Effect of gamma irradiation on cell lysis and polyhydroxyalkanoate produced by Bacillus flexus
Bacillus flexus cultivated on sucrose and sucrose with plant oil such as castor oil produced polyhydroxybutyrate (PHB), a homopolymer of polyhydroxyalkanoate (PHA) and PHA copolymer (containing hydroxybutyrate and hexanoate), respectively. Gamma irradiation of these cells (5–40 kGy) resulted in cell...
Gespeichert in:
| Veröffentlicht in: | Radiation physics and chemistry (Oxford, England : 1993) England : 1993), 2009-02, Vol.78 (2), p.147-152 |
|---|---|
| Hauptverfasser: | , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 152 |
|---|---|
| container_issue | 2 |
| container_start_page | 147 |
| container_title | Radiation physics and chemistry (Oxford, England : 1993) |
| container_volume | 78 |
| creator | Divyashree, M.S. Shamala, T.R. |
| description | Bacillus flexus cultivated on sucrose and sucrose with plant oil such as castor oil produced polyhydroxybutyrate (PHB), a homopolymer of polyhydroxyalkanoate (PHA) and PHA copolymer (containing hydroxybutyrate and hexanoate), respectively. Gamma irradiation of these cells (5–40
kGy) resulted in cell damage and aided in the isolation of 45% and 54% PHA on biomass weight, correspondingly. Molecular weight of PHB increased from 1.5×10
5 to 1.9×10
5 after irradiation (10
kGy), with marginal increase of tensile strength from 18 to 20
MPa. At the same irradiation dosage, PHA copolymer showed higher molecular weight increase from 1.7×10
5 to 2.3×10
5 and tensile strength from 20 to 35
MPa. GC, GC–MS, FTIR and
1H NMR were used for the characterization of PHA. Gamma irradiation seems to be a novel technique, to induce cross-linking and molecular weight increase of PHA copolymer and aid in easy extractability of intracellular PHA, simultaneously. |
| doi_str_mv | 10.1016/j.radphyschem.2008.08.010 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_35370819</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0969806X0800203X</els_id><sourcerecordid>35370819</sourcerecordid><originalsourceid>FETCH-LOGICAL-c352t-80b6a2db0fca7b17367179c4c2c25c55a84c1e8f2d9b1c728dc738bf8c841ab53</originalsourceid><addsrcrecordid>eNqNkD9PwzAUxC0EEqXwHczC1mInTeyMUPFPqsQCEhOW82xTFycOdoKab4-jMjAinfSWd6e7H0KXlCwpoeX1bhmk6rZjhK1ulhkhfDmJkiM0o5xVC8Kr4hjNSFVWC07Kt1N0FuOOEMJ4kc_Q-50xGnrsDf6QTSOxDSnQyt76FieBdg67MdqIZatw5924HVXw-1G6T9l62WvcBa8G0ArXI76VYJ0bIjZO74d4jk6MdFFf_N45er2_e1k_LjbPD0_rm80C8iLrU7G6lJmqiQHJasryklFWwQoyyAooCslXQDU3mapqCizjCljOa8OBr6isi3yOrg65qcvXoGMvGhun7rLVfogiL3JGOK3SY3V4hOBjDNqILthGhlFQIiaiYif-EBUTUTGJkuRdH7w6Lfm2OogIVrdpuQ2JoVDe_iPlB_awh_E</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>35370819</pqid></control><display><type>article</type><title>Effect of gamma irradiation on cell lysis and polyhydroxyalkanoate produced by Bacillus flexus</title><source>ScienceDirect Journals (5 years ago - present)</source><creator>Divyashree, M.S. ; Shamala, T.R.</creator><creatorcontrib>Divyashree, M.S. ; Shamala, T.R.</creatorcontrib><description>Bacillus flexus cultivated on sucrose and sucrose with plant oil such as castor oil produced polyhydroxybutyrate (PHB), a homopolymer of polyhydroxyalkanoate (PHA) and PHA copolymer (containing hydroxybutyrate and hexanoate), respectively. Gamma irradiation of these cells (5–40
kGy) resulted in cell damage and aided in the isolation of 45% and 54% PHA on biomass weight, correspondingly. Molecular weight of PHB increased from 1.5×10
5 to 1.9×10
5 after irradiation (10
kGy), with marginal increase of tensile strength from 18 to 20
MPa. At the same irradiation dosage, PHA copolymer showed higher molecular weight increase from 1.7×10
5 to 2.3×10
5 and tensile strength from 20 to 35
MPa. GC, GC–MS, FTIR and
1H NMR were used for the characterization of PHA. Gamma irradiation seems to be a novel technique, to induce cross-linking and molecular weight increase of PHA copolymer and aid in easy extractability of intracellular PHA, simultaneously.</description><identifier>ISSN: 0969-806X</identifier><identifier>EISSN: 1879-0895</identifier><identifier>DOI: 10.1016/j.radphyschem.2008.08.010</identifier><language>eng</language><publisher>Elsevier Ltd</publisher><subject>1H NMR spectrum ; Bacillus flexus ; FTIR spectrum ; Gamma irradiation ; Polyhydroxyalkanoate copolymer ; Polyhydroxybutyrate</subject><ispartof>Radiation physics and chemistry (Oxford, England : 1993), 2009-02, Vol.78 (2), p.147-152</ispartof><rights>2008 Elsevier Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c352t-80b6a2db0fca7b17367179c4c2c25c55a84c1e8f2d9b1c728dc738bf8c841ab53</citedby><cites>FETCH-LOGICAL-c352t-80b6a2db0fca7b17367179c4c2c25c55a84c1e8f2d9b1c728dc738bf8c841ab53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.radphyschem.2008.08.010$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids></links><search><creatorcontrib>Divyashree, M.S.</creatorcontrib><creatorcontrib>Shamala, T.R.</creatorcontrib><title>Effect of gamma irradiation on cell lysis and polyhydroxyalkanoate produced by Bacillus flexus</title><title>Radiation physics and chemistry (Oxford, England : 1993)</title><description>Bacillus flexus cultivated on sucrose and sucrose with plant oil such as castor oil produced polyhydroxybutyrate (PHB), a homopolymer of polyhydroxyalkanoate (PHA) and PHA copolymer (containing hydroxybutyrate and hexanoate), respectively. Gamma irradiation of these cells (5–40
kGy) resulted in cell damage and aided in the isolation of 45% and 54% PHA on biomass weight, correspondingly. Molecular weight of PHB increased from 1.5×10
5 to 1.9×10
5 after irradiation (10
kGy), with marginal increase of tensile strength from 18 to 20
MPa. At the same irradiation dosage, PHA copolymer showed higher molecular weight increase from 1.7×10
5 to 2.3×10
5 and tensile strength from 20 to 35
MPa. GC, GC–MS, FTIR and
1H NMR were used for the characterization of PHA. Gamma irradiation seems to be a novel technique, to induce cross-linking and molecular weight increase of PHA copolymer and aid in easy extractability of intracellular PHA, simultaneously.</description><subject>1H NMR spectrum</subject><subject>Bacillus flexus</subject><subject>FTIR spectrum</subject><subject>Gamma irradiation</subject><subject>Polyhydroxyalkanoate copolymer</subject><subject>Polyhydroxybutyrate</subject><issn>0969-806X</issn><issn>1879-0895</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><recordid>eNqNkD9PwzAUxC0EEqXwHczC1mInTeyMUPFPqsQCEhOW82xTFycOdoKab4-jMjAinfSWd6e7H0KXlCwpoeX1bhmk6rZjhK1ulhkhfDmJkiM0o5xVC8Kr4hjNSFVWC07Kt1N0FuOOEMJ4kc_Q-50xGnrsDf6QTSOxDSnQyt76FieBdg67MdqIZatw5924HVXw-1G6T9l62WvcBa8G0ArXI76VYJ0bIjZO74d4jk6MdFFf_N45er2_e1k_LjbPD0_rm80C8iLrU7G6lJmqiQHJasryklFWwQoyyAooCslXQDU3mapqCizjCljOa8OBr6isi3yOrg65qcvXoGMvGhun7rLVfogiL3JGOK3SY3V4hOBjDNqILthGhlFQIiaiYif-EBUTUTGJkuRdH7w6Lfm2OogIVrdpuQ2JoVDe_iPlB_awh_E</recordid><startdate>20090201</startdate><enddate>20090201</enddate><creator>Divyashree, M.S.</creator><creator>Shamala, T.R.</creator><general>Elsevier Ltd</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope><scope>L7M</scope></search><sort><creationdate>20090201</creationdate><title>Effect of gamma irradiation on cell lysis and polyhydroxyalkanoate produced by Bacillus flexus</title><author>Divyashree, M.S. ; Shamala, T.R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c352t-80b6a2db0fca7b17367179c4c2c25c55a84c1e8f2d9b1c728dc738bf8c841ab53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>1H NMR spectrum</topic><topic>Bacillus flexus</topic><topic>FTIR spectrum</topic><topic>Gamma irradiation</topic><topic>Polyhydroxyalkanoate copolymer</topic><topic>Polyhydroxybutyrate</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Divyashree, M.S.</creatorcontrib><creatorcontrib>Shamala, T.R.</creatorcontrib><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><jtitle>Radiation physics and chemistry (Oxford, England : 1993)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Divyashree, M.S.</au><au>Shamala, T.R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of gamma irradiation on cell lysis and polyhydroxyalkanoate produced by Bacillus flexus</atitle><jtitle>Radiation physics and chemistry (Oxford, England : 1993)</jtitle><date>2009-02-01</date><risdate>2009</risdate><volume>78</volume><issue>2</issue><spage>147</spage><epage>152</epage><pages>147-152</pages><issn>0969-806X</issn><eissn>1879-0895</eissn><abstract>Bacillus flexus cultivated on sucrose and sucrose with plant oil such as castor oil produced polyhydroxybutyrate (PHB), a homopolymer of polyhydroxyalkanoate (PHA) and PHA copolymer (containing hydroxybutyrate and hexanoate), respectively. Gamma irradiation of these cells (5–40
kGy) resulted in cell damage and aided in the isolation of 45% and 54% PHA on biomass weight, correspondingly. Molecular weight of PHB increased from 1.5×10
5 to 1.9×10
5 after irradiation (10
kGy), with marginal increase of tensile strength from 18 to 20
MPa. At the same irradiation dosage, PHA copolymer showed higher molecular weight increase from 1.7×10
5 to 2.3×10
5 and tensile strength from 20 to 35
MPa. GC, GC–MS, FTIR and
1H NMR were used for the characterization of PHA. Gamma irradiation seems to be a novel technique, to induce cross-linking and molecular weight increase of PHA copolymer and aid in easy extractability of intracellular PHA, simultaneously.</abstract><pub>Elsevier Ltd</pub><doi>10.1016/j.radphyschem.2008.08.010</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0969-806X |
| ispartof | Radiation physics and chemistry (Oxford, England : 1993), 2009-02, Vol.78 (2), p.147-152 |
| issn | 0969-806X 1879-0895 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_35370819 |
| source | ScienceDirect Journals (5 years ago - present) |
| subjects | 1H NMR spectrum Bacillus flexus FTIR spectrum Gamma irradiation Polyhydroxyalkanoate copolymer Polyhydroxybutyrate |
| title | Effect of gamma irradiation on cell lysis and polyhydroxyalkanoate produced by Bacillus flexus |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-06T16%3A38%3A49IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Effect%20of%20gamma%20irradiation%20on%20cell%20lysis%20and%20polyhydroxyalkanoate%20produced%20by%20Bacillus%20flexus&rft.jtitle=Radiation%20physics%20and%20chemistry%20(Oxford,%20England%20:%201993)&rft.au=Divyashree,%20M.S.&rft.date=2009-02-01&rft.volume=78&rft.issue=2&rft.spage=147&rft.epage=152&rft.pages=147-152&rft.issn=0969-806X&rft.eissn=1879-0895&rft_id=info:doi/10.1016/j.radphyschem.2008.08.010&rft_dat=%3Cproquest_cross%3E35370819%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=35370819&rft_id=info:pmid/&rft_els_id=S0969806X0800203X&rfr_iscdi=true |