Silica-overcoated substrates for detection of proteins by surface-enhanced Raman spectroscopy
Ag film over nanosphere (AgFON) substrates for surface‐enhanced Raman spectroscopy (SERS) are shown to be ineffective for the detection of proteins in phosphate buffer solution (PBS) because of the decomposition of the substrate resulting in a total loss of SERS activity. However, modification of th...
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| Veröffentlicht in: | Journal of Raman spectroscopy 2008-05, Vol.39 (5), p.673-678 |
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| container_end_page | 678 |
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| container_title | Journal of Raman spectroscopy |
| container_volume | 39 |
| creator | Scholes, F. H. Bendavid, A. Glenn, F. L. Critchley, M. Davis, T. J. Sexton, B. A. |
| description | Ag film over nanosphere (AgFON) substrates for surface‐enhanced Raman spectroscopy (SERS) are shown to be ineffective for the detection of proteins in phosphate buffer solution (PBS) because of the decomposition of the substrate resulting in a total loss of SERS activity. However, modification of these substrates with SiO2 overlayers overcomes this problem. The SiO2 overlayers are produced by filtered arc deposition (FAD) and are characterised by atomic force microscopy (AFM). Their porosity is examined using Raman spectroscopy and the detection of cytochrome c and bovine serum albumin in PBS is successfully demonstrated. These findings show promise for the detection of proteins in biologically relevant conditions using Ag‐based SERS substrates. Copyright © 2008 John Wiley & Sons, Ltd. |
| doi_str_mv | 10.1002/jrs.1914 |
| format | Article |
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H. ; Bendavid, A. ; Glenn, F. L. ; Critchley, M. ; Davis, T. J. ; Sexton, B. A.</creator><creatorcontrib>Scholes, F. H. ; Bendavid, A. ; Glenn, F. L. ; Critchley, M. ; Davis, T. J. ; Sexton, B. A.</creatorcontrib><description>Ag film over nanosphere (AgFON) substrates for surface‐enhanced Raman spectroscopy (SERS) are shown to be ineffective for the detection of proteins in phosphate buffer solution (PBS) because of the decomposition of the substrate resulting in a total loss of SERS activity. However, modification of these substrates with SiO2 overlayers overcomes this problem. The SiO2 overlayers are produced by filtered arc deposition (FAD) and are characterised by atomic force microscopy (AFM). Their porosity is examined using Raman spectroscopy and the detection of cytochrome c and bovine serum albumin in PBS is successfully demonstrated. These findings show promise for the detection of proteins in biologically relevant conditions using Ag‐based SERS substrates. 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Their porosity is examined using Raman spectroscopy and the detection of cytochrome c and bovine serum albumin in PBS is successfully demonstrated. These findings show promise for the detection of proteins in biologically relevant conditions using Ag‐based SERS substrates. Copyright © 2008 John Wiley & Sons, Ltd.</description><subject>Atomic force microscopy</subject><subject>Nanocomposites</subject><subject>Nanomaterials</subject><subject>Nanostructure</subject><subject>Proteins</subject><subject>Raman spectroscopy</subject><subject>silica</subject><subject>Silicon dioxide</subject><subject>Silver</subject><subject>surface-enhanced Raman spectroscopy (SERS)</subject><issn>0377-0486</issn><issn>1097-4555</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><recordid>eNp90N9LwzAQB_AgCs4p-Cf0SXzpzDVJkz6K6FSGwvz1JCFLr1jtmpl0av97MyaKD_qUg3zuuPsSsg90BJRmR88-jKAAvkEGQAuZciHEJhlQJmVKucq3yU4Iz5TSoshhQB5v6qa2JnVv6K0zHZZJWM5C52MZksr5pMQObVe7NnFVsvCuw7oNyayPzlfGYortk2ltbJyauWmTsIjcu2Ddot8lW5VpAu59vUNyd3Z6e3KeTq7HFyfHk9RyxngquChLxXIKyISSKstQcm450JlVIBkYqwoGsixlCTwWRlDgGP8zUHku2ZAcrOfG_V6XGDo9r4PFpjEtumXQjIHIqFrBw38h5BI4LQQvfqiNxwSPlV74em58r4HqVdQ6Rq1XUUearul73WD_p9OX05vfvg4dfnx74190PEYK_XA11g9yMlX5_bkG9gmRs44F</recordid><startdate>200805</startdate><enddate>200805</enddate><creator>Scholes, F. H.</creator><creator>Bendavid, A.</creator><creator>Glenn, F. L.</creator><creator>Critchley, M.</creator><creator>Davis, T. J.</creator><creator>Sexton, B. A.</creator><general>John Wiley & Sons, Ltd</general><scope>BSCLL</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope><scope>L7M</scope></search><sort><creationdate>200805</creationdate><title>Silica-overcoated substrates for detection of proteins by surface-enhanced Raman spectroscopy</title><author>Scholes, F. H. ; Bendavid, A. ; Glenn, F. L. ; Critchley, M. ; Davis, T. J. ; Sexton, B. 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Raman Spectrosc</addtitle><date>2008-05</date><risdate>2008</risdate><volume>39</volume><issue>5</issue><spage>673</spage><epage>678</epage><pages>673-678</pages><issn>0377-0486</issn><eissn>1097-4555</eissn><abstract>Ag film over nanosphere (AgFON) substrates for surface‐enhanced Raman spectroscopy (SERS) are shown to be ineffective for the detection of proteins in phosphate buffer solution (PBS) because of the decomposition of the substrate resulting in a total loss of SERS activity. However, modification of these substrates with SiO2 overlayers overcomes this problem. The SiO2 overlayers are produced by filtered arc deposition (FAD) and are characterised by atomic force microscopy (AFM). Their porosity is examined using Raman spectroscopy and the detection of cytochrome c and bovine serum albumin in PBS is successfully demonstrated. These findings show promise for the detection of proteins in biologically relevant conditions using Ag‐based SERS substrates. Copyright © 2008 John Wiley & Sons, Ltd.</abstract><cop>Chichester, UK</cop><pub>John Wiley & Sons, Ltd</pub><doi>10.1002/jrs.1914</doi><tpages>6</tpages></addata></record> |
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| ispartof | Journal of Raman spectroscopy, 2008-05, Vol.39 (5), p.673-678 |
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| subjects | Atomic force microscopy Nanocomposites Nanomaterials Nanostructure Proteins Raman spectroscopy silica Silicon dioxide Silver surface-enhanced Raman spectroscopy (SERS) |
| title | Silica-overcoated substrates for detection of proteins by surface-enhanced Raman spectroscopy |
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