In vitro propagation of plant virus using different forms of plant tissue culture and modes of culture operation

Plant virus accumulation was investigated in vitro using three different forms of plant tissue culture. Suspended cells, hairy roots and shooty teratomas of Nicotiana benthamiana were infected with tobacco mosaic virus (TMV) using the same initial virus:biomass ratio. Viral infection did not affect...

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Veröffentlicht in:	Journal of biotechnology 2009-09, Vol.143 (3), p.198-206
Hauptverfasser:	Shih, Sharon M.-H., Doran, Pauline M.
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Biomass Biotechnology cell suspension culture Culture Culture Media Drying Fundamental and applied biological sciences. Psychology Green Fluorescent Proteins - metabolism Hairy roots In vitro testing Mathematical analysis Nicotiana - growth & development Nicotiana - metabolism Nicotiana - virology Nicotiana benthamiana Plant Extracts Plant Roots - growth & development Plant Roots - metabolism Plant Roots - virology plant tumors Plant virus Plants (organisms) Proteins Roots shoots shooty teratomas tissue culture Tissue Culture Techniques - methods Tobacco mosaic virus Tobacco mosaic virus (TMV) Tobacco Mosaic Virus - genetics Tobacco Mosaic Virus - growth & development Tobacco Mosaic Virus - pathogenicity Transient expression virus replication
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