Identification and Characterization of Two Contiguous Operons Required for Aerobactin Transport and Biosynthesis in Vibrio mimicus
In response to iron deprivation, Vibrio mimicus produces aerobactin as a major siderophore. Application of the Fur titration assay to a V. mimicus genomic DNA library followed by further cloning of the surrounding regions led to the identification of two adjacent, iron‐regulated operons. One contain...
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| Veröffentlicht in: | Microbiology and immunology 2004-01, Vol.48 (5), p.389-398 |
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| creator | Moon, Yong-Hwa Tanabe, Tomotaka Funahashi, Tatsuya Shiuchi, Kei-ichi Nakao, Hiroshi Yamamoto, Shigeo |
| description | In response to iron deprivation, Vibrio mimicus produces aerobactin as a major siderophore. Application of the Fur titration assay to a V. mimicus genomic DNA library followed by further cloning of the surrounding regions led to the identification of two adjacent, iron‐regulated operons. One contains three genes encoding homologs of the Escherichia coli FhuCDB and the other, five genes encoding homologs of the E. coli IucABCD IutA. Construction of the V. mimicus polar disruptants in the respective operons allowed us to confirm their functions. The genetic arrangement of the aerobactin‐mediated iron acquisition system in V. mimicus is unique in that the aerobactin operon (iucABCD iutA) is contiguous to the operon (matCDB) encoding components of an ATP‐binding cassette transport system for ferric aerobactin. This is the first report demonstrating that aerobactin transport and biosynthesis genes are present in a species outside the family Enterobacteriaceae. |
| doi_str_mv | 10.1111/j.1348-0421.2004.tb03528.x |
| format | Article |
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Application of the Fur titration assay to a V. mimicus genomic DNA library followed by further cloning of the surrounding regions led to the identification of two adjacent, iron‐regulated operons. One contains three genes encoding homologs of the Escherichia coli FhuCDB and the other, five genes encoding homologs of the E. coli IucABCD IutA. Construction of the V. mimicus polar disruptants in the respective operons allowed us to confirm their functions. The genetic arrangement of the aerobactin‐mediated iron acquisition system in V. mimicus is unique in that the aerobactin operon (iucABCD iutA) is contiguous to the operon (matCDB) encoding components of an ATP‐binding cassette transport system for ferric aerobactin. 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This is the first report demonstrating that aerobactin transport and biosynthesis genes are present in a species outside the family Enterobacteriaceae.</description><subject>ABC transport system</subject><subject>aerobactin operon</subject><subject>ATP-Binding Cassette Transporters - genetics</subject><subject>ATP-Binding Cassette Transporters - physiology</subject><subject>Bacterial Outer Membrane Proteins - genetics</subject><subject>Biological Transport, Active - genetics</subject><subject>Carrier Proteins - genetics</subject><subject>DNA, Bacterial - chemistry</subject><subject>DNA, Bacterial - isolation & purification</subject><subject>Escherichia coli</subject><subject>Escherichia coli - genetics</subject><subject>Escherichia coli Proteins - genetics</subject><subject>Gene Order</subject><subject>Genes, Bacterial</subject><subject>Genomic Library</subject><subject>Hydroxamic Acids - metabolism</subject><subject>Iron - metabolism</subject><subject>iron uptake</subject><subject>iron-regulated gene</subject><subject>Membrane Transport Proteins - genetics</subject><subject>Molecular Sequence Data</subject><subject>Mutation</subject><subject>Operon</subject><subject>Periplasmic Binding Proteins - genetics</subject><subject>Promoter Regions, Genetic</subject><subject>Sequence Analysis, DNA</subject><subject>Sequence Homology</subject><subject>Siderophores - biosynthesis</subject><subject>Siderophores - genetics</subject><subject>Siderophores - physiology</subject><subject>Vibrio mimicus</subject><subject>Vibrio mimicus - genetics</subject><subject>Vibrio mimicus - metabolism</subject><issn>0385-5600</issn><issn>1348-0421</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVkUtv3CAUhVHUKpmm-QsR6qI7uzwMxl01GbXpRHkpmiRLhDE0TMdmArYy02V_eZl4lC6rsgHde865unwAfMAox-l8WuSYFiJDBcE5QajI-xpRRkS-3gOT19YbMEFUsIxxhA7AuxgXCJGSiGIfHGBGMOOET8DvWWO63lmnVe98B1XXwOmjCkr3JrhfY9FbOH_2cOqT8sfghwivVyb4LsJb8zS4YBpofYAnqVYno-vgPKgurnzoXwJPnY-brn800UWYuveuDs7D1rVOD_E9eGvVMpqj3X0I7r59nU-_ZxfXZ7PpyUWmORIiY0JhVQlea15RXVGjRWmJJoRR1Fiqa8swSruWBcfKWso0pQIjgWpOcSEKegg-jrmr4J8GE3vZuqjNcqk6k3aSnHOG06f8U4jLSlBaVkn4eRTq4GMMxspVcK0KG4mR3KKSC7nlIbc85BaV3KGS62Q-3k0Z6tY0f607NknwZRQ8u6XZ_Ee0vJxdvjxTRDZGuNib9WuECj8lL2nJ5MPVmSTn55he3SB5T_8Awsq0hg</recordid><startdate>20040101</startdate><enddate>20040101</enddate><creator>Moon, Yong-Hwa</creator><creator>Tanabe, Tomotaka</creator><creator>Funahashi, Tatsuya</creator><creator>Shiuchi, Kei-ichi</creator><creator>Nakao, Hiroshi</creator><creator>Yamamoto, Shigeo</creator><general>Blackwell Publishing Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>20040101</creationdate><title>Identification and Characterization of Two Contiguous Operons Required for Aerobactin Transport and Biosynthesis in Vibrio mimicus</title><author>Moon, Yong-Hwa ; Tanabe, Tomotaka ; Funahashi, Tatsuya ; Shiuchi, Kei-ichi ; Nakao, Hiroshi ; Yamamoto, Shigeo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c6088-58a1a986bc693c93ec87f2c22530df3cbf5106007461aff35c3381080b6314843</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>ABC transport system</topic><topic>aerobactin operon</topic><topic>ATP-Binding Cassette Transporters - genetics</topic><topic>ATP-Binding Cassette Transporters - physiology</topic><topic>Bacterial Outer Membrane Proteins - genetics</topic><topic>Biological Transport, Active - genetics</topic><topic>Carrier Proteins - genetics</topic><topic>DNA, Bacterial - chemistry</topic><topic>DNA, Bacterial - isolation & purification</topic><topic>Escherichia coli</topic><topic>Escherichia coli - genetics</topic><topic>Escherichia coli Proteins - genetics</topic><topic>Gene Order</topic><topic>Genes, Bacterial</topic><topic>Genomic Library</topic><topic>Hydroxamic Acids - metabolism</topic><topic>Iron - metabolism</topic><topic>iron uptake</topic><topic>iron-regulated gene</topic><topic>Membrane Transport Proteins - genetics</topic><topic>Molecular Sequence Data</topic><topic>Mutation</topic><topic>Operon</topic><topic>Periplasmic Binding Proteins - genetics</topic><topic>Promoter Regions, Genetic</topic><topic>Sequence Analysis, DNA</topic><topic>Sequence Homology</topic><topic>Siderophores - biosynthesis</topic><topic>Siderophores - genetics</topic><topic>Siderophores - physiology</topic><topic>Vibrio mimicus</topic><topic>Vibrio mimicus - genetics</topic><topic>Vibrio mimicus - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Moon, Yong-Hwa</creatorcontrib><creatorcontrib>Tanabe, Tomotaka</creatorcontrib><creatorcontrib>Funahashi, Tatsuya</creatorcontrib><creatorcontrib>Shiuchi, Kei-ichi</creatorcontrib><creatorcontrib>Nakao, Hiroshi</creatorcontrib><creatorcontrib>Yamamoto, Shigeo</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>Microbiology and immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Moon, Yong-Hwa</au><au>Tanabe, Tomotaka</au><au>Funahashi, Tatsuya</au><au>Shiuchi, Kei-ichi</au><au>Nakao, Hiroshi</au><au>Yamamoto, Shigeo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification and Characterization of Two Contiguous Operons Required for Aerobactin Transport and Biosynthesis in Vibrio mimicus</atitle><jtitle>Microbiology and immunology</jtitle><addtitle>Microbiology and Immunology</addtitle><date>2004-01-01</date><risdate>2004</risdate><volume>48</volume><issue>5</issue><spage>389</spage><epage>398</epage><pages>389-398</pages><issn>0385-5600</issn><eissn>1348-0421</eissn><abstract>In response to iron deprivation, Vibrio mimicus produces aerobactin as a major siderophore. Application of the Fur titration assay to a V. mimicus genomic DNA library followed by further cloning of the surrounding regions led to the identification of two adjacent, iron‐regulated operons. One contains three genes encoding homologs of the Escherichia coli FhuCDB and the other, five genes encoding homologs of the E. coli IucABCD IutA. Construction of the V. mimicus polar disruptants in the respective operons allowed us to confirm their functions. The genetic arrangement of the aerobactin‐mediated iron acquisition system in V. mimicus is unique in that the aerobactin operon (iucABCD iutA) is contiguous to the operon (matCDB) encoding components of an ATP‐binding cassette transport system for ferric aerobactin. 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| source | MEDLINE; Wiley Free Content; Open Access Titles of Japan; Wiley Online Library All Journals; Alma/SFX Local Collection |
| subjects | ABC transport system aerobactin operon ATP-Binding Cassette Transporters - genetics ATP-Binding Cassette Transporters - physiology Bacterial Outer Membrane Proteins - genetics Biological Transport, Active - genetics Carrier Proteins - genetics DNA, Bacterial - chemistry DNA, Bacterial - isolation & purification Escherichia coli Escherichia coli - genetics Escherichia coli Proteins - genetics Gene Order Genes, Bacterial Genomic Library Hydroxamic Acids - metabolism Iron - metabolism iron uptake iron-regulated gene Membrane Transport Proteins - genetics Molecular Sequence Data Mutation Operon Periplasmic Binding Proteins - genetics Promoter Regions, Genetic Sequence Analysis, DNA Sequence Homology Siderophores - biosynthesis Siderophores - genetics Siderophores - physiology Vibrio mimicus Vibrio mimicus - genetics Vibrio mimicus - metabolism |
| title | Identification and Characterization of Two Contiguous Operons Required for Aerobactin Transport and Biosynthesis in Vibrio mimicus |
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