Knowledge-guided laboratory evolution of protein thermolability

In rare but nevertheless important cases it is of practical interest to decrease the thermostability of an enzyme, that is, to increase thermolability in a controlled manner. In the present model study, this unconventional goal has been reached by applying directed evolution to the lipase from Pseud...

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Veröffentlicht in:	Biotechnology and bioengineering 2009-04, Vol.102 (6), p.1712-1717
Hauptverfasser:	Reetz, Manfred T, Soni, Pankaj, Fernández, Layla
Format:	Artikel
Sprache:	eng
Schlagworte:	B-factors Bacteria Bacterial Proteins - genetics Bacterial Proteins - metabolism Biological and medical sciences Biotechnology Catalysis directed evolution Directed Molecular Evolution - methods Enzyme Stability - genetics enzyme thermolability Enzymes Evolution & development Fundamental and applied biological sciences. Psychology Gene Library Lipase - chemistry Lipase - genetics lipases Methods. Procedures. Technologies Models, Molecular Mutagenesis Mutation Protein engineering Proteins Pseudomonas aeruginosa Pseudomonas aeruginosa - enzymology Pseudomonas aeruginosa - genetics saturation mutagenesis Substrate Specificity - genetics Temperature
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