Analysis of dissolved microcystins in surface water samples from Kovada Lake, Turkey
Dissolved (extracellular) microcystin (MC) concentrations were determined at 3 sampling stations on Lake Kovada, Turkey. The dominant species of cyanobacteria found in August and September of 2006 were Microcystis aeruginosa, Synechococcus sp., Phormidium limosum, Phormidium formosa and Planktothrix...
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| Veröffentlicht in: | The Science of the total environment 2009-06, Vol.407 (13), p.4038-4046 |
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| creator | Gurbuz, Fatma Metcalf, James S. Karahan, Aynur Gul Codd, Geoffrey A. |
| description | Dissolved (extracellular) microcystin (MC) concentrations were determined at 3 sampling stations on Lake Kovada, Turkey. The dominant species of cyanobacteria found in August and September of 2006 were
Microcystis aeruginosa,
Synechococcus sp.,
Phormidium limosum, Phormidium formosa and Planktothrix limnetica. MC concentrations in water were measured by ELISA and MC variants were examined by HPLC-PDA. Quantitative analysis by HPLC indicated that five MC variants (MC–LR, –RR, –LA, –LW, –LF) were identified in water samples from Kovada Lake. The maximum concentration of dissolved MC–LW was 98.9 µg l
−
1
in October. MC–LR was only detected in May at a concentration of 0.5 µg l
−
1
. The cross reactivity of the antibody (MC10E7) to variants such as MC–LA MC–LW & MC–LF was low. Hence the results determined by ELISA were lower than those determined by HPLC in September and October samples due to differences in the specificity of the antibody to MC variants. Total extraceullar MCs was quantified by ELISA and ranged from 0.73 to 48.5 µg MC–LR equivalents l
−
1
, which in some cases exceeded the WHO provisional Guideline Value for MC–LR in drinking water. This study confirms that the lakes of Turkey should be monitored for toxic cyanobacteria and for MCs to avoid or reduce the potential exposure of people to these health hazards. |
| doi_str_mv | 10.1016/j.scitotenv.2009.02.039 |
| format | Article |
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Microcystis aeruginosa,
Synechococcus sp.,
Phormidium limosum, Phormidium formosa and Planktothrix limnetica. MC concentrations in water were measured by ELISA and MC variants were examined by HPLC-PDA. Quantitative analysis by HPLC indicated that five MC variants (MC–LR, –RR, –LA, –LW, –LF) were identified in water samples from Kovada Lake. The maximum concentration of dissolved MC–LW was 98.9 µg l
−
1
in October. MC–LR was only detected in May at a concentration of 0.5 µg l
−
1
. The cross reactivity of the antibody (MC10E7) to variants such as MC–LA MC–LW & MC–LF was low. Hence the results determined by ELISA were lower than those determined by HPLC in September and October samples due to differences in the specificity of the antibody to MC variants. Total extraceullar MCs was quantified by ELISA and ranged from 0.73 to 48.5 µg MC–LR equivalents l
−
1
, which in some cases exceeded the WHO provisional Guideline Value for MC–LR in drinking water. This study confirms that the lakes of Turkey should be monitored for toxic cyanobacteria and for MCs to avoid or reduce the potential exposure of people to these health hazards.</description><identifier>ISSN: 0048-9697</identifier><identifier>EISSN: 1879-1026</identifier><identifier>DOI: 10.1016/j.scitotenv.2009.02.039</identifier><identifier>PMID: 19395066</identifier><identifier>CODEN: STENDL</identifier><language>eng</language><publisher>Kidlington: Elsevier B.V</publisher><subject>Animal and plant ecology ; Animal, plant and microbial ecology ; Antibodies ; Biological and medical sciences ; Biomass ; Chromatography, High Pressure Liquid ; Cyanobacteria ; Cyanobacteria - chemistry ; Cyanobacteria - isolation & purification ; Dissolution ; ELISA ; Enzyme-Linked Immunosorbent Assay ; Fresh Water - chemistry ; Fresh water ecosystems ; Freshwater ; Fundamental and applied biological sciences. Psychology ; Guidelines ; HPLC analysis ; Kovada Lake ; Lakes ; Microcystin ; Microcystins - analysis ; Microcystis ; Microcystis aeruginosa ; Phormidium ; Planktothrix ; Quantitative analysis ; Solubility ; Stations ; Synechococcus ; Synecology ; Turkey ; Water Microbiology</subject><ispartof>The Science of the total environment, 2009-06, Vol.407 (13), p.4038-4046</ispartof><rights>2009</rights><rights>2009 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c551t-fc7208f73bee24fa9a06d2e5e6ee336771a69a56154feadde7433234dc479eb93</citedby><cites>FETCH-LOGICAL-c551t-fc7208f73bee24fa9a06d2e5e6ee336771a69a56154feadde7433234dc479eb93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0048969709002113$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21589071$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19395066$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gurbuz, Fatma</creatorcontrib><creatorcontrib>Metcalf, James S.</creatorcontrib><creatorcontrib>Karahan, Aynur Gul</creatorcontrib><creatorcontrib>Codd, Geoffrey A.</creatorcontrib><title>Analysis of dissolved microcystins in surface water samples from Kovada Lake, Turkey</title><title>The Science of the total environment</title><addtitle>Sci Total Environ</addtitle><description>Dissolved (extracellular) microcystin (MC) concentrations were determined at 3 sampling stations on Lake Kovada, Turkey. The dominant species of cyanobacteria found in August and September of 2006 were
Microcystis aeruginosa,
Synechococcus sp.,
Phormidium limosum, Phormidium formosa and Planktothrix limnetica. MC concentrations in water were measured by ELISA and MC variants were examined by HPLC-PDA. Quantitative analysis by HPLC indicated that five MC variants (MC–LR, –RR, –LA, –LW, –LF) were identified in water samples from Kovada Lake. The maximum concentration of dissolved MC–LW was 98.9 µg l
−
1
in October. MC–LR was only detected in May at a concentration of 0.5 µg l
−
1
. The cross reactivity of the antibody (MC10E7) to variants such as MC–LA MC–LW & MC–LF was low. Hence the results determined by ELISA were lower than those determined by HPLC in September and October samples due to differences in the specificity of the antibody to MC variants. Total extraceullar MCs was quantified by ELISA and ranged from 0.73 to 48.5 µg MC–LR equivalents l
−
1
, which in some cases exceeded the WHO provisional Guideline Value for MC–LR in drinking water. This study confirms that the lakes of Turkey should be monitored for toxic cyanobacteria and for MCs to avoid or reduce the potential exposure of people to these health hazards.</description><subject>Animal and plant ecology</subject><subject>Animal, plant and microbial ecology</subject><subject>Antibodies</subject><subject>Biological and medical sciences</subject><subject>Biomass</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Cyanobacteria</subject><subject>Cyanobacteria - chemistry</subject><subject>Cyanobacteria - isolation & purification</subject><subject>Dissolution</subject><subject>ELISA</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Fresh Water - chemistry</subject><subject>Fresh water ecosystems</subject><subject>Freshwater</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Guidelines</subject><subject>HPLC analysis</subject><subject>Kovada Lake</subject><subject>Lakes</subject><subject>Microcystin</subject><subject>Microcystins - analysis</subject><subject>Microcystis</subject><subject>Microcystis aeruginosa</subject><subject>Phormidium</subject><subject>Planktothrix</subject><subject>Quantitative analysis</subject><subject>Solubility</subject><subject>Stations</subject><subject>Synechococcus</subject><subject>Synecology</subject><subject>Turkey</subject><subject>Water Microbiology</subject><issn>0048-9697</issn><issn>1879-1026</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1vEzEQhi0EomnhL1Bf4MQu_lo7PkZV-RCROJCeLcceI6e762Dvpsq_x1GicuzI0lyeeWf8IHRLSUsJlV92bXFxShOMh5YRolvCWsL1K7SgS6UbSph8jRaEiGWjpVZX6LqUHamllvQtuqKa645IuUCb1Wj7Y4kFp4B9LCX1B_B4iC4ndyxTHAuOIy5zDtYBfrITZFzssO-h4JDTgH-mg_UWr-0jfMabOT_C8R16E2xf4P2l36CHr_ebu-_N-te3H3erdeO6jk5NcIqRZVB8C8BEsNoS6Rl0IAE4l0pRK7XtJO1EAOs9KME548I7oTRsNb9Bn865-5z-zlAmM8TioO_tCGkuhgshVH0vgozUaKlPieoM1u-XkiGYfY6DzUdDiTmZNzvzbN6czBvCTDVfJz9cVszbAfz_uYvqCny8ALY424dsRxfLM8dot9RE0crdnrlgk7F_cmUefjNCed3OiWSiEqszAdXtIUI-nQSjAx8zuMn4FF889x93g7Ai</recordid><startdate>20090615</startdate><enddate>20090615</enddate><creator>Gurbuz, Fatma</creator><creator>Metcalf, James S.</creator><creator>Karahan, Aynur Gul</creator><creator>Codd, Geoffrey A.</creator><general>Elsevier B.V</general><general>[Amsterdam; New York]: Elsevier Science</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QH</scope><scope>7TV</scope><scope>7U7</scope><scope>7UA</scope><scope>C1K</scope><scope>F1W</scope><scope>H95</scope><scope>H97</scope><scope>L.G</scope><scope>M7N</scope><scope>7SU</scope><scope>8FD</scope><scope>FR3</scope><scope>KR7</scope></search><sort><creationdate>20090615</creationdate><title>Analysis of dissolved microcystins in surface water samples from Kovada Lake, Turkey</title><author>Gurbuz, Fatma ; Metcalf, James S. ; Karahan, Aynur Gul ; Codd, Geoffrey A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c551t-fc7208f73bee24fa9a06d2e5e6ee336771a69a56154feadde7433234dc479eb93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Animal and plant ecology</topic><topic>Animal, plant and microbial ecology</topic><topic>Antibodies</topic><topic>Biological and medical sciences</topic><topic>Biomass</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Cyanobacteria</topic><topic>Cyanobacteria - chemistry</topic><topic>Cyanobacteria - isolation & purification</topic><topic>Dissolution</topic><topic>ELISA</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Fresh Water - chemistry</topic><topic>Fresh water ecosystems</topic><topic>Freshwater</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Guidelines</topic><topic>HPLC analysis</topic><topic>Kovada Lake</topic><topic>Lakes</topic><topic>Microcystin</topic><topic>Microcystins - analysis</topic><topic>Microcystis</topic><topic>Microcystis aeruginosa</topic><topic>Phormidium</topic><topic>Planktothrix</topic><topic>Quantitative analysis</topic><topic>Solubility</topic><topic>Stations</topic><topic>Synechococcus</topic><topic>Synecology</topic><topic>Turkey</topic><topic>Water Microbiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gurbuz, Fatma</creatorcontrib><creatorcontrib>Metcalf, James S.</creatorcontrib><creatorcontrib>Karahan, Aynur Gul</creatorcontrib><creatorcontrib>Codd, Geoffrey A.</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aqualine</collection><collection>Pollution Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Water Resources Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Environmental Engineering Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Civil Engineering Abstracts</collection><jtitle>The Science of the total environment</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gurbuz, Fatma</au><au>Metcalf, James S.</au><au>Karahan, Aynur Gul</au><au>Codd, Geoffrey A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Analysis of dissolved microcystins in surface water samples from Kovada Lake, Turkey</atitle><jtitle>The Science of the total environment</jtitle><addtitle>Sci Total Environ</addtitle><date>2009-06-15</date><risdate>2009</risdate><volume>407</volume><issue>13</issue><spage>4038</spage><epage>4046</epage><pages>4038-4046</pages><issn>0048-9697</issn><eissn>1879-1026</eissn><coden>STENDL</coden><abstract>Dissolved (extracellular) microcystin (MC) concentrations were determined at 3 sampling stations on Lake Kovada, Turkey. The dominant species of cyanobacteria found in August and September of 2006 were
Microcystis aeruginosa,
Synechococcus sp.,
Phormidium limosum, Phormidium formosa and Planktothrix limnetica. MC concentrations in water were measured by ELISA and MC variants were examined by HPLC-PDA. Quantitative analysis by HPLC indicated that five MC variants (MC–LR, –RR, –LA, –LW, –LF) were identified in water samples from Kovada Lake. The maximum concentration of dissolved MC–LW was 98.9 µg l
−
1
in October. MC–LR was only detected in May at a concentration of 0.5 µg l
−
1
. The cross reactivity of the antibody (MC10E7) to variants such as MC–LA MC–LW & MC–LF was low. Hence the results determined by ELISA were lower than those determined by HPLC in September and October samples due to differences in the specificity of the antibody to MC variants. Total extraceullar MCs was quantified by ELISA and ranged from 0.73 to 48.5 µg MC–LR equivalents l
−
1
, which in some cases exceeded the WHO provisional Guideline Value for MC–LR in drinking water. This study confirms that the lakes of Turkey should be monitored for toxic cyanobacteria and for MCs to avoid or reduce the potential exposure of people to these health hazards.</abstract><cop>Kidlington</cop><pub>Elsevier B.V</pub><pmid>19395066</pmid><doi>10.1016/j.scitotenv.2009.02.039</doi><tpages>9</tpages></addata></record> |
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| identifier | ISSN: 0048-9697 |
| ispartof | The Science of the total environment, 2009-06, Vol.407 (13), p.4038-4046 |
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| subjects | Animal and plant ecology Animal, plant and microbial ecology Antibodies Biological and medical sciences Biomass Chromatography, High Pressure Liquid Cyanobacteria Cyanobacteria - chemistry Cyanobacteria - isolation & purification Dissolution ELISA Enzyme-Linked Immunosorbent Assay Fresh Water - chemistry Fresh water ecosystems Freshwater Fundamental and applied biological sciences. Psychology Guidelines HPLC analysis Kovada Lake Lakes Microcystin Microcystins - analysis Microcystis Microcystis aeruginosa Phormidium Planktothrix Quantitative analysis Solubility Stations Synechococcus Synecology Turkey Water Microbiology |
| title | Analysis of dissolved microcystins in surface water samples from Kovada Lake, Turkey |
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