Genetic Differences Between Culex pipiens f. molestus and Culex pipiens pipiens (Diptera: Culicidae) in New York
The definition and phylogenetic placement of the autogenous molestus form of Culex pipiens has puzzled entomologists for decades. We identified genetic differences between Cx. p. pipiens (L.) and Cx. pipiens f. molestus Forskaal in the SH60 fragment described previously. Single-strand conformation p...
Gespeichert in:
| Veröffentlicht in: | Journal of medical entomology 2007-01, Vol.44 (1), p.50-59 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 59 |
|---|---|
| container_issue | 1 |
| container_start_page | 50 |
| container_title | Journal of medical entomology |
| container_volume | 44 |
| creator | Kent, Rebekah J. Harrington, Laura C. Norris, Douglas E. |
| description | The definition and phylogenetic placement of the autogenous molestus form of Culex pipiens has puzzled entomologists for decades. We identified genetic differences between Cx. p. pipiens (L.) and Cx. pipiens f. molestus Forskaal in the SH60 fragment described previously. Single-strand conformation polymorphism analysis, cloning, and sequencing of this fragment demonstrated high polymorphism within and among individual Cx. p. pipiens, with common SH60 variants shared among individuals from distant locations. In contrast, Cx. pipiens f. molestus from New York City each contained a single SH60 variant, which was not identified in any other Cx. p. pipiens specimens analyzed. Supporting microsatellite analysis demonstrated significant but reduced gene flow between Cx. p. pipiens and Cx. pipiens f. molestus in New York relative to Cx. p. pipiens populations in New York and California. Results are discussed in the context of two contrasting hypotheses regarding the origin of Cx. pipiens f. molestus populations. |
| doi_str_mv | 10.1603/0022-2585(2007)44[50:GDBCPF]2.0.CO;2 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_47322632</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>47322632</sourcerecordid><originalsourceid>FETCH-LOGICAL-c407t-a028b5cdeaffc400103d2e2e9d4385ce07f103e80e5891b9e8ff3db2bf89587d3</originalsourceid><addsrcrecordid>eNqFUcFKw0AUDKJgrf7DnqQFG182u82mnmxiq1CsBz2IyJJs3sJqmsRsQvXvTaiKePE0j5nhvceM45x54HpT8M8BKJ1QLviIAgRjxp44zJbxPLpbPFMX3Gh9QfecwY9t_9d86BxZ-wIAwmPhwKmWWGBjFImN1lhjodCSOTZbxIJEbY7vpDKVwcIS7ZJNmaNtWkuSIvujfuMoNlWDdTLrdaNMluCYmILc4pY8lvXrsXOgk9ziyRcOnYfF1X10PVmtlzfR5WqiGATNJAEqUq4yTLTuGPDAzyhSDDPmC64QAt1RKAC5CL00RKG1n6U01SLkIsj8oXO621vV5VvbfS03xirM86TAsrWSBT6lU5_-a6TApl53szPGO6OqS2tr1LKqzSapP6QHsu9F9iHLPmTZ9yIZkxzkrhdJJchoLan_CaQyhGU</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>20461385</pqid></control><display><type>article</type><title>Genetic Differences Between Culex pipiens f. molestus and Culex pipiens pipiens (Diptera: Culicidae) in New York</title><source>Oxford University Press Journals All Titles (1996-Current)</source><source>BioOne Complete</source><creator>Kent, Rebekah J. ; Harrington, Laura C. ; Norris, Douglas E.</creator><creatorcontrib>Kent, Rebekah J. ; Harrington, Laura C. ; Norris, Douglas E.</creatorcontrib><description>The definition and phylogenetic placement of the autogenous molestus form of Culex pipiens has puzzled entomologists for decades. We identified genetic differences between Cx. p. pipiens (L.) and Cx. pipiens f. molestus Forskaal in the SH60 fragment described previously. Single-strand conformation polymorphism analysis, cloning, and sequencing of this fragment demonstrated high polymorphism within and among individual Cx. p. pipiens, with common SH60 variants shared among individuals from distant locations. In contrast, Cx. pipiens f. molestus from New York City each contained a single SH60 variant, which was not identified in any other Cx. p. pipiens specimens analyzed. Supporting microsatellite analysis demonstrated significant but reduced gene flow between Cx. p. pipiens and Cx. pipiens f. molestus in New York relative to Cx. p. pipiens populations in New York and California. Results are discussed in the context of two contrasting hypotheses regarding the origin of Cx. pipiens f. molestus populations.</description><identifier>ISSN: 0022-2585</identifier><identifier>EISSN: 0022-2585</identifier><identifier>DOI: 10.1603/0022-2585(2007)44[50:GDBCPF]2.0.CO;2</identifier><language>eng</language><subject>Animals ; Base Sequence ; Culex ; Culex pipiens ; Culex pipiens form molestus ; Culex pipiens pipiens ; Culicidae ; Diptera ; gene flow ; genetic markers ; genetic variation ; genetics ; geographical distribution ; Insect Vectors ; microsatellite repeats ; mitochondrial DNA ; Molecular Sequence Data ; New York ; restriction fragment length polymorphism ; ribosomal DNA ; Sequence Alignment ; single-stranded conformational polymorphism</subject><ispartof>Journal of medical entomology, 2007-01, Vol.44 (1), p.50-59</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c407t-a028b5cdeaffc400103d2e2e9d4385ce07f103e80e5891b9e8ff3db2bf89587d3</citedby><cites>FETCH-LOGICAL-c407t-a028b5cdeaffc400103d2e2e9d4385ce07f103e80e5891b9e8ff3db2bf89587d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids></links><search><creatorcontrib>Kent, Rebekah J.</creatorcontrib><creatorcontrib>Harrington, Laura C.</creatorcontrib><creatorcontrib>Norris, Douglas E.</creatorcontrib><title>Genetic Differences Between Culex pipiens f. molestus and Culex pipiens pipiens (Diptera: Culicidae) in New York</title><title>Journal of medical entomology</title><description>The definition and phylogenetic placement of the autogenous molestus form of Culex pipiens has puzzled entomologists for decades. We identified genetic differences between Cx. p. pipiens (L.) and Cx. pipiens f. molestus Forskaal in the SH60 fragment described previously. Single-strand conformation polymorphism analysis, cloning, and sequencing of this fragment demonstrated high polymorphism within and among individual Cx. p. pipiens, with common SH60 variants shared among individuals from distant locations. In contrast, Cx. pipiens f. molestus from New York City each contained a single SH60 variant, which was not identified in any other Cx. p. pipiens specimens analyzed. Supporting microsatellite analysis demonstrated significant but reduced gene flow between Cx. p. pipiens and Cx. pipiens f. molestus in New York relative to Cx. p. pipiens populations in New York and California. Results are discussed in the context of two contrasting hypotheses regarding the origin of Cx. pipiens f. molestus populations.</description><subject>Animals</subject><subject>Base Sequence</subject><subject>Culex</subject><subject>Culex pipiens</subject><subject>Culex pipiens form molestus</subject><subject>Culex pipiens pipiens</subject><subject>Culicidae</subject><subject>Diptera</subject><subject>gene flow</subject><subject>genetic markers</subject><subject>genetic variation</subject><subject>genetics</subject><subject>geographical distribution</subject><subject>Insect Vectors</subject><subject>microsatellite repeats</subject><subject>mitochondrial DNA</subject><subject>Molecular Sequence Data</subject><subject>New York</subject><subject>restriction fragment length polymorphism</subject><subject>ribosomal DNA</subject><subject>Sequence Alignment</subject><subject>single-stranded conformational polymorphism</subject><issn>0022-2585</issn><issn>0022-2585</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><recordid>eNqFUcFKw0AUDKJgrf7DnqQFG182u82mnmxiq1CsBz2IyJJs3sJqmsRsQvXvTaiKePE0j5nhvceM45x54HpT8M8BKJ1QLviIAgRjxp44zJbxPLpbPFMX3Gh9QfecwY9t_9d86BxZ-wIAwmPhwKmWWGBjFImN1lhjodCSOTZbxIJEbY7vpDKVwcIS7ZJNmaNtWkuSIvujfuMoNlWDdTLrdaNMluCYmILc4pY8lvXrsXOgk9ziyRcOnYfF1X10PVmtlzfR5WqiGATNJAEqUq4yTLTuGPDAzyhSDDPmC64QAt1RKAC5CL00RKG1n6U01SLkIsj8oXO621vV5VvbfS03xirM86TAsrWSBT6lU5_-a6TApl53szPGO6OqS2tr1LKqzSapP6QHsu9F9iHLPmTZ9yIZkxzkrhdJJchoLan_CaQyhGU</recordid><startdate>20070101</startdate><enddate>20070101</enddate><creator>Kent, Rebekah J.</creator><creator>Harrington, Laura C.</creator><creator>Norris, Douglas E.</creator><scope>AAYXX</scope><scope>CITATION</scope><scope>7SS</scope><scope>8FD</scope><scope>C1K</scope><scope>F1W</scope><scope>FR3</scope><scope>H95</scope><scope>H97</scope><scope>L.G</scope><scope>P64</scope><scope>RC3</scope><scope>7S9</scope><scope>L.6</scope></search><sort><creationdate>20070101</creationdate><title>Genetic Differences Between Culex pipiens f. molestus and Culex pipiens pipiens (Diptera: Culicidae) in New York</title><author>Kent, Rebekah J. ; Harrington, Laura C. ; Norris, Douglas E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c407t-a028b5cdeaffc400103d2e2e9d4385ce07f103e80e5891b9e8ff3db2bf89587d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Animals</topic><topic>Base Sequence</topic><topic>Culex</topic><topic>Culex pipiens</topic><topic>Culex pipiens form molestus</topic><topic>Culex pipiens pipiens</topic><topic>Culicidae</topic><topic>Diptera</topic><topic>gene flow</topic><topic>genetic markers</topic><topic>genetic variation</topic><topic>genetics</topic><topic>geographical distribution</topic><topic>Insect Vectors</topic><topic>microsatellite repeats</topic><topic>mitochondrial DNA</topic><topic>Molecular Sequence Data</topic><topic>New York</topic><topic>restriction fragment length polymorphism</topic><topic>ribosomal DNA</topic><topic>Sequence Alignment</topic><topic>single-stranded conformational polymorphism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kent, Rebekah J.</creatorcontrib><creatorcontrib>Harrington, Laura C.</creatorcontrib><creatorcontrib>Norris, Douglas E.</creatorcontrib><collection>CrossRef</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>Journal of medical entomology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kent, Rebekah J.</au><au>Harrington, Laura C.</au><au>Norris, Douglas E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genetic Differences Between Culex pipiens f. molestus and Culex pipiens pipiens (Diptera: Culicidae) in New York</atitle><jtitle>Journal of medical entomology</jtitle><date>2007-01-01</date><risdate>2007</risdate><volume>44</volume><issue>1</issue><spage>50</spage><epage>59</epage><pages>50-59</pages><issn>0022-2585</issn><eissn>0022-2585</eissn><abstract>The definition and phylogenetic placement of the autogenous molestus form of Culex pipiens has puzzled entomologists for decades. We identified genetic differences between Cx. p. pipiens (L.) and Cx. pipiens f. molestus Forskaal in the SH60 fragment described previously. Single-strand conformation polymorphism analysis, cloning, and sequencing of this fragment demonstrated high polymorphism within and among individual Cx. p. pipiens, with common SH60 variants shared among individuals from distant locations. In contrast, Cx. pipiens f. molestus from New York City each contained a single SH60 variant, which was not identified in any other Cx. p. pipiens specimens analyzed. Supporting microsatellite analysis demonstrated significant but reduced gene flow between Cx. p. pipiens and Cx. pipiens f. molestus in New York relative to Cx. p. pipiens populations in New York and California. Results are discussed in the context of two contrasting hypotheses regarding the origin of Cx. pipiens f. molestus populations.</abstract><doi>10.1603/0022-2585(2007)44[50:GDBCPF]2.0.CO;2</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0022-2585 |
| ispartof | Journal of medical entomology, 2007-01, Vol.44 (1), p.50-59 |
| issn | 0022-2585 0022-2585 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_47322632 |
| source | Oxford University Press Journals All Titles (1996-Current); BioOne Complete |
| subjects | Animals Base Sequence Culex Culex pipiens Culex pipiens form molestus Culex pipiens pipiens Culicidae Diptera gene flow genetic markers genetic variation genetics geographical distribution Insect Vectors microsatellite repeats mitochondrial DNA Molecular Sequence Data New York restriction fragment length polymorphism ribosomal DNA Sequence Alignment single-stranded conformational polymorphism |
| title | Genetic Differences Between Culex pipiens f. molestus and Culex pipiens pipiens (Diptera: Culicidae) in New York |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-07T23%3A11%3A17IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Genetic%20Differences%20Between%20Culex%20pipiens%20f.%20molestus%20and%20Culex%20pipiens%20pipiens%20(Diptera:%20Culicidae)%20in%20New%20York&rft.jtitle=Journal%20of%20medical%20entomology&rft.au=Kent,%20Rebekah%20J.&rft.date=2007-01-01&rft.volume=44&rft.issue=1&rft.spage=50&rft.epage=59&rft.pages=50-59&rft.issn=0022-2585&rft.eissn=0022-2585&rft_id=info:doi/10.1603/0022-2585(2007)44%5B50:GDBCPF%5D2.0.CO;2&rft_dat=%3Cproquest_cross%3E47322632%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=20461385&rft_id=info:pmid/&rfr_iscdi=true |