Cellobiose dehydrogenase production by the mycelial culture of the mushroom Termitomyces clypeatus
Termitomyces clypeatus produces cellobiose dehydrogenase (CDHtc) in cellulose medium with the highest yield (55.88 U mL −1) among all the reported fungal species. The enzyme has been isolated and purified from the culture filtrate. CDHtc was found to be a very thermolabile enzyme with the temperatur...
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Veröffentlicht in: | Process biochemistry (1991) 2008-06, Vol.43 (6), p.634-641 |
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container_title | Process biochemistry (1991) |
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creator | Saha, Tanima Ghosh, Debi Mukherjee, Srijata Bose, Shilpi Mukherjee, Mina |
description | Termitomyces clypeatus produces cellobiose dehydrogenase (CDHtc) in cellulose medium with the highest yield (55.88
U
mL
−1) among all the reported fungal species. The enzyme has been isolated and purified from the culture filtrate.
CDHtc was found to be a very thermolabile enzyme with the temperature optimum at 30
°C, while it exhibited a wide range of pH stability from pH 2.0 to 8.0. Lactose was efficiently converted to lactobionic acid in presence of the enzyme. Addition of glucose in the cellulose medium on the first day of growth induced a lag period in enzyme production but ultimately facilitated earlier CDHtc production and the yield was also comparable to that achieved in the cellulose medium. |
doi_str_mv | 10.1016/j.procbio.2008.01.025 |
format | Article |
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U
mL
−1) among all the reported fungal species. The enzyme has been isolated and purified from the culture filtrate.
CDHtc was found to be a very thermolabile enzyme with the temperature optimum at 30
°C, while it exhibited a wide range of pH stability from pH 2.0 to 8.0. Lactose was efficiently converted to lactobionic acid in presence of the enzyme. Addition of glucose in the cellulose medium on the first day of growth induced a lag period in enzyme production but ultimately facilitated earlier CDHtc production and the yield was also comparable to that achieved in the cellulose medium.</description><identifier>ISSN: 1359-5113</identifier><identifier>EISSN: 1873-3298</identifier><identifier>DOI: 10.1016/j.procbio.2008.01.025</identifier><language>eng</language><publisher>Elsevier Ltd</publisher><subject>Cellobiose dehydrogenase ; Edible fungus ; Glucose effect ; Lactobionic acid ; Termitomyces clypeatus ; Thermolabile enzyme</subject><ispartof>Process biochemistry (1991), 2008-06, Vol.43 (6), p.634-641</ispartof><rights>2008 Elsevier Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c408t-40ab1c894576af02a55b387c07bb498d1ba97e141aab63868fa013e5981c6c203</citedby><cites>FETCH-LOGICAL-c408t-40ab1c894576af02a55b387c07bb498d1ba97e141aab63868fa013e5981c6c203</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.procbio.2008.01.025$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3541,27915,27916,45986</link.rule.ids></links><search><creatorcontrib>Saha, Tanima</creatorcontrib><creatorcontrib>Ghosh, Debi</creatorcontrib><creatorcontrib>Mukherjee, Srijata</creatorcontrib><creatorcontrib>Bose, Shilpi</creatorcontrib><creatorcontrib>Mukherjee, Mina</creatorcontrib><title>Cellobiose dehydrogenase production by the mycelial culture of the mushroom Termitomyces clypeatus</title><title>Process biochemistry (1991)</title><description>Termitomyces clypeatus produces cellobiose dehydrogenase (CDHtc) in cellulose medium with the highest yield (55.88
U
mL
−1) among all the reported fungal species. The enzyme has been isolated and purified from the culture filtrate.
CDHtc was found to be a very thermolabile enzyme with the temperature optimum at 30
°C, while it exhibited a wide range of pH stability from pH 2.0 to 8.0. Lactose was efficiently converted to lactobionic acid in presence of the enzyme. Addition of glucose in the cellulose medium on the first day of growth induced a lag period in enzyme production but ultimately facilitated earlier CDHtc production and the yield was also comparable to that achieved in the cellulose medium.</description><subject>Cellobiose dehydrogenase</subject><subject>Edible fungus</subject><subject>Glucose effect</subject><subject>Lactobionic acid</subject><subject>Termitomyces clypeatus</subject><subject>Thermolabile enzyme</subject><issn>1359-5113</issn><issn>1873-3298</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><recordid>eNqFkEtLxDAUhYsoOI7-BKErd633Nn2kK5HBFwy4GdchSW-dDG0zJq3Qf2-Gup_VfXDO4d4viu4RUgQsHw_p0VmtjE0zAJ4CppAVF9EKecUSltX8MvSsqJMCkV1HN94fABgiwipSG-o6G7ye4ob2c-PsNw0yTCGzmfRo7BCrOR73FPezps7ILtZTN06OYtsu-8nvnbV9vCPXm9GedD7W3XwkOU7-NrpqZefp7r-uo6_Xl93mPdl-vn1snreJzoGPSQ5SoeZ1XlSlbCGTRaEYrzRUSuU1b1DJuiLMUUpVMl7yVgIyKmqOutQZsHX0sOSGy38m8qPojQ8Xd3IgO3nBWJbVecXOCrEuec6hDMJiEWpnvXfUiqMzvXSzQBAn9OIg_tGLE3oBKAL64HtafBTe_TXkhNeGBk2NcaRH0VhzJuEPebWRdQ</recordid><startdate>20080601</startdate><enddate>20080601</enddate><creator>Saha, Tanima</creator><creator>Ghosh, Debi</creator><creator>Mukherjee, Srijata</creator><creator>Bose, Shilpi</creator><creator>Mukherjee, Mina</creator><general>Elsevier Ltd</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>7U5</scope><scope>F28</scope><scope>L7M</scope></search><sort><creationdate>20080601</creationdate><title>Cellobiose dehydrogenase production by the mycelial culture of the mushroom Termitomyces clypeatus</title><author>Saha, Tanima ; Ghosh, Debi ; Mukherjee, Srijata ; Bose, Shilpi ; Mukherjee, Mina</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c408t-40ab1c894576af02a55b387c07bb498d1ba97e141aab63868fa013e5981c6c203</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Cellobiose dehydrogenase</topic><topic>Edible fungus</topic><topic>Glucose effect</topic><topic>Lactobionic acid</topic><topic>Termitomyces clypeatus</topic><topic>Thermolabile enzyme</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Saha, Tanima</creatorcontrib><creatorcontrib>Ghosh, Debi</creatorcontrib><creatorcontrib>Mukherjee, Srijata</creatorcontrib><creatorcontrib>Bose, Shilpi</creatorcontrib><creatorcontrib>Mukherjee, Mina</creatorcontrib><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Advanced Technologies Database with Aerospace</collection><jtitle>Process biochemistry (1991)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Saha, Tanima</au><au>Ghosh, Debi</au><au>Mukherjee, Srijata</au><au>Bose, Shilpi</au><au>Mukherjee, Mina</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cellobiose dehydrogenase production by the mycelial culture of the mushroom Termitomyces clypeatus</atitle><jtitle>Process biochemistry (1991)</jtitle><date>2008-06-01</date><risdate>2008</risdate><volume>43</volume><issue>6</issue><spage>634</spage><epage>641</epage><pages>634-641</pages><issn>1359-5113</issn><eissn>1873-3298</eissn><abstract>Termitomyces clypeatus produces cellobiose dehydrogenase (CDHtc) in cellulose medium with the highest yield (55.88
U
mL
−1) among all the reported fungal species. The enzyme has been isolated and purified from the culture filtrate.
CDHtc was found to be a very thermolabile enzyme with the temperature optimum at 30
°C, while it exhibited a wide range of pH stability from pH 2.0 to 8.0. Lactose was efficiently converted to lactobionic acid in presence of the enzyme. Addition of glucose in the cellulose medium on the first day of growth induced a lag period in enzyme production but ultimately facilitated earlier CDHtc production and the yield was also comparable to that achieved in the cellulose medium.</abstract><pub>Elsevier Ltd</pub><doi>10.1016/j.procbio.2008.01.025</doi><tpages>8</tpages></addata></record> |
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ispartof | Process biochemistry (1991), 2008-06, Vol.43 (6), p.634-641 |
issn | 1359-5113 1873-3298 |
language | eng |
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source | ScienceDirect Journals (5 years ago - present) |
subjects | Cellobiose dehydrogenase Edible fungus Glucose effect Lactobionic acid Termitomyces clypeatus Thermolabile enzyme |
title | Cellobiose dehydrogenase production by the mycelial culture of the mushroom Termitomyces clypeatus |
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