Layer-by-layer immobilization of horseradish peroxidase on a gold electrode modified with colloidal gold nanoparticles
. An amperometric biosensor, based on layer-by-layer self-assembly of colloidal gold nanoparticles, cysteine and horseradish peroxidase on Nafion modified electrode surface by electrostatic adsorption, has been used for the determination of hydrogen peroxide. The electrochemical behavior of the mult...
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| Veröffentlicht in: | Mikrochimica acta (1966) 2008-04, Vol.161 (1-2), p.241-248 |
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| container_title | Mikrochimica acta (1966) |
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| creator | Liu, Yan Geng, Tongmou Gao, Jie |
| description | .
An amperometric biosensor, based on layer-by-layer self-assembly of colloidal gold nanoparticles, cysteine and horseradish peroxidase on Nafion modified electrode surface by electrostatic adsorption, has been used for the determination of hydrogen peroxide. The electrochemical behavior of the multilayer film was studied by cyclic voltammetry, linear sweep voltammetry and chronoamperometry. The step layer-by-layer adsorption interface morphology was further characterized by means of electrochemical impedance spectroscopy and cyclic voltammetry. The performance and factors influencing the resulted biosensor were studied in detail. The sensor displayed an excellent electrocatalytic response to the reduction of H
2
O
2
without the aid of an electron transfer mediator. Linear response to H
2
O
2
was obtained for the concentration range from 1.6 µM to 2.4 mM under optimized conditions. The detection limit of the biosensor was 0.5 µM (S/N = 3), and the sensor achieved 95% of the steady-state current within 10 s. The sensor exhibited high sensitivity, selectivity and stability. |
| doi_str_mv | 10.1007/s00604-007-0779-y |
| format | Article |
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An amperometric biosensor, based on layer-by-layer self-assembly of colloidal gold nanoparticles, cysteine and horseradish peroxidase on Nafion modified electrode surface by electrostatic adsorption, has been used for the determination of hydrogen peroxide. The electrochemical behavior of the multilayer film was studied by cyclic voltammetry, linear sweep voltammetry and chronoamperometry. The step layer-by-layer adsorption interface morphology was further characterized by means of electrochemical impedance spectroscopy and cyclic voltammetry. The performance and factors influencing the resulted biosensor were studied in detail. The sensor displayed an excellent electrocatalytic response to the reduction of H
2
O
2
without the aid of an electron transfer mediator. Linear response to H
2
O
2
was obtained for the concentration range from 1.6 µM to 2.4 mM under optimized conditions. The detection limit of the biosensor was 0.5 µM (S/N = 3), and the sensor achieved 95% of the steady-state current within 10 s. The sensor exhibited high sensitivity, selectivity and stability.</description><identifier>ISSN: 0026-3672</identifier><identifier>EISSN: 1436-5073</identifier><identifier>DOI: 10.1007/s00604-007-0779-y</identifier><identifier>CODEN: MIACAQ</identifier><language>eng</language><publisher>Vienna: Springer-Verlag</publisher><subject>Analytical Chemistry ; Biological and medical sciences ; Biosensors ; Biotechnology ; Characterization and Evaluation of Materials ; Chemistry ; Chemistry and Materials Science ; Electrochemical methods ; Exact sciences and technology ; Fundamental and applied biological sciences. Psychology ; General, instrumentation ; Methods. Procedures. Technologies ; Microengineering ; Nanochemistry ; Nanotechnology ; Original Paper ; Various methods and equipments</subject><ispartof>Mikrochimica acta (1966), 2008-04, Vol.161 (1-2), p.241-248</ispartof><rights>Springer-Verlag 2007</rights><rights>2008 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c349t-abc6e6c8ec39711c7b023e53e3af89583b2b2cf8a4d5c6fff893489317fe966c3</citedby><cites>FETCH-LOGICAL-c349t-abc6e6c8ec39711c7b023e53e3af89583b2b2cf8a4d5c6fff893489317fe966c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00604-007-0779-y$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00604-007-0779-y$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20597888$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Liu, Yan</creatorcontrib><creatorcontrib>Geng, Tongmou</creatorcontrib><creatorcontrib>Gao, Jie</creatorcontrib><title>Layer-by-layer immobilization of horseradish peroxidase on a gold electrode modified with colloidal gold nanoparticles</title><title>Mikrochimica acta (1966)</title><addtitle>Microchim Acta</addtitle><description>.
An amperometric biosensor, based on layer-by-layer self-assembly of colloidal gold nanoparticles, cysteine and horseradish peroxidase on Nafion modified electrode surface by electrostatic adsorption, has been used for the determination of hydrogen peroxide. The electrochemical behavior of the multilayer film was studied by cyclic voltammetry, linear sweep voltammetry and chronoamperometry. The step layer-by-layer adsorption interface morphology was further characterized by means of electrochemical impedance spectroscopy and cyclic voltammetry. The performance and factors influencing the resulted biosensor were studied in detail. The sensor displayed an excellent electrocatalytic response to the reduction of H
2
O
2
without the aid of an electron transfer mediator. Linear response to H
2
O
2
was obtained for the concentration range from 1.6 µM to 2.4 mM under optimized conditions. The detection limit of the biosensor was 0.5 µM (S/N = 3), and the sensor achieved 95% of the steady-state current within 10 s. The sensor exhibited high sensitivity, selectivity and stability.</description><subject>Analytical Chemistry</subject><subject>Biological and medical sciences</subject><subject>Biosensors</subject><subject>Biotechnology</subject><subject>Characterization and Evaluation of Materials</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Electrochemical methods</subject><subject>Exact sciences and technology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General, instrumentation</subject><subject>Methods. Procedures. Technologies</subject><subject>Microengineering</subject><subject>Nanochemistry</subject><subject>Nanotechnology</subject><subject>Original Paper</subject><subject>Various methods and equipments</subject><issn>0026-3672</issn><issn>1436-5073</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><recordid>eNp9kE1PxCAQhonRxPXjB3jjojcUSgvt0Ri_kk286JlQOuyyoWWFrlp_vWxqPHqYzJuZZ95kXoQuGL1mlMqbRKmgJcmSUCkbMh2gBSu5IBWV_BAtKC0E4UIWx-gkpQ2lTIqiXKCPpZ4gknYifi-w6_vQOu--9ejCgIPF6xATRN25tMZbiOHLdToBzkuNV8F3GDyYMYYOcB86Zx10-NONa2yC9yHDfsYGPYStjqMzHtIZOrLaJzj_7afo7eH-9e6JLF8en-9ul8TwshmJbo0AYWowvJGMGdnSgkPFgWtbN1XN26ItjK112VVGWJuHvMzFpIVGCMNP0dXsu43hfQdpVL1LBrzXA4RdUpznxGomM8hm0MSQUgSrttH1Ok6KUbVPWM0Jq73cJ6ymfHP5a66T0d5GPRiX_g4LWjWyruvMFTOX8mpYQVSbsItD_vsf8x8fno8L</recordid><startdate>20080401</startdate><enddate>20080401</enddate><creator>Liu, Yan</creator><creator>Geng, Tongmou</creator><creator>Gao, Jie</creator><general>Springer-Verlag</general><general>Springer</general><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope><scope>L7M</scope></search><sort><creationdate>20080401</creationdate><title>Layer-by-layer immobilization of horseradish peroxidase on a gold electrode modified with colloidal gold nanoparticles</title><author>Liu, Yan ; Geng, Tongmou ; Gao, Jie</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c349t-abc6e6c8ec39711c7b023e53e3af89583b2b2cf8a4d5c6fff893489317fe966c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Analytical Chemistry</topic><topic>Biological and medical sciences</topic><topic>Biosensors</topic><topic>Biotechnology</topic><topic>Characterization and Evaluation of Materials</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Electrochemical methods</topic><topic>Exact sciences and technology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General, instrumentation</topic><topic>Methods. Procedures. Technologies</topic><topic>Microengineering</topic><topic>Nanochemistry</topic><topic>Nanotechnology</topic><topic>Original Paper</topic><topic>Various methods and equipments</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liu, Yan</creatorcontrib><creatorcontrib>Geng, Tongmou</creatorcontrib><creatorcontrib>Gao, Jie</creatorcontrib><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><jtitle>Mikrochimica acta (1966)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liu, Yan</au><au>Geng, Tongmou</au><au>Gao, Jie</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Layer-by-layer immobilization of horseradish peroxidase on a gold electrode modified with colloidal gold nanoparticles</atitle><jtitle>Mikrochimica acta (1966)</jtitle><stitle>Microchim Acta</stitle><date>2008-04-01</date><risdate>2008</risdate><volume>161</volume><issue>1-2</issue><spage>241</spage><epage>248</epage><pages>241-248</pages><issn>0026-3672</issn><eissn>1436-5073</eissn><coden>MIACAQ</coden><abstract>.
An amperometric biosensor, based on layer-by-layer self-assembly of colloidal gold nanoparticles, cysteine and horseradish peroxidase on Nafion modified electrode surface by electrostatic adsorption, has been used for the determination of hydrogen peroxide. The electrochemical behavior of the multilayer film was studied by cyclic voltammetry, linear sweep voltammetry and chronoamperometry. The step layer-by-layer adsorption interface morphology was further characterized by means of electrochemical impedance spectroscopy and cyclic voltammetry. The performance and factors influencing the resulted biosensor were studied in detail. The sensor displayed an excellent electrocatalytic response to the reduction of H
2
O
2
without the aid of an electron transfer mediator. Linear response to H
2
O
2
was obtained for the concentration range from 1.6 µM to 2.4 mM under optimized conditions. The detection limit of the biosensor was 0.5 µM (S/N = 3), and the sensor achieved 95% of the steady-state current within 10 s. The sensor exhibited high sensitivity, selectivity and stability.</abstract><cop>Vienna</cop><pub>Springer-Verlag</pub><doi>10.1007/s00604-007-0779-y</doi><tpages>8</tpages></addata></record> |
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| subjects | Analytical Chemistry Biological and medical sciences Biosensors Biotechnology Characterization and Evaluation of Materials Chemistry Chemistry and Materials Science Electrochemical methods Exact sciences and technology Fundamental and applied biological sciences. Psychology General, instrumentation Methods. Procedures. Technologies Microengineering Nanochemistry Nanotechnology Original Paper Various methods and equipments |
| title | Layer-by-layer immobilization of horseradish peroxidase on a gold electrode modified with colloidal gold nanoparticles |
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