Growth, viability, adhesion potential, and fibronectin expression in fibroblasts cultured on zirconia or feldspatic ceramics in vitro
Zirconia, a biomaterial widely used in dentistry, has recently attracted much attention for its mechanical strength and toughness. Previously, its lack of mutagenic and carcinogenic power was reported. We describe here other essential aspects to be taken into account to define in vitro the biocompat...
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creator | Raffaelli, Luca Iommetti, Pierfrancesco Rossi Piccioni, Elisabetta Toesca, Amelia Serini, Simona Resci, Federica Missori, Mauro De Spirito, Marco Manicone, Paolo F. Calviello, Gabriella |
description | Zirconia, a biomaterial widely used in dentistry, has recently attracted much attention for its mechanical strength and toughness. Previously, its lack of mutagenic and carcinogenic power was reported. We describe here other essential aspects to be taken into account to define in vitro the biocompatibility of a material: the growth rate, viability, and adhesion capacity of normal stabilized cells growing on it. To this aim, immortalized RAT‐1 fibroblasts, growing either on zirconia and on feldspatic (FE) ceramics were compared. In particular, the level of expression and the intra‐ and extra‐cellular organization of fibronectin, a glycoprotein involved in cellular adhesion and migration during tissue repair, was analyzed. Fibroblasts cultured on zirconia showed a higher growth rate, and underwent necrosis at lower levels than cells on FE ceramic, whereas either materials did not stimulate apoptosis. Adhesion capacity of fibroblasts was evaluated measuring adherent cell nucleic acids with the fluorimetric CyQuant® assay, and it was found significantly higher in cells cultured on zirconia than on FE ceramic. This finding may be explained by the higher and more precocious expression of the adhesion protein fibronectin observed by indirect immunofluorescence in fibroblasts on zirconia. Overall, the results suggest that zirconia, exerting low cytotoxicity and strongly inducing adhesion capacity, increases cellular growth rate of fibroblasts. All these features suggest that zirconia could represent a more suitable biomaterial than FE ceramic for prosthesis in dentistry. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res 2008 |
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Previously, its lack of mutagenic and carcinogenic power was reported. We describe here other essential aspects to be taken into account to define in vitro the biocompatibility of a material: the growth rate, viability, and adhesion capacity of normal stabilized cells growing on it. To this aim, immortalized RAT‐1 fibroblasts, growing either on zirconia and on feldspatic (FE) ceramics were compared. In particular, the level of expression and the intra‐ and extra‐cellular organization of fibronectin, a glycoprotein involved in cellular adhesion and migration during tissue repair, was analyzed. Fibroblasts cultured on zirconia showed a higher growth rate, and underwent necrosis at lower levels than cells on FE ceramic, whereas either materials did not stimulate apoptosis. Adhesion capacity of fibroblasts was evaluated measuring adherent cell nucleic acids with the fluorimetric CyQuant® assay, and it was found significantly higher in cells cultured on zirconia than on FE ceramic. This finding may be explained by the higher and more precocious expression of the adhesion protein fibronectin observed by indirect immunofluorescence in fibroblasts on zirconia. Overall, the results suggest that zirconia, exerting low cytotoxicity and strongly inducing adhesion capacity, increases cellular growth rate of fibroblasts. All these features suggest that zirconia could represent a more suitable biomaterial than FE ceramic for prosthesis in dentistry. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res 2008</description><identifier>ISSN: 1549-3296</identifier><identifier>EISSN: 1552-4965</identifier><identifier>DOI: 10.1002/jbm.a.31693</identifier><identifier>PMID: 18067158</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Aluminum Silicates - pharmacology ; Animals ; Apoptosis - drug effects ; Cell Adhesion - drug effects ; Cell Line ; Cell Proliferation - drug effects ; Cell Survival - drug effects ; Cells, Cultured ; cellular adhesion ; cellular growth rate ; Ceramics - pharmacology ; Fibroblasts - cytology ; Fibroblasts - drug effects ; Fibroblasts - ultrastructure ; fibronectin ; Fibronectins - metabolism ; fixed partial dentures ; Fluorescent Antibody Technique ; Polystyrenes - pharmacology ; Potassium Compounds - pharmacology ; Rats ; zirconia ; Zirconium - pharmacology</subject><ispartof>Journal of biomedical materials research. 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Part A</title><addtitle>J. Biomed. Mater. Res</addtitle><description>Zirconia, a biomaterial widely used in dentistry, has recently attracted much attention for its mechanical strength and toughness. Previously, its lack of mutagenic and carcinogenic power was reported. We describe here other essential aspects to be taken into account to define in vitro the biocompatibility of a material: the growth rate, viability, and adhesion capacity of normal stabilized cells growing on it. To this aim, immortalized RAT‐1 fibroblasts, growing either on zirconia and on feldspatic (FE) ceramics were compared. In particular, the level of expression and the intra‐ and extra‐cellular organization of fibronectin, a glycoprotein involved in cellular adhesion and migration during tissue repair, was analyzed. Fibroblasts cultured on zirconia showed a higher growth rate, and underwent necrosis at lower levels than cells on FE ceramic, whereas either materials did not stimulate apoptosis. Adhesion capacity of fibroblasts was evaluated measuring adherent cell nucleic acids with the fluorimetric CyQuant® assay, and it was found significantly higher in cells cultured on zirconia than on FE ceramic. This finding may be explained by the higher and more precocious expression of the adhesion protein fibronectin observed by indirect immunofluorescence in fibroblasts on zirconia. Overall, the results suggest that zirconia, exerting low cytotoxicity and strongly inducing adhesion capacity, increases cellular growth rate of fibroblasts. All these features suggest that zirconia could represent a more suitable biomaterial than FE ceramic for prosthesis in dentistry. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res 2008</description><subject>Aluminum Silicates - pharmacology</subject><subject>Animals</subject><subject>Apoptosis - drug effects</subject><subject>Cell Adhesion - drug effects</subject><subject>Cell Line</subject><subject>Cell Proliferation - drug effects</subject><subject>Cell Survival - drug effects</subject><subject>Cells, Cultured</subject><subject>cellular adhesion</subject><subject>cellular growth rate</subject><subject>Ceramics - pharmacology</subject><subject>Fibroblasts - cytology</subject><subject>Fibroblasts - drug effects</subject><subject>Fibroblasts - ultrastructure</subject><subject>fibronectin</subject><subject>Fibronectins - metabolism</subject><subject>fixed partial dentures</subject><subject>Fluorescent Antibody Technique</subject><subject>Polystyrenes - pharmacology</subject><subject>Potassium Compounds - pharmacology</subject><subject>Rats</subject><subject>zirconia</subject><subject>Zirconium - pharmacology</subject><issn>1549-3296</issn><issn>1552-4965</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kLtv1TAchS0Eoi8mduSJheZiO7GdjLSCy6NQhiKkLpbj_Ky6JHGwnba3e_9vfB-FjcmP850zfAi9pGRBCWFvr9thoRclFU35BO1TzllRNYI_Xd-rpihZI_bQQYzXGRaEs-doj9ZESMrrffSwDP42XR3jG6db17u0Osa6u4Lo_Ignn2BMTvf5b-ywdW3wI5jkRgx3U4C4ofJrk7S9jiliM_dpDtDhHN27YPzoNPYBW-i7OOnkDDYQ9OBMXFdvXAr-CD2zuo_wYnceoh8f3l-cfizOzpefTt-dFaYSVVnwWuuWyK5rSMWhtppKwnhLWs6IlgSYpMIA44YQ4NLWNocNr6hlUNqu5uUher3dnYL_PUNManDRQN_rEfwcVXZFRCObDL7Zgib4GANYNQU36LBSlKi1dZWtK6021jP9ajc7twN0_9id5gzQLXDrelj9b0t9Pvn6OFpsOy4muPvb0eGXErKUXP38tlRZAL-8_P5FXZR_ADXfnwg</recordid><startdate>20080915</startdate><enddate>20080915</enddate><creator>Raffaelli, Luca</creator><creator>Iommetti, Pierfrancesco Rossi</creator><creator>Piccioni, Elisabetta</creator><creator>Toesca, Amelia</creator><creator>Serini, Simona</creator><creator>Resci, Federica</creator><creator>Missori, Mauro</creator><creator>De Spirito, Marco</creator><creator>Manicone, Paolo F.</creator><creator>Calviello, Gabriella</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>7TB</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>F28</scope><scope>FR3</scope><scope>JG9</scope><scope>L7M</scope></search><sort><creationdate>20080915</creationdate><title>Growth, viability, adhesion potential, and fibronectin expression in fibroblasts cultured on zirconia or feldspatic ceramics in vitro</title><author>Raffaelli, Luca ; Iommetti, Pierfrancesco Rossi ; Piccioni, Elisabetta ; Toesca, Amelia ; Serini, Simona ; Resci, Federica ; Missori, Mauro ; De Spirito, Marco ; Manicone, Paolo F. ; Calviello, Gabriella</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4643-58aab07dd9045e8fa17025b0b520a70e2716ce25c00e57f8f25b9541f2e3fd853</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Aluminum Silicates - pharmacology</topic><topic>Animals</topic><topic>Apoptosis - drug effects</topic><topic>Cell Adhesion - drug effects</topic><topic>Cell Line</topic><topic>Cell Proliferation - drug effects</topic><topic>Cell Survival - drug effects</topic><topic>Cells, Cultured</topic><topic>cellular adhesion</topic><topic>cellular growth rate</topic><topic>Ceramics - pharmacology</topic><topic>Fibroblasts - cytology</topic><topic>Fibroblasts - drug effects</topic><topic>Fibroblasts - ultrastructure</topic><topic>fibronectin</topic><topic>Fibronectins - metabolism</topic><topic>fixed partial dentures</topic><topic>Fluorescent Antibody Technique</topic><topic>Polystyrenes - pharmacology</topic><topic>Potassium Compounds - pharmacology</topic><topic>Rats</topic><topic>zirconia</topic><topic>Zirconium - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Raffaelli, Luca</creatorcontrib><creatorcontrib>Iommetti, Pierfrancesco Rossi</creatorcontrib><creatorcontrib>Piccioni, Elisabetta</creatorcontrib><creatorcontrib>Toesca, Amelia</creatorcontrib><creatorcontrib>Serini, Simona</creatorcontrib><creatorcontrib>Resci, Federica</creatorcontrib><creatorcontrib>Missori, Mauro</creatorcontrib><creatorcontrib>De Spirito, Marco</creatorcontrib><creatorcontrib>Manicone, Paolo F.</creatorcontrib><creatorcontrib>Calviello, Gabriella</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><jtitle>Journal of biomedical materials research. Part A</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Raffaelli, Luca</au><au>Iommetti, Pierfrancesco Rossi</au><au>Piccioni, Elisabetta</au><au>Toesca, Amelia</au><au>Serini, Simona</au><au>Resci, Federica</au><au>Missori, Mauro</au><au>De Spirito, Marco</au><au>Manicone, Paolo F.</au><au>Calviello, Gabriella</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Growth, viability, adhesion potential, and fibronectin expression in fibroblasts cultured on zirconia or feldspatic ceramics in vitro</atitle><jtitle>Journal of biomedical materials research. Part A</jtitle><addtitle>J. Biomed. Mater. Res</addtitle><date>2008-09-15</date><risdate>2008</risdate><volume>86A</volume><issue>4</issue><spage>959</spage><epage>968</epage><pages>959-968</pages><issn>1549-3296</issn><eissn>1552-4965</eissn><abstract>Zirconia, a biomaterial widely used in dentistry, has recently attracted much attention for its mechanical strength and toughness. Previously, its lack of mutagenic and carcinogenic power was reported. We describe here other essential aspects to be taken into account to define in vitro the biocompatibility of a material: the growth rate, viability, and adhesion capacity of normal stabilized cells growing on it. To this aim, immortalized RAT‐1 fibroblasts, growing either on zirconia and on feldspatic (FE) ceramics were compared. In particular, the level of expression and the intra‐ and extra‐cellular organization of fibronectin, a glycoprotein involved in cellular adhesion and migration during tissue repair, was analyzed. Fibroblasts cultured on zirconia showed a higher growth rate, and underwent necrosis at lower levels than cells on FE ceramic, whereas either materials did not stimulate apoptosis. Adhesion capacity of fibroblasts was evaluated measuring adherent cell nucleic acids with the fluorimetric CyQuant® assay, and it was found significantly higher in cells cultured on zirconia than on FE ceramic. This finding may be explained by the higher and more precocious expression of the adhesion protein fibronectin observed by indirect immunofluorescence in fibroblasts on zirconia. Overall, the results suggest that zirconia, exerting low cytotoxicity and strongly inducing adhesion capacity, increases cellular growth rate of fibroblasts. All these features suggest that zirconia could represent a more suitable biomaterial than FE ceramic for prosthesis in dentistry. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res 2008</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>18067158</pmid><doi>10.1002/jbm.a.31693</doi><tpages>10</tpages></addata></record> |
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subjects | Aluminum Silicates - pharmacology Animals Apoptosis - drug effects Cell Adhesion - drug effects Cell Line Cell Proliferation - drug effects Cell Survival - drug effects Cells, Cultured cellular adhesion cellular growth rate Ceramics - pharmacology Fibroblasts - cytology Fibroblasts - drug effects Fibroblasts - ultrastructure fibronectin Fibronectins - metabolism fixed partial dentures Fluorescent Antibody Technique Polystyrenes - pharmacology Potassium Compounds - pharmacology Rats zirconia Zirconium - pharmacology |
title | Growth, viability, adhesion potential, and fibronectin expression in fibroblasts cultured on zirconia or feldspatic ceramics in vitro |
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