Estrogen receptor β localization in the lizard (Podarcis s. sicula) testis
There is increasing evidence that 17β-estradiol is necessary for normal male fertility. The aim of the present study was to characterize estrogen receptor β (ERβ) expression in a non-mammalian vertebrate model, the lizard (Podarcis s. sicula) testis. Immunocytochemical analysis shows that ERβ protei...
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| Veröffentlicht in: | Zygote (Cambridge) 2004-02, Vol.12 (1), p.39-42 |
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| creator | Chieffi, Paolo Varriale, Bruno |
| description | There is increasing evidence that 17β-estradiol is necessary for normal male fertility. The aim of the present study was to characterize estrogen receptor β (ERβ) expression in a non-mammalian vertebrate model, the lizard (Podarcis s. sicula) testis. Immunocytochemical analysis shows that ERβ proteins are present among germ cells in the nucleus of the spermatogonia, in primary spermatocytes and spermatids. Western blot analysis with antibodies against the ERβ gene product revealed an isoform with a specific weight of 55 kDa. In conclusion, the widespread expression of ERβ in the Podarcis s. sicula testis is consistent with a role for estrogens in modulating spermatogenesis in the male. |
| doi_str_mv | 10.1017/S0967199404002618 |
| format | Article |
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| language | eng |
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| source | MEDLINE; Cambridge University Press Journals Complete |
| subjects | Animals Blotting, Western Estrogen receptor Estrogen Receptor beta - metabolism Fertility Immunohistochemistry Lacertilia Lizards Male Podarcis Proliferation Spermatogenesis Testis Testis - chemistry Testis - cytology |
| title | Estrogen receptor β localization in the lizard (Podarcis s. sicula) testis |
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