Viability of heterotrophic bacteria in the Bay of Marseilles
Marine microorganism activities are commonly assessed by bulk methods and assigned to the total cell count. The presence in significant amounts of ghost, dead, and damaged cells makes such as assignment a non-correct one. A Nucleic Acid Double Staining protocol (NADS) of fresh water bacteria (Barbes...
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| Veröffentlicht in: | Comptes rendus. Biologies 2003-08, Vol.326 (8), p.739-750 |
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| container_title | Comptes rendus. Biologies |
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| creator | Grégori, Gérald Denis, Michel Lefèvre, Dominique Romano, Jean-Claude |
| description | Marine microorganism activities are commonly assessed by bulk methods and assigned to the total cell count. The presence in significant amounts of ghost, dead, and damaged cells makes such as assignment a non-correct one. A Nucleic Acid Double Staining protocol (NADS) of fresh water bacteria (Barbesti et al., Cytometry 40 (2000) 214-218) has been adapted to resolve viable, damaged and dead cells in marine environments (Grégori et al., Appl. Environ. Microbiol. 67 (2001) 4662-4670). The present reports the first in situ application of this approach, conducted in the Bay of Marseilles in winter and spring periods at two sites with contrasted features. |
| doi_str_mv | 10.1016/j.crvi.2003.07.001 |
| format | Article |
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| ispartof | Comptes rendus. Biologies, 2003-08, Vol.326 (8), p.739-750 |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete; EZB Electronic Journals Library |
| subjects | Bacteria - isolation & purification Bacterial Physiological Phenomena Bacteriological Techniques Cell Membrane Permeability DNA, Bacterial - analysis Flow Cytometry - methods Fluorescence Resonance Energy Transfer Marine Biology - methods Mediterranean Sea Seasons Staining and Labeling - methods |
| title | Viability of heterotrophic bacteria in the Bay of Marseilles |
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