Molecular characterization of a novel amastigote stage specific Class I nuclease from Leishmania major

Leishmania parasites like other kinetoplastids are unable to synthesize purines de novo and so are reliant on a salvage pathway for recycling ribonucleotides. A stage specific class one nuclease enzyme, 3′-Nucleotidase/nuclease, has been implicated in salvage of preformed purines in Leishmania insec...

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Veröffentlicht in:	International journal for parasitology 2004-07, Vol.34 (8), p.899-908
Hauptverfasser:	Farajnia, S, Alimohammadian, M.H, Reiner, N.E, Karimi, M, Ajdari, S, Mahboudi, F
Format:	Artikel
Sprache:	eng
Schlagworte:	3′-Nnucleotidase/nuclease Amastigote Amino Acid Sequence Animals Base Sequence Biological and medical sciences Blotting, Southern - methods Class I nuclease Cloning, Molecular - methods Culture Media Fundamental and applied biological sciences. Psychology Gene Expression Regulation - genetics Leishmania major Leishmania major - enzymology Leishmania major - genetics Life cycle. Host-agent relationship. Pathogenesis Molecular Sequence Data Nucleotidases - genetics Nucleotidases - metabolism Protozoa Protozoan Proteins - genetics Purine metabolism Purine salvage Purines - metabolism Recombinant Proteins - genetics Reverse Transcriptase Polymerase Chain Reaction - methods Sequence Alignment
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