Scaffolds with covalently immobilized VEGF and Angiopoietin-1 for vascularization of engineered tissues
Abstract The aim of this study was to engineer a biomaterial capable of supporting vascularization in vitro and in vivo . We covalently immobilized vascular endothelial growth factor (VEGF) and Angiopoietin-1 (Ang1) onto three-dimensional porous collagen scaffolds using 1-ethyl-3-[3-dimethylaminopro...
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| Veröffentlicht in: | Biomaterials 2010-01, Vol.31 (2), p.226-241 |
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| creator | Chiu, Loraine L.Y Radisic, Milica |
| description | Abstract The aim of this study was to engineer a biomaterial capable of supporting vascularization in vitro and in vivo . We covalently immobilized vascular endothelial growth factor (VEGF) and Angiopoietin-1 (Ang1) onto three-dimensional porous collagen scaffolds using 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride (EDC) chemistry. Over both 3 and 7 days in vitro , seeded endothelial cells (ECs) had increased proliferation on scaffolds with immobilized VEGF and/or Ang1 compared to unmodified scaffolds and soluble growth factor controls. Notably, the group with co-immobilized VEGF and Ang1 showed significantly higher cell number ( P = 0.0079), higher overall lactate production rate ( P = 0.0044) and higher overall glucose consumption rate ( P = 0.0034) at Day 3, compared to its corresponding soluble control for which growth factors were added to culture medium. By Day 7, hematoxylin and eosin, live/dead, CD31, and von Willebrand factor staining all showed improved tube formation by ECs when cultivated on scaffolds with co-immobilized growth factors. Interestingly, scaffolds with co-immobilized VEGF and Ang1 showed increased EC infiltration in the chorioallantoic membrane (CAM) assay, compared to scaffolds with independently immobilized VEGF/Ang1. This study presents an alternative method for promoting the formation of vascular structures, via covalent immobilization of angiogenic growth factors that are more stable than soluble ones and have a localized effect. |
| doi_str_mv | 10.1016/j.biomaterials.2009.09.039 |
| format | Article |
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We covalently immobilized vascular endothelial growth factor (VEGF) and Angiopoietin-1 (Ang1) onto three-dimensional porous collagen scaffolds using 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride (EDC) chemistry. Over both 3 and 7 days in vitro , seeded endothelial cells (ECs) had increased proliferation on scaffolds with immobilized VEGF and/or Ang1 compared to unmodified scaffolds and soluble growth factor controls. Notably, the group with co-immobilized VEGF and Ang1 showed significantly higher cell number ( P = 0.0079), higher overall lactate production rate ( P = 0.0044) and higher overall glucose consumption rate ( P = 0.0034) at Day 3, compared to its corresponding soluble control for which growth factors were added to culture medium. By Day 7, hematoxylin and eosin, live/dead, CD31, and von Willebrand factor staining all showed improved tube formation by ECs when cultivated on scaffolds with co-immobilized growth factors. Interestingly, scaffolds with co-immobilized VEGF and Ang1 showed increased EC infiltration in the chorioallantoic membrane (CAM) assay, compared to scaffolds with independently immobilized VEGF/Ang1. This study presents an alternative method for promoting the formation of vascular structures, via covalent immobilization of angiogenic growth factors that are more stable than soluble ones and have a localized effect.</description><identifier>ISSN: 0142-9612</identifier><identifier>EISSN: 1878-5905</identifier><identifier>DOI: 10.1016/j.biomaterials.2009.09.039</identifier><identifier>PMID: 19800684</identifier><language>eng</language><publisher>Netherlands: Elsevier Ltd</publisher><subject>Advanced Basic Science ; Angiogenesis ; Angiopoietin-1 - pharmacology ; Animals ; Biological Assay ; Cardiac tissue engineering ; Cell Line ; Cell Proliferation - drug effects ; Cell Shape - drug effects ; Chickens ; Chorioallantoic Membrane - drug effects ; Chorioallantoic Membrane - metabolism ; Collagen ; Collagen - pharmacology ; Collagenases - metabolism ; Dentistry ; Endothelial cell ; Endothelial Cells - cytology ; Endothelial Cells - drug effects ; Endothelial Cells - metabolism ; Enzyme-Linked Immunosorbent Assay ; Growth factors ; Hemoglobins - metabolism ; Humans ; Hydrolysis - drug effects ; Immobilized Proteins - pharmacology ; Mice ; Microscopy, Electron, Scanning ; Neovascularization, Physiologic - drug effects ; Scaffold ; Solubility - drug effects ; Tissue Engineering ; Tissue Scaffolds - chemistry ; Vascular Endothelial Growth Factor A - pharmacology</subject><ispartof>Biomaterials, 2010-01, Vol.31 (2), p.226-241</ispartof><rights>Elsevier Ltd</rights><rights>2009 Elsevier Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c464t-9bdf3c982d7544fab23a4aa4cccea792868f6112ff639d085f5627a506f6e2583</citedby><cites>FETCH-LOGICAL-c464t-9bdf3c982d7544fab23a4aa4cccea792868f6112ff639d085f5627a506f6e2583</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.biomaterials.2009.09.039$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19800684$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chiu, Loraine L.Y</creatorcontrib><creatorcontrib>Radisic, Milica</creatorcontrib><title>Scaffolds with covalently immobilized VEGF and Angiopoietin-1 for vascularization of engineered tissues</title><title>Biomaterials</title><addtitle>Biomaterials</addtitle><description>Abstract The aim of this study was to engineer a biomaterial capable of supporting vascularization in vitro and in vivo . We covalently immobilized vascular endothelial growth factor (VEGF) and Angiopoietin-1 (Ang1) onto three-dimensional porous collagen scaffolds using 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride (EDC) chemistry. Over both 3 and 7 days in vitro , seeded endothelial cells (ECs) had increased proliferation on scaffolds with immobilized VEGF and/or Ang1 compared to unmodified scaffolds and soluble growth factor controls. Notably, the group with co-immobilized VEGF and Ang1 showed significantly higher cell number ( P = 0.0079), higher overall lactate production rate ( P = 0.0044) and higher overall glucose consumption rate ( P = 0.0034) at Day 3, compared to its corresponding soluble control for which growth factors were added to culture medium. By Day 7, hematoxylin and eosin, live/dead, CD31, and von Willebrand factor staining all showed improved tube formation by ECs when cultivated on scaffolds with co-immobilized growth factors. Interestingly, scaffolds with co-immobilized VEGF and Ang1 showed increased EC infiltration in the chorioallantoic membrane (CAM) assay, compared to scaffolds with independently immobilized VEGF/Ang1. This study presents an alternative method for promoting the formation of vascular structures, via covalent immobilization of angiogenic growth factors that are more stable than soluble ones and have a localized effect.</description><subject>Advanced Basic Science</subject><subject>Angiogenesis</subject><subject>Angiopoietin-1 - pharmacology</subject><subject>Animals</subject><subject>Biological Assay</subject><subject>Cardiac tissue engineering</subject><subject>Cell Line</subject><subject>Cell Proliferation - drug effects</subject><subject>Cell Shape - drug effects</subject><subject>Chickens</subject><subject>Chorioallantoic Membrane - drug effects</subject><subject>Chorioallantoic Membrane - metabolism</subject><subject>Collagen</subject><subject>Collagen - pharmacology</subject><subject>Collagenases - metabolism</subject><subject>Dentistry</subject><subject>Endothelial cell</subject><subject>Endothelial Cells - cytology</subject><subject>Endothelial Cells - drug effects</subject><subject>Endothelial Cells - metabolism</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Growth factors</subject><subject>Hemoglobins - metabolism</subject><subject>Humans</subject><subject>Hydrolysis - drug effects</subject><subject>Immobilized Proteins - pharmacology</subject><subject>Mice</subject><subject>Microscopy, Electron, Scanning</subject><subject>Neovascularization, Physiologic - drug effects</subject><subject>Scaffold</subject><subject>Solubility - drug effects</subject><subject>Tissue Engineering</subject><subject>Tissue Scaffolds - chemistry</subject><subject>Vascular Endothelial Growth Factor A - pharmacology</subject><issn>0142-9612</issn><issn>1878-5905</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNklFrFDEUhYModq3-BQk--DZrkslkEh-EUtsqFHyo-hqymZuaNZOsyczK9tebYRcUXxQuhMB3zgk5F6FXlKwpoeLNdr3xaTQTZG9CWTNC1HqZVj1CKyp72XSKdI_RilDOGiUoO0PPStmSeiecPUVnVElChOQrdH9njXMpDAX_9NM3bNPeBIhTOGA_jmnjg3-AAX-9urnGJg74It77tEseJh8bil3KeG-KnYPJ_sFMPkWcHIZKRYBclZMvZYbyHD1x9bHw4nSeoy_XV58vPzS3n24-Xl7cNpYLPjVqM7jWKsmGvuPcmQ1rDTeGW2vB9IpJIZ2glDknWjUQ2blOsN50RDgBrJPtOXp99N3l9KPmTnr0xUIIJkKai24FI5L2_wYZJZJTwSr49gjanErJ4PQu-9Hkg6ZEL33orf6zD730oZdpVRW_PKXMmxGG39JTARV4fwSgfsreQ9bFeogWBp_BTnpI_v9y3v1lY4OP3prwHQ5QtmnOcdFQXZgm-m7ZjGUxiKomQvXtL4ObuXI</recordid><startdate>20100101</startdate><enddate>20100101</enddate><creator>Chiu, Loraine L.Y</creator><creator>Radisic, Milica</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7SR</scope><scope>7TB</scope><scope>7U5</scope><scope>8BQ</scope><scope>F28</scope><scope>JG9</scope><scope>L7M</scope></search><sort><creationdate>20100101</creationdate><title>Scaffolds with covalently immobilized VEGF and Angiopoietin-1 for vascularization of engineered tissues</title><author>Chiu, Loraine L.Y ; Radisic, Milica</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c464t-9bdf3c982d7544fab23a4aa4cccea792868f6112ff639d085f5627a506f6e2583</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Advanced Basic Science</topic><topic>Angiogenesis</topic><topic>Angiopoietin-1 - pharmacology</topic><topic>Animals</topic><topic>Biological Assay</topic><topic>Cardiac tissue engineering</topic><topic>Cell Line</topic><topic>Cell Proliferation - drug effects</topic><topic>Cell Shape - drug effects</topic><topic>Chickens</topic><topic>Chorioallantoic Membrane - drug effects</topic><topic>Chorioallantoic Membrane - metabolism</topic><topic>Collagen</topic><topic>Collagen - pharmacology</topic><topic>Collagenases - metabolism</topic><topic>Dentistry</topic><topic>Endothelial cell</topic><topic>Endothelial Cells - cytology</topic><topic>Endothelial Cells - drug effects</topic><topic>Endothelial Cells - metabolism</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Growth factors</topic><topic>Hemoglobins - metabolism</topic><topic>Humans</topic><topic>Hydrolysis - drug effects</topic><topic>Immobilized Proteins - pharmacology</topic><topic>Mice</topic><topic>Microscopy, Electron, Scanning</topic><topic>Neovascularization, Physiologic - drug effects</topic><topic>Scaffold</topic><topic>Solubility - drug effects</topic><topic>Tissue Engineering</topic><topic>Tissue Scaffolds - chemistry</topic><topic>Vascular Endothelial Growth Factor A - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chiu, Loraine L.Y</creatorcontrib><creatorcontrib>Radisic, Milica</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><jtitle>Biomaterials</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chiu, Loraine L.Y</au><au>Radisic, Milica</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Scaffolds with covalently immobilized VEGF and Angiopoietin-1 for vascularization of engineered tissues</atitle><jtitle>Biomaterials</jtitle><addtitle>Biomaterials</addtitle><date>2010-01-01</date><risdate>2010</risdate><volume>31</volume><issue>2</issue><spage>226</spage><epage>241</epage><pages>226-241</pages><issn>0142-9612</issn><eissn>1878-5905</eissn><abstract>Abstract The aim of this study was to engineer a biomaterial capable of supporting vascularization in vitro and in vivo . We covalently immobilized vascular endothelial growth factor (VEGF) and Angiopoietin-1 (Ang1) onto three-dimensional porous collagen scaffolds using 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride (EDC) chemistry. Over both 3 and 7 days in vitro , seeded endothelial cells (ECs) had increased proliferation on scaffolds with immobilized VEGF and/or Ang1 compared to unmodified scaffolds and soluble growth factor controls. Notably, the group with co-immobilized VEGF and Ang1 showed significantly higher cell number ( P = 0.0079), higher overall lactate production rate ( P = 0.0044) and higher overall glucose consumption rate ( P = 0.0034) at Day 3, compared to its corresponding soluble control for which growth factors were added to culture medium. By Day 7, hematoxylin and eosin, live/dead, CD31, and von Willebrand factor staining all showed improved tube formation by ECs when cultivated on scaffolds with co-immobilized growth factors. Interestingly, scaffolds with co-immobilized VEGF and Ang1 showed increased EC infiltration in the chorioallantoic membrane (CAM) assay, compared to scaffolds with independently immobilized VEGF/Ang1. This study presents an alternative method for promoting the formation of vascular structures, via covalent immobilization of angiogenic growth factors that are more stable than soluble ones and have a localized effect.</abstract><cop>Netherlands</cop><pub>Elsevier Ltd</pub><pmid>19800684</pmid><doi>10.1016/j.biomaterials.2009.09.039</doi><tpages>16</tpages></addata></record> |
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| subjects | Advanced Basic Science Angiogenesis Angiopoietin-1 - pharmacology Animals Biological Assay Cardiac tissue engineering Cell Line Cell Proliferation - drug effects Cell Shape - drug effects Chickens Chorioallantoic Membrane - drug effects Chorioallantoic Membrane - metabolism Collagen Collagen - pharmacology Collagenases - metabolism Dentistry Endothelial cell Endothelial Cells - cytology Endothelial Cells - drug effects Endothelial Cells - metabolism Enzyme-Linked Immunosorbent Assay Growth factors Hemoglobins - metabolism Humans Hydrolysis - drug effects Immobilized Proteins - pharmacology Mice Microscopy, Electron, Scanning Neovascularization, Physiologic - drug effects Scaffold Solubility - drug effects Tissue Engineering Tissue Scaffolds - chemistry Vascular Endothelial Growth Factor A - pharmacology |
| title | Scaffolds with covalently immobilized VEGF and Angiopoietin-1 for vascularization of engineered tissues |
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