Immobilization–stabilization of the lipase from Thermomyces lanuginosus: Critical role of chemical amination

This paper describes the immobilization and stabilization of the lipase from Thermomyces lanuginosus (TLL) on glyoxyl agarose. Enzymes attach to this support only by the reaction between several aldehyde groups of the support and several Lys residues on the external surface of the enzyme molecules a...

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Veröffentlicht in:	Process biochemistry (1991) 2009-09, Vol.44 (9), p.963-968
Hauptverfasser:	Rodrigues, Rafael C., Godoy, Cesar A., Volpato, Giandra, Ayub, Marco A.Z., Fernandez-Lafuente, Roberto, Guisan, Jose M.
Format:	Artikel
Sprache:	eng
Schlagworte:	Chemical amination Enzyme immobilization Enzyme stabilization Glyoxyl agarose Multipoint covalent attachment Thermomyces lanuginosus Thermomyces lanuginosus lipase
Online-Zugang:	Volltext
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