Gonadotropin-releasing hormone II and its receptor regulate motility, morphology, and kinematics of porcine spermatozoa in vitro
[Display omitted] •In the boar reproductive tract, GnRH-II and GnRHR-II are most abundant within testes.•GnRHR-II immunolocalizes to elongating spermatids and the connecting piece of sperm.•Seminal plasma of mature boars contains GnRH-II.•GnRH-II analogues affect sperm motility, morphology and kinem...
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| Veröffentlicht in: | General and comparative endocrinology 2025-01, Vol.361, p.114653, Article 114653 |
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| creator | Desaulniers, A.T. Ross, C.E. Cederberg, R.A. Lovercamp, K.W. Lents, C.A. White, B.R. |
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•In the boar reproductive tract, GnRH-II and GnRHR-II are most abundant within testes.•GnRHR-II immunolocalizes to elongating spermatids and the connecting piece of sperm.•Seminal plasma of mature boars contains GnRH-II.•GnRH-II analogues affect sperm motility, morphology and kinematics in extended semen.
The second form of gonadotropin-releasing hormone (GnRH-II) and its receptor (GnRHR-II) are abundantly produced within the porcine testis and immunolocalize within the seminiferous tubules, suggesting a role in spermatogenesis and/or sperm function. The objective of this study was to quantify GnRH-II and GnRHR-II abundance within boar reproductive tract tissues and examine their role in porcine sperm function. Immunoblotting revealed GnRHR-II abundance was 12-fold greater (P < 0.0001) within the testis compared with other reproductive organs. Within seminiferous tubules, GnRHR-II prominently immunolocalized to elongating spermatids. In ejaculated spermatozoa, GnRHR-II immunolocalized to the connecting piece. GnRH-II was also detected in seminal plasma, likely originating from the testis as GnRH-II concentrations were greatest in testicular homogenates (P < 0.0001) compared with other reproductive tissues. To assess the effects of GnRH-II/GnRHR-II on sperm function, extended semen samples were treated with GnRHR-II analogues and evaluated via computer-assisted sperm analysis (CASA). In Experiment 1, semen treatment with increasing concentrations of GnRHR-II agonist (D-ala6 GnRH-II) revealed that two concentrations (0.1 and 100 µM) tended to decrease the percentage of bent sperm tails versus vehicle-treated semen (P < 0.10). In Experiment 2, semen treatment with increasing concentrations of GnRHR antagonist (SB-75/Cetrorelix) indicated that only 10 µM SB-75 impaired CASA metrics compared with vehicle-treated samples (P < 0.05). In Experiment 3, semen treatment with both 100 µM D-ala6 GnRH-II and 10 µM SB-75 partially rescued sperm motility and morphology measures. These data suggest that GnRH-II and its receptor regulate porcine sperm function in an autocrine/paracrine manner. |
| doi_str_mv | 10.1016/j.ygcen.2024.114653 |
| format | Article |
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•In the boar reproductive tract, GnRH-II and GnRHR-II are most abundant within testes.•GnRHR-II immunolocalizes to elongating spermatids and the connecting piece of sperm.•Seminal plasma of mature boars contains GnRH-II.•GnRH-II analogues affect sperm motility, morphology and kinematics in extended semen.
The second form of gonadotropin-releasing hormone (GnRH-II) and its receptor (GnRHR-II) are abundantly produced within the porcine testis and immunolocalize within the seminiferous tubules, suggesting a role in spermatogenesis and/or sperm function. The objective of this study was to quantify GnRH-II and GnRHR-II abundance within boar reproductive tract tissues and examine their role in porcine sperm function. Immunoblotting revealed GnRHR-II abundance was 12-fold greater (P < 0.0001) within the testis compared with other reproductive organs. Within seminiferous tubules, GnRHR-II prominently immunolocalized to elongating spermatids. In ejaculated spermatozoa, GnRHR-II immunolocalized to the connecting piece. GnRH-II was also detected in seminal plasma, likely originating from the testis as GnRH-II concentrations were greatest in testicular homogenates (P < 0.0001) compared with other reproductive tissues. To assess the effects of GnRH-II/GnRHR-II on sperm function, extended semen samples were treated with GnRHR-II analogues and evaluated via computer-assisted sperm analysis (CASA). In Experiment 1, semen treatment with increasing concentrations of GnRHR-II agonist (D-ala6 GnRH-II) revealed that two concentrations (0.1 and 100 µM) tended to decrease the percentage of bent sperm tails versus vehicle-treated semen (P < 0.10). In Experiment 2, semen treatment with increasing concentrations of GnRHR antagonist (SB-75/Cetrorelix) indicated that only 10 µM SB-75 impaired CASA metrics compared with vehicle-treated samples (P < 0.05). In Experiment 3, semen treatment with both 100 µM D-ala6 GnRH-II and 10 µM SB-75 partially rescued sperm motility and morphology measures. These data suggest that GnRH-II and its receptor regulate porcine sperm function in an autocrine/paracrine manner.</description><identifier>ISSN: 0016-6480</identifier><identifier>ISSN: 1095-6840</identifier><identifier>EISSN: 1095-6840</identifier><identifier>DOI: 10.1016/j.ygcen.2024.114653</identifier><identifier>PMID: 39667624</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>agonists ; Animals ; antagonists ; autocrine signaling ; Biomechanical Phenomena ; boars ; Connecting piece ; GnRH-II ; GnRH-II receptor ; gonadotropin-releasing hormone ; Gonadotropin-Releasing Hormone - metabolism ; immunoblotting ; kinematics ; Male ; Porcine ; Receptors, LHRH - metabolism ; seminal plasma ; Sperm morphology ; Sperm motility ; Sperm Motility - drug effects ; Sperm Motility - physiology ; spermatids ; spermatogenesis ; Spermatozoa ; Spermatozoa - drug effects ; Spermatozoa - metabolism ; Spermatozoa - physiology ; Swine ; testes ; Testis - drug effects ; Testis - metabolism</subject><ispartof>General and comparative endocrinology, 2025-01, Vol.361, p.114653, Article 114653</ispartof><rights>2024</rights><rights>Copyright © 2024. Published by Elsevier Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c272t-43d6e3ad402b063c42326a8bcc12eac8b8ab57692ea32fe81da8618cc4af729c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0016648024002168$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39667624$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Desaulniers, A.T.</creatorcontrib><creatorcontrib>Ross, C.E.</creatorcontrib><creatorcontrib>Cederberg, R.A.</creatorcontrib><creatorcontrib>Lovercamp, K.W.</creatorcontrib><creatorcontrib>Lents, C.A.</creatorcontrib><creatorcontrib>White, B.R.</creatorcontrib><title>Gonadotropin-releasing hormone II and its receptor regulate motility, morphology, and kinematics of porcine spermatozoa in vitro</title><title>General and comparative endocrinology</title><addtitle>Gen Comp Endocrinol</addtitle><description>[Display omitted]
•In the boar reproductive tract, GnRH-II and GnRHR-II are most abundant within testes.•GnRHR-II immunolocalizes to elongating spermatids and the connecting piece of sperm.•Seminal plasma of mature boars contains GnRH-II.•GnRH-II analogues affect sperm motility, morphology and kinematics in extended semen.
The second form of gonadotropin-releasing hormone (GnRH-II) and its receptor (GnRHR-II) are abundantly produced within the porcine testis and immunolocalize within the seminiferous tubules, suggesting a role in spermatogenesis and/or sperm function. The objective of this study was to quantify GnRH-II and GnRHR-II abundance within boar reproductive tract tissues and examine their role in porcine sperm function. Immunoblotting revealed GnRHR-II abundance was 12-fold greater (P < 0.0001) within the testis compared with other reproductive organs. Within seminiferous tubules, GnRHR-II prominently immunolocalized to elongating spermatids. In ejaculated spermatozoa, GnRHR-II immunolocalized to the connecting piece. GnRH-II was also detected in seminal plasma, likely originating from the testis as GnRH-II concentrations were greatest in testicular homogenates (P < 0.0001) compared with other reproductive tissues. To assess the effects of GnRH-II/GnRHR-II on sperm function, extended semen samples were treated with GnRHR-II analogues and evaluated via computer-assisted sperm analysis (CASA). In Experiment 1, semen treatment with increasing concentrations of GnRHR-II agonist (D-ala6 GnRH-II) revealed that two concentrations (0.1 and 100 µM) tended to decrease the percentage of bent sperm tails versus vehicle-treated semen (P < 0.10). In Experiment 2, semen treatment with increasing concentrations of GnRHR antagonist (SB-75/Cetrorelix) indicated that only 10 µM SB-75 impaired CASA metrics compared with vehicle-treated samples (P < 0.05). In Experiment 3, semen treatment with both 100 µM D-ala6 GnRH-II and 10 µM SB-75 partially rescued sperm motility and morphology measures. These data suggest that GnRH-II and its receptor regulate porcine sperm function in an autocrine/paracrine manner.</description><subject>agonists</subject><subject>Animals</subject><subject>antagonists</subject><subject>autocrine signaling</subject><subject>Biomechanical Phenomena</subject><subject>boars</subject><subject>Connecting piece</subject><subject>GnRH-II</subject><subject>GnRH-II receptor</subject><subject>gonadotropin-releasing hormone</subject><subject>Gonadotropin-Releasing Hormone - metabolism</subject><subject>immunoblotting</subject><subject>kinematics</subject><subject>Male</subject><subject>Porcine</subject><subject>Receptors, LHRH - metabolism</subject><subject>seminal plasma</subject><subject>Sperm morphology</subject><subject>Sperm motility</subject><subject>Sperm Motility - drug effects</subject><subject>Sperm Motility - physiology</subject><subject>spermatids</subject><subject>spermatogenesis</subject><subject>Spermatozoa</subject><subject>Spermatozoa - drug effects</subject><subject>Spermatozoa - metabolism</subject><subject>Spermatozoa - physiology</subject><subject>Swine</subject><subject>testes</subject><subject>Testis - drug effects</subject><subject>Testis - metabolism</subject><issn>0016-6480</issn><issn>1095-6840</issn><issn>1095-6840</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2025</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc1uEzEURi0EoqHwBEjISxZM8N_ccRYsUAUlUiU2sLY8njupw4w92E6lsOqj45DSJWLlz1fn-pN8CHnN2ZozDu_36-POYVgLJtSacwWtfEJWnG3aBrRiT8mKVawBpdkFeZHznjHWSuDPyYXcAHQg1IrcX8dgh1hSXHxoEk5osw87ehvTHAPS7ZbaMFBfMk3ocCkx1bA7TLYgnWPxky_HdzWl5TZOcVfzif_hA862eJdpHOkSk6sDmhdMdRp_RUt9oHe-1r4kz0Y7ZXz1cF6S758_fbv60tx8vd5efbxpnOhEaZQcAKUdFBM9A-mUkAKs7p3jAq3TvbZ928GmXqQYUfPBauDaOWXHTmycvCRvz-8uKf48YC5m9tnhNNmA8ZCN5NDqTgjd_geqAEC1XFRUnlGXYs4JR7MkP9t0NJyZkyWzN38smZMlc7ZUt948FBz6GYfHnb9aKvDhDGD9kTuPyWTnMTgcfLVQzBD9Pwt-AxsKprM</recordid><startdate>202501</startdate><enddate>202501</enddate><creator>Desaulniers, A.T.</creator><creator>Ross, C.E.</creator><creator>Cederberg, R.A.</creator><creator>Lovercamp, K.W.</creator><creator>Lents, C.A.</creator><creator>White, B.R.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope></search><sort><creationdate>202501</creationdate><title>Gonadotropin-releasing hormone II and its receptor regulate motility, morphology, and kinematics of porcine spermatozoa in vitro</title><author>Desaulniers, A.T. ; Ross, C.E. ; Cederberg, R.A. ; Lovercamp, K.W. ; Lents, C.A. ; White, B.R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c272t-43d6e3ad402b063c42326a8bcc12eac8b8ab57692ea32fe81da8618cc4af729c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2025</creationdate><topic>agonists</topic><topic>Animals</topic><topic>antagonists</topic><topic>autocrine signaling</topic><topic>Biomechanical Phenomena</topic><topic>boars</topic><topic>Connecting piece</topic><topic>GnRH-II</topic><topic>GnRH-II receptor</topic><topic>gonadotropin-releasing hormone</topic><topic>Gonadotropin-Releasing Hormone - metabolism</topic><topic>immunoblotting</topic><topic>kinematics</topic><topic>Male</topic><topic>Porcine</topic><topic>Receptors, LHRH - metabolism</topic><topic>seminal plasma</topic><topic>Sperm morphology</topic><topic>Sperm motility</topic><topic>Sperm Motility - drug effects</topic><topic>Sperm Motility - physiology</topic><topic>spermatids</topic><topic>spermatogenesis</topic><topic>Spermatozoa</topic><topic>Spermatozoa - drug effects</topic><topic>Spermatozoa - metabolism</topic><topic>Spermatozoa - physiology</topic><topic>Swine</topic><topic>testes</topic><topic>Testis - drug effects</topic><topic>Testis - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Desaulniers, A.T.</creatorcontrib><creatorcontrib>Ross, C.E.</creatorcontrib><creatorcontrib>Cederberg, R.A.</creatorcontrib><creatorcontrib>Lovercamp, K.W.</creatorcontrib><creatorcontrib>Lents, C.A.</creatorcontrib><creatorcontrib>White, B.R.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>General and comparative endocrinology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Desaulniers, A.T.</au><au>Ross, C.E.</au><au>Cederberg, R.A.</au><au>Lovercamp, K.W.</au><au>Lents, C.A.</au><au>White, B.R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Gonadotropin-releasing hormone II and its receptor regulate motility, morphology, and kinematics of porcine spermatozoa in vitro</atitle><jtitle>General and comparative endocrinology</jtitle><addtitle>Gen Comp Endocrinol</addtitle><date>2025-01</date><risdate>2025</risdate><volume>361</volume><spage>114653</spage><pages>114653-</pages><artnum>114653</artnum><issn>0016-6480</issn><issn>1095-6840</issn><eissn>1095-6840</eissn><abstract>[Display omitted]
•In the boar reproductive tract, GnRH-II and GnRHR-II are most abundant within testes.•GnRHR-II immunolocalizes to elongating spermatids and the connecting piece of sperm.•Seminal plasma of mature boars contains GnRH-II.•GnRH-II analogues affect sperm motility, morphology and kinematics in extended semen.
The second form of gonadotropin-releasing hormone (GnRH-II) and its receptor (GnRHR-II) are abundantly produced within the porcine testis and immunolocalize within the seminiferous tubules, suggesting a role in spermatogenesis and/or sperm function. The objective of this study was to quantify GnRH-II and GnRHR-II abundance within boar reproductive tract tissues and examine their role in porcine sperm function. Immunoblotting revealed GnRHR-II abundance was 12-fold greater (P < 0.0001) within the testis compared with other reproductive organs. Within seminiferous tubules, GnRHR-II prominently immunolocalized to elongating spermatids. In ejaculated spermatozoa, GnRHR-II immunolocalized to the connecting piece. GnRH-II was also detected in seminal plasma, likely originating from the testis as GnRH-II concentrations were greatest in testicular homogenates (P < 0.0001) compared with other reproductive tissues. To assess the effects of GnRH-II/GnRHR-II on sperm function, extended semen samples were treated with GnRHR-II analogues and evaluated via computer-assisted sperm analysis (CASA). In Experiment 1, semen treatment with increasing concentrations of GnRHR-II agonist (D-ala6 GnRH-II) revealed that two concentrations (0.1 and 100 µM) tended to decrease the percentage of bent sperm tails versus vehicle-treated semen (P < 0.10). In Experiment 2, semen treatment with increasing concentrations of GnRHR antagonist (SB-75/Cetrorelix) indicated that only 10 µM SB-75 impaired CASA metrics compared with vehicle-treated samples (P < 0.05). In Experiment 3, semen treatment with both 100 µM D-ala6 GnRH-II and 10 µM SB-75 partially rescued sperm motility and morphology measures. These data suggest that GnRH-II and its receptor regulate porcine sperm function in an autocrine/paracrine manner.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>39667624</pmid><doi>10.1016/j.ygcen.2024.114653</doi></addata></record> |
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| subjects | agonists Animals antagonists autocrine signaling Biomechanical Phenomena boars Connecting piece GnRH-II GnRH-II receptor gonadotropin-releasing hormone Gonadotropin-Releasing Hormone - metabolism immunoblotting kinematics Male Porcine Receptors, LHRH - metabolism seminal plasma Sperm morphology Sperm motility Sperm Motility - drug effects Sperm Motility - physiology spermatids spermatogenesis Spermatozoa Spermatozoa - drug effects Spermatozoa - metabolism Spermatozoa - physiology Swine testes Testis - drug effects Testis - metabolism |
| title | Gonadotropin-releasing hormone II and its receptor regulate motility, morphology, and kinematics of porcine spermatozoa in vitro |
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