Transposon tagging and molecular analysis of the maize regulatory locus opaque-2
Genetic analyses suggested that the opaque-2 (o2) locus in maize acts as a positive, transacting, transcriptional activator of the zein seed storage-protein genes. Because isolation of the gene is requisite to understanding the molecular details of this regulation, transposon mutagenesis with the tr...
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| Veröffentlicht in: | Science (American Association for the Advancement of Science) 1987-11, Vol.238 (4829), p.960-963 |
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| creator | Schmidt, Robert J. Burr, Frances A. Burr, Benjamin |
| description | Genetic analyses suggested that the opaque-2 (o2) locus in maize acts as a positive, transacting, transcriptional activator of the zein seed storage-protein genes. Because isolation of the gene is requisite to understanding the molecular details of this regulation, transposon mutagenesis with the transposable element suppressor-mutator (Spm) was carried out, and three mutable o2 alleles were obtained. One of these alleles contained an 8.3-kilobase autonomous Spm, another a 6.8-kilobase nonautonomous Spm, and the third an unidentified transposon that is unrelated to Spm. A DNA sequence flanking the autonomous Spm insertion was verified to be o2-specific and provided a probe to clone a wild-type allele. Northern blots indicated that the gene is expressed in wild-type endosperm but not in leaf tissues or in endosperms homozygous for a mutant allele of the O2 gene. A transcript was detected in endosperms homozygous for mutations at opaque-7 and floury-2, an indication that O2 expression is independent of these two other putative regulators of zein synthesis. |
| doi_str_mv | 10.1126/science.2823388 |
| format | Article |
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Because isolation of the gene is requisite to understanding the molecular details of this regulation, transposon mutagenesis with the transposable element suppressor-mutator (Spm) was carried out, and three mutable o2 alleles were obtained. One of these alleles contained an 8.3-kilobase autonomous Spm, another a 6.8-kilobase nonautonomous Spm, and the third an unidentified transposon that is unrelated to Spm. A DNA sequence flanking the autonomous Spm insertion was verified to be o2-specific and provided a probe to clone a wild-type allele. Northern blots indicated that the gene is expressed in wild-type endosperm but not in leaf tissues or in endosperms homozygous for a mutant allele of the O2 gene. A transcript was detected in endosperms homozygous for mutations at opaque-7 and floury-2, an indication that O2 expression is independent of these two other putative regulators of zein synthesis.</description><identifier>ISSN: 0036-8075</identifier><identifier>EISSN: 1095-9203</identifier><identifier>DOI: 10.1126/science.2823388</identifier><identifier>PMID: 2823388</identifier><identifier>CODEN: SCIEAS</identifier><language>eng</language><publisher>Washington, DC: The American Association for the Advancement of Science</publisher><subject>550200 - Biochemistry ; adn ; Agronomy. Soil science and plant productions ; Alleles ; BASIC BIOLOGICAL SCIENCES ; Biological and medical sciences ; CEREALS ; Classical and quantitative genetics. Population genetics. Molecular genetics ; Cloning, Molecular ; Corn ; DNA ; DNA probes ; DNA Restriction Enzymes ; DNA SEQUENCING ; DNA Transposable Elements ; Endosperm ; Enzymes ; Flowers & plants ; Fundamental and applied biological sciences. Psychology ; gene ; GENE REGULATION ; Generalities. Genetics. Plant material ; GENES ; Genes, Regulator ; Genes. Genome ; Genetic aspects ; Genetic loci ; genetica ; Genetics ; Genetics and breeding of economic plants ; genetique ; GRASS ; Homozygote ; MAIZE ; Molecular and cellular biology ; MOLECULAR BIOLOGY ; Molecular genetics ; MUTAGENESIS ; Mutation ; Nucleic Acid Hybridization ; Phenotypes ; Plant genetic engineering ; PLANTS ; Plants - genetics ; STRUCTURAL CHEMICAL ANALYSIS ; TRANSCRIPTION ; Translocation (Genetics) ; Translocations (Genetics) ; Transposons ; Zea mays ; Zea mays - genetics</subject><ispartof>Science (American Association for the Advancement of Science), 1987-11, Vol.238 (4829), p.960-963</ispartof><rights>Copyright 1987 The American Association for the Advancement of Science</rights><rights>1988 INIST-CNRS</rights><rights>COPYRIGHT 1987 American Association for the Advancement of Science</rights><rights>Copyright American Association for the Advancement of Science Nov 13, 1987</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c774t-84d32c65f747fb57d082273bfcf237d6bd764dd5e2fca4733228ca96ed83fbec3</citedby><cites>FETCH-LOGICAL-c774t-84d32c65f747fb57d082273bfcf237d6bd764dd5e2fca4733228ca96ed83fbec3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/1700935$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/1700935$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,780,784,803,885,2884,2885,27924,27925,58017,58250</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7475706$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2823388$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/7242431$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Schmidt, Robert J.</creatorcontrib><creatorcontrib>Burr, Frances A.</creatorcontrib><creatorcontrib>Burr, Benjamin</creatorcontrib><creatorcontrib>Brookhaven National Lab., Upton, NY (USA)</creatorcontrib><title>Transposon tagging and molecular analysis of the maize regulatory locus opaque-2</title><title>Science (American Association for the Advancement of Science)</title><addtitle>Science</addtitle><description>Genetic analyses suggested that the opaque-2 (o2) locus in maize acts as a positive, transacting, transcriptional activator of the zein seed storage-protein genes. Because isolation of the gene is requisite to understanding the molecular details of this regulation, transposon mutagenesis with the transposable element suppressor-mutator (Spm) was carried out, and three mutable o2 alleles were obtained. One of these alleles contained an 8.3-kilobase autonomous Spm, another a 6.8-kilobase nonautonomous Spm, and the third an unidentified transposon that is unrelated to Spm. A DNA sequence flanking the autonomous Spm insertion was verified to be o2-specific and provided a probe to clone a wild-type allele. Northern blots indicated that the gene is expressed in wild-type endosperm but not in leaf tissues or in endosperms homozygous for a mutant allele of the O2 gene. A transcript was detected in endosperms homozygous for mutations at opaque-7 and floury-2, an indication that O2 expression is independent of these two other putative regulators of zein synthesis.</description><subject>550200 - Biochemistry</subject><subject>adn</subject><subject>Agronomy. Soil science and plant productions</subject><subject>Alleles</subject><subject>BASIC BIOLOGICAL SCIENCES</subject><subject>Biological and medical sciences</subject><subject>CEREALS</subject><subject>Classical and quantitative genetics. Population genetics. Molecular genetics</subject><subject>Cloning, Molecular</subject><subject>Corn</subject><subject>DNA</subject><subject>DNA probes</subject><subject>DNA Restriction Enzymes</subject><subject>DNA SEQUENCING</subject><subject>DNA Transposable Elements</subject><subject>Endosperm</subject><subject>Enzymes</subject><subject>Flowers & plants</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>gene</subject><subject>GENE REGULATION</subject><subject>Generalities. Genetics. Plant material</subject><subject>GENES</subject><subject>Genes, Regulator</subject><subject>Genes. Genome</subject><subject>Genetic aspects</subject><subject>Genetic loci</subject><subject>genetica</subject><subject>Genetics</subject><subject>Genetics and breeding of economic plants</subject><subject>genetique</subject><subject>GRASS</subject><subject>Homozygote</subject><subject>MAIZE</subject><subject>Molecular and cellular biology</subject><subject>MOLECULAR BIOLOGY</subject><subject>Molecular genetics</subject><subject>MUTAGENESIS</subject><subject>Mutation</subject><subject>Nucleic Acid Hybridization</subject><subject>Phenotypes</subject><subject>Plant genetic engineering</subject><subject>PLANTS</subject><subject>Plants - genetics</subject><subject>STRUCTURAL CHEMICAL ANALYSIS</subject><subject>TRANSCRIPTION</subject><subject>Translocation (Genetics)</subject><subject>Translocations (Genetics)</subject><subject>Transposons</subject><subject>Zea mays</subject><subject>Zea mays - genetics</subject><issn>0036-8075</issn><issn>1095-9203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqN00Fv0zAUB_AIgUYZnLkAChOCA2RzbCd2jqOCMqmiSNu4Wq7znKVy4mInEt2nx1WidkhFVDlEyf-X58R5L4pepug8TXF-4VUNrYJzzDEhnD-KJikqsqTAiDyOJgiRPOGIZU-jZ96vEApZQU6ik5FPoh83TrZ-bb1t405WVd1WsWzLuLEGVG-kC1fSbHztY6vj7g7iRtb3EDuoQtpZt4mNVX1I1_JXDwl-Hj3R0nh4MZ5Po9uvX26m35L5YnY1vZwnijHaJZyWBKs804wyvcxYiTjGjCy10piwMl-WLKdlmQHWSlJGCMZcySKHkhO9BEVOo7OhrvVdLcI2dKDulG1bUJ1gmGJK0oDeD2jtbHg734mm9gqMkS3Y3guSE0RRlv0XprTAKUFkv-wOrmzvwhZ5EUCGC0JpQG8HVEkDom617ZxU24riMk-z8CnbQh8PmApacNLYFnQdbj_Qnw7ocJTQ1OoA__AXD6KD310le-_F1fX3Y-Xi57Hy8-xIyWfzf2_CKJU1BioQoWGmi4f6YtDKWe8daLF2dSPdRqRIbKdBjNMgxvYOT7we_1a_bKDc-X3-bsylV9LoMAuq9jsWejNjKA_s1cBWPnT8flUW5olsm-fNEGtphaxcqHB7nRacI5SnOafkDyUlH8I</recordid><startdate>19871113</startdate><enddate>19871113</enddate><creator>Schmidt, Robert J.</creator><creator>Burr, Frances A.</creator><creator>Burr, Benjamin</creator><general>The American Association for the Advancement of Science</general><general>American Association for the Advancement of Science</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8GL</scope><scope>IBG</scope><scope>IOV</scope><scope>ISN</scope><scope>7QF</scope><scope>7QG</scope><scope>7QL</scope><scope>7QP</scope><scope>7QQ</scope><scope>7QR</scope><scope>7SC</scope><scope>7SE</scope><scope>7SN</scope><scope>7SP</scope><scope>7SR</scope><scope>7SS</scope><scope>7T7</scope><scope>7TA</scope><scope>7TB</scope><scope>7TK</scope><scope>7TM</scope><scope>7U5</scope><scope>7U9</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>H94</scope><scope>JG9</scope><scope>JQ2</scope><scope>K9.</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>OTOTI</scope></search><sort><creationdate>19871113</creationdate><title>Transposon tagging and molecular analysis of the maize regulatory locus opaque-2</title><author>Schmidt, Robert J. ; Burr, Frances A. ; Burr, Benjamin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c774t-84d32c65f747fb57d082273bfcf237d6bd764dd5e2fca4733228ca96ed83fbec3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1987</creationdate><topic>550200 - Biochemistry</topic><topic>adn</topic><topic>Agronomy. Soil science and plant productions</topic><topic>Alleles</topic><topic>BASIC BIOLOGICAL SCIENCES</topic><topic>Biological and medical sciences</topic><topic>CEREALS</topic><topic>Classical and quantitative genetics. Population genetics. Molecular genetics</topic><topic>Cloning, Molecular</topic><topic>Corn</topic><topic>DNA</topic><topic>DNA probes</topic><topic>DNA Restriction Enzymes</topic><topic>DNA SEQUENCING</topic><topic>DNA Transposable Elements</topic><topic>Endosperm</topic><topic>Enzymes</topic><topic>Flowers & plants</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>gene</topic><topic>GENE REGULATION</topic><topic>Generalities. Genetics. Plant material</topic><topic>GENES</topic><topic>Genes, Regulator</topic><topic>Genes. Genome</topic><topic>Genetic aspects</topic><topic>Genetic loci</topic><topic>genetica</topic><topic>Genetics</topic><topic>Genetics and breeding of economic plants</topic><topic>genetique</topic><topic>GRASS</topic><topic>Homozygote</topic><topic>MAIZE</topic><topic>Molecular and cellular biology</topic><topic>MOLECULAR BIOLOGY</topic><topic>Molecular genetics</topic><topic>MUTAGENESIS</topic><topic>Mutation</topic><topic>Nucleic Acid Hybridization</topic><topic>Phenotypes</topic><topic>Plant genetic engineering</topic><topic>PLANTS</topic><topic>Plants - genetics</topic><topic>STRUCTURAL CHEMICAL ANALYSIS</topic><topic>TRANSCRIPTION</topic><topic>Translocation (Genetics)</topic><topic>Translocations (Genetics)</topic><topic>Transposons</topic><topic>Zea mays</topic><topic>Zea mays - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Schmidt, Robert J.</creatorcontrib><creatorcontrib>Burr, Frances A.</creatorcontrib><creatorcontrib>Burr, Benjamin</creatorcontrib><creatorcontrib>Brookhaven National Lab., Upton, NY (USA)</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: High School</collection><collection>Gale In Context: Biography</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Canada</collection><collection>Aluminium Industry Abstracts</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Ecology Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>OSTI.GOV</collection><jtitle>Science (American Association for the Advancement of Science)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Schmidt, Robert J.</au><au>Burr, Frances A.</au><au>Burr, Benjamin</au><aucorp>Brookhaven National Lab., Upton, NY (USA)</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Transposon tagging and molecular analysis of the maize regulatory locus opaque-2</atitle><jtitle>Science (American Association for the Advancement of Science)</jtitle><addtitle>Science</addtitle><date>1987-11-13</date><risdate>1987</risdate><volume>238</volume><issue>4829</issue><spage>960</spage><epage>963</epage><pages>960-963</pages><issn>0036-8075</issn><eissn>1095-9203</eissn><coden>SCIEAS</coden><abstract>Genetic analyses suggested that the opaque-2 (o2) locus in maize acts as a positive, transacting, transcriptional activator of the zein seed storage-protein genes. Because isolation of the gene is requisite to understanding the molecular details of this regulation, transposon mutagenesis with the transposable element suppressor-mutator (Spm) was carried out, and three mutable o2 alleles were obtained. One of these alleles contained an 8.3-kilobase autonomous Spm, another a 6.8-kilobase nonautonomous Spm, and the third an unidentified transposon that is unrelated to Spm. A DNA sequence flanking the autonomous Spm insertion was verified to be o2-specific and provided a probe to clone a wild-type allele. Northern blots indicated that the gene is expressed in wild-type endosperm but not in leaf tissues or in endosperms homozygous for a mutant allele of the O2 gene. A transcript was detected in endosperms homozygous for mutations at opaque-7 and floury-2, an indication that O2 expression is independent of these two other putative regulators of zein synthesis.</abstract><cop>Washington, DC</cop><pub>The American Association for the Advancement of Science</pub><pmid>2823388</pmid><doi>10.1126/science.2823388</doi><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0036-8075 |
| ispartof | Science (American Association for the Advancement of Science), 1987-11, Vol.238 (4829), p.960-963 |
| issn | 0036-8075 1095-9203 |
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| source | MEDLINE; JSTOR Archive Collection A-Z Listing; American Association for the Advancement of Science |
| subjects | 550200 - Biochemistry adn Agronomy. Soil science and plant productions Alleles BASIC BIOLOGICAL SCIENCES Biological and medical sciences CEREALS Classical and quantitative genetics. Population genetics. Molecular genetics Cloning, Molecular Corn DNA DNA probes DNA Restriction Enzymes DNA SEQUENCING DNA Transposable Elements Endosperm Enzymes Flowers & plants Fundamental and applied biological sciences. Psychology gene GENE REGULATION Generalities. Genetics. Plant material GENES Genes, Regulator Genes. Genome Genetic aspects Genetic loci genetica Genetics Genetics and breeding of economic plants genetique GRASS Homozygote MAIZE Molecular and cellular biology MOLECULAR BIOLOGY Molecular genetics MUTAGENESIS Mutation Nucleic Acid Hybridization Phenotypes Plant genetic engineering PLANTS Plants - genetics STRUCTURAL CHEMICAL ANALYSIS TRANSCRIPTION Translocation (Genetics) Translocations (Genetics) Transposons Zea mays Zea mays - genetics |
| title | Transposon tagging and molecular analysis of the maize regulatory locus opaque-2 |
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