In vitro hydroxyapatite adsorbed salivary proteins
In spite of the present knowledge about saliva components and their respective functions, the mechanism(s) of pellicle and dental plaque formation have hitherto remained obscure. This has prompted recent efforts on in vitro studies using hydroxyapatite (HA) as an enamel model. In the present study s...
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| Veröffentlicht in: | Biochemical and biophysical research communications 2004-07, Vol.320 (2), p.342-346 |
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| container_title | Biochemical and biophysical research communications |
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| creator | Vitorino, Rui Lobo, Maria João C Duarte, José Ferrer-Correia, António J Tomer, Kenneth B Dubin, Joshua R Domingues, Pedro M Amado, Francisco M.L |
| description | In spite of the present knowledge about saliva components and their respective functions, the mechanism(s) of pellicle and dental plaque formation have hitherto remained obscure. This has prompted recent efforts on in vitro studies using hydroxyapatite (HA) as an enamel model. In the present study salivary proteins adsorbed to HA were extracted with TFA and EDTA and resolved by 2D electrophoresis over a pH range between 3 and 10, digested, and then analysed by MALDI-TOF/TOF mass spectrometry and tandem mass spectrometry. Nineteen different proteins were identified using automated MS and MS/MS data acquisition. Among them, cystatins, amylase, carbonic anhydrase, and calgranulin B, were identified. |
| doi_str_mv | 10.1016/j.bbrc.2004.05.169 |
| format | Article |
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This has prompted recent efforts on in vitro studies using hydroxyapatite (HA) as an enamel model. In the present study salivary proteins adsorbed to HA were extracted with TFA and EDTA and resolved by 2D electrophoresis over a pH range between 3 and 10, digested, and then analysed by MALDI-TOF/TOF mass spectrometry and tandem mass spectrometry. Nineteen different proteins were identified using automated MS and MS/MS data acquisition. 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| ispartof | Biochemical and biophysical research communications, 2004-07, Vol.320 (2), p.342-346 |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Durapatite - chemistry Electrophoresis, Gel, Two-Dimensional Humans Hydroxyapatite In Vitro Techniques MALDI-TOF/TOF Male Peptide Mapping Saliva Salivary Proteins and Peptides - chemistry Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Trypsin Two-dimensional electrophoresis |
| title | In vitro hydroxyapatite adsorbed salivary proteins |
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