PVP-coated silver nanoparticles and silver ions induce reactive oxygen species, apoptosis and necrosis in THP-1 monocytes
The objective of the present study was to investigate the toxicity of silver nanoparticles (Ag NPs) in vitro. Silver ions (Ag+) have been used in medical treatments for decades whereas Ag NPs have been used in a variety of consumer products within recent years. This study was undertaken to compare t...
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| Veröffentlicht in: | Toxicology letters 2009-10, Vol.190 (2), p.156-162 |
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| creator | Foldbjerg, Rasmus Olesen, Ping Hougaard, Mads Dang, Duy Anh Hoffmann, Hans Jürgen Autrup, Herman |
| description | The objective of the present study was to investigate the toxicity of silver nanoparticles (Ag NPs)
in vitro. Silver ions (Ag+) have been used in medical treatments for decades whereas Ag NPs have been used in a variety of consumer products within recent years. This study was undertaken to compare the effect of well characterized, PVP-coated Ag NPs (69
nm
±
3
nm) and Ag+ in a human monocytic cell line (THP-1). Characterization of the Ag NPs was conducted in both stock suspension and cell media with or without serum and antibiotics. By using the flowcytometric annexin V/propidium iodide (PI) assay, both Ag NPs and Ag+ were shown to induce apoptosis and necrosis in THP-1 cells depending on dose and exposure time. Furthermore, the presence of apoptosis could be confirmed by the TUNEL method. A number of studies have implicated the production of reactive oxygen species (ROS) in cytotoxicity mediated by NPs. We used the fluorogenic probe, 2′,7′-dichlorofluorescein to assess the levels of intracellular ROS during exposure to Ag NPs and Ag+. A drastic increase in ROS levels could be detected after 6–24
h suggesting that oxidative stress is an important mediator of cytotoxicity caused by Ag NPs and Ag+. |
| doi_str_mv | 10.1016/j.toxlet.2009.07.009 |
| format | Article |
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in vitro. Silver ions (Ag+) have been used in medical treatments for decades whereas Ag NPs have been used in a variety of consumer products within recent years. This study was undertaken to compare the effect of well characterized, PVP-coated Ag NPs (69
nm
±
3
nm) and Ag+ in a human monocytic cell line (THP-1). Characterization of the Ag NPs was conducted in both stock suspension and cell media with or without serum and antibiotics. By using the flowcytometric annexin V/propidium iodide (PI) assay, both Ag NPs and Ag+ were shown to induce apoptosis and necrosis in THP-1 cells depending on dose and exposure time. Furthermore, the presence of apoptosis could be confirmed by the TUNEL method. A number of studies have implicated the production of reactive oxygen species (ROS) in cytotoxicity mediated by NPs. We used the fluorogenic probe, 2′,7′-dichlorofluorescein to assess the levels of intracellular ROS during exposure to Ag NPs and Ag+. A drastic increase in ROS levels could be detected after 6–24
h suggesting that oxidative stress is an important mediator of cytotoxicity caused by Ag NPs and Ag+.</description><identifier>ISSN: 0378-4274</identifier><identifier>EISSN: 1879-3169</identifier><identifier>DOI: 10.1016/j.toxlet.2009.07.009</identifier><identifier>PMID: 19607894</identifier><identifier>CODEN: TOLED5</identifier><language>eng</language><publisher>Shannon: Elsevier Ireland Ltd</publisher><subject>Annexin A5 - metabolism ; Apoptosis ; Apoptosis - drug effects ; Biological and medical sciences ; Cell Line, Tumor ; Chemical and industrial products toxicology. Toxic occupational diseases ; Flow Cytometry ; Fluorescent Dyes ; Humans ; In Situ Nick-End Labeling ; Light ; Medical sciences ; Metals and various inorganic compounds ; Monocytes ; Monocytes - drug effects ; Monocytes - metabolism ; Monocytes - pathology ; Nanoparticles ; Necrosis - pathology ; Particle Size ; Polyvinyl Chloride - pharmacology ; Reactive oxygen species ; Reactive Oxygen Species - metabolism ; Scattering, Radiation ; Silver ; Silver - pharmacology ; Toxicology</subject><ispartof>Toxicology letters, 2009-10, Vol.190 (2), p.156-162</ispartof><rights>2009 Elsevier Ireland Ltd</rights><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c452t-9e94ebfe5e3f220a407e4084e1651af24234d924aad35ce33087292b57b092403</citedby><cites>FETCH-LOGICAL-c452t-9e94ebfe5e3f220a407e4084e1651af24234d924aad35ce33087292b57b092403</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0378427409012211$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=22241532$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19607894$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Foldbjerg, Rasmus</creatorcontrib><creatorcontrib>Olesen, Ping</creatorcontrib><creatorcontrib>Hougaard, Mads</creatorcontrib><creatorcontrib>Dang, Duy Anh</creatorcontrib><creatorcontrib>Hoffmann, Hans Jürgen</creatorcontrib><creatorcontrib>Autrup, Herman</creatorcontrib><title>PVP-coated silver nanoparticles and silver ions induce reactive oxygen species, apoptosis and necrosis in THP-1 monocytes</title><title>Toxicology letters</title><addtitle>Toxicol Lett</addtitle><description>The objective of the present study was to investigate the toxicity of silver nanoparticles (Ag NPs)
in vitro. Silver ions (Ag+) have been used in medical treatments for decades whereas Ag NPs have been used in a variety of consumer products within recent years. This study was undertaken to compare the effect of well characterized, PVP-coated Ag NPs (69
nm
±
3
nm) and Ag+ in a human monocytic cell line (THP-1). Characterization of the Ag NPs was conducted in both stock suspension and cell media with or without serum and antibiotics. By using the flowcytometric annexin V/propidium iodide (PI) assay, both Ag NPs and Ag+ were shown to induce apoptosis and necrosis in THP-1 cells depending on dose and exposure time. Furthermore, the presence of apoptosis could be confirmed by the TUNEL method. A number of studies have implicated the production of reactive oxygen species (ROS) in cytotoxicity mediated by NPs. We used the fluorogenic probe, 2′,7′-dichlorofluorescein to assess the levels of intracellular ROS during exposure to Ag NPs and Ag+. A drastic increase in ROS levels could be detected after 6–24
h suggesting that oxidative stress is an important mediator of cytotoxicity caused by Ag NPs and Ag+.</description><subject>Annexin A5 - metabolism</subject><subject>Apoptosis</subject><subject>Apoptosis - drug effects</subject><subject>Biological and medical sciences</subject><subject>Cell Line, Tumor</subject><subject>Chemical and industrial products toxicology. Toxic occupational diseases</subject><subject>Flow Cytometry</subject><subject>Fluorescent Dyes</subject><subject>Humans</subject><subject>In Situ Nick-End Labeling</subject><subject>Light</subject><subject>Medical sciences</subject><subject>Metals and various inorganic compounds</subject><subject>Monocytes</subject><subject>Monocytes - drug effects</subject><subject>Monocytes - metabolism</subject><subject>Monocytes - pathology</subject><subject>Nanoparticles</subject><subject>Necrosis - pathology</subject><subject>Particle Size</subject><subject>Polyvinyl Chloride - pharmacology</subject><subject>Reactive oxygen species</subject><subject>Reactive Oxygen Species - metabolism</subject><subject>Scattering, Radiation</subject><subject>Silver</subject><subject>Silver - pharmacology</subject><subject>Toxicology</subject><issn>0378-4274</issn><issn>1879-3169</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU9v1DAQxS0EotvCN0DIFziRMP6TOL4gVRW0SJXYQ-FqeZ0J8iprB9u76n57smRVbnB6mvHvjcbzCHnDoGbA2o_busTHEUvNAXQNqp7lGVmxTulKsFY_JysQqqskV_KCXOa8BYBWts1LcsF0C6rTckWO6x_rykVbsKfZjwdMNNgQJ5uKdyNmasPTg48hUx_6vUOa0LriD0jj4_EnBpondB7zB2qnOJWY_eIM6NKfwgf6cLeuGN3FEN2xYH5FXgx2zPj6rFfk-5fPDzd31f2326831_eVkw0vlUYtcTNgg2LgHKwEhRI6iaxtmB245EL2mktre9E4FAI6xTXfNGoDcxvEFXm_zJ1S_LXHXMzOZ4fjaAPGfTZCKi2FFv8FOSjdiK6ZQbmAp7_lhIOZkt_ZdDQMzCkbszVLNuaUjQFlZpltb8_z95sd9n9N5zBm4N0ZsNnZcUg2OJ-fOM65ZI3gM_dp4XA-28FjMnm-fXDY-4SumD76f2_yGyUvr8g</recordid><startdate>20091028</startdate><enddate>20091028</enddate><creator>Foldbjerg, Rasmus</creator><creator>Olesen, Ping</creator><creator>Hougaard, Mads</creator><creator>Dang, Duy Anh</creator><creator>Hoffmann, Hans Jürgen</creator><creator>Autrup, Herman</creator><general>Elsevier Ireland Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7ST</scope><scope>7T5</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>SOI</scope><scope>KR7</scope></search><sort><creationdate>20091028</creationdate><title>PVP-coated silver nanoparticles and silver ions induce reactive oxygen species, apoptosis and necrosis in THP-1 monocytes</title><author>Foldbjerg, Rasmus ; Olesen, Ping ; Hougaard, Mads ; Dang, Duy Anh ; Hoffmann, Hans Jürgen ; Autrup, Herman</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c452t-9e94ebfe5e3f220a407e4084e1651af24234d924aad35ce33087292b57b092403</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Annexin A5 - metabolism</topic><topic>Apoptosis</topic><topic>Apoptosis - drug effects</topic><topic>Biological and medical sciences</topic><topic>Cell Line, Tumor</topic><topic>Chemical and industrial products toxicology. Toxic occupational diseases</topic><topic>Flow Cytometry</topic><topic>Fluorescent Dyes</topic><topic>Humans</topic><topic>In Situ Nick-End Labeling</topic><topic>Light</topic><topic>Medical sciences</topic><topic>Metals and various inorganic compounds</topic><topic>Monocytes</topic><topic>Monocytes - drug effects</topic><topic>Monocytes - metabolism</topic><topic>Monocytes - pathology</topic><topic>Nanoparticles</topic><topic>Necrosis - pathology</topic><topic>Particle Size</topic><topic>Polyvinyl Chloride - pharmacology</topic><topic>Reactive oxygen species</topic><topic>Reactive Oxygen Species - metabolism</topic><topic>Scattering, Radiation</topic><topic>Silver</topic><topic>Silver - pharmacology</topic><topic>Toxicology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Foldbjerg, Rasmus</creatorcontrib><creatorcontrib>Olesen, Ping</creatorcontrib><creatorcontrib>Hougaard, Mads</creatorcontrib><creatorcontrib>Dang, Duy Anh</creatorcontrib><creatorcontrib>Hoffmann, Hans Jürgen</creatorcontrib><creatorcontrib>Autrup, Herman</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Environment Abstracts</collection><collection>Immunology Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environment Abstracts</collection><collection>Civil Engineering Abstracts</collection><jtitle>Toxicology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Foldbjerg, Rasmus</au><au>Olesen, Ping</au><au>Hougaard, Mads</au><au>Dang, Duy Anh</au><au>Hoffmann, Hans Jürgen</au><au>Autrup, Herman</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>PVP-coated silver nanoparticles and silver ions induce reactive oxygen species, apoptosis and necrosis in THP-1 monocytes</atitle><jtitle>Toxicology letters</jtitle><addtitle>Toxicol Lett</addtitle><date>2009-10-28</date><risdate>2009</risdate><volume>190</volume><issue>2</issue><spage>156</spage><epage>162</epage><pages>156-162</pages><issn>0378-4274</issn><eissn>1879-3169</eissn><coden>TOLED5</coden><abstract>The objective of the present study was to investigate the toxicity of silver nanoparticles (Ag NPs)
in vitro. Silver ions (Ag+) have been used in medical treatments for decades whereas Ag NPs have been used in a variety of consumer products within recent years. This study was undertaken to compare the effect of well characterized, PVP-coated Ag NPs (69
nm
±
3
nm) and Ag+ in a human monocytic cell line (THP-1). Characterization of the Ag NPs was conducted in both stock suspension and cell media with or without serum and antibiotics. By using the flowcytometric annexin V/propidium iodide (PI) assay, both Ag NPs and Ag+ were shown to induce apoptosis and necrosis in THP-1 cells depending on dose and exposure time. Furthermore, the presence of apoptosis could be confirmed by the TUNEL method. A number of studies have implicated the production of reactive oxygen species (ROS) in cytotoxicity mediated by NPs. We used the fluorogenic probe, 2′,7′-dichlorofluorescein to assess the levels of intracellular ROS during exposure to Ag NPs and Ag+. A drastic increase in ROS levels could be detected after 6–24
h suggesting that oxidative stress is an important mediator of cytotoxicity caused by Ag NPs and Ag+.</abstract><cop>Shannon</cop><pub>Elsevier Ireland Ltd</pub><pmid>19607894</pmid><doi>10.1016/j.toxlet.2009.07.009</doi><tpages>7</tpages></addata></record> |
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| ispartof | Toxicology letters, 2009-10, Vol.190 (2), p.156-162 |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Annexin A5 - metabolism Apoptosis Apoptosis - drug effects Biological and medical sciences Cell Line, Tumor Chemical and industrial products toxicology. Toxic occupational diseases Flow Cytometry Fluorescent Dyes Humans In Situ Nick-End Labeling Light Medical sciences Metals and various inorganic compounds Monocytes Monocytes - drug effects Monocytes - metabolism Monocytes - pathology Nanoparticles Necrosis - pathology Particle Size Polyvinyl Chloride - pharmacology Reactive oxygen species Reactive Oxygen Species - metabolism Scattering, Radiation Silver Silver - pharmacology Toxicology |
| title | PVP-coated silver nanoparticles and silver ions induce reactive oxygen species, apoptosis and necrosis in THP-1 monocytes |
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