Molecular detection of GES-2 extended spectrum β-lactamase producing Pseudomonas aeruginosa in Pretoria, South Africa
Screening for and detection of the novel extended spectrum β-lactamase (ESBL), GES-2 produced by Pseudomonas aeruginosa remains a problem in the clinical microbiology laboratory. This study aimed to compare the normally used ESBL screening agent ceftazidime, with molecular detection, to demonstrate...
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| Veröffentlicht in: | International journal of antimicrobial agents 2004-07, Vol.24 (1), p.35-38 |
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| container_title | International journal of antimicrobial agents |
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| creator | Weldhagen, Gerhard F Prinsloo, Andrea |
| description | Screening for and detection of the novel extended spectrum β-lactamase (ESBL), GES-2 produced by
Pseudomonas aeruginosa remains a problem in the clinical microbiology laboratory. This study aimed to compare the normally used ESBL screening agent ceftazidime, with molecular detection, to demonstrate the presence of GES-2 ESBL production in clinical isolates of
P. aeruginosa. Ceftazidime was found unreliable as an ESBL screening agent, with a specificity of 34.4%, when National Committee for Clinical Laboratory Standards resistance criteria for
P. aeruginosa were employed. An improved PCR detection method was devised, that amplified a 371
bp segment of
bla
GES-2. This should lead to more cost-effective DNA sequencing and sequence interpretation in the laboratory. |
| doi_str_mv | 10.1016/j.ijantimicag.2003.12.012 |
| format | Article |
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Pseudomonas aeruginosa remains a problem in the clinical microbiology laboratory. This study aimed to compare the normally used ESBL screening agent ceftazidime, with molecular detection, to demonstrate the presence of GES-2 ESBL production in clinical isolates of
P. aeruginosa. Ceftazidime was found unreliable as an ESBL screening agent, with a specificity of 34.4%, when National Committee for Clinical Laboratory Standards resistance criteria for
P. aeruginosa were employed. An improved PCR detection method was devised, that amplified a 371
bp segment of
bla
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Pseudomonas aeruginosa remains a problem in the clinical microbiology laboratory. This study aimed to compare the normally used ESBL screening agent ceftazidime, with molecular detection, to demonstrate the presence of GES-2 ESBL production in clinical isolates of
P. aeruginosa. Ceftazidime was found unreliable as an ESBL screening agent, with a specificity of 34.4%, when National Committee for Clinical Laboratory Standards resistance criteria for
P. aeruginosa were employed. An improved PCR detection method was devised, that amplified a 371
bp segment of
bla
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Pseudomonas aeruginosa remains a problem in the clinical microbiology laboratory. This study aimed to compare the normally used ESBL screening agent ceftazidime, with molecular detection, to demonstrate the presence of GES-2 ESBL production in clinical isolates of
P. aeruginosa. Ceftazidime was found unreliable as an ESBL screening agent, with a specificity of 34.4%, when National Committee for Clinical Laboratory Standards resistance criteria for
P. aeruginosa were employed. An improved PCR detection method was devised, that amplified a 371
bp segment of
bla
GES-2. This should lead to more cost-effective DNA sequencing and sequence interpretation in the laboratory.</abstract><cop>London</cop><cop>Amsterdam</cop><cop>New York, NY</cop><pub>Elsevier B.V</pub><pmid>15225858</pmid><doi>10.1016/j.ijantimicag.2003.12.012</doi><tpages>4</tpages></addata></record> |
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| ispartof | International journal of antimicrobial agents, 2004-07, Vol.24 (1), p.35-38 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Bacteriology Base Sequence beta-Lactamases - biosynthesis Biological and medical sciences DNA, Bacterial ESBL Fundamental and applied biological sciences. Psychology Metabolism. Enzymes Microbiology Molecular Sequence Data PCR detection Pseudomonas aeruginosa Pseudomonas aeruginosa - enzymology South Africa |
| title | Molecular detection of GES-2 extended spectrum β-lactamase producing Pseudomonas aeruginosa in Pretoria, South Africa |
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