Isolation of Cordyceps ophioglossoides L2 from Fruit Body and Optimization of Fermentation Conditions for Its Mycelial Growth
A strain isolated from the fruiting body of a fungus parasitized on Elaphomyces was identified as Cordyceps ophioglossoides based on the morphological characteristics and the analysis of ITS-5.8s rDNA sequence. The optimal medium, composition （g·L^-1）, containing sucrose 66.0, yeast powder 10.0, sil...
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| Veröffentlicht in: | Chinese journal of chemical engineering 2009-04, Vol.17 (2), p.278-285 |
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| creator | 许勤勤 吕龙贤 陈少云 郑静 郑高利 李永泉 |
| description | A strain isolated from the fruiting body of a fungus parasitized on Elaphomyces was identified as Cordyceps ophioglossoides based on the morphological characteristics and the analysis of ITS-5.8s rDNA sequence. The optimal medium, composition （g·L^-1）, containing sucrose 66.0, yeast powder 10.0, silkworm chrysalises digest 30.0, MgSO4· 7H2O 0.4, and KH2PO4 0.4, Was found using fractional factorial design ancl a central composite design, and the optimization of cultural conditions obtained a result of seed age 6 days, inoculum size 6% （by volume）, initial pH 5.6, temperature 24℃, shaking speed 160 ·＇min^-1 by one-factor-at-a-time method. The maximum biomass reached about 20.2 g·L^-1 after 90 hours culture under the optimal conditions. Elementary nharmaeclogical actlwtties showed that mycelia of C. ophioglossoides L2 from submerged culture promoted Uterus growth in estrogen- depleted mice. In the 15-litre scale-up fermentation, the mycelial biomass was around 19.1 g·L^-1, indicating a promising prospect for this biotechnoloagy and the potency to develoo its medical value. |
| doi_str_mv | 10.1016/S1004-9541(08)60206-2 |
| format | Article |
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In the 15-litre scale-up fermentation, the mycelial biomass was around 19.1 g·L^-1, indicating a promising prospect for this biotechnoloagy and the potency to develoo its medical value.</description><identifier>ISSN: 1004-9541</identifier><identifier>EISSN: 2210-321X</identifier><identifier>DOI: 10.1016/S1004-9541(08)60206-2</identifier><language>eng</language><publisher>Elsevier B.V</publisher><subject>Bombyx mori ; central composite design ; Cordyceps ophioglossoides ; fermentation ; fractional factorial design ; identification ; optimization ; 冬虫夏草 ; 发酵条件 ; 子实体 ; 菌丝生物量 ; 菌丝生长</subject><ispartof>Chinese journal of chemical engineering, 2009-04, Vol.17 (2), p.278-285</ispartof><rights>2009 Chemical Industry and Engineering Society of China (CIESC) and Chemical Industry Press (CIP)</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c429t-c11d9c3fccece5fbf8f70c471015c69c020f3dd5c08c431bd316aebe6d29a1b63</citedby><cites>FETCH-LOGICAL-c429t-c11d9c3fccece5fbf8f70c471015c69c020f3dd5c08c431bd316aebe6d29a1b63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/84275X/84275X.jpg</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S1004-9541(08)60206-2$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids></links><search><creatorcontrib>许勤勤 吕龙贤 陈少云 郑静 郑高利 李永泉</creatorcontrib><title>Isolation of Cordyceps ophioglossoides L2 from Fruit Body and Optimization of Fermentation Conditions for Its Mycelial Growth</title><title>Chinese journal of chemical engineering</title><addtitle>Chinese Journal of Chemical Engineering</addtitle><description>A strain isolated from the fruiting body of a fungus parasitized on Elaphomyces was identified as Cordyceps ophioglossoides based on the morphological characteristics and the analysis of ITS-5.8s rDNA sequence. The optimal medium, composition （g·L^-1）, containing sucrose 66.0, yeast powder 10.0, silkworm chrysalises digest 30.0, MgSO4· 7H2O 0.4, and KH2PO4 0.4, Was found using fractional factorial design ancl a central composite design, and the optimization of cultural conditions obtained a result of seed age 6 days, inoculum size 6% （by volume）, initial pH 5.6, temperature 24℃, shaking speed 160 ·＇min^-1 by one-factor-at-a-time method. The maximum biomass reached about 20.2 g·L^-1 after 90 hours culture under the optimal conditions. Elementary nharmaeclogical actlwtties showed that mycelia of C. ophioglossoides L2 from submerged culture promoted Uterus growth in estrogen- depleted mice. In the 15-litre scale-up fermentation, the mycelial biomass was around 19.1 g·L^-1, indicating a promising prospect for this biotechnoloagy and the potency to develoo its medical value.</description><subject>Bombyx mori</subject><subject>central composite design</subject><subject>Cordyceps ophioglossoides</subject><subject>fermentation</subject><subject>fractional factorial design</subject><subject>identification</subject><subject>optimization</subject><subject>冬虫夏草</subject><subject>发酵条件</subject><subject>子实体</subject><subject>菌丝生物量</subject><subject>菌丝生长</subject><issn>1004-9541</issn><issn>2210-321X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><recordid>eNqNkU9vEzEQxS1EJULLR0CyOCA4LPjPrnd9QhCREilVDwWJm-W1x4lhd721HVCQ-O44TdVrOXks_ebNzHsIvaTkHSVUvL-hhNSVbGr6hnRvBWFEVOwJWjBGScUZ_f4ULR6QZ-h5Sj9IgTraLdDfdQqDzj5MODi8DNEeDMwJh3nnw3YIKQVvIeENwy6GEa_i3mf8KdgD1pPF13P2o__zILCCOMKUT_9lmKw_Vgm7EPE6J3xV1AevB3wZw--8u0BnTg8JXty_5-jb6vPX5Zdqc325Xn7cVKZmMleGUisNd8aAgcb1rnMtMXVbrm-MkKYc47i1jSGdqTntLadCQw_CMqlpL_g5en3SnWO43UPKavSpbDLoCcI-KV7XjZSS_AfI22K5fBQs9greibaAzQk0sZgZwak5-lHHg6JEHeNTd_GpYzaKdOouPsVK34dTHxRffnmIKhkPkwHrI5isbPCPKry6n7wL0_bWT1vVa_PT-QEUJ7SWhLb8H-_vr4U</recordid><startdate>20090401</startdate><enddate>20090401</enddate><creator>许勤勤 吕龙贤 陈少云 郑静 郑高利 李永泉</creator><general>Elsevier B.V</general><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W92</scope><scope>~WA</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>7U5</scope><scope>L7M</scope></search><sort><creationdate>20090401</creationdate><title>Isolation of Cordyceps ophioglossoides L2 from Fruit Body and Optimization of Fermentation Conditions for Its Mycelial Growth</title><author>许勤勤 吕龙贤 陈少云 郑静 郑高利 李永泉</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c429t-c11d9c3fccece5fbf8f70c471015c69c020f3dd5c08c431bd316aebe6d29a1b63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Bombyx mori</topic><topic>central composite design</topic><topic>Cordyceps ophioglossoides</topic><topic>fermentation</topic><topic>fractional factorial design</topic><topic>identification</topic><topic>optimization</topic><topic>冬虫夏草</topic><topic>发酵条件</topic><topic>子实体</topic><topic>菌丝生物量</topic><topic>菌丝生长</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>许勤勤 吕龙贤 陈少云 郑静 郑高利 李永泉</creatorcontrib><collection>维普_期刊</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>维普中文期刊数据库</collection><collection>中文科技期刊数据库-工程技术</collection><collection>中文科技期刊数据库- 镜像站点</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><jtitle>Chinese journal of chemical engineering</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>许勤勤 吕龙贤 陈少云 郑静 郑高利 李永泉</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation of Cordyceps ophioglossoides L2 from Fruit Body and Optimization of Fermentation Conditions for Its Mycelial Growth</atitle><jtitle>Chinese journal of chemical engineering</jtitle><addtitle>Chinese Journal of Chemical Engineering</addtitle><date>2009-04-01</date><risdate>2009</risdate><volume>17</volume><issue>2</issue><spage>278</spage><epage>285</epage><pages>278-285</pages><issn>1004-9541</issn><eissn>2210-321X</eissn><abstract>A strain isolated from the fruiting body of a fungus parasitized on Elaphomyces was identified as Cordyceps ophioglossoides based on the morphological characteristics and the analysis of ITS-5.8s rDNA sequence. The optimal medium, composition （g·L^-1）, containing sucrose 66.0, yeast powder 10.0, silkworm chrysalises digest 30.0, MgSO4· 7H2O 0.4, and KH2PO4 0.4, Was found using fractional factorial design ancl a central composite design, and the optimization of cultural conditions obtained a result of seed age 6 days, inoculum size 6% （by volume）, initial pH 5.6, temperature 24℃, shaking speed 160 ·＇min^-1 by one-factor-at-a-time method. The maximum biomass reached about 20.2 g·L^-1 after 90 hours culture under the optimal conditions. Elementary nharmaeclogical actlwtties showed that mycelia of C. ophioglossoides L2 from submerged culture promoted Uterus growth in estrogen- depleted mice. In the 15-litre scale-up fermentation, the mycelial biomass was around 19.1 g·L^-1, indicating a promising prospect for this biotechnoloagy and the potency to develoo its medical value.</abstract><pub>Elsevier B.V</pub><doi>10.1016/S1004-9541(08)60206-2</doi><tpages>8</tpages></addata></record> |
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| subjects | Bombyx mori central composite design Cordyceps ophioglossoides fermentation fractional factorial design identification optimization 冬虫夏草 发酵条件 子实体 菌丝生物量 菌丝生长 |
| title | Isolation of Cordyceps ophioglossoides L2 from Fruit Body and Optimization of Fermentation Conditions for Its Mycelial Growth |
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