A Method for Assessing Kinetic Changes of Histone H1 after Post-Translational Modifications

A Method for Assessing Kinetic Changes of Histone H1 after Post-Translational Modifications

Fluorescence Recovery After Photobleaching (FRAP) has become one of the main fluorescence microscopy techniques used to measure motion and interaction of nuclear proteins. In this work, we propose a mathematical method for analyzing sets of FRAP data to assess the effect of post-translational modifi...

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Hauptverfasser: Carrero, Gustavo, Raghuram, Nikhil, Th'ng, John, Hendzel, Michael
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Raghuram, Nikhil
Th'ng, John
Hendzel, Michael
description Fluorescence Recovery After Photobleaching (FRAP) has become one of the main fluorescence microscopy techniques used to measure motion and interaction of nuclear proteins. In this work, we propose a mathematical method for analyzing sets of FRAP data to assess the effect of post-translational modifications on the binding affinities of histone H1 proteins. The method is based on a model that accounts for weakly and strongly bound populations and comprises the estimation of kinetic parameters before and after post-translational modifications and the use of non-parametric statistics to analyze changes in the estimated parameters. The procedure is illustrated with sets of FRAP data of histone H1 binding to hyperacetylated chromatin.
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title A Method for Assessing Kinetic Changes of Histone H1 after Post-Translational Modifications
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