = 48 and NF = 76 karyotype.</description><subject>Acheilognathinae</subject><subject>Animals</subject><subject>CARIOTIPOS</subject><subject>CARYOTYPE</subject><subject>Chromosomes - genetics</subject><subject>CITOGENETICA</subject><subject>CONSERVACION DE LA NATURALEZA</subject><subject>CONSERVATION DE LA NATURE</subject><subject>Cyprinidae</subject><subject>Cyprinidae - genetics</subject><subject>CYTOGENETICS</subject><subject>CYTOGENETIQUE</subject><subject>fluorescence in situ hybridization</subject><subject>freshwater fish conservation</subject><subject>In Situ Hybridization, Fluorescence</subject><subject>Italy</subject><subject>karyotype polymorphism</subject><subject>KARYOTYPES</subject><subject>Karyotyping</subject><subject>NATURE CONSERVATION</subject><subject>POLIMORFISMO</subject><subject>POLYMORPHISM</subject><subject>POLYMORPHISME</subject><subject>rDNA</subject><subject>RHODEUS AMARUS</subject><subject>Teleostei</subject><issn>1341-7568</issn><issn>1880-5779</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkl-L1DAUxYso7rr64rsSEETFGfOvafoky7CrKwuK6HPIpGmbJZOMSfowfhW_rLd01gVftNDcwv31nCT3VNVTgteECvFuGPJasjUnzb3qlEiJV3XTtPfhm3GyamohT6pHOd9gTHEr2cPqhLREyLalp9Wvjdc5O6M90qFDu-itmbxOyBxKHGywxRlkRp20KTa5n7q4GFDskfY-mrGMMcQpo4spxb3VAW1dAc67MKCvY-ws9PROJyivNod9csF12r5F52a0zsch6DK6oO1r1Ke4QyGmMtoU0FXR_vC4etBrn-2TYz2rvl9efNt8XF1__nC1Ob9emQbjsmootr3o9RYbgjGTlDAsjJW85VgS1uiua0ltuBYdto0wdV-bLe_IlmDR9lvKzqqXi-4-xR-TzUXtXDbWex0sHE4JeBim_wYp5pJRTP4HrGlNZ_DFX-BNnFKA0yrCBWe8Jo0E6s1CmRRzTrZXcJNwqwdFsJojoCACSjIFEQD4-VFy2u5sd4ceZw7A5QJAdx58DDAue2dsRgZyh3mXWCp4Ga6hCIVBHhZKm0ZyOju9X4RuctGD_eOkE4TG29tN1csy_3zbmQOlbACFZ4tCr6PSQ3JZffoCti0GW8zZb6fW5Jc</recordid><startdate>2008</startdate><enddate>2008</enddate><creator>Libertini, A.(Institute of Marine Sciences, Venice (Italy))</creator><creator>Sola, L</creator><creator>Rampin, M</creator><creator>Rossi, A.R</creator><creator>Iijima, K</creator><creator>Ueda, T</creator><general>The Genetics Society of Japan</general><general>Japan Science and Technology Agency</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SS</scope><scope>7TK</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>NAPCQ</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>2008</creationdate><title>Classical and molecular cytogenetic characterization of allochthonous European bitterling Rhodeus amarus (Cyprinidae, Acheilognathinae) from northern Italy</title><author>Libertini, A.(Institute of Marine Sciences, Venice (Italy)) ; Sola, L ; Rampin, M ; Rossi, A.R ; Iijima, K ; Ueda, T</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c700t-720ef6fab0c1003821306ce849408137add915c4a6d0e76c5f5cb4d1b1069fb23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Acheilognathinae</topic><topic>Animals</topic><topic>CARIOTIPOS</topic><topic>CARYOTYPE</topic><topic>Chromosomes - genetics</topic><topic>CITOGENETICA</topic><topic>CONSERVACION DE LA NATURALEZA</topic><topic>CONSERVATION DE LA NATURE</topic><topic>Cyprinidae</topic><topic>Cyprinidae - genetics</topic><topic>CYTOGENETICS</topic><topic>CYTOGENETIQUE</topic><topic>fluorescence in situ hybridization</topic><topic>freshwater fish conservation</topic><topic>In Situ Hybridization, Fluorescence</topic><topic>Italy</topic><topic>karyotype polymorphism</topic><topic>KARYOTYPES</topic><topic>Karyotyping</topic><topic>NATURE CONSERVATION</topic><topic>POLIMORFISMO</topic><topic>POLYMORPHISM</topic><topic>POLYMORPHISME</topic><topic>rDNA</topic><topic>RHODEUS AMARUS</topic><topic>Teleostei</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Libertini, A.(Institute of Marine Sciences, Venice (Italy))</creatorcontrib><creatorcontrib>Sola, L</creatorcontrib><creatorcontrib>Rampin, M</creatorcontrib><creatorcontrib>Rossi, A.R</creatorcontrib><creatorcontrib>Iijima, K</creatorcontrib><creatorcontrib>Ueda, T</creatorcontrib><creatorcontrib>“La Sapienza”Rome University</creatorcontrib><creatorcontrib>National Council for Research</creatorcontrib><creatorcontrib>Department of Animal and Human Biology</creatorcontrib><creatorcontrib>Faculty of Education</creatorcontrib><creatorcontrib>Institute of Marine Sciences</creatorcontrib><creatorcontrib>Department of Biology</creatorcontrib><creatorcontrib>Utsunomiya University</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Neurosciences Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Premium</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Genes & Genetic Systems</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Libertini, A.(Institute of Marine Sciences, Venice (Italy))</au><au>Sola, L</au><au>Rampin, M</au><au>Rossi, A.R</au><au>Iijima, K</au><au>Ueda, T</au><aucorp>“La Sapienza”Rome University</aucorp><aucorp>National Council for Research</aucorp><aucorp>Department of Animal and Human Biology</aucorp><aucorp>Faculty of Education</aucorp><aucorp>Institute of Marine Sciences</aucorp><aucorp>Department of Biology</aucorp><aucorp>Utsunomiya University</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Classical and molecular cytogenetic characterization of allochthonous European bitterling Rhodeus amarus (Cyprinidae, Acheilognathinae) from northern Italy</atitle><jtitle>Genes & Genetic Systems</jtitle><addtitle>Genes Genet. Syst.</addtitle><date>2008</date><risdate>2008</risdate><volume>83</volume><issue>5</issue><spage>417</spage><epage>422</epage><pages>417-422</pages><issn>1341-7568</issn><eissn>1880-5779</eissn><abstract>A cytogenetical study was carried out on 34 specimens of the European bitterling Rhodeus amarus (Teleostei: Cyprinidae, Acheilognathinae) from four rivers of the Venice district (NE Italy). This allochthonous fish species was accidentally introduced in the North-East of Italy about 20 years ago and is now rapidly spreading all over the rivers of the Northern part of the country. All the studied specimens are characterised by the same karyotype (2n = 48: 8M + 20SM + 20ST), i.e., the typical one of the native populations of the species. However, a polymorphism in the number of NOR bearing chromosomes has been found. In fact, in addition to the main species-specific NORs, on the short arms of chromosome pair 7, two to five additional 18S rDNA sites have been revealed by FISH in different specimens. Sequential staining with silver nitrate, chromomycin A3 and DAPI revealed that most of the additional sites are inactive and CMA3-positive. Data herein reported confirm that in spite of an overall morphological karyological conservativeness, significant differences for the finer cytogenetic features can be found within the Acheilognathinae with the 2n = 48 and NF = 76 karyotype.</abstract><cop>Japan</cop><pub>The Genetics Society of Japan</pub><pmid>19168992</pmid><doi>10.1266/ggs.83.417</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Genes & Genetic Systems, 2008, Vol.83(5), pp.417-422 |
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| subjects | Acheilognathinae Animals CARIOTIPOS CARYOTYPE Chromosomes - genetics CITOGENETICA CONSERVACION DE LA NATURALEZA CONSERVATION DE LA NATURE Cyprinidae Cyprinidae - genetics CYTOGENETICS CYTOGENETIQUE fluorescence in situ hybridization freshwater fish conservation In Situ Hybridization, Fluorescence Italy karyotype polymorphism KARYOTYPES Karyotyping NATURE CONSERVATION POLIMORFISMO POLYMORPHISM POLYMORPHISME rDNA RHODEUS AMARUS Teleostei |
| title | Classical and molecular cytogenetic characterization of allochthonous European bitterling Rhodeus amarus (Cyprinidae, Acheilognathinae) from northern Italy |
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