Alterations in tumor necrosis factor–induced endothelial cell procoagulant activity by hyperthermia
TNF is a cytokine with potent antitumor activity in murine models and when administered clinically via regional perfusion. There is substantial evidence that this antitumor activity depends in large part on TNF's procoagulant effect on tumor neovasculature, which is mediated by induction of end...
Gespeichert in:
Veröffentlicht in: | International journal of cancer 2004-09, Vol.111 (3), p.457-462 |
---|---|
Hauptverfasser: | , , , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
container_end_page | 462 |
---|---|
container_issue | 3 |
container_start_page | 457 |
container_title | International journal of cancer |
container_volume | 111 |
creator | Carroll, Nancy M. Elaraj, Dina M. Puhlmann, Markus Weinreich, David M. Turner, Ewa M. Xu, Hui Alexander, H. Richard |
description | TNF is a cytokine with potent antitumor activity in murine models and when administered clinically via regional perfusion. There is substantial evidence that this antitumor activity depends in large part on TNF's procoagulant effect on tumor neovasculature, which is mediated by induction of endothelial cell tissue factor (TF), a component of the extrinsic clotting cascade. In regional perfusion of a cancer‐bearing limb or organ, TNF is always administered under hyperthermic temperatures; however, little is known about the effect of hyperthermia on TNF‐mediated procoagulant activity in endothelium. We examined the effects of hyperthermia on TNF‐mediated procoagulant activity in human umbilical vein endothelial cells (HUVECs). HUVECs were exposed to TNF at normothermic (37°C) and hyperthermic (41°C) temperatures for 90 min, then assayed for clotting activity, TF protein production and mRNA production of TF and tissue factor pathway inhibitor‐2 (TFPI‐2), an endogenous inducible inhibitor of TF activity in HUVECs. TNF treatment at 41°C significantly reduced clotting activity, TF protein and mRNA as well as TFPI‐2 mRNA compared to treatment at 37°C. These data show that hyperthermia significantly reduces the procoagulant effects of TNF on endothelial tissue compared to normothermia, which may have important clinical implications for the use of TNF in regional perfusion. © 2004 Wiley‐Liss, Inc. |
doi_str_mv | 10.1002/ijc.20272 |
format | Article |
fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_66657922</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>66657922</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3512-671ee92dfe60e0921194a4f4ebe53f437efca656e9d058c90aa5f0a390c676dd3</originalsourceid><addsrcrecordid>eNp10M9u1DAQBnALFbVL6YEXqHwpEoe0Yye262O14k-rSlzgHM06Y-rKSbZ2AsqNd-ANeZJ62ZXgwsmH-Wm-8cfYGwGXAkBehUd3KUEa-YKtBFhTgRTqiK3KDCojan3CXuX8CCCEguaYnQglpbDGrBjdxIkSTmEcMg8Dn-Z-THwgl8YcMvfopjH9_vkrDN3sqOM0dOP0QDFg5I5i5Ns0uhG_zRGHiRcdvodp4ZuFPyxbSoWmPuBr9tJjzHR2eE_Z1w_vv6w_VfefP96ub-4rVyshK20EkZWdJw0EVgphG2x8QxtStW9qQ96hVppsB-raWUBUHrC24LTRXVefsrf7veWqp5ny1PYh787EgcY5t1prZayUBb7bw90_cyLfblPoMS2tgHbXaVs6bf90Wuz5Yem86an7Kw8lFnBxAJgdRp9wcCH_4-x1id25q737ESIt_09sb-_W--hnglKQhw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>66657922</pqid></control><display><type>article</type><title>Alterations in tumor necrosis factor–induced endothelial cell procoagulant activity by hyperthermia</title><source>MEDLINE</source><source>Access via Wiley Online Library</source><source>EZB-FREE-00999 freely available EZB journals</source><creator>Carroll, Nancy M. ; Elaraj, Dina M. ; Puhlmann, Markus ; Weinreich, David M. ; Turner, Ewa M. ; Xu, Hui ; Alexander, H. Richard</creator><creatorcontrib>Carroll, Nancy M. ; Elaraj, Dina M. ; Puhlmann, Markus ; Weinreich, David M. ; Turner, Ewa M. ; Xu, Hui ; Alexander, H. Richard</creatorcontrib><description>TNF is a cytokine with potent antitumor activity in murine models and when administered clinically via regional perfusion. There is substantial evidence that this antitumor activity depends in large part on TNF's procoagulant effect on tumor neovasculature, which is mediated by induction of endothelial cell tissue factor (TF), a component of the extrinsic clotting cascade. In regional perfusion of a cancer‐bearing limb or organ, TNF is always administered under hyperthermic temperatures; however, little is known about the effect of hyperthermia on TNF‐mediated procoagulant activity in endothelium. We examined the effects of hyperthermia on TNF‐mediated procoagulant activity in human umbilical vein endothelial cells (HUVECs). HUVECs were exposed to TNF at normothermic (37°C) and hyperthermic (41°C) temperatures for 90 min, then assayed for clotting activity, TF protein production and mRNA production of TF and tissue factor pathway inhibitor‐2 (TFPI‐2), an endogenous inducible inhibitor of TF activity in HUVECs. TNF treatment at 41°C significantly reduced clotting activity, TF protein and mRNA as well as TFPI‐2 mRNA compared to treatment at 37°C. These data show that hyperthermia significantly reduces the procoagulant effects of TNF on endothelial tissue compared to normothermia, which may have important clinical implications for the use of TNF in regional perfusion. © 2004 Wiley‐Liss, Inc.</description><identifier>ISSN: 0020-7136</identifier><identifier>EISSN: 1097-0215</identifier><identifier>DOI: 10.1002/ijc.20272</identifier><identifier>PMID: 15221977</identifier><identifier>CODEN: IJCNAW</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Biological and medical sciences ; Blood Coagulation Factors - genetics ; Cell Line ; coagulation ; endothelium ; Endothelium, Vascular - drug effects ; Endothelium, Vascular - physiology ; Gene Expression Regulation - drug effects ; Glycoproteins - genetics ; Hot Temperature ; Humans ; hyperthermia ; Hyperthermia, Induced ; Induced hyperthermia. Cryotherapy ; isolated limb perfusion ; Kinetics ; Medical sciences ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Messenger - genetics ; Thromboplastin - genetics ; tissue factor ; tissue factor pathway inhibitor‐2 ; Transcription, Genetic - drug effects ; Treatment with physical agents ; Treatment. General aspects ; tumor necrosis factor ; Tumor Necrosis Factor-alpha - pharmacology ; Tumors ; Umbilical Veins</subject><ispartof>International journal of cancer, 2004-09, Vol.111 (3), p.457-462</ispartof><rights>Copyright © 2004 Wiley‐Liss, Inc.</rights><rights>2004 INIST-CNRS</rights><rights>Copyright 2004 Wiley-Liss, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3512-671ee92dfe60e0921194a4f4ebe53f437efca656e9d058c90aa5f0a390c676dd3</citedby><cites>FETCH-LOGICAL-c3512-671ee92dfe60e0921194a4f4ebe53f437efca656e9d058c90aa5f0a390c676dd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fijc.20272$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fijc.20272$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15986577$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15221977$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Carroll, Nancy M.</creatorcontrib><creatorcontrib>Elaraj, Dina M.</creatorcontrib><creatorcontrib>Puhlmann, Markus</creatorcontrib><creatorcontrib>Weinreich, David M.</creatorcontrib><creatorcontrib>Turner, Ewa M.</creatorcontrib><creatorcontrib>Xu, Hui</creatorcontrib><creatorcontrib>Alexander, H. Richard</creatorcontrib><title>Alterations in tumor necrosis factor–induced endothelial cell procoagulant activity by hyperthermia</title><title>International journal of cancer</title><addtitle>Int J Cancer</addtitle><description>TNF is a cytokine with potent antitumor activity in murine models and when administered clinically via regional perfusion. There is substantial evidence that this antitumor activity depends in large part on TNF's procoagulant effect on tumor neovasculature, which is mediated by induction of endothelial cell tissue factor (TF), a component of the extrinsic clotting cascade. In regional perfusion of a cancer‐bearing limb or organ, TNF is always administered under hyperthermic temperatures; however, little is known about the effect of hyperthermia on TNF‐mediated procoagulant activity in endothelium. We examined the effects of hyperthermia on TNF‐mediated procoagulant activity in human umbilical vein endothelial cells (HUVECs). HUVECs were exposed to TNF at normothermic (37°C) and hyperthermic (41°C) temperatures for 90 min, then assayed for clotting activity, TF protein production and mRNA production of TF and tissue factor pathway inhibitor‐2 (TFPI‐2), an endogenous inducible inhibitor of TF activity in HUVECs. TNF treatment at 41°C significantly reduced clotting activity, TF protein and mRNA as well as TFPI‐2 mRNA compared to treatment at 37°C. These data show that hyperthermia significantly reduces the procoagulant effects of TNF on endothelial tissue compared to normothermia, which may have important clinical implications for the use of TNF in regional perfusion. © 2004 Wiley‐Liss, Inc.</description><subject>Biological and medical sciences</subject><subject>Blood Coagulation Factors - genetics</subject><subject>Cell Line</subject><subject>coagulation</subject><subject>endothelium</subject><subject>Endothelium, Vascular - drug effects</subject><subject>Endothelium, Vascular - physiology</subject><subject>Gene Expression Regulation - drug effects</subject><subject>Glycoproteins - genetics</subject><subject>Hot Temperature</subject><subject>Humans</subject><subject>hyperthermia</subject><subject>Hyperthermia, Induced</subject><subject>Induced hyperthermia. Cryotherapy</subject><subject>isolated limb perfusion</subject><subject>Kinetics</subject><subject>Medical sciences</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Messenger - genetics</subject><subject>Thromboplastin - genetics</subject><subject>tissue factor</subject><subject>tissue factor pathway inhibitor‐2</subject><subject>Transcription, Genetic - drug effects</subject><subject>Treatment with physical agents</subject><subject>Treatment. General aspects</subject><subject>tumor necrosis factor</subject><subject>Tumor Necrosis Factor-alpha - pharmacology</subject><subject>Tumors</subject><subject>Umbilical Veins</subject><issn>0020-7136</issn><issn>1097-0215</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp10M9u1DAQBnALFbVL6YEXqHwpEoe0Yye262O14k-rSlzgHM06Y-rKSbZ2AsqNd-ANeZJ62ZXgwsmH-Wm-8cfYGwGXAkBehUd3KUEa-YKtBFhTgRTqiK3KDCojan3CXuX8CCCEguaYnQglpbDGrBjdxIkSTmEcMg8Dn-Z-THwgl8YcMvfopjH9_vkrDN3sqOM0dOP0QDFg5I5i5Ns0uhG_zRGHiRcdvodp4ZuFPyxbSoWmPuBr9tJjzHR2eE_Z1w_vv6w_VfefP96ub-4rVyshK20EkZWdJw0EVgphG2x8QxtStW9qQ96hVppsB-raWUBUHrC24LTRXVefsrf7veWqp5ny1PYh787EgcY5t1prZayUBb7bw90_cyLfblPoMS2tgHbXaVs6bf90Wuz5Yem86an7Kw8lFnBxAJgdRp9wcCH_4-x1id25q737ESIt_09sb-_W--hnglKQhw</recordid><startdate>20040901</startdate><enddate>20040901</enddate><creator>Carroll, Nancy M.</creator><creator>Elaraj, Dina M.</creator><creator>Puhlmann, Markus</creator><creator>Weinreich, David M.</creator><creator>Turner, Ewa M.</creator><creator>Xu, Hui</creator><creator>Alexander, H. Richard</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley-Liss</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20040901</creationdate><title>Alterations in tumor necrosis factor–induced endothelial cell procoagulant activity by hyperthermia</title><author>Carroll, Nancy M. ; Elaraj, Dina M. ; Puhlmann, Markus ; Weinreich, David M. ; Turner, Ewa M. ; Xu, Hui ; Alexander, H. Richard</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3512-671ee92dfe60e0921194a4f4ebe53f437efca656e9d058c90aa5f0a390c676dd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Biological and medical sciences</topic><topic>Blood Coagulation Factors - genetics</topic><topic>Cell Line</topic><topic>coagulation</topic><topic>endothelium</topic><topic>Endothelium, Vascular - drug effects</topic><topic>Endothelium, Vascular - physiology</topic><topic>Gene Expression Regulation - drug effects</topic><topic>Glycoproteins - genetics</topic><topic>Hot Temperature</topic><topic>Humans</topic><topic>hyperthermia</topic><topic>Hyperthermia, Induced</topic><topic>Induced hyperthermia. Cryotherapy</topic><topic>isolated limb perfusion</topic><topic>Kinetics</topic><topic>Medical sciences</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Messenger - genetics</topic><topic>Thromboplastin - genetics</topic><topic>tissue factor</topic><topic>tissue factor pathway inhibitor‐2</topic><topic>Transcription, Genetic - drug effects</topic><topic>Treatment with physical agents</topic><topic>Treatment. General aspects</topic><topic>tumor necrosis factor</topic><topic>Tumor Necrosis Factor-alpha - pharmacology</topic><topic>Tumors</topic><topic>Umbilical Veins</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Carroll, Nancy M.</creatorcontrib><creatorcontrib>Elaraj, Dina M.</creatorcontrib><creatorcontrib>Puhlmann, Markus</creatorcontrib><creatorcontrib>Weinreich, David M.</creatorcontrib><creatorcontrib>Turner, Ewa M.</creatorcontrib><creatorcontrib>Xu, Hui</creatorcontrib><creatorcontrib>Alexander, H. Richard</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of cancer</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Carroll, Nancy M.</au><au>Elaraj, Dina M.</au><au>Puhlmann, Markus</au><au>Weinreich, David M.</au><au>Turner, Ewa M.</au><au>Xu, Hui</au><au>Alexander, H. Richard</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Alterations in tumor necrosis factor–induced endothelial cell procoagulant activity by hyperthermia</atitle><jtitle>International journal of cancer</jtitle><addtitle>Int J Cancer</addtitle><date>2004-09-01</date><risdate>2004</risdate><volume>111</volume><issue>3</issue><spage>457</spage><epage>462</epage><pages>457-462</pages><issn>0020-7136</issn><eissn>1097-0215</eissn><coden>IJCNAW</coden><abstract>TNF is a cytokine with potent antitumor activity in murine models and when administered clinically via regional perfusion. There is substantial evidence that this antitumor activity depends in large part on TNF's procoagulant effect on tumor neovasculature, which is mediated by induction of endothelial cell tissue factor (TF), a component of the extrinsic clotting cascade. In regional perfusion of a cancer‐bearing limb or organ, TNF is always administered under hyperthermic temperatures; however, little is known about the effect of hyperthermia on TNF‐mediated procoagulant activity in endothelium. We examined the effects of hyperthermia on TNF‐mediated procoagulant activity in human umbilical vein endothelial cells (HUVECs). HUVECs were exposed to TNF at normothermic (37°C) and hyperthermic (41°C) temperatures for 90 min, then assayed for clotting activity, TF protein production and mRNA production of TF and tissue factor pathway inhibitor‐2 (TFPI‐2), an endogenous inducible inhibitor of TF activity in HUVECs. TNF treatment at 41°C significantly reduced clotting activity, TF protein and mRNA as well as TFPI‐2 mRNA compared to treatment at 37°C. These data show that hyperthermia significantly reduces the procoagulant effects of TNF on endothelial tissue compared to normothermia, which may have important clinical implications for the use of TNF in regional perfusion. © 2004 Wiley‐Liss, Inc.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>15221977</pmid><doi>10.1002/ijc.20272</doi><tpages>6</tpages></addata></record> |
fulltext | fulltext |
identifier | ISSN: 0020-7136 |
ispartof | International journal of cancer, 2004-09, Vol.111 (3), p.457-462 |
issn | 0020-7136 1097-0215 |
language | eng |
recordid | cdi_proquest_miscellaneous_66657922 |
source | MEDLINE; Access via Wiley Online Library; EZB-FREE-00999 freely available EZB journals |
subjects | Biological and medical sciences Blood Coagulation Factors - genetics Cell Line coagulation endothelium Endothelium, Vascular - drug effects Endothelium, Vascular - physiology Gene Expression Regulation - drug effects Glycoproteins - genetics Hot Temperature Humans hyperthermia Hyperthermia, Induced Induced hyperthermia. Cryotherapy isolated limb perfusion Kinetics Medical sciences Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - genetics Thromboplastin - genetics tissue factor tissue factor pathway inhibitor‐2 Transcription, Genetic - drug effects Treatment with physical agents Treatment. General aspects tumor necrosis factor Tumor Necrosis Factor-alpha - pharmacology Tumors Umbilical Veins |
title | Alterations in tumor necrosis factor–induced endothelial cell procoagulant activity by hyperthermia |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-20T08%3A26%3A50IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Alterations%20in%20tumor%20necrosis%20factor%E2%80%93induced%20endothelial%20cell%20procoagulant%20activity%20by%20hyperthermia&rft.jtitle=International%20journal%20of%20cancer&rft.au=Carroll,%20Nancy%20M.&rft.date=2004-09-01&rft.volume=111&rft.issue=3&rft.spage=457&rft.epage=462&rft.pages=457-462&rft.issn=0020-7136&rft.eissn=1097-0215&rft.coden=IJCNAW&rft_id=info:doi/10.1002/ijc.20272&rft_dat=%3Cproquest_cross%3E66657922%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=66657922&rft_id=info:pmid/15221977&rfr_iscdi=true |