Exposure to Aspergillus fumigatus spores induces chemokine expression in mouse macrophages

Inhalation of fungal spores may cause inflammation and respiratory diseases, such as bronchitis, allergic alveolitis, and asthma. Alveolar macrophages provide the first line of defense in the respiratory tract. To examine the cellular mechanisms involved in Aspergillus fumigatus-induced airway infla...

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Veröffentlicht in:Toxicology (Amsterdam) 2004-08, Vol.200 (2-3), p.255-263
Hauptverfasser: Pylkkänen, Lea, Gullstén, Harriet, Majuri, Marja-Leena, Andersson, Ulpu, Vanhala, Esa, Määttä, Juha, Meklin, Teija, Hirvonen, Maija-Riitta, Alenius, Harri, Savolainen, Kai
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container_title Toxicology (Amsterdam)
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creator Pylkkänen, Lea
Gullstén, Harriet
Majuri, Marja-Leena
Andersson, Ulpu
Vanhala, Esa
Määttä, Juha
Meklin, Teija
Hirvonen, Maija-Riitta
Alenius, Harri
Savolainen, Kai
description Inhalation of fungal spores may cause inflammation and respiratory diseases, such as bronchitis, allergic alveolitis, and asthma. Alveolar macrophages provide the first line of defense in the respiratory tract. To examine the cellular mechanisms involved in Aspergillus fumigatus-induced airway inflammation, mouse macrophage cell line (RAW 264.7) cells were exposed for 2h or 6h to graded doses of A. fumigatus spores that were either alive or heat-killed. Furthermore, the ability of the cells to phagocytize the spores was visualized by electron microscopy. Expression of selected cytokines and chemokines was assessed by a real time quantitative PCR method and by enzyme-linked immunoabsorbent assay (ELISA) after exposure. A significant increase in mRNA expression of TNF-α, MIP-1α, MIP-1β, and MCP-1 was observed with a maximal induction at 6h after exposure to the highest (1×107) concentration of live spores. Similar response was not detected with heat-killed spores in the expression of chemokines and cytokines, even though there were no differences between the phagocytosis of live and heat-killed spores. These results suggest that exposure to live spores of A. fumigatus can modulate the expression of proinflammatory cytokines and chemokines in mouse macrophages and thus influence the development of inflammatory processes in the airways.
doi_str_mv 10.1016/j.tox.2004.03.019
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subjects Animals
Aspergillus fumigatus
Biological and medical sciences
Cell Line
Chemokine
Chemokine CCL2 - biosynthesis
Chemokines - biosynthesis
Cytokine
Cytokines - biosynthesis
DNA, Complementary - biosynthesis
DNA, Complementary - genetics
Enzyme-Linked Immunosorbent Assay
Interleukin-6 - biosynthesis
Macrophages - metabolism
Medical sciences
Mice
Microscopy, Electron
Mouse macrophage
Phagocytosis
Phagocytosis - physiology
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - biosynthesis
Spores, Fungal
Toxicology
Tumor Necrosis Factor-alpha - biosynthesis
title Exposure to Aspergillus fumigatus spores induces chemokine expression in mouse macrophages
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