Microbial production of 2,3-butanediol by a mutagenized strain of Serratia marcescens H30
The production of 2,3-butanediol (2,3-BD) by Serratia marcescens H30 from sucrose was studied. Medium composition for 2,3-BD production by S. marcescens H30 was optimized in shake flask fermentations using Plackett–Burman design (PB) and response surface methodology (RSM). Results indicated that yea...
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| Veröffentlicht in: | Bioresource technology 2010-03, Vol.101 (6), p.1961-1967 |
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| container_end_page | 1967 |
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| container_issue | 6 |
| container_start_page | 1961 |
| container_title | Bioresource technology |
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| creator | Zhang, Liaoyuan Yang, Yunlong Sun, Jian’an Shen, Yaling Wei, Dongzhi Zhu, Jiawen Chu, Ju |
| description | The production of 2,3-butanediol (2,3-BD) by
Serratia marcescens H30 from sucrose was studied. Medium composition for 2,3-BD production by
S. marcescens H30 was optimized in shake flask fermentations using Plackett–Burman design (PB) and response surface methodology (RSM). Results indicated that yeast extract and sodium acetate had significant effects on the 2,3-BD production. And their optimal concentrations were determined by RSM. The optimal medium was used to perform fermentation experiments by
S. marcescens H30 in a 3.7
l bioreactor. Several feeding strategies including interim feeding, exponential feeding and constant residual sucrose concentration feeding were compared for improving 2,3-BD production. Ultimately, a suitable control strategy which combined the respiratory quotient (RQ) control with the constant residual sucrose concentration fed-batch was developed. Using this strategy, the maximum 2,3-BD concentration of 139.92
g/l with the diol (AC
+
BD) productivity of 3.49
g/l
h and the yield of 94.67% was obtained. |
| doi_str_mv | 10.1016/j.biortech.2009.10.052 |
| format | Article |
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Serratia marcescens H30 from sucrose was studied. Medium composition for 2,3-BD production by
S. marcescens H30 was optimized in shake flask fermentations using Plackett–Burman design (PB) and response surface methodology (RSM). Results indicated that yeast extract and sodium acetate had significant effects on the 2,3-BD production. And their optimal concentrations were determined by RSM. The optimal medium was used to perform fermentation experiments by
S. marcescens H30 in a 3.7
l bioreactor. Several feeding strategies including interim feeding, exponential feeding and constant residual sucrose concentration feeding were compared for improving 2,3-BD production. Ultimately, a suitable control strategy which combined the respiratory quotient (RQ) control with the constant residual sucrose concentration fed-batch was developed. Using this strategy, the maximum 2,3-BD concentration of 139.92
g/l with the diol (AC
+
BD) productivity of 3.49
g/l
h and the yield of 94.67% was obtained.</description><identifier>ISSN: 0960-8524</identifier><identifier>EISSN: 1873-2976</identifier><identifier>DOI: 10.1016/j.biortech.2009.10.052</identifier><identifier>PMID: 19932023</identifier><language>eng</language><publisher>Kidlington: Elsevier Ltd</publisher><subject>2,3-Butanediol ; Biological and medical sciences ; Butylene Glycols - chemistry ; Butylene Glycols - metabolism ; Chromatography, Gel ; Chromatography, Ion Exchange - methods ; Electrophoresis, Polyacrylamide Gel ; Fed-batch ; Fibrinogen - chemistry ; Fundamental and applied biological sciences. Psychology ; Hydrogen-Ion Concentration ; Isoelectric Focusing ; Medium optimization ; Molecular Weight ; Mutagenesis ; Peptide Hydrolases - chemistry ; Respiratory quotient ; Serine Proteases - chemistry ; Serratia marcescens ; Serratia marcescens - metabolism ; Serratia marcescens H30 ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ; Temperature ; Thrombosis - metabolism</subject><ispartof>Bioresource technology, 2010-03, Vol.101 (6), p.1961-1967</ispartof><rights>2009 Elsevier Ltd</rights><rights>2015 INIST-CNRS</rights><rights>Copyright (c) 2009 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c580t-86ec93162f885c5db5e8d4bd4c7f3e1f847bee3d32cf7f0b933d32c3a42f37683</citedby><cites>FETCH-LOGICAL-c580t-86ec93162f885c5db5e8d4bd4c7f3e1f847bee3d32cf7f0b933d32c3a42f37683</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.biortech.2009.10.052$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=22560604$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19932023$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhang, Liaoyuan</creatorcontrib><creatorcontrib>Yang, Yunlong</creatorcontrib><creatorcontrib>Sun, Jian’an</creatorcontrib><creatorcontrib>Shen, Yaling</creatorcontrib><creatorcontrib>Wei, Dongzhi</creatorcontrib><creatorcontrib>Zhu, Jiawen</creatorcontrib><creatorcontrib>Chu, Ju</creatorcontrib><title>Microbial production of 2,3-butanediol by a mutagenized strain of Serratia marcescens H30</title><title>Bioresource technology</title><addtitle>Bioresour Technol</addtitle><description>The production of 2,3-butanediol (2,3-BD) by
Serratia marcescens H30 from sucrose was studied. Medium composition for 2,3-BD production by
S. marcescens H30 was optimized in shake flask fermentations using Plackett–Burman design (PB) and response surface methodology (RSM). Results indicated that yeast extract and sodium acetate had significant effects on the 2,3-BD production. And their optimal concentrations were determined by RSM. The optimal medium was used to perform fermentation experiments by
S. marcescens H30 in a 3.7
l bioreactor. Several feeding strategies including interim feeding, exponential feeding and constant residual sucrose concentration feeding were compared for improving 2,3-BD production. Ultimately, a suitable control strategy which combined the respiratory quotient (RQ) control with the constant residual sucrose concentration fed-batch was developed. Using this strategy, the maximum 2,3-BD concentration of 139.92
g/l with the diol (AC
+
BD) productivity of 3.49
g/l
h and the yield of 94.67% was obtained.</description><subject>2,3-Butanediol</subject><subject>Biological and medical sciences</subject><subject>Butylene Glycols - chemistry</subject><subject>Butylene Glycols - metabolism</subject><subject>Chromatography, Gel</subject><subject>Chromatography, Ion Exchange - methods</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Fed-batch</subject><subject>Fibrinogen - chemistry</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hydrogen-Ion Concentration</subject><subject>Isoelectric Focusing</subject><subject>Medium optimization</subject><subject>Molecular Weight</subject><subject>Mutagenesis</subject><subject>Peptide Hydrolases - chemistry</subject><subject>Respiratory quotient</subject><subject>Serine Proteases - chemistry</subject><subject>Serratia marcescens</subject><subject>Serratia marcescens - metabolism</subject><subject>Serratia marcescens H30</subject><subject>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</subject><subject>Temperature</subject><subject>Thrombosis - metabolism</subject><issn>0960-8524</issn><issn>1873-2976</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU1v1DAQhi0EokvhL5Rc4ESWsSdxnBtVRSlSEYfSAyfLccbFq2xS7ASp_PrOdhc4lpO_Ho9fzyPEiYS1BKnfb9ZdnNJM_sdaAbS8uYZaPREraRosVdvop2IFrYbS1Ko6Ei9y3gAAykY9F0eybVGBwpX4_iX6NHXRDcVtmvrFz3EaiykU6h2W3TK7kfo4DUV3V7hiy-sbGuNv6os8JxcfyCtKyc2Rj13ylD2NubhAeCmeBTdkenUYj8X1-cdvZxfl5ddPn89OL0tfG5hLo8m3KLUKxtS-7ruaTF91feWbgCSDqZqOCHtUPjQBuhYf5ugqFbDRBo_F231dzv9zoTzbbeQQw8DRpyVb1Ij8b3gUVFIZ7lv1XyBW0DCo9yC3MOdEwd6myF24sxLsTpPd2D-a7E7Tbp818cWTwwtLt6X-37WDFwbeHACXvRtCcqOP-S-nVK1Bwy7q6z0X3GTdTWLm-kqBRGDR_OtdpQ97gtjBr0jJZh9p9Kw1kZ9tP8XH0t4DA5-8QQ</recordid><startdate>20100301</startdate><enddate>20100301</enddate><creator>Zhang, Liaoyuan</creator><creator>Yang, Yunlong</creator><creator>Sun, Jian’an</creator><creator>Shen, Yaling</creator><creator>Wei, Dongzhi</creator><creator>Zhu, Jiawen</creator><creator>Chu, Ju</creator><general>Elsevier Ltd</general><general>[New York, NY]: Elsevier Ltd</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7ST</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>SOI</scope><scope>7TB</scope><scope>KR7</scope></search><sort><creationdate>20100301</creationdate><title>Microbial production of 2,3-butanediol by a mutagenized strain of Serratia marcescens H30</title><author>Zhang, Liaoyuan ; Yang, Yunlong ; Sun, Jian’an ; Shen, Yaling ; Wei, Dongzhi ; Zhu, Jiawen ; Chu, Ju</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c580t-86ec93162f885c5db5e8d4bd4c7f3e1f847bee3d32cf7f0b933d32c3a42f37683</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>2,3-Butanediol</topic><topic>Biological and medical sciences</topic><topic>Butylene Glycols - chemistry</topic><topic>Butylene Glycols - metabolism</topic><topic>Chromatography, Gel</topic><topic>Chromatography, Ion Exchange - methods</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Fed-batch</topic><topic>Fibrinogen - chemistry</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hydrogen-Ion Concentration</topic><topic>Isoelectric Focusing</topic><topic>Medium optimization</topic><topic>Molecular Weight</topic><topic>Mutagenesis</topic><topic>Peptide Hydrolases - chemistry</topic><topic>Respiratory quotient</topic><topic>Serine Proteases - chemistry</topic><topic>Serratia marcescens</topic><topic>Serratia marcescens - metabolism</topic><topic>Serratia marcescens H30</topic><topic>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</topic><topic>Temperature</topic><topic>Thrombosis - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Liaoyuan</creatorcontrib><creatorcontrib>Yang, Yunlong</creatorcontrib><creatorcontrib>Sun, Jian’an</creatorcontrib><creatorcontrib>Shen, Yaling</creatorcontrib><creatorcontrib>Wei, Dongzhi</creatorcontrib><creatorcontrib>Zhu, Jiawen</creatorcontrib><creatorcontrib>Chu, Ju</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environment Abstracts</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Civil Engineering Abstracts</collection><jtitle>Bioresource technology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Liaoyuan</au><au>Yang, Yunlong</au><au>Sun, Jian’an</au><au>Shen, Yaling</au><au>Wei, Dongzhi</au><au>Zhu, Jiawen</au><au>Chu, Ju</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Microbial production of 2,3-butanediol by a mutagenized strain of Serratia marcescens H30</atitle><jtitle>Bioresource technology</jtitle><addtitle>Bioresour Technol</addtitle><date>2010-03-01</date><risdate>2010</risdate><volume>101</volume><issue>6</issue><spage>1961</spage><epage>1967</epage><pages>1961-1967</pages><issn>0960-8524</issn><eissn>1873-2976</eissn><abstract>The production of 2,3-butanediol (2,3-BD) by
Serratia marcescens H30 from sucrose was studied. Medium composition for 2,3-BD production by
S. marcescens H30 was optimized in shake flask fermentations using Plackett–Burman design (PB) and response surface methodology (RSM). Results indicated that yeast extract and sodium acetate had significant effects on the 2,3-BD production. And their optimal concentrations were determined by RSM. The optimal medium was used to perform fermentation experiments by
S. marcescens H30 in a 3.7
l bioreactor. Several feeding strategies including interim feeding, exponential feeding and constant residual sucrose concentration feeding were compared for improving 2,3-BD production. Ultimately, a suitable control strategy which combined the respiratory quotient (RQ) control with the constant residual sucrose concentration fed-batch was developed. Using this strategy, the maximum 2,3-BD concentration of 139.92
g/l with the diol (AC
+
BD) productivity of 3.49
g/l
h and the yield of 94.67% was obtained.</abstract><cop>Kidlington</cop><pub>Elsevier Ltd</pub><pmid>19932023</pmid><doi>10.1016/j.biortech.2009.10.052</doi><tpages>7</tpages></addata></record> |
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| ispartof | Bioresource technology, 2010-03, Vol.101 (6), p.1961-1967 |
| issn | 0960-8524 1873-2976 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | 2,3-Butanediol Biological and medical sciences Butylene Glycols - chemistry Butylene Glycols - metabolism Chromatography, Gel Chromatography, Ion Exchange - methods Electrophoresis, Polyacrylamide Gel Fed-batch Fibrinogen - chemistry Fundamental and applied biological sciences. Psychology Hydrogen-Ion Concentration Isoelectric Focusing Medium optimization Molecular Weight Mutagenesis Peptide Hydrolases - chemistry Respiratory quotient Serine Proteases - chemistry Serratia marcescens Serratia marcescens - metabolism Serratia marcescens H30 Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Temperature Thrombosis - metabolism |
| title | Microbial production of 2,3-butanediol by a mutagenized strain of Serratia marcescens H30 |
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