SERS detection of indirect viral DNA capture using colloidal gold and methylene blue as a Raman label

An indirect capture model assay using colloidal Au nanoparticles is demonstrated for surface enhanced Raman scattering (SERS) spectroscopy detection of DNA. The sequence targeted for capture was derived from the West Nile Virus (WNV) RNA genome and selected on the basis of exhibiting minimal seconda...

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Veröffentlicht in:	Biosensors & bioelectronics 2009-12, Vol.25 (4), p.674-681
Hauptverfasser:	Harpster, Mark H., Zhang, Hao, Sankara-Warrier, Ajaya K., Ray, Bryan H., Ward, Timothy R., Kollmar, J. Pablo, Carron, Keith T., Mecham, James O., Corcoran, Robert C., Wilson, William C., Johnson, Patrick A.
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Biosensing Techniques - methods Biotechnology DNA DNA, Viral - analysis Fundamental and applied biological sciences. Psychology Gold Colloid - chemistry Indirect nucleic acid capture Methylene Blue Quartz crystal microbalance-dissipation Spectrum Analysis, Raman - methods Staining and Labeling - methods Surface enhanced Raman scattering West Nile Virus
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