Knockout Rats via Embryo Microinjection of Zinc-Finger Nucleases

The toolbox of rat genetics currently lacks the ability to introduce site-directed, heritable mutations into the genome to create knockout animals. By using engineered zinc-finger nucleases (ZFNs) designed to target an integrated reporter and two endogenous rat genes, Immunoglobulin M (IgM) and Rab3...

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Veröffentlicht in:	Science (American Association for the Advancement of Science) 2009-07, Vol.325 (5939), p.433-433
Hauptverfasser:	Geurts, Aron M, Cost, Gregory J, Freyvert, Yevgeniy, Zeitler, Bryan, Miller, Jeffrey C, Choi, Vivian M, Jenkins, Shirin S, Wood, Adam, Cui, Xiaoxia, Meng, Xiangdong, Vincent, Anna, Lam, Stephen, Michalkiewicz, Mieczyslaw, Schilling, Rebecca, Foeckler, Jamie, Kalloway, Shawn, Weiler, Hartmut, Ménoret, Séverine, Anegon, Ignacio, Davis, Gregory D, Zhang, Lei, Rebar, Edward J, Gregory, Philip D, Urnov, Fyodor D, Jacob, Howard J, Buelow, Roland
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Base Sequence Biological and medical sciences Brevia Classical genetics, quantitative genetics, hybrids Deoxyribonucleic acid Disease models DNA Embryo, Mammalian Embryos Endodeoxyribonucleases - genetics Endodeoxyribonucleases - metabolism Feasibility Studies Female Fundamental and applied biological sciences. Psychology Gene Knockout Techniques Genetic mutation Genetic research Genetics of eukaryotes. Biological and molecular evolution Green Fluorescent Proteins Immunoglobulin M - genetics Inbreeding Male Messenger RNA Microinjections Molecular Sequence Data Mutagenesis, Site-Directed Mutation rab GTP-Binding Proteins - genetics Rats Ribonucleic acid RNA RNA, Messenger Rodents Vertebrata Zinc Fingers - genetics
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